Journal
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
Volume 56, Issue 3, Pages 187-192Publisher
MICROBIOL RES FOUNDATION
DOI: 10.2323/jgam.56.187
Keywords
Escherichia coli; fluorescence-activated cell sorting (FACS); green fluorescent protein (GFP); H-NS; insertion sequence; IS1; transposition
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Funding
- Ministry of Education, Culture, Sports, Science and Technology of Japan
- Japan Society for the Promotion of Science [17GS0314]
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Transposons play a significant role in the evolution of bacterial genomes. Quantifying frequency of transpositional events caused by a transposon will facilitate understanding its role. Here, we report successful measurement of the frequency of IS1 transposition using GFP hop-on assay in which transposition-dependent GFP expression is monitored by FACS. This assay allows easy assessment of IS transposition into the chromosomal DNA on a single-cell scale; this is an advantage over other conventional methods to measure transposition frequency.
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