Review
Biochemistry & Molecular Biology
Betul Celik, Kader Cicek, Andres Felipe Leal, Shunji Tomatsu
Summary: Osteosarcoma is the most common malignant bone tumor affecting adolescents and young adults, requiring the development of novel targeted therapies for relapse cases. RNA interference technologies show promise in cancer treatment by silencing overexpressed genes to induce apoptosis in tumor cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dapeng Wang, Jianbo Xiu, Jiangyue Zhao, Junli Luo
Summary: In this study, a new method called miR-AB was developed for simplified, cost-effective, and error-proof production of shRNAmirs. It was successfully applied in loss-of-function experiments for multiple genes at a single-cell level, providing a powerful tool for uncovering the functions of genes in complex biological processes.
Article
Biochemistry & Molecular Biology
Jinmo Koo, Dhandapani Gurusamy, Subba Reddy Palli
Summary: There is limited success in using exogenous small interfering RNA (siRNA) or small hairpin RNA (shRNA) to trigger RNA interference (RNAi) in insects. Instead, long double-stranded RNA (dsRNA) is used to induce gene knockdown in insects. In this study, the potency of si/sh RNAs and dsRNA was compared in Colorado potato beetle (CPB) cells. CPB cells showed a highly efficient RNAi response to dsRNA, but si/sh RNAs were inefficient in triggering RNAi in CPB cells.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Review
Oncology
Ting Wang, Yunhong Yao, Xinrong Hu, Yi Zhao
Summary: The article introduces gene editing interference technology and its potential applications in clinical treatment. It focuses on the mechanisms of CRISPRi, RNAi, and LncRNA interference, and describes their applications and differences in adenocarcinoma research.
Article
Microbiology
Qun Wang, Qi Su, Bowen Liu, Yan Li, Wanli Sun, Yanxue Liu, Ruyu Xue, Shuang Chang, Yixin Wang, Peng Zhao
Summary: This study developed a treatment method for combating ALV in vitro culture system by using a combination of AZT and shRNA targeting ALV. The results showed that this method significantly improved antiviral efficacy with low cytotoxicity.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Medicine, Research & Experimental
Hsing-Chuan Tsai, Violena Pietrobon, Maoyu Peng, Suning Wang, Lihong Zhao, Francesco M. Marincola, Qi Cai
Summary: Regulating gene expression is crucial for treating genetic diseases, and several molecular tools have been developed for this purpose. This review discusses the different types of molecular tools, their delivery methods, and recent advancements in the field.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Veterinary Sciences
Elaheh Amjadimanesh, Azam Mokhtari, Behnaz Saffar
Summary: In this study, an RNAi-based antiviral approach targeting BHV1-UL25 gene was designed. The results demonstrated that this method effectively suppressed the replication and expression of BHV-1 virus, showing significant antiviral attributes.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Endocrinology & Metabolism
Anna L. Gloyn, Mark Ibberson, Piero Marchetti, Alvin C. Powers, Patrik Rorsman, Maike Sander, Michele Solimena
Summary: This Perspective discusses the current status and challenges in human islet research, emphasizing the importance of standardization to address analytical challenges and promote international collaboration. The authors propose actions to accelerate research progress and increase understanding of type 2 diabetes.
Article
Endocrinology & Metabolism
Selina Wrublewsky, Thimoteus Speer, Lisa Nalbach, Anne S. Boewe, Mandy Pack, Dalia Alansary, Leticia P. Roma, Markus D. A. Hoffmann, Beate M. Schmitt, Andrea Weinzierl, Michael D. Menger, Matthias W. Laschke, Emmanuel Ampofo
Summary: The inhibition of NLRP3 improves the survival and endocrine function of pancreatic islet grafts by enhancing revascularization and inducing insulin gene expression. This inhibition has significant therapeutic potential in pancreatic islet transplantation.
Article
Cell Biology
Anna Campanati, Miriam Caffarini, Federico Diotallevi, Giulia Radi, Guendalina Lucarini, Mariangela Di Vincenzo, Monia Orciani, Annamaria Offidani
Summary: The study suggests that Apremilast can normalize the abnormal pattern of Psoriasis MSCs by regulating the expression of VEGF, iNOS, and IDO. Furthermore, Apremilast reduces the expression of VEGF, iNOS, and IDO in skin sections, which contributes to the treatment of psoriasis.
INFLAMMATION RESEARCH
(2021)
Article
Medicine, Research & Experimental
Lisa Nalbach, Leticia P. Roma, Beate M. Schmitt, Vivien Becker, Christina Korbel, Selina Wrublewsky, Mandy Pack, Thomas Spater, Wolfgang Metzger, Maximilian M. Menger, Florian S. Frueh, Claudia Gotz, Haopeng Lin, Joseline EM Fox, Patrick E. MacDonald, Michael D. Menger, Matthias W. Laschke, Emmanuel Ampofo
Summary: Prevascularized islet organoids with functional microvessels can improve the success rates of clinical islet transplantation, as they exhibit higher angiogenic activity and can be rapidly blood-perfused after transplantation.
EMBO MOLECULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Xiaojie Fan, Jingnan Yang, Guangyao Wu, Meiyi Wang, Xiaoxia Cheng, Chang Liu, Qian Liu, Yanan Wen, Shuangshuang Meng, Zhenxing Wang, Xuhong Lin, Lei An
Summary: In this study, we optimized the conditions for RNA interference (RNAi) using the EZ Trans Cell Reagent and successfully conducted the RNAi experiment. Our data will be valuable for future RNAi research.
GENETICS AND MOLECULAR BIOLOGY
(2022)
Editorial Material
Cell Biology
Laura Crisa, Vincenzo Cirulli
Summary: In their recent paper, Chang and colleagues successfully achieved durable mixed hematopoietic chimerism that promotes the engraftment and long-term function of pancreatic islet allotransplants in fully immunocompetent mice without the need for immunosuppression.
Article
Engineering, Biomedical
Alexander E. Vlahos, Ilana Talior-Volodarsky, Sean M. Kinney, Michael V. Sefton
Summary: This study highlights a biomaterial-based approach using a methacrylic acid copolymer coating to generate a robust pre-vascularized subcutaneous cavity for islet transplantation. The pre-vascularized site was able to support a marginal mass of islets, rapidly returning streptozotocin-induced diabetic mice to normoglycemia.
Article
Endocrinology & Metabolism
Joana R. N. Lemos, David A. Baidal, Raffaella Poggioli, Virginia Fuenmayor, Carmen Chavez, Ana Alvarez, Elina Linetsky, Franck Mauvais-Jarvis, Camillo Ricordi, Rodolfo Alejandro
Summary: This study analyzed a cohort of islet transplantation recipients followed up for up to 20 years and evaluated the impact of sex on graft survival. The results showed that recipients of islets from at least one female donor had prolonged graft survival, and female recipients exhibited longer survival compared to male recipients following transplantation of at least one female donor.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)