Journal
JOURNAL OF FOOD SCIENCE
Volume 76, Issue 1, Pages C168-C177Publisher
WILEY
DOI: 10.1111/j.1750-3841.2010.01918.x
Keywords
comprehensive 2D GC-MS; fermented cucumber volatiles; GCxGC-TOFMS; metabolites; nontargeted data analysis; 2-dimensional gas chromatography
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Funding
- Pickle Packers Intl. Inc., Washington, D.C., U.S.A.
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A nontargeted, comprehensive 2-dimensional gas chromatography-time-of-flight mass spectrometry (GCxGC-TOFMS) method was developed for the analysis of fermented cucumber volatiles before and after anaerobic spoilage. Volatile compounds extracted by solid-phase microextraction were separated on a polyethylene glycol 1st-dimension column and 14% cyanopropylphenyl 2nd-dimension column. Among 314 components detected in fermented cucumber brine, 199 had peak areas with coefficients of variation below 30%. Peak identifications established by mass spectral library matching were 92% accurate based on 63 authentic standards. Analysis of variance of analytes' log peak areas revealed 33 metabolites changed in concentration after spoilage (P < 0.05), including increases in acetic, propanoic, and butyric acids, n-propyl acetate, several alcohols, and a decrease in furfural. GCxGC-TOFMS with a nontargeted, semi-automated approach to data analysis made possible the separation, identification, and determination of differences in polar volatile components, facilitating the discovery of several metabolites related to fermented cucumber spoilage.
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