Article
Biotechnology & Applied Microbiology
Kohei Takeoka, Hiroki Abe, Kento Koyama, Shigenobu Koseki
Summary: The study focused on Campylobacter jejuni survival dynamics in foodborne diseases, pH changes during simulated gastric digestion, and the predictability of C. jejuni survival in a model stomach. It was found that gastric digestion had limited impact on inactivating C. jejuni.
Article
Food Science & Technology
Daniel Eriksson, Ella Rahlen, Emma Bergenkvist, Moa Skarin, Lise-Lotte Fernstrom, Jesper Ryden, Ingrid Hansson
Summary: Most Campylobacter infections in humans are sporadic cases connected to private households. Chicken meat, believed to be the main source of human exposure, poses cross-contamination risks in the kitchen. Freezing can reduce Campylobacter concentrations on chicken meat. Different Campylobacter sequence types have varying abilities to withstand freezing stress. Campylobacter remaining on hands after washing and on cutting boards after wiping are likely sources of cross-contamination in the kitchen.
Article
Microbiology
Anand B. Karki, Bhuwan Khatri, Mohamed K. Fakhr
Summary: RNA-seq was used to analyze the differentially expressed genes (DEGs) in C. coli HC2-48 and C. jejuni OD2-67 transcriptomes under different temperature conditions. In C. coli HC2-48, genes involved in flagellar assembly were significantly impacted by low temperatures (4°C), while genes related to the ribosome and ribonucleoprotein complex were modulated in C. jejuni OD2-67 at 4°C. Although more DEGs were observed in the chicken juice medium compared to Mueller-Hinton broth, there were no common genes expressed at all incubation times, indicating that the food matrix environment is not the sole determinant of differential expression in Campylobacter spp. at low temperatures.
Article
Food Science & Technology
A. Arun Prince Milton, Kasanchi M. Momin, G. Bhuvana Priya, Samir Das, M. Angappan, Arnab Sen, D. K. Sinha, Sandeep Ghatak
Summary: A simple saltatory rolling circle amplification (SRCA) assay has been developed for rapid and accurate detection of Campylobacter jejuni in chicken meat. The specificity and sensitivity of the assay have been validated, showing 10- and 100-fold better detection limits compared to end-point and real-time PCR, respectively. This method is suitable for resource-limited food analysis laboratories.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Janani Govindaswamy, Veronique Zeller-Peronnet, Melanie Pavlovic, Daniela Wirtz, Larissa Murr, Diana Thaerigen, Bertrand Colson, Steffen Uhlig, Ulrich Busch, Ingrid Huber
Summary: In this study, a droplet digital PCR combined with propidium monoazide (PMA) treatment was established for quantifying C. coli and C. jejuni in chicken meat rinses. This method showed good accuracy, relative trueness, and selectivity, and demonstrated a strong correlation with the reference method.
APPLIED SCIENCES-BASEL
(2022)
Article
Food Science & Technology
Masaru Usui, Sho Tateno, Masanobu Onozaki, Naoaki Misawa, Yasuhiko Suzuki, Yutaka Tamura, Chie Nakajima
Summary: The rapid method CAMERA can simultaneously detect C. jejuni/coli and determine their susceptibility to fluoroquinolone/macrolide drugs. This study demonstrated the accuracy and efficiency of CAMERA in detecting and confirming antimicrobial resistance, providing a valuable tool for surveillance and control of resistant C. jejuni/coli contamination.
Article
Microbiology
Harald Nothaft, Maria Elisa Perez-Munoz, Tianfu Yang, Abarna V. M. Murugan, Michelle Miller, Daniel Kolarich, Graham S. Plastow, Jens Walter, Christine M. Szymanski
Summary: The study shows that Campylobacter jejuni spreads through contaminated poultry products, and an attenuated Escherichia coli live vaccine strain expressing the C. jejuni N-glycan can reduce the Campylobacter load in poultry, with distinct responder and non-responder outcomes. Research indicates that antibodies from responder birds have higher Campylobacter-opsonophagocytic activity, and transplantation of cecal microbiota from responder birds can enhance vaccine-induced antigen-specific IgY responses.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Jeong In Hur, Jinshil Kim, Sangryeol Ryu, Byeonghwa Jeon
Summary: This study demonstrates the existence of phylogenetic clusters in Campylobacter jejuni associated with cold stress tolerance and identifies the genetic element cfrA as a contributor to cold stress tolerance.
MICROBIOLOGY SPECTRUM
(2022)
Review
Immunology
Se-Hun Kim, Ramachandran Chelliah, Sudha Rani Ramakrishnan, Ayyappasamy Sudalaiyadum Perumal, Woo-Suk Bang, Momna Rubab, Eric Banan-Mwine Daliri, Kaliyan Barathikannan, Fazle Elahi, Eunji Park, Hyeon Yeong Jo, Su-Bin Hwang, Deog Hwan Oh
Summary: Campylobacter spp. are the leading cause of bacterial colon infections in humans globally, and their stress-adaptation mechanisms differ from other enteropathogenic bacteria, allowing them to survive in diverse environments and enhance their resistance mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Review
Immunology
Abdi Elmi, Fauzy Nasher, Nick Dorrell, Brendan Wren, Ozan Gundogdu
Summary: Research on Campylobacter jejuni has made significant progress, revealing emerging virulence factors that influence its pathogenicity. The spatial and temporal dynamics play a key role in promoting the bacterium to sense, adapt, and survive in multiple hosts. Future research should focus on obtaining a comprehensive understanding of C. jejuni virulence mechanisms.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Food Science & Technology
A. A. P. Milton, Kasanchi M. Momin, G. Bhuvana Priya, Samir Das, M. Angappan, Arnab Sen, D. K. Sinha, Sandeep Ghatak
Summary: A novel PSR assay was developed for rapid and sensitive screening of C. jejuni contamination in chicken samples, demonstrating high sensitivity and field applicability. This method could be a valuable tool for real-time monitoring of food safety, offering rapid detection without the need for specialized training or equipment.
Article
Engineering, Chemical
Ali Al-Sakkaf
Summary: This study aimed to determine the microbial kinetic parameters in chicken under dynamic temperature for campylobacteriosis strains in New Zealand. The obtained D and z values were consistent with international isothermal data, indicating that the high rate of campylobacteriosis in New Zealand is not due to the emergence of more heat resistant strains.
JOURNAL OF FOOD ENGINEERING
(2021)
Article
Veterinary Sciences
Chalita Jainonthee, Warangkhana Chaisowwong, Phakamas Ngamsanga, Anuwat Wiratsudakul, Tongkorn Meeyam, Duangporn Pichpol
Summary: In this study, a simple and rapid detection assay called LAMP was used to test for Campylobacter jejuni contamination in food processing plants. The LAMP assay showed high sensitivity and specificity, and was able to detect C. jejuni at low concentrations.
VETERINARY SCIENCES
(2022)
Review
Chemistry, Applied
Murat Gurbuz, Burcu Irem Omurtag Korkmaz
Summary: Poultry is a rapidly growing industry, but concerns about food safety and the presence of bacteria like Campylobacter in poultry remain. The discovery of new antimicrobials, such as eugenol, is important for ensuring the safety of poultry meat. However, further research is needed to determine the specific applications and effectiveness of eugenol as a food preservative in poultry meat safety.
Article
Biotechnology & Applied Microbiology
Taketoshi Iwata, Ayako Watanabe-Yanai, Yukino Tamamura-Andoh, Nobuo Arai, Masato Akiba, Masahiro Kusumoto
Summary: Campylobacter jejuni is a leading cause of foodborne bacterial gastroenteritis worldwide, and raw or undercooked chicken meat is considered the major source of human campylobacteriosis. We identified 36 compounds with inhibitory effects on C. jejuni, and among them, tryptanthrin (TRP) showed bactericidal activity against C. jejuni. TRP can effectively reduce the level of C. jejuni infection in chicken farms.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)