Article
Food Science & Technology
Ondrej Holy, Julio Parra-Flores, Sarah Lepuschitz, Maria Paula Alarcon-Lavin, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Jetsi Mancilla-Rojano, Werner Ruppitsch, Stephen Forsythe
Summary: This study investigated the putative virulence factors of six Cronobacter sakazakii strains isolated from powdered milk, identifying different sequence types and resistance genes. Some strains demonstrated high adherence and invasion abilities, while the cpa gene was not detected. Various virulence and resistance genes, as well as plasmids, were identified through whole-genome sequencing. Additionally, CRISPR matrices were observed in all strains, enhancing decision-making for health authorities to protect public health.
Article
Food Science & Technology
Shuyan Wu, Pornchanok Subharat, Gale Brightwell
Summary: The study found that the combination of blue light LED and external hydrogen peroxide (H2O2) can significantly reduce the number of Cronobacter sakazakii cells, especially for stationary phase and biofilm cells.
Article
Food Science & Technology
Haoran Wang, Yulu Li, Zhuo Li, Run Ma, Xiangyang Bai, Xiangjun Zhan, Kunyao Luo, Ruiying Su, Xuejiao Li, Xiaodong Xia, Chao Shi
Summary: This study found that Litsea cubeba essential oil (LC-EO) has antimicrobial activity against Cronobacter sakazakii strains, and its mechanism involves increasing reactive oxygen species production, compromising cell membrane integrity, and causing cellular damage. Additionally, LC-EO effectively inhibited C. sakazakii biofilms and showed potential as a natural antimicrobial agent in the dairy industry.
Article
Food Science & Technology
Babak Pakbin, Wolfram Manuel Bruck, Samaneh Allahyari, John W. A. Rossen, Razzagh Mahmoudi
Summary: This study investigated the prevalence, genotypic and phenotypic antibiotic resistance profile, and clonal relatedness of C. sakazakii strains isolated from powdered infant formula milk (PIFM) samples collected in Tehran, Iran. The results showed a high prevalence rate of C. sakazakii in PIFM samples, with high resistance to multiple antibiotics and a diverse genetic profile. The study highlights the need for novel microbial surveillance systems to control the contamination of this foodborne pathogen in infant foods.
Article
Microbiology
Xin Gan, Menghan Li, Shaofei Yan, Xiaofei Wang, Wei Wang, Fengqin Li
Summary: The study found that Cronobacter sakazakii from powdered infant formula manufacturing factories in China are mostly pan-susceptible with weak biofilm-forming abilities. The isolates, mostly ST64, exhibited clinical relevance and shared a close genetic background, indicating possible cross-contamination events between processing rooms and external environments.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Xin Gan, Menghan Li, Jin Xu, Shaofei Yan, Wei Wang, Fengqin Li
Summary: This study investigated the prevalence, antimicrobial resistance, virulence factors, and genomic environments of Cronobacter isolated from powdered infant formula and supplementary food in China. Two multidrug-resistant Cronobacter sakazakii isolates were identified, with genomic analysis suggesting acquisition of resistance genes through horizontal gene transfer.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Mohamed E. El Zowalaty, Linda Falgenhauer, Stephen Forsythe
Summary: The study presents the first draft genome sequence of C. sakazakii strain MEZCS99 isolated from a healthy chicken in South Africa, providing insights into its genetic characteristics and serving as a reference point for future molecular epidemiological studies on livestock-associated C. sakazakii.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Biotechnology & Applied Microbiology
Alfred Ke, Valeria R. Parreira, Jeffrey M. Farber, Lawrence Goodridge
Summary: This study aimed to select a potential synbiotic to inhibit the growth of Cronobacter sakazakii in an in vitro model. Six lactobacillus strains were chosen as potential synbiotic components based on their inhibitory properties and prebiotic metabolism. This study provides valuable information for the development of a synbiotic for powdered infant formula.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Infectious Diseases
Basma Badawy, Mayada Gwida, Asmaa Sadat, Marwa EL-Toukhy, Mohamed Sayed-Ahmed, Nawazish Alam, Sarfaraz Ahmad, Md Sajid Ali, Mahmoud Elafify
Summary: This study aimed to detect the prevalence and antimicrobial resistance of Listeria monocytogenes and Cronobacter sakazakii in dairy households and dried milk, as well as evaluate the antimicrobial effect of rose water, rose, and orange essential oils. The results showed that L. monocytogenes had a prevalence in all households, while C. sakazakii was only identified in one household. L. monocytogenes strains exhibited high resistance to multiple antibiotics, while all C. sakazakii strains were susceptible to the tested antibiotics. Among the essential oils, orange oil showed the strongest bactericidal activity.
Article
Microbiology
Chansom Keo-Oudone, Koudkeo Phommachan, Orathai Suliya, Mochamad Nurcholis, Somchanh Bounphanmy, Tomoyuki Kosaka, Mamoru Yamada
Summary: A strain of Cronobacter sakazakii OX-25 isolated from thermotolerant microbes in Laos has been found to efficiently convert xylose and glucose to 2,3-butanediol at high temperatures, demonstrating potential for industrial applications as a platform chemical.
Article
Food Science & Technology
Yanpeng Yang, Sheng Ma, Kailun Guo, Du Guo, Jiahui Li, Muxue Wang, Yutang Wang, Chunling Zhang, Xiaodong Xia, Chao Shi
Summary: This study found that combined LED illumination and citral treatment can effectively reduce the abundance of C. sakazakii in reconstituted powdered infant formula. The combination treatment also destroys cellular morphology and membrane integrity, and induces lipid peroxidation. Neither LED illumination nor citral treatment results in DNA breakdown in C. sakazakii.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Doyeon Kim, Jinwoo Kim, Minsik Kim
Summary: This study demonstrates the enhanced bactericidal activity of engineered lysins fused with amphipathic antimicrobial peptides against Gram-negative bacterium Cronobacter sakazakii. The fusion lysins are capable of disrupting bacterial cells and significantly reducing the risk of infection within a certain range of temperature and pH. Additionally, the study also shows the bactericidal effect of these fusion lysins against other Gram-negative pathogens.
Article
Microbiology
Jinchun Xu, Huangbing Yao, Yali Li, Qiaoming Liao, Xiaoxiao Wan, Lulu Liu, Xiaojing Ma, Han Tao, Hui-Li Wang, Yi Xu
Summary: In this study, antimicrobial photodynamic inactivation (aPDI) was used to inactivate Cronobacter sakazakii. The results showed that aPDI reduced the survival rate of C. sakazakii by 3 to 4 log. The mechanism of photokilling involved the damage to cell membrane integrity, as well as the disruption of biofilm formation. Additionally, the study found that aPDI affected the regulation of the CpxRA-OmpC pathway.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
R. Chauhan, W. Azmi, S. Bansal, G. Goel
Summary: This study demonstrated the antimicrobial activity of ferulic acid and p-coumaric acid against stressed and unstressed Cronobacter sakazakii in laboratory media and reconstituted powdered infant formulation. Ferulic acid and p-coumaric acid showed marked antibacterial activity with minimum inhibitory concentration in the range of 2.5-5 mg ml(-1) for unstressed C. sakazakii cells and a maximum inhibition at 20 mg ml(-1) in reconstituted PIF. Desiccation resulted in increased susceptibility to phenolic compounds.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Infectious Diseases
Ondrej Holy, Julio Parra-Flores, Jaroslav Bzdil, Adriana Cabal-Rosel, Beatriz Daza-Prieto, Ariadnna Cruz-Cordova, Juan Xicohtencatl-Cortes, Ricardo Rodriguez-Martinez, Sergio Acuna, Stephen Forsythe, Werner Ruppitsch
Summary: The objective of this study was to screen for antibiotic resistance, fitness and virulence genes in Cronobacter sakazakii strains isolated from food and powdered-milk-producing environments using whole-genome sequencing. Antibiotic-resistance genes and virulence genes were detected using various tools, and susceptibility testing was performed. The strains showed resistance to cephalotin and ampicillin, and multiple ARGs and VGs were identified. These findings suggest a potential risk of infection in susceptible populations.
Article
Biotechnology & Applied Microbiology
Gemechu G. Abdi, Yetenayet B. Tola, Chala G. Kuyu
Summary: This study evaluated the quality of honey in the supply chain in southwest Ethiopia and found significant differences in physicochemical and microbial quality among different actors. The study also developed a predictive model to detect adulteration, which showed good linearity and predictive capacity.
JOURNAL OF FOOD PROTECTION
(2024)
Article
Biotechnology & Applied Microbiology
Kathleen A. Glass, Jie Yin Lim, Quinn L. Singer
Summary: Commercial cheese brines can be a reservoir for salt-tolerant pathogens. This study found that the addition of hydrogen peroxide can effectively reduce the population of L. monocytogenes in cheese brines, especially at higher temperatures and salt concentrations. The presence of indigenous microorganisms may neutralize the effect of hydrogen peroxide.
JOURNAL OF FOOD PROTECTION
(2024)
