Journal
JOURNAL OF FOOD PROTECTION
Volume 75, Issue 6, Pages 1090-1098Publisher
INT ASSOC FOOD PROTECTION
DOI: 10.4315/0362-028X.JFP-11-456
Keywords
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Funding
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2011-0026113]
- Korea Ministry of Education, Science and Technology (Medical & Bio-Material Research Center)
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This study was designed to evaluate the effects of bile acid deconjugation by probiotic strains on the antibiotic susceptibility of antibiotic-sensitive and multiple antibiotic resistant Salmonella Typhimurium and Staphylococcus aureus. Eight probiotic strains, Bifidobacterium Ion gum B6, Lactobacillus acidophilus ADH, Lactobacillus brevis KACC 10553, Lactobacillus casei KACC 12413, Lactobacillus paracasei ATCC 25598, Lactobacillus rhamnosus GO, Leuconostoc mesenteroides KACC 12312, and Pediococcus acidilactici KACC 12307, were used to examine bile acid tolerance. The ability to deconjugate bile acids was evaluated using both thin-layer chromatography and high-perfomance liquid chromatography. The antibiotic susceptibility testing was carried out to determine the synergistic inhibitory activity of deconjugated bile acids. L. acidophilus, L. brevis, and P. acidilactici showed the most tolerance to the conjugated bile acids. P. acidilactici deconjugated glycocholic acid and glycodeoxycholate from 3.18 and 3.09 mM to the detection limits, respectively. The antibiotic susceptibility of selected foodborne pathogens was increased by increasing the concentration of deconjugated bile acids. The study results are useful for understanding the relationship between bile acid deconjugation by probiotic strains and antibiotic susceptibility in the presence of deconjugated bile acids, and they may be useful for designing new probiotic-antibiotic combination therapy based on bile acid deconjugation.
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