Article
Chemistry, Analytical
Guoxia Zheng, Qian Gao, Youwei Jiang, Ling Lu, Jianfeng Li, Xingcai Zhang, Hongyu Zhao, Panpan Fan, Yutong Cui, Furong Gu, Yunhua Wang
Summary: By combining digital microfluidic (DMF) with loop-mediated isothermal amplification (LAMP), an automated instrument-compact DMF-LAMP device was developed for rapid and sensitive detection of Vibrio parahaemolyticus. Proper mixing was found to enhance the amplification reaction rate, shorten the amplification time, and improve detection sensitivity of the device. The device showed high sensitivity with a detection limit of <0.23 x 10(3) CFU/g, making it a promising sample-to-answer system for quick and sensitive analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Yong Li, Shuo Lin, Yuxin Xue, Qianyue Jia, Ye Wang, Yingqiu Xie, Chao Shi, Cuiping Ma
Summary: BNNPs have been proven to significantly improve the specificity and sensitivity of PCR and LAMP amplification reactions, expanding the application range of nucleic acid detection and increasing detection sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biotechnology & Applied Microbiology
Xuexin Bai, Yanwei Li, Wenwen Xin, Lin Kang, Zhiying Jin, Wei Wan, Yue Li, Hao Yang, Jinglin Wang, Shan Gao
Summary: A rapid and sensitive immunochromatographic test paper assay was developed for the quantitative detection of thermostable direct hemolysin in seafood samples. The method can be completed within 15 minutes, has a detection limit below 50 ng/mL, and shows good specificity without cross-reactivity.
JOURNAL OF FOOD PROTECTION
(2023)
Article
Multidisciplinary Sciences
Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada
Summary: This study developed a RT-LAMP technique for the diagnosis of HPeVs, particularly HPeV3, allowing for rapid and convenient detection of HPeV3 RNA. The technique showed clinical utility in diagnosing newborns and infants with sepsis-like symptoms.
Article
Plant Sciences
Xiayan Pan, Xiao Wang, Junjie Yu, Mina Yu, Huijuan Cao, Mingli Yong, Tianqiao Song, Zhongqiang Qi, Yan Du, Rongsheng Zhang, Yongfeng Liu
Summary: In this study, a rapid LAMP assay was developed to detect the mating type of Villosiclava virens, the causal agent of rice false smut. The LAMP assay showed high sensitivity and specificity, with the ability to detect small amounts of DNA and provide results in just 45 minutes. It can serve as a rapid testing tool for mating type recognition of V. virens isolates in the field.
Article
Biotechnology & Applied Microbiology
L. I. A. N. X. I. A. Hu, S. H. U. F. E. Zhang, Y. U. L. I. N. G. Xue, J. U. N. H. U. A. Han, H. U. A. X. Yi, Y. U. E. H. U. A. Ke, Y. O. N. G. J. U. N. Xia, S. H. I. J. I. E. Wang
Summary: In this study, a real-time loop-mediated isothermal amplification (real-time LAMP) method was developed for the simultaneous detection of thermostable alkaline protease (TAP)-harboring Pseudomonas fluorescens. The method was specific, sensitive, rapid, and simple, and achieved a detection accuracy of 100% in raw milk samples.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Microbiology
Ahmad Mukhlis Abdul Rahman, Julian Ransangan, Vijay Kumar Subbiah
Summary: A rapid, simple, sensitive, and specific detection method based on loop-mediated isothermal amplification (LAMP) has been developed for the detection of Vibrio harveyi contamination. The addition of loop primers reduces the reaction time of LAMP by more than half, and with the use of SYBR Green, results can be obtained within 10 to 15 minutes without gel electrophoresis. LAMP has greater sensitivity compared to PCR.
Article
Biotechnology & Applied Microbiology
Yuan-qing Hu, Xian-hui Huang, Li-qing Guo, Zi-chen Shen, Lin-xue Lv, Feng-xia Li, Zan-hu Zhou, Dan-feng Zhang
Summary: Vibrio parahaemolyticus is a significant foodborne pathogen causing gastroenteritis in humans, with the bla(CARB-17) gene being a novel species-specific genetic marker. The LAMP-LFD assay developed in this study showed high specificity for detecting V. parahaemolyticus, with successful detection of the pathogen in contaminated and natural food samples. This method displayed a sensitivity 100-fold higher than conventional PCR, making it a suitable diagnostic tool for detecting V. parahaemolyticus in aquatic foods.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Qiqi Xue, Lingwei Sheng, Junjie Guo, Minghui Zou, Qiao Teng, Sijia Xu, Yuanyuan Li, Jinhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) method was successfully established for the detection of the Dermatophagoides farinae allergen. This method showed higher sensitivity, specificity, and efficiency compared to conventional PCR. It did not cross-react with other arthropod species commonly found in indoor dust. This study provides a potential model for the rapid detection of allergens produced by other house dust mites in the future.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Pratima Verma, Aakanksha Chauhan, Reena Thakur, Kusum Lata, Arpita Sharma, Kausik Chattopadhyay, Arunika Mukhopadhaya
Summary: This study elucidates the cell death response triggered by TDH and demonstrates that this cell death exhibits apoptotic characteristics. The research found that TDH induces mitochondrial damage, resulting in the release of AIF and endonuclease G, which execute caspase-independent cell death. Furthermore, the role of ROS in THP-1 cells and PARP-1 in Caco-2 cells in this process has also been highlighted.
MOLECULAR MICROBIOLOGY
(2023)
Article
Food Science & Technology
Xinran Xiang, Enjie Diao, Yuting Shang, Minghui Song, Yinglong He
Summary: With the rapid development of logistics, there is an urgent need to develop effective detection strategies to ensure food safety, as a growing number of pathogenic microorganisms spread worldwide using food as a carrier. This study developed a high-fidelity target-based microfluidic identification (HFTMI) strategy by combining novel markers identified by pan-genome analysis and a digital recombinase-aided amplification (RAA) detection method. The HFTMI system showed improved performance compared to qPCR, providing more reliable guidance for food safety testing.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Landon J. Getz, Justin M. Brown, Lauren Sobot, Alexandra Chow, Jastina Mahendrarajah, Nikhil A. Thomas
Summary: Pathogenic Vibrio species cause 3-5 million life-threatening human infections annually. The winged helix-turn-helix (wHTH) transcriptional regulator HlyU positively regulates the expression of bacterial hemolysin and toxin genes, while histone-like nucleoid structural protein (H-NS) silences their expression. HlyU is required for virulence gene expression associated with type 3 Secretion System-1 (T3SS1) in Vibrio parahaemolyticus, although its mechanism of action is not understood.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Analytical
Wenjuan Wu, Congcong Yin, Aiqin Yue, Jingping Niu, Weijun Du, Dingbin Liu, Jinzhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for visual and rapid detection of the major prevalent SMV strain in China. The assay showed high analytical performance and potential for early detection of SMV-SC7-associated plant diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Food Science & Technology
Xinrui Lv, Weiwei Cao, Huang Zhang, Yilin Zhang, Lei Shi, Lei Ye
Summary: A system combining chemically enhanced CRISPR and RAA technologies was established for detecting V. parahaemolyticus in seafood, showing rapid, accurate detection with no cross-reactivity.
Article
Food Science & Technology
Ruibin Xie, Hui Li, Wenjie Yu, Nan Wang, Juan Zhang, Jie Gao, Ailiang Chen
Summary: This study successfully identified Takifugu species using a rapid and visual loop-mediated isothermal amplification method. The method has high specificity and can prevent false labeling, protect consumer rights, and reduce the risk of pufferfish poisoning.
JOURNAL OF FOOD SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Gemechu G. Abdi, Yetenayet B. Tola, Chala G. Kuyu
Summary: This study evaluated the quality of honey in the supply chain in southwest Ethiopia and found significant differences in physicochemical and microbial quality among different actors. The study also developed a predictive model to detect adulteration, which showed good linearity and predictive capacity.
JOURNAL OF FOOD PROTECTION
(2024)
Article
Biotechnology & Applied Microbiology
Kathleen A. Glass, Jie Yin Lim, Quinn L. Singer
Summary: Commercial cheese brines can be a reservoir for salt-tolerant pathogens. This study found that the addition of hydrogen peroxide can effectively reduce the population of L. monocytogenes in cheese brines, especially at higher temperatures and salt concentrations. The presence of indigenous microorganisms may neutralize the effect of hydrogen peroxide.
JOURNAL OF FOOD PROTECTION
(2024)