Article
Microbiology
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study examined the prevalence and antimicrobial resistance of E. coli O157:H7 in lettuce in Addis Ababa, Ethiopia. The results indicated a low prevalence but presence of drug-resistant strains, highlighting a potential public health risk associated with this pathogen in the area.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Stephanie Arellano, Libin Zhu, Govindaraj Dev Kumar, Bibiana Law, Mendel Friedman, Sadhana Ravishankar
Summary: The antimicrobial activities of essential oil-based microemulsions in wash water against Escherichia coli O157:H7 and Pseudomonas fluorescens on Iceberg lettuce were investigated. The results showed that the 0.1% oregano oil microemulsion had the best effect in preventing the growth of E. coli and improving the quality of Iceberg lettuce.
Article
Acoustics
Qiao He, Donghong Liu, Muthupandian Ashokkumar, Xingqian Ye, Tony Z. Jin, Mingming Guo
Summary: This study explored the response of bacterial cell membranes to ultrasound exposure. It was found that ultrasonic treatment resulted in a significant reduction in E. coli populations and increased sensitivity to antimicrobial compounds. Additionally, ultrasonic-treated cells showed membrane damages and increased membrane permeability.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Food Science & Technology
Karin Hassenberg, Ulrike Praeger, Werner B. Herppich
Summary: Chlorine dioxide (ClO2) as a disinfectant in vegetable processing effectively reduces bacterial loads and improves product quality and consumer safety. Although the disinfection efficacy is limited for Listeria monocytogenes, it helps to prevent cross-contamination at high organic loads.
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Yu Zhang, Hung-Hsin Huang, Luyan Z. Ma, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto
Summary: The study evaluated the combined effects of phage FP43 and mild heated slightly acidic hypochlorous water (SAHW) in reducing established biofilms on lettuce contaminated with Escherichia coli O157:H7. The sequential treatment of phage and SAHW significantly decreased the number of adhered cells, but did not completely remove the biofilms. Bacterial regrowth was observed after treatment with SAHW alone. The combination of phage and SAHW can be considered as a promising strategy to control the formation of E. coli O157:H7 biofilms on lettuce.
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Food Science & Technology
Kai Dong, Yuwan Li, Sijia Luo, Fan Zhang, Hanxu Pan, Liang Zhao, Yongtao Wang, Xiaojun Liao
Summary: The study analyzed the sterilizing effects of different HHP-boosting processes on Escherichia coli O157:H7, finding that linear and fast boosting had the most significant bactericidal effects. The proteins interacting with GroEL under different boost modes and rates were involved in 42 metabolic pathways, particularly ribosome, ABC transporter, oxidative phosphorylation, sulfate assimilation, and cysteine biosynthesis. Key interacting proteins NuoB, NuoF, CysD, ZnuC, and RplX were identified and their interactions with GroEL were verified in vivo.
Article
Chemistry, Analytical
Manman Du, Jinbiao Ma, Zhiwei Zhang, Guangzu Wu, Jianguo Wu, Hao Wang, Xinwu Xie, Can Wang
Summary: This study developed a rGO-FET biosensor for the rapid and sensitive detection of pathogenic microorganisms. The biosensor demonstrated good specificity, a wide linear range, and the ability to directly detect real samples without pretreatment.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biophysics
Yaqian Chen, Jiaqi Ma, Xuechi Yin, Ziai Deng, Xiaojing Liu, Di Yang, Lei Zhao, Jing Sun, Jianlong Wang, Daohong Zhang
Summary: An immersible amplification dip-stick immunoassay (DSIA) using multifunctional nanocomposites was developed for the sensitive detection of Salmonella typhimurium (S. typhi) and Escherichia coli O157:H7 (E. coli O157:H7). The reporter molecule, CoFe2O4 (CFO), possessed superior catalase-like activity and magnetic properties. It enabled a simple and highly sensitive detection with a dual-visible mode. The DSIA achieved matrix-interference-free detection with a dynamic range of 102-108 CFU/mL and a low assay limit of 102 CFU/mL, and showed reasonable recovery rates for contamination monitoring in milk and beef samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Veterinary Sciences
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study estimated the prevalence and antimicrobial resistance of Escherichia coli O157:H7 in raw beef sold in butcher shops in Addis Ababa, Ethiopia. Out of the 384 samples examined, 3.64% carried E. coli O157:H7, with 57.14% of the isolates showing resistance to three or more antimicrobial agents. Thus, stricter monitoring of antimicrobial use and further studies on contamination sources and risk mitigation strategies are needed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Acoustics
Jiao Li, Donghong Liu, Tian Ding
Summary: The study investigated the gene regulation at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation, revealing significant differential expressions of 1217 genes, including up-regulated and down-regulated genes related to crucial biomolecular processes. The most enriched pathways were analyzed in carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription, and translation categories. Additionally, the study found that the citrate cycle genes were down-regulated, affecting carbohydrate decomposition and energy production under ultrasonic stress.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Food Science & Technology
Dianbo Zhao, Shaodan Wang, Yushan Hu, Xiao Liu, Jing Tao, Gianni Sagratini, Qisen Xiang
Summary: This study reveals the antibacterial mechanism of lauric arginate (LAE) against Escherichia coli. LAE damages the cytoplasmic and outer membranes of E. coli, causing a loss of membrane integrity. It also induces oxidative stress and increases reactive oxygen species levels. The combination of membrane damage and oxidative stress contributes to the antibacterial activity of LAE.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Food Science & Technology
Shefali Dobhal, Guodong Zhang, Dhiraj Gautam, Jacqueline Fletcher, Li Maria Ma
Article
Biotechnology & Applied Microbiology
Carter R. Newell, Michael Doyle, Li Ma
Article
Biochemical Research Methods
Chris Timmons, Eija Trees, Efrain M. Ribot, Peter Gerner-Smidt, Patti LaFon, Sung Im, Li Maria Ma
JOURNAL OF MICROBIOLOGICAL METHODS
(2016)
Article
Agriculture, Dairy & Animal Science
L. Hu, L. M. Ma, S. Zheng, X. He, H. Wang, E. W. Brown, T. S. Hammack, G. Zhang
Article
Food Science & Technology
Chris Timmons, Kedar Pai, Jamey Jacob, Guodong Zhang, Li Maria Ma
Article
Food Science & Technology
Lijun Hu, Li M. Ma, Shimin Zheng, Xiaohua He, Thomas S. Hammack, Eric W. Brown, Guodong Zhang
Article
Food Science & Technology
Lijun Hu, Xiaohong Deng, Eric W. Brown, Thomas S. Hammack, Li M. Ma, Guodong Zhang
Article
Food Science & Technology
Lijun Hu, Robert Stones, Eric W. Brown, Marc W. Allard, Li M. Ma, Guodong Zhang
Article
Multidisciplinary Sciences
Kedar Pai, Chris Timmons, Kevin D. Roehm, Alvin Ngo, Sai Sankara Narayanan, Akhilesh Ramachandran, Jamey D. Jacob, Li Maria Ma, Sundararajan Madihally
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Alejandro Penaloza-Vazquez, Li Maria Ma, Patricia Rayas-Duarte
CANADIAN JOURNAL OF MICROBIOLOGY
(2019)
Article
Agriculture, Dairy & Animal Science
Lijun Hu, Guojie Cao, Eric W. Brown, Marc W. Allard, Li M. Ma, Ashraf A. Khan, Guodong Zhang
Article
Biotechnology & Applied Microbiology
Claudia Diaz, Santiago Molina, Michael W. Smith, Charles Rohla, Li Maria Ma
Summary: Animals in produce production areas may contaminate foodborne pathogens on produce. This study investigated the impact of cattle grazing and waiting periods on the prevalence of Salmonella and STEC in native pecan production orchards. The results showed that the prevalence of both pathogens was higher in cattle-grazed orchards compared to nongrazed orchards. However, waiting periods did not have a significant impact on reducing the risk of contamination.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Multidisciplinary Sciences
Lijun Hu, Guojie Cao, Eric W. Brown, Marc W. Allard, Li M. Ma, Guodong Zhang
Summary: This study investigated the diversity of ten target genes commonly used in the detection and identification of Salmonella, finding 45 non-synonymous mutations across these genes. Phylogenetic analysis showed Salmonella ser. Typhimurium to be more diverse than Enteritidis or Heidelberg. This research provides insights that will aid in the development of sensitive molecular detection methods for Salmonella.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Li Maria Ma, Nicolas Lopez, Guodong Zhang
Summary: This article reports the draft whole-genome sequences of four Bacillus strains selected as potential probiotics for poultry. These genome sequences will provide a foundation for further characterization and understanding of their probiotic attributes.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2023)
Article
Physics, Fluids & Plasmas
Alvin D. Ngo, Kedar Pai, Christopher Timmons, Li Maria Ma, Jamey Jacob
Summary: This study evaluated cylindrical plasma actuators using surface dielectric barrier discharge (SDBD) for inactivation of Salmonella enterica. The results showed that an increase in induced airflow and improved mixing tendencies of plasma species led to higher bacterial inactivation efficiency. Therefore, enhancing particle velocity, airflow mixing tendencies, and plasma volume for the same power inputs can result in increased surface decontamination efficiency.
Article
Biotechnology & Applied Microbiology
Gemechu G. Abdi, Yetenayet B. Tola, Chala G. Kuyu
Summary: This study evaluated the quality of honey in the supply chain in southwest Ethiopia and found significant differences in physicochemical and microbial quality among different actors. The study also developed a predictive model to detect adulteration, which showed good linearity and predictive capacity.
JOURNAL OF FOOD PROTECTION
(2024)
Article
Biotechnology & Applied Microbiology
Kathleen A. Glass, Jie Yin Lim, Quinn L. Singer
Summary: Commercial cheese brines can be a reservoir for salt-tolerant pathogens. This study found that the addition of hydrogen peroxide can effectively reduce the population of L. monocytogenes in cheese brines, especially at higher temperatures and salt concentrations. The presence of indigenous microorganisms may neutralize the effect of hydrogen peroxide.
JOURNAL OF FOOD PROTECTION
(2024)