Journal
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
Volume 24, Issue 7, Pages 1073-1080Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.jfca.2011.01.010
Keywords
Antioxidants; HPLC post-column derivatization; ABTS; DPPH; Folin-Ciocalteu reagent; High performance liquid chromatography; Aronia; Food analysis; Food composition
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This study demonstrates the potential of a commercially available derivatization instrument coupled with HPLC for separation and on-line determination of antioxidants detected with widely used screening reagents DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonic acid)) or FCR (Folin-Ciocalteu's phenol reagent). As a result of optimization, key parameters of the analytical procedure were established: concentrations of derivatization reagents 1.5 mmol/L and 2.1 mmol/L in methanol for DPPH and ARTS, respectively, and 40% (v/v) FCR solution in water; reactor temperatures 130 degrees C for ARTS and FCR and 50 degrees C in the case of DPPH. In order to validate these procedures, Trolox equivalent antioxidant capacities (TEACs) for standard antioxidants obtained by post-column derivatization (on-line) and typical batch colorimetric methods (off-line) were compared; the corresponding measurements were found to be strongly correlated (Pearson coefficient: 0.973, 0.922, 0.853 for DPPH, ABTS and FCR, respectively). The results obtained for standard antioxidants and real fruit sample-aronia extract confirm the applicability of the proposed system to the on-line detection of antioxidants. Its great advantage compared to current routine methods is that it provides both chromatographic profiles and corresponding fingerprints of antioxidants (including unknown ones) along with quantitative determination of antioxidative potential-total, and those exhibited by individual compounds. (C) 2011 Elsevier Inc. All rights reserved.
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