Determination of glucosamine and lactose in milk-based formulae by high-performance liquid chromatography

A simple method for the determination of glucosamine and lactose in milk-based formulae by high-performance liquid chromatography (HPLC) with refractive index detector was developed and validated. Samples were cleaned up just by protein precipitation before HPLC analysis. Separation was achieved with a Shodex Asahipak NH2P-50 column. The method showed good linearity, sensitivity, precision and recovery, and proved very simple and rapid for routine analysis since separation was completely achieved at 10 min. (c) 2007 Elsevier Inc. All rights reserved.