Article
Cell & Tissue Engineering
Ryo Yokomizo, Yukiko Fujiki, Harue Kishigami, Hiroshi Kishi, Tohru Kiyono, Sanae Nakayama, Haruhiko Sago, Aikou Okamoto, Akihiro Umezawa
Summary: The study successfully established long-term in vitro culture of endometrial epithelial cells using specific cell sources and three-dimensional culture techniques, providing a potential therapeutic option for refractory thin endometrium.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Fawzia Bardag-Gorce, Alissa Diaz, Robert Niihara, Jeremy Stark, Daileen Cortez, Alexander Lee, Richard Hoft, Yutaka Niihara
Summary: This study investigated the expression of ALDHs in rabbit corneas with LSCD and found that grafting with CAOMECS to reconstruct corneal epithelium can reduce the expression levels of ALDH enzymes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Yue Ruan, Subao Jiang, Aytan Musayeva, Norbert Pfeiffer, Adrian Gericke
Summary: This article discusses the regulation of corneal epithelium regeneration by the limbal stem cell reservoir, the consequences of limbal stem cell deficiency caused by various factors, and the therapeutic options available for treating the condition.
Article
Ophthalmology
Davide Borroni, Chiara Bonzano, Rozaliya Hristova, Jose Maria Sanchez Gonzalez, Flavia Pennisi, Aurelio Rocha-Bogas, Carlos Rocha de Lossada
Summary: The new technique of delivering riboflavin using CXL Epi-Pocket method during Corneal Cross-Linking procedure has shown stable results in halting the progression of keratoconus at a 12-month follow-up.
ASIA-PACIFIC JOURNAL OF OPHTHALMOLOGY
(2021)
Review
Immunology
Laurine C. Rijsbergen, Laura L. A. van Dijk, Maarten F. M. Engel, Rory D. de Vries, Rik L. de Swart
Summary: Respiratory tract infections, mainly caused by viruses, are a common cause of morbidity and mortality in humans. In vitro culture models that accurately reflect the human respiratory tract are essential to study virus-host interactions and test novel therapeutic interventions. Recent advances in in vitro models, including primary human airway epithelial cells and human airway organoids, provide new opportunities for research in this field.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Ophthalmology
Promita Bhattacharjee, Mark Ahearne
Summary: Soft lithography was used to create silk fibroin substrates with spiral topographies, which successfully guided human limbal-derived corneal epithelial cells to form a spiral orientation. The study found that the spiral patterns affected cytoskeletal organization, cell spreading, and nuclei shapes, and had significant impacts on cell proliferation, adhesion, chromatin condensation, and actin filament numbers. The spiral pattern also enhanced the expression of markers associated with limbal stem cells.
EXPERIMENTAL EYE RESEARCH
(2022)
Article
Multidisciplinary Sciences
Hyeck-Soo Son, Albert. S. S. Jun, James. W. W. Foster, Wei Wang, Yassine Daoud, Gerd. U. U. Auffarth, Madhuparna Roy
Summary: This study evaluates the efficacy of in situ AAV-mediated gene delivery into the human corneal limbal region via targeted sub-limbal injection technique. The results demonstrate the feasibility and efficiency of the sub-limbal injection technique in specifically targeting cells in the human limbus. This technique may provide a feasible mode of genetic therapy for corneal disorders with an epithelial etiology.
SCIENTIFIC REPORTS
(2022)
Article
Veterinary Sciences
Zebang Xu, Xinxin Xu, Bin Yang, Yuling Mi, Jiakun Wang
Summary: Research has shown that it is feasible to establish organoids from single rumen epithelial cells, which is a novel in vitro system that may reduce the use of experimental animals and can be used to study host and pathogen interaction.
VETERINARY RESEARCH
(2023)
Article
Multidisciplinary Sciences
Tsutomu Imaizumi, Ryuhei Hayashi, Yuji Kudo, Xiaoqin Li, Kaito Yamaguchi, Shun Shibata, Toru Okubo, Tsuyoshi Ishii, Yoichi Honma, Kohji Nishida
Summary: The conditioned medium of human adipose-derived mesenchymal stem cells (hAdMSC-CM) has been found to suppress cytotoxicity and inflammation in human corneal epithelial cells (hCECs), improve barrier dysfunction, and serve as a potential therapeutic agent for dry eye syndrome (DES).
SCIENTIFIC REPORTS
(2023)
Article
Cell Biology
Lu Xiao, Juan Zhu, Zheng Liu, Bangyong Wu, Xiaohua Zhou, Yanxing Wei, Fei Sun, Zhijian Wang, Song Quan, Qi Li, Jun Wang, Liping Huang, Yanlin Ma
Summary: This study discovered that human embryonic stem cells grown in different environments exhibited different gene expression patterns, with significant differential expression of 23 lncRNAs and 15 genes in different culture conditions. Gene ontology analysis showed that these genes were involved in processes such as growth factor stimuli, cell growth, and stem cell maintenance.
Article
Ophthalmology
Marina Bertolin, Mattia Lamon, Elena Franco, Vanessa Barbaro, Stefano Ferrari, Cristina Bovone, Angeli Christy Yu, Mohit Parekh, Diego Ponzin, Massimo Busin
Summary: Endothelial keratoplasty is the preferred surgical treatment for FECD, but a lack of corneal donor tissue in many parts of the world hinders its application. Cell therapy is being investigated as an alternative strategy, with studies showing factors influencing FECD cell culture characteristics and the challenges of using autologous cell therapy in clinical settings. More research is needed to overcome obstacles like low initial cell concentration and endothelial-to-mesenchymal transition for the application of FECD cultures.
EXPERIMENTAL EYE RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yu-Hung Lai, Po-Yen Lee, Chi-Yu Lu, Yu-Ru Liu, Shu-Chi Wang, Ching-Chih Liu, Yo-Chen Chang, Yung-Hsiang Chen, Chia-Cheng Su, Chia-Yang Li, Po-Len Liu
Summary: This study found that TSP1 protects HCE cells from hypoxia-induced damages by regulating exosomal protein expression to promote cell migration, cell-cell adhesion, and extracellular matrix remodeling. TSP1 also improves hypoxia-induced paraptosis in HCE cells by promoting wound healing and remodeling.
Article
Multidisciplinary Sciences
Diya Tang, Masafumi Uematsu, Kohei Harada, Yasser Helmy Mohamed, Mao Kusano, Daisuke Inoue, Takashi Kitaoka
Summary: The research aims to investigate a new preservation device that separates the corneal epithelium and endothelium while preserving the cornea. The results show that corneal epithelium preserved under hypothermic airlift conditions has a greater migration ability compared to preservation under Optisol-GS conditions, and the effect is comparable.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Roman Paduch, Teresa Urbanik-Sypniewska, Jolanta Kutkowska, Tomasz Choragiewicz, Anna Matysik-Wozniak, Sandrine Zweifel, Aleksandra Czarnek-Chudzik, Wojciech Zaluska, Robert Rejdak, Mario Damiano Toro
Summary: The study investigated the biological activity of Ozodrop on human corneal and conjunctival epithelial cells, as well as its antibacterial and antifungal effects. Results showed that Ozodrop exhibited some biological activity but was less effective than standard antibiotics and anti-yeast agents. Additionally, Ozodrop did not show toxicity in the short term and demonstrated good antioxidant and nitric oxide stimulating properties.
Article
Chemistry, Medicinal
Anna Matysik-Wozniak, Waldemar A. Turski, Monika Turska, Roman Paduch, Miroslaw Lancut, Pawel Piwowarczyk, Miroslaw Czuczwar, Robert Rejdak
Summary: The study evaluated the effects of KYNA on corneal and conjunctival cells in vitro and the re-epithelization of corneal erosion in rabbits in vivo. KYNA showed potential in promoting corneal epithelial cell migration, reducing inflammatory cytokine secretion, and accelerating corneal epithelium healing.