Journal
JOURNAL OF FLUORESCENCE
Volume 19, Issue 6, Pages 1017-1023Publisher
SPRINGER/PLENUM PUBLISHERS
DOI: 10.1007/s10895-009-0501-z
Keywords
Squarylium dye; Methemoglobin; Cytochrome c; Lipid peroxidation
Funding
- Science and Technology Center in Ukraine [4534]
Ask authors/readers for more resources
A novel squaraine probe SQ-1 has been found to be appropriate for monitoring the peroxidation processes in membrane systems. Formation of free radicals was triggered by methemoglobin (metHb) or cytochrome c (cyt c) binding to the model lipid membranes composed of zwitterionic lipid phosphatidylcholine (PC) and anionic lipid cardiolipin (CL). Protein association with the lipid vesicles was followed by drastic quenching of SQ-1 fluorescence. The observed spectral changes were suppressed in the presence of free radical scavengers, butylated hydroxytoluene (BHT) and thiourea (TM) suggesting that SQ-1 decolorization can be attributed to its reactions with lipid radicals.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available