Article
Fisheries
Zheng Zhang, Ze-yu Yang, Yin-geng Wang, Mei-jie Liao, Xin-xian Wei, Yong-Xiang Yu, Xiao-Jun Rong, Xia Chen
Summary: The study revealed that S. officinalic could potentially control and prevent VP-induced AHPND in shrimp. Ethanol-extract exhibited stronger bactericidal effects and could inhibit the expression of T6SS-related genes, reducing the pathogenicity of VP to the host. Additionally, a clinical trial confirmed the inhibitory effect of dried garden burnet on Vibrio proliferation in shrimp.
Article
Fisheries
Thao Nguyen, Andrea Alfaro, Bonny Bayot Arroyo, Jenny Antonia Rodriguez Leon, Stanislaus Sonnenholzner
Summary: The study used a metabolomics approach to measure molecular responses of shrimp exposed to Vibrio parahaemolyticus, revealing an increase in metabolic rate, elevated TCA cycle, and gluconeogenesis activities in infected shrimp. This provides important new findings about the pathogenic mechanisms of V. parahaemolyticus invasion in shrimp at the metabolic level.
Article
Gastroenterology & Hepatology
Daniel Tchelet, Kinga Keppel, Eran Bosis, Dor Salomon
Summary: In this study, proteomics was used to identify antibacterial effectors in the secretome of Vibrio parahaemolyticus strains. The results revealed two conserved effectors and other effectors that are only present in subsets of strains, suggesting a core and accessory effector repertoire of the T6SS2 system. A conserved Rhs effector was also discovered to be required for T6SS2 activity.
Article
Gastroenterology & Hepatology
Ce Zhang, Min Liu, Ying Wu, Xixi Li, Chen Zhang, Douglas R. Call, Ming Liu, Zhe Zhao
Summary: In Vibrio parahaemolyticus, the expression of T3SS1 is regulated by a complex quorum sensing phosphorelay circuit involving the hybrid histidine kinase ArcB, sensor kinase LuxQ, and response regulator LuxO. The study shows that LuxQ can directly interact with ArcB under high oxygen conditions, leading to phosphorylation of LuxO and activation of T3SS1 expression. Under hypoxic conditions, ArcB autophosphorylates and phosphorylates ArcA, but ArcA does not participate in regulating T3SS1 expression.
Article
Immunology
Kui Wu, Yongyan Long, Qian Liu, Wei Wang, Guoyin Fan, Hui Long, Yangyun Zheng, Xiansheng Ni, Shengen Chen, Haiying Chen, Shufen Shuai
Summary: This study reveals that the food-borne pathogen Vibrio parahaemolyticus uses quorum sensing signals to regulate the production of its virulence factor T6SS2. CIQS inhibits T6SS2 production through an OpaR-dependent mechanism and also inhibits the adhesion activity of the bacteria to host cells.
MICROBIAL PATHOGENESIS
(2022)
Article
Microbiology
Ramya Kumar, Teng-Chun Tung, Tze Hann Ng, Che-Chih Chang, Yi-Lun Chen, Yi-Min Chen, Shih-Shun Lin, Han-Ching Wang
Summary: The study revealed that AHPND infection can impact lipid metabolism in shrimp stomach, and that the primary bile acid taurocholate is crucial for the virulence of AHPND-causing Vibrio parahaemolyticus.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Cell Biology
Le Tang, Shuqi Dong, Nadia Rasheed, Hao Weng Wu, Ningkun Zhou, Huadong Li, Meilin Wang, Jun Zheng, Jun He, William Chong Hang Chao
Summary: The study reveals that Rhs proteins, an ancient giant protein fold, can act as polymorphic toxins in interbacterial competitions through the ABC complex or T6SS. The research on Vibrio parahaemolyticus shows that RhsP-dependent prey targeting requires T6SS2 and RhsP can self-cleave into fragments, with the toxic C-terminal fragment binding to VgrG2. The alternative conformation of VIR, encapsulated inside the Rhs b barrel structure, triggers RhsP dimerization and plays a significant role in prey targeting.
Article
Fisheries
Dae-Han Kim, L. G. T. G. Rajapaksha, C. W. R. Gunasekara, S. H. M. P. Wimalasena, H. N. K. S. Pathirana, Seok-Ryel Kim, Byoung-Joo Seo, Gang-Joon Heo, Gee-wook Shin
Summary: This study conducted genotype analysis and antibiotic resistance testing on 27 pirAB-harboring Vibrio parahaemolyticus (Vp (AHPND)) isolates from white leg shrimp farms and retail markets affected by acute hepatopancreatic necrosis disease (AHPND) in Korea. The majority of Vp (AHPND) isolates showed resistance to colistin, ampicillin, and streptomycin. MLST analysis revealed new sequence types and suggested a potential common clone origin for AHPND outbreaks in Korea. The detection of Vp (AHPND) in retail shrimp samples indicates potential widespread distribution of the pathogen in Korean shrimp aquaculture and environment.
Article
Immunology
Qian Yang, Peizhuo Zou, Zhi Cao, Qingyao Wang, Songzhe Fu, Guosi Xie, Jie Huang
Summary: The study demonstrated the role of QseC in the global regulation of Vp (AHPND) infection and showed the antivirulence potential of LED209, which is significant for the shrimp aquaculture industry.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Yan Huang, Yue Du, Hong Wang, Dongmei Tan, Airong Su, Xiugui Li, Biao Kan, Lan Lan, Cong Qu, Bo Pang, Yunliang Shi, Mei Lin
Summary: A new serotype of Vibrio parahaemolyticus O10:K4 emerged in Guangxi, China in 2020, potentially becoming dominant and requiring enhanced surveillance and investigation.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Rui Wang, Jinzhou Xiao, Qingyao Wang, Wenyu Zhao, Xinyue Liu, Ying Liu, Songzhe Fu
Summary: In this study, genomic analysis of a T6SS-harboring plasmid in Vibrio parahaemolyticus strain VP157 was conducted, and coculture assays were used to verify its antibacterial function. The results showed that acquisition of T6SS fostered the fitness of V. parahaemolyticus in complex environments.
CANADIAN JOURNAL OF MICROBIOLOGY
(2022)
Article
Fisheries
K. G. Aguilar-Rendon, S. A. Soto-Rodriguez, B. Gomez-Gil, R. Lozano-Olvera, B. Yanez-Rivera
Summary: This study investigated the impact of pathogenic strains causing acute hepatopancreatic necrosis disease (AHPND) on the water microbiome. The results showed that the virulence of the strains and the duration of the experiment could alter the water microbiome, particularly leading to a significant reduction in the reads assigned to Neptuniibacter spp. This depletion suggests a possible dual function of the type VI secretion system 1 (T6SS1) in the pathogenic strains, killing surrounding bacteria and damaging the hepatopancreas.
Article
Fisheries
Nitin Chandra Teja Dadi, Pattanan Yatip, Kulatara Krataitong, Panida Unagul, Satinee Suetrong, Sita Preedanon, Anupong Klaysuban, Tanuwong Sangtiean, Jariya Sakayaroj, Chumporn Soowannayan
Summary: Endophytic fungi, particularly those from Thai mangrove forests, have been identified as potential sources for inhibitors of Vibrio growth. One specific fungus (MCR433) showed significant protection against AHPND in shrimp feeding trials, indicating its potential as a feed additive alternative to antibiotics. Additional research is needed on the compounds produced by these fungi for further development and application.
Article
Microbiology
Haipeng Guo, Xuezhi Fu, Jikun He, Ruoyu Wang, Mengchen Yan, Jing Wang, Pengsheng Dong, Lei Huang, Demin Zhang
Summary: This study reveals the important role of gut bacterial community induced by a biofloc system (BFS) in protecting shrimp from bacterial pathogens. The study also provides a micro-ecological regulation strategy to improve aquatic animal diseases.
Article
Microbiology
Alejandro Castellanos, Leda Restrepo, Leandro Bajana, Irma Betancourt, Bonny Bayot, Alejandro Reyes
Summary: AHPND is a disease causing significant losses in the shrimp farming industry, with global losses exceeding $2.6 billion. The most common etiological agent is V. parahaemolyticus strains carrying the PirAB(vp) toxin. By analyzing South American AHPND-causing V. parahaemolyticus isolates at the genomic level, it was found that they have high similarity but do not cluster with other Mexican strains, suggesting different genetic backgrounds and possible acquisition of the pVA1-type plasmid through horizontal gene transfer at different times.
MICROBIOLOGY SPECTRUM
(2023)
