Article
Agriculture, Dairy & Animal Science
Mohamed Shawky, Engy Taha, Basem Ahmed, Mahmoud Aly Mahmoud, Mohamed Abdelaziz, Mohamed Faisal, Ausama Yousif
Summary: This study investigated the outbreak of lymphocystis disease in juvenile gilthead seabreams imported to Egypt from Europe, identifying the causative agent as lymphocystis disease virus genotype I, with genetic similarity to strains found in Northern European countries. These findings contribute to the understanding of the ecology of lymphocystis disease and its viral genotypes in the region.
Article
Fisheries
L. Vazquez-Salgado, J. G. Olveira, C. P. Dopazo, I. Bandin
Summary: This study assessed the susceptibility of gilthead seabream and Senegalese sole to two reassortant NNV strains and the possibility of interspecies transmission by cohabiting infected and naive individuals. The results showed that both NNV isolates caused moderate mortality rates and replicated in both fish species. Interspecies transmission was also observed, with infective NNV particles recovered from naive cohabitants.
Article
Fisheries
Reshma R. Nair, K. Riji John, Rahul Krishnan, Ashwini Gopi, Muhammed P. Safeena
Summary: A sudden disease outbreak was reported in the Asian seabass cultured in open brackish water cages along the west coast of Kerala, India. The disease was identified as Lymphocystis disease virus (LCDV) belonging to the genus Lymphocystivirus within the family Iridoviridae. Phylogenetic analysis revealed a high similarity between the isolated strain and LCDV strain in painted glass fish from Japan.
AQUACULTURE INTERNATIONAL
(2023)
Article
Fisheries
Lucia Vazquez-Salgado, Jose G. Olveira, Carlos P. Dopazo, Isabel Bandin
Summary: Intensive fish farming at high densities has negative effects on fish welfare, with NNV-infected Senegalese sole reared at high densities showing earlier mortality onset and higher viral load.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Lenaig Louboutin, Nolwenn M. Dheilly, Joelle Cabon, Sara Picon Camacho, Aurelie Leroux, Pierrick Lucas, Alain Le Breton, Yannick Blanchard, Thierry Morin
Summary: The present study investigated an unusual mortality among 50-day post-hatching gilthead seabream larvae in a hatchery, revealing the presence of a novel Potamipivirus species associated with focal degeneration in the intestinal mucosa. The viral agent, with a typical picornavirus genome organization, showed a high sequence similarity to Eel Picornavirus-1. Further studies are needed to determine the etiological role and prevalence of this virus in natural and aquaculture-reared populations of gilthead seabream.
JOURNAL OF FISH DISEASES
(2022)
Article
Biochemistry & Molecular Biology
Jennifer M. Podgorski, Krista Freeman, Sophia Gosselin, Alexis Huet, James F. Conway, Mary Bird, John Grecco, Shreya Patel, Deborah Jacobs-Sera, Graham Hatfull, Johann Peter Gogarten, Janne Ravantti, Simon J. White
Summary: Many double-stranded DNA viruses, including tailed bacteriophages and herpesviruses, use the HK97-fold in their major capsid protein. The evolution of capsid structural stability within the actinobacteriophages has been investigated using cryo-EM structure determination, structural comparisons, phylogenetic analyses, and AlphaFold predictions. The actinobacteriophage major capsid proteins can be classified into 15 groups based on their HK97-fold.
Article
Biochemistry & Molecular Biology
Alexander Christiansen, Marie Weiel, Andreas Winkler, Alexander Schug, Jochen Reinstein
Summary: It is largely unknown how individual capsid proteins form stable oligomeric units (capsomers) and adapt structurally for further assembly. Using HDX mass spectrometry, researchers discovered differences in conformational flexibility between functional elements of the MCP trimer, with the N-terminal arm and clasp regions showing above average deuterium incorporation. Detachment of the N-terminal arm is crucial for capsomer dissociation and leads to irreversible structural rearrangements.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Mart Krupovic, Kira S. Makarova, Eugene V. Koonin
Summary: The double jelly-roll major capsid protein (DJR-MCP) is not a unique innovation of viruses, but has a common ancestry with a family of cellular proteins involved in carbohydrate metabolism. The findings suggest that bamfordviruses evolved from nonviral replicators by recruiting host proteins for capsid formation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Fisheries
D. Montero, F. J. Moyano, M. Carvalho, S. Sarih, R. Fontanillas, M. J. Zamorano, S. Torrecillas
Summary: The promotion of sustainability and efficiency in aquaculture production requires novel nutritional strategies with sustainable raw materials and successful breeding programs. Genetic variability in fish species allows for adaptation to plant-based diets and the utilization of emergent ingredients.
Article
Infectious Diseases
Qiao Tang, Zhiwei Chen, Hu Li, Li Zhang, Mingli Peng, Yi Zeng, Xiaoqing Liu, Zubi Liu, Peng Hu
Summary: This study investigated the distribution of HCV genotypes in Chinese mainland and performed phylogenetic analysis to provide an up-to-date understanding of the molecular epidemiology of genotypes.
INFECTIOUS DISEASES OF POVERTY
(2023)
Article
Fisheries
Quan Wang, Xia Liang, Yunshang Ning, Shijia Liu, Zengjian Liang, Zemiao Zhang, Yingjing Chen, Jinqiao Cao, Fubao Wang, Lingfeng Lan, Guangzhao Cheng, Youhua Huang, Yan Huang, Qiwei Qin, Sheng Zhou
Summary: This study successfully constructed the CotC-LMBV recombinant B. subtilis spores and evaluated its protective efficacy and immune responses. The results showed that largemouth bass orally vaccinated with CotC-LMBV spores had a survival rate of 53.3% and a relative percent survival of 45.0% compared to the control group. The specific IgM level in serum and the expression of immune-related genes in the spleen were also significantly increased in the vaccinated group.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Medicine, General & Internal
Zhongping Liu, Yafei Zhang, Mengyuan Xu, Xu Li, Zhenhua Zhang
Summary: This study conducted a meta-analysis of 309 datasets from 110 countries to provide a more detailed and accurate description of HBV genotype and subgenotype distribution globally. The findings suggest that the differences in genotype distribution may be related to ethnicity and human migration in different regions.
Article
Virology
Wen-Jing Zhang, Ren-Qi Wang, Lin-Tao Li, Wen Fu, Huan-Chun Chen, Zheng-Fei Liu
Summary: The study reveals that Hsp90 plays a role in pseudorabies virus (PRV) replication by stabilizing the major capsid protein VP5, assisting in virion assembly.
Article
Virology
Xu Wang, Jianjun Hu, Fanyan Meng, Yiheng Cao, Zijie Wang, Qianyi Zhang, Qian Zhang, Xingxing Zhang, Mengli Han, Tongzhong Wu, Fagang Zhong, Xin Huang
Summary: This study isolated and sequenced two genotypes (B and C) of bovine parainfluenza virus 3 (BPIV3) from Chinese cattle herds. The genomes of the isolates had different lengths and multiple amino acid changes in major antigenic proteins. These findings contribute to tracing the origin of BPIV3, understanding genotype differences, and supporting vaccine development.
Article
Fisheries
Akapon Vaniksampanna, Orapan Manajit, Saengchan Senapin, Suphattra Kamsamarn, Pradit Wangman, Siwapon Longyant, Parin Chaivisuthangkura
Summary: In this study, specific monoclonal antibodies (MAbs) against the major capsid protein (MCP) of Infectious spleen and kidney necrosis virus (ISKNV) were generated. These MAbs showed high sensitivity and specificity in detecting the virus in fish and could potentially be used for the detection of other related viruses.
Article
Immunology
Juan Gemez-Mata, Daniel Alvarez-Torres, Esther Garcia-Rosado, M. Carmen Alonso, Julia Bejar
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2020)
Article
Gastroenterology & Hepatology
R. Kalla, A. T. Adams, N. T. Ventham, N. A. Kennedy, R. White, C. Clarke, A. Ivens, D. Bergemalm, S. Vatn, B. Lopez-Jimena, P. Ricanek, M. H. Vatn, J. Soderholm, F. Gomollon, J. K. Nowak, J. Jahnsen, J. Halfvarson, S. McTaggart, G. T. Ho, A. Buck, J. Satsangi
JOURNAL OF CROHNS & COLITIS
(2020)
Article
Genetics & Heredity
Juan Gemez-Mata, Alejandro M. Labella, Isabel Bandin, Juan J. Borrego, Esther Garcia-Rosado
Summary: The transcriptomic response of Senegalese sole to two betanodaviruses with different virulence levels was studied, revealing that the highly virulent strain induced a stronger immune response but also increased disease severity, while the moderately virulent strain did not. Furthermore, specific genes showed significantly increased expression levels after infection with the highly virulent virus, while immune response was lacking after infection with the mutant strain.
Article
Infectious Diseases
Sebastian Hin, Benjamin Lopez-Jimena, Mohammed Bakheit, Vanessa Klein, Seamus Stack, Cheikh Fall, Amadou Sall, Khalid Enan, Mohamed Mustafa, Liz Gillies, Viorel Rusu, Sven Goethel, Nils Paust, Roland Zengerle, Sieghard Frischmann, Manfred Weidmann, Konstantinos Mitsakakis
Summary: The study presents a LabDisk platform for differential diagnosis of acute fever caused by various pathogens, demonstrating promising results for detecting tropical diseases and precise identification of the cause of acute fever.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Environmental Sciences
Alejandro Labella, Rosana Molero, Rocio Leiva-Rebollo, Rocio Perez-Recuerda, Juan J. Borrego
Summary: This study analyzed the bacterial composition and antibiotic resistance of a drinking-water RO system, identifying Gammaproteobacteria, Firmicutes, and Betaproteobacteria as the major bacterial groups. Among the strains, 37.2% showed resistance to three or more classes of antibiotics.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Agriculture, Dairy & Animal Science
Rocio Leiva-Rebollo, Dolores Castro, Patricia Moreno, Juan J. Borrego, Alejandro M. Labella
Summary: The study aimed to develop a DNA vaccine for the lymphocystis disease in gilthead seabream. The results showed that the vaccine triggered an immune response and produced specific anti-viral antibodies, providing protection against the disease.
Article
Microbiology
Juan Gemez-Mata, Sandra Souto, Isabel Bandin, Maria del Carmen Alonso, Juan Jose Borrego, Alejandro Manuel Labella, Esther Garcia-Rosado
Summary: The study revealed significant differences in immune responses triggered by different recombinants in infected sole, with the RNA2 mutant causing the most prominent immune response changes, especially the exclusive and high upregulation of genes related to the complement system. Infection with RNA1 mutants also led to reduced mortality and delayed replication compared to wt160, potentially causing the observed lag in immune response.
Article
Fisheries
Patricia Moreno, Rocio Leiva-Rebollo, Esther Garcia-Rosado, Julia Bejar, M. Carmen Alonso
Summary: This study investigates the impact of sea bass ISG15 on RGNNV replication and confirms its cytokine-like activity and immunomodulatory function.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Microbiology
Sergio Ortega-del Campo, Luis Diaz-Martinez, Patricia Moreno, Esther Garcia-Rosado, M. Carmen Alonso, Julia Bejar, Ana Grande-Perez
Summary: NNV is a neurotropic virus that causes viral nervous necrosis disease in fish. It has a bisegmented (+) ssRNA genome and the variability of its genome may be associated with its virulence.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Fisheries
Patricia Moreno, Daniel Alvarez-Torres, M. Carmen Balebona, Marta Dominguez-Maqueda, M. Angel Morinigo, Julia Bejar, M. Carmen Alonso, Esther Garcia-Rosado
Summary: Viral encephalopathy and retinopathy caused by nervous necrosis virus (NNV) is a significant infectious disease in farmed fish. This study investigates the antiviral activity of a probiotic, SpPdp11, against a specific NNV species, RGNNV, in vitro and in vivo. The results demonstrate that SpPdp11 extracts can inhibit RGNNV multiplication and induce an immune response, making it a potential prophylactic tool against RGNNV in aquaculture.
Article
Fisheries
Patricia Moreno, Juan Gemez-Mata, Esther Garcia-Rosado, Julia Bejar, Alejandro M. Labella, Sandra Souto, M. Carmen Alonso
FISH & SHELLFISH IMMUNOLOGY
(2020)
