Article
Parasitology
Caixia Dong, Ziye Liu, Xiaolin Li
Summary: Our Giardia chemiluminescence assay (GCA) detected Giardia antigens in a dose-dependent manner with a detection limit of 0.46 ng/mL and a signal-to-baseline ratio of 475. In a study using 30 clinic collected canine stool samples, the GCA showed a sensitivity of 93.8% and a specificity of 92.9% compared to the standard Giardia II ELISA. Additionally, the GCA displayed higher signal-to-baseline ratios and equal or higher antigen titers compared to a colorimetric ELISA in a study of 16 Giardia positive samples.
VETERINARY PARASITOLOGY
(2023)
Article
Microbiology
Dong Chan Moon, Ji-Hyun Choi, Naila Boby, Su-Jeong Kim, Hyun-Ju Song, Ho-Sung Park, Min-Chan Gil, Soon-Seek Yoon, Suk-Kyung Lim
Summary: This study investigated the prevalence of pathogenic bacteria associated with different site infections in cats in the Republic of Korea. Results showed that Escherichia coli was the most prevalent species in diarrheal stool and urine, while Staphylococcus felis and Pasteurella multocida were most prevalent in the skin and respiratory tract, respectively. There was no significant difference in bacterial distribution among different age groups in all samples.
Article
Veterinary Sciences
Fabienne Sprissler, Prapaporn Jongwattanapisan, Supol Luengyosluechakul, Rosama Pusoonthornthum, Sven Reese, Michele Bergmann, Katrin Hartmann
Summary: This study investigated the prevalence of FIV and FeLV infection in healthy outdoor cats in North, Northeast and Central Thailand and evaluated the associated risk factors. The results showed that the prevalence of FIV and FeLV infections was lower than expected in these regions, and there were no significant risk factors for FeLV infection. However, the risk of FIV infection increased with age.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Spectroscopy
Sritam Biswas, Yengkhom Damayanti Devi, Dipjyoti Sarma, Diganta Hatiboruah, Nabadweep Chamuah, Nima D. Namsa, Pabitra Nath
Summary: This study presents a low-cost and sensitive SERS substrate for detecting rotavirus in clinical stool samples. The substrate is fabricated by drop-casting silver nanoparticles on printing-grade paper. It is confirmed that the substrate can enhance the characteristic Raman peaks of rotavirus particles in solution. The proposed substrate has the potential to be widely used in research and clinical laboratories.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Microbiology
Dong-Chan Moon, Ji-Hyun Choi, Naila Boby, Hee-Young Kang, Su-Jeong Kim, Hyun-Ju Song, Ho-Sung Park, Min-Chan Gil, Soon-Seek Yoon, Suk-Kyung Lim
Summary: The prevalence and significance of bacterial infections in companion animals, specifically dogs, in Korea were investigated in this nationwide surveillance study. The results showed the distribution of bacterial species and prevalence rates in different samples obtained from veterinary hospital-visited dogs, providing valuable information for assessing bacterial infections and guiding therapeutic decisions.
Article
Microbiology
Romy Razakandrainibe, Celia Merat, Nathalie Kapel, Marc Sautour, Karine Guyot, Gilles Gargala, Jean-Jacques Ballet, Patrice Le Pape, Frederic Dalle, Loic Favennec
Summary: The coproantigen ELISA test shows high sensitivity and specificity for routine diagnosis of Cryptosporidium spp., indicating its potential usefulness for large-scale epidemiological studies of cryptosporidiosis.
Article
Microbiology
Andreea Tirziu, Viorel Herman, Kalman Imre, Diana Maria Degi, Marius Boldea, Vlad Florin, Timea Andrea Bochis, Marcu Adela, Janos Degi
Summary: This study investigates the presence of Chlamydia spp. in stray cats in Timisoara Municipality, Western Romania, and finds that a high percentage of cats are infected, with a higher detection rate in asymptomatic cats. This highlights the potential zoonotic risk of this pathogen and emphasizes the role of urban stray cats as sources of feline chlamydiosis.
Article
Parasitology
Christian M. Leutenegger, Cecilia E. Lozoya, Jeffrey Tereski, Jan Andrews, Kelly D. Mitchell, Cathy Meeks, Jennifer L. Willcox, Gregory Freeman, Holly L. Richmond, Christian Savard, Michelle D. Evason
Summary: This study compared the performance of real-time PCR (qPCR) and zinc sulfate centrifugal fecal flotation microscopy (ZCF) for fecal parasite screening. The results showed that qPCR had a significantly higher detection frequency than ZCF and could detect parasite markers that were undetected by ZCF.
PARASITES & VECTORS
(2023)
Article
Veterinary Sciences
Ivo Sirakov, Nikolina Rusenova, Anton Rusenov, Raina Gergova, Tanya Strateva
Summary: The aim of this study was to verify whether the human DR-ELISA for the detection of anti-SARS-CoV-2 antibodies can be applied in cats and to assess the risk factors that determine the spread of the virus among the cat population in Bulgaria. The study included 92 serum samples collected from 68 domestic and 24 stray cats, and the results showed a high seroprevalence in both stray cats and domestic cats. Cats under 7 years and stray cats had a higher risk, and the risk was highest for cats in villages.
VETERINARY SCIENCES
(2023)
Article
Parasitology
Thais Nascimento de Andrade Oliveira Cruz, Luiz Ricardo Goncalves, Maria Eduarda Chiaradia Furquim, Marcos Rogerio Andre, Alexandre Diaz Munhoz, Renata Santiago Alberto Carlos, Fabiana Lessa Silva
Summary: This study investigated the occurrence of Bartonella spp. in blood and claw samples from domestic cats in northeastern Brazil, and found that 20.7% of the blood samples and 23.9% of the claw samples were positive. Animals positive for Bartonella spp. showed higher platelet values in the blood samples. Young and unsterilized cats with outdoor access were more prone to infection by Bartonella spp.
Article
Multidisciplinary Sciences
Babak Pakbin, Leila Zolghadr, Shahnaz Rafiei, Wolfram Manuel Brueck, Thomas B. Brueck
Summary: The study developed a sensitive and specific method using FTIR, PCA, and HCA assays to differentiate four species of Shigella isolates from stool samples. The FTIR method showed distinct spectra for each species, with high specificity and sensitivity for identifying and differentiating all species in the samples.
SCIENTIFIC REPORTS
(2022)
Article
Veterinary Sciences
Daseul Yeo, Md. Iqbal Hossain, Soontag Jung, Zhaoqi Wang, Yeeun Seo, Seoyoung Woo, Sunho Park, Dong Joo Seo, Min Suk Rhee, Changsun Choi
Summary: This study investigated the prevalence of human enteric viruses in porcine stools and found that porcine stools frequently contain human enteric viruses, with some genetic similarity to human enteric viruses.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Sunil Tulshiram Hajare, Yeinewub Chekol, Nitin Mahendra Chauhan
Summary: Giardiasis, a protozoan disease caused by the parasite Giardia lamblia, has a high global infection rate. This study aimed to assess the prevalence and associated risk factors of G. lamblia infection among elementary school children in Loka Abaya town, Ethiopia. The study found that factors such as rural school location, age group of 6-9 years, consuming tap water, hand washing habits after defecation, and the use of soap and water for hand washing were associated with the parasite infection. Therefore, scaling up access to safe water, toilets, education, and health facilities is crucial in addressing this issue.
Article
Agriculture, Dairy & Animal Science
Clarisse D'Aout, Samantha S. Taylor, Stefania Gelendi, Carl Atkinson, Pieter Defauw
Summary: This study investigated the prevalence and antimicrobial resistance of bacteria in feline urine samples in the UK. It identified E. coli, other Enterobacterales, Enterococcus species, and Staphylococcus species as the most common bacteria, with significant resistance to commonly used antibiotics observed.
Review
Immunology
Farzad Mahdavi, Morteza Shams, Alireza Sadrebazzaz, Laya Shamsi, Mostafa Omidian, Ali Asghari, Soheil Hassanipour, Amir Masoud Salemi
Summary: This review explores the prevalence and risk factors of giardiasis in HIV/AIDS patients, indicating that diarrhea is a significant risk factor and patients without ART treatment are more susceptible to G. duodenalis infection.
MICROBIAL PATHOGENESIS
(2021)
Article
Veterinary Sciences
Mirabela Oana Dumitrache, Adriana Gyorke, Gianluca D'Amico, Viorica Mircean
Summary: This case report describes a clinical case of a 14-month-old cat with dermatitis linked to L. gibbus, which is the first report of L. gibbus-related dermatitis in cats. L. gibbus infestation should be considered as a possible differential diagnosis of pruritic skin conditions in cats.
BMC VETERINARY RESEARCH
(2021)
Article
Parasitology
Maria E. Nedisan, Adriana Gyorke, Cristina L. Stefanut, Zsuzsa Kalmar, Zsuzsa Friss, Radu Blaga, Amandine Blaizot, Andra Toma-Naic, Viorica Mircean, Gereon Schares, Olgica Djurkovic-Djakovic, Ivana Klun, Isabelle Villena, Vasile Cozma
Summary: The study found that chickens infected with Toxoplasma gondii did not show clinical signs of infection, but infection could be detected through serum antibody testing. Artemisia annua reduced parasite replication, while diclazuril was not effective in preventing infection. Further studies with quantitative analysis methods are needed to evaluate the efficacy of diclazuril and Artemisia annua for preventing T. gondii infection in chickens.
PARASITOLOGY RESEARCH
(2021)
Article
Microbiology
Aurora L. Ursache, Adriana Gyorke, Viorica Mircean, Mirabela O. Dumitrache, Andrei Rizvan Codea, Vasile Cozma
Summary: The study found that cats with intestinal parasites and digestive clinical signs had a significantly higher prevalence than clinically healthy cats, and lack of deworming, outdoor access, digestive symptoms, and young age are risk factors for Toxocara cati infection.
Article
Microbiology
Zsolt Boros, Mihai Horia Baies, Dan Cristian Vodnar, Calin Mircea Gherman, Silvia-Diana Borsan, Anamaria Cozma-Petrut, Menelaos Lefkaditis, Adriana Gyorke, Vasile Cozma
Summary: This study evaluated the antiparasitic effect of Lactobacillus casei ATCC 393 and L. paracasei CNCM on mice infected with Trichinella britovi. The results showed that treatment with these probiotics significantly reduced the number of adult parasites in the intestines of the mice. Female mice had better response to L. paracasei CNCM treatment compared to males.
Article
Parasitology
Diana Ancuta Barburas, Vasile Cozma, Angela Monica Ionica, Ibrahim Abbas, Remus Barburas, Viorica Mircean, Gianluca D'Amico, Jitender P. Dubey, Adriana Gyorke
Summary: The study revealed extensive parasitic infections in buffalo calves from northwestern Romania, highlighting the necessity of prophylactic treatments for T. vitulorum and E. bareillyi.
FOLIA PARASITOLOGICA
(2022)
Article
Microbiology
Gianluca D'Amico, Angela Monica Ionica, Adriana Gyorke, Mirabela Oana Dumitrache
Summary: This study is the first to assess the prevalence of tick-borne pathogens in dogs in the Republic of Moldova. The most prevalent pathogens were Hepatozoon canis, followed by Babesia canis, Anaplasma phagocytophilum, and Bartonella spp. Dogs from veterinary clinics and Chisinau had higher infection rates of certain pathogens compared to those from shelters and Cahul.
Article
Microbiology
Mihai-Horia Baies, Calin Gherman, Zsolt Boros, Diana Olah, Ana-Maria Vlase, Anamaria Cozma-Petrut, Adriana Gyorke, Doina Miere, Laurian Vlase, Gianina Crisan, Marina Spinu, Vasile Cozma
Summary: This study comparatively evaluated the in vitro antiparasitic effects of Allium sativum L., Artemisia absinthium L., Cucurbita pepo L., Coriandrum sativum L., Satureja hortensis L. and Calendula officinalis L. against Ascaris suum egg hatching and larval development, and found that garlic and wormwood extracts exhibited the strongest anthelminthic activity.
Article
Microbiology
Mihai-Horia Baies, Zsolt Boros, Calin Mircea Gherman, Marina Spinu, Attila Mathe, Stefan Pataky, Menelaos Lefkaditis, Vasile Cozma
Summary: The study investigated the parasitic profile of swine raised on two free-range farms in Transylvania, revealing significant differences in prevalence and intensity of infections between age groups, seasons, and farms. Further research is needed to better understand the epidemiology of these parasitic infections in swine from Transylvania.
Article
Microbiology
Mihai-Horia Baies, Adriana Gyorke, Vlad-Dan Cotutiu, Zsolt Boros, Anamaria Cozma-Petrut, Lorena Filip, Laurian Vlase, Ana-Maria Vlase, Gianina Crisan, Marina Spinu, Vasile Cozma
Summary: This study aimed to evaluate the in vitro anticoccidial effects of various plant extracts against Eimeria suis and Eimeria debliecki oocysts. The results showed that the marigold extract had the best effects on oocyst sporulation and destruction. This study provides a preliminary contribution to the development of natural disinfectants for eliminating Eimeria oocysts in swine contamination.
Article
Microbiology
Mihai-Horia Baies, Vlad-Dan Cotutiu, Marina Spinu, Attila Mathe, Anamaria Cozma-Petrut, Doina Miere, Sorana D. Bolboaca, Vasile Cozma
Summary: Parasitic diseases cause significant losses in swine reproduction and productivity, impacting efficient and profitable livestock management. Phytotherapeutic remedies, such as Cucurbita pepo L. and Coriandrum sativum L., have gained popularity due to their bioavailability, decreased toxicity, non-polluting nature, and antiparasitic effects. This study evaluated the antiparasitic potential of these plants against protozoa and nematodes in swine, finding that a specific dosage demonstrated pronounced anthelmintic and antiprotozoal effects. Further research is needed to determine the optimal dosage for maximum antiparasitic effectiveness. This study provides the first Romanian report on the in vivo antiparasitic activity of these plants on digestive parasites in swine.
Review
Parasitology
Calin Mircea Gherman, Zsolt Boros, Mihai-Horia Baies, Anamaria Cozma-Petrut, Vasile Cozma
Summary: This study assessed the status of Trichinella species in wild animals in Romania over the past 30 years. The findings revealed that different wild animal species were infected with different Trichinella species, and the prevalence rates varied. Trichinella infection remains prevalent in Romania.
FOOD AND WATERBORNE PARASITOLOGY
(2022)
Article
Parasitology
Z. Boros, A. M. Ionica, G. Deak, A. D. Mihalca, G. Chisamera, I. C. Constantinescu, C. Adam, C. M. Gherman, V Cozma
Summary: The European polecat is declining in Romania and often lives near human settlements, feeding on small animals. A study confirmed the occurrence of Trichinela spp. infection in European polecats from Romania, with Trichinella spiralis and T. britovi being the identified parasite species, including a case of co-infection.
