Article
Pharmacology & Pharmacy
Francesca Cavaliere, Pietro Cozzini
Summary: This study used molecular dynamic simulations to reveal the structural effects of R-bicalutamide on the stability of AR homodimers. The results showed that the binding of R-bicalutamide increased the instability of the homodimer, preventing AR migration to the nucleus and transcriptional activity. The W741L mutation resulted in behavior similar to an agonistic system, which may explain the effect of this mutation on drug resistance.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2022)
Article
Genetics & Heredity
Haoran Zhang, Yijie Gao, Guiyu Wang, Qingqing Xin, Xue Tian, Limin Wu, Xi Shi, Wenge Ma, Huifen Liu, Hongxia Jiang, Qisheng Wu, Xuejun Li, Xiao Ma
Summary: This study cloned the full-length cDNA of Ps-Sirt1 in Pelodiscus sinensis and found that it is highly expressed in the ovary and has a significant impact on follicle development. Activated and inactivated Sirt1 affect the expression levels of multiple key genes. Therefore, Sirt1 may play an important role in ovarian follicle development in P. sinensis.
Article
Biochemistry & Molecular Biology
Mengping Long, Chong You, Qianqian Song, Lina X. J. Hu, Zhaorong Guo, Qian Yao, Wei Hou, Wei Sun, Baosheng Liang, Xiaohua Zhou, Yiqiang Liu, Taobo Hu
Summary: This study analyzed the expression of androgen receptor (AR) in both estrogen receptor (ER)-positive and ER-negative breast cancer, and its association with clinicopathological and molecular features. The results showed that AR negativity was associated with different characteristics in ER-positive and ER-negative breast cancer. AR-positive breast cancer had better clinicopathological features, especially in the ER-negative subtype. These findings suggest a distinctive role of AR in ER-negative breast cancer compared to ER-positive breast cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medical Laboratory Technology
Dan Zhou, Hua Xu, Xiaorong Shen, Ruihuan Gu, Ying Chen, Guowu Chen, Pan Li, Huijuan Shi, Xiaoxi Sun, Aijie Xin
Summary: This study reported a novel mutation in the AR gene causing androgen insensitivity syndrome, which resulted in decreased mRNA and protein expression as well as reduced protein stability. In addition, the mutant AR failed to bind to ligand and translocate from cytoplasm to nucleus.
CLINICA CHIMICA ACTA
(2022)
Article
Engineering, Environmental
Phum Tachachartvanich, Ettayapuram Ramaprasad Azhagiya Singam, Kathleen A. Durkin, J. David Furlow, Martyn T. Smith, Michele A. La Merrill
Summary: Research findings indicate that emerging per- and poly-fluoroalkyl substances (PFASs) may have adverse effects on the human endocrine system and exhibit competitive antiandrogenic effects.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Oncology
Miki Naito, Kenichiro Ikeda, Shunya Aoyama, Mayu Kanamoto, Yasuyuki Akasaka, Yuri Kido, Mikako Nakanishi, Machi Kanna, Takeshi Yamamotoya, Akio Matsubara, Nobuyuki Hinata, Tomoichiro Asano, Yusuke Nakatsu
Summary: Par14, an isoform of Pin1 homologous to prolyl isomerases, is shown to be overexpressed in prostate cancer (PCa) and plays a critical role in PCa progression. It enhances the growth of androgen-sensitive LNCap cells and regulates the expression of androgen response genes through its association with AR and modulation of p53 localization. These findings suggest Par14 as a potential therapeutic target for PCa.
Article
Agriculture, Dairy & Animal Science
Rujuan Zhang, Mingshu Wang, Anchun Cheng, Qiao Yang, Ying Wu, Renyong Jia, Shun Chen, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Shaqiu Zhang, Juan Huang, Xumin Ou, Sai Mao, Qun Gao, Yanling Yu, Ling Zhang, Yunya Liu, Bin Tian, Leichang Pan
Summary: In this study, the cDNA sequence of duck CD40 gene was cloned and its role in antiviral innate immune response was confirmed, indicating the importance of duck CD40 molecule in the immune responsiveness against certain viruses. These findings provide valuable insights for a better understanding of the avian immune system.
Article
Multidisciplinary Sciences
Chizuru Ito, Tsukasa Makino, Tohru Mutoh, Masahide Kikkawa, Kiyotaka Toshimori
Summary: Normal sperm flagellar shape and movement are crucial for fertilization. The proteins ODF4, AK1, and AK2 play important roles in the morphology and function of sperm flagella. Deletion of ODF4 leads to abnormal flagellar shape and movement, resulting in male infertility. This study demonstrates the relationship between ODF4, AK1, and AK2 in regulating flagellar structure and function.
SCIENTIFIC REPORTS
(2023)
Article
Fisheries
Ling Pan, Jianfei Shen, Hongyu Liu, Beiping Tan, Xiaohui Dong, Qihui Yang, Shuyan Chi, Shuang Zhang
Summary: The study cloned and identified the glucose transporter 2 (ToGLUT2) of pompano and investigated the effects of exogenous enzyme supplementation in high carbohydrate diets on ToGLUT2 gene expression. The results showed that ToGLUT2 gene was ubiquitously expressed among tissues tested, with highest expression in the liver. Additionally, the addition of carbohydrase improved the activity of digestive enzymes and had impacts on growth performance, glucose metabolism, and insulin concentration of pompano.
AQUACULTURE REPORTS
(2022)
Review
Biochemistry & Molecular Biology
Tae Jin Kim, Young Hwa Lee, Kyo Chul Koo
Summary: The androgen receptor (AR) plays a crucial role in the development and progression of prostate cancer (PCa), and treatment for hormone-sensitive prostate cancer (HSPC) relies heavily on androgen deprivation therapy (ADT). Despite most patients progressing to castration-resistant prostate cancer (CRPC), studies suggest that manipulating alternative molecular pathways can help improve current treatments and develop novel therapies for CRPC management.
Article
Biochemistry & Molecular Biology
Hui-Xia Yu, Yang Li, Wei-Jia Song, Hui Wang, Hao-Lin Mo, Qiao Liu, Xin-Miao Zhang, Ze-Bin Jiang, Li-Xin Wang
Summary: This study characterized the rainbow trout MC3R and found its close relation to mammalian MC3R. The transcripts of rtMC3R were highly expressed in the brain and muscle. rtMC3R can be activated by agonists and modulate multiple signal pathways, while inhibiting the NF-kappa B signaling pathway.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Microbiology
Somayyeh Rahimnahal, Amir Meimandipour, Jamal Fayazi, Ali Asghar Karkhane, Mehdi Shamsara, Mohammadtaghi Beigi Nassiri, Hamed Mirzaei, Michael R. R. Hamblin, Hossein Tarrahimofrad, Hamid Bakherad, Javad Zamani, Yahya Mohammadi
Summary: The keratinase KRLr1 secreted by Bacillus licheniformis has potential industrial applications. The recombinant KRLr1 was successfully expressed, purified, and found to have optimum activity at pH 6 and 37 degrees C. It exhibited increased activity in the presence of Ca2+ and Mg2+. Digestion of feathers with the recombinant enzyme showed higher amounts of cysteine, phenylalanine, tyrosine, and lysine compared to other amino acids. Molecular dynamics simulation indicated strong interaction between KRLr1 and chicken feather keratin 4 (FK4).
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Dermatology
Yicen Yan, Jie Tian, Yang Wang, Yurong Li, Chong Zhang, Shenxi Zhang, Pingping Lin, Rui Peng, Chunxia Zhao, Le Zhuang, Binbin Lai, Liang Zhou, Guohong Zhang, Hang Li
Summary: Multiomic studies, including RNA sequencing, were conducted to explore the connection between gene expression profile of the skin and dermatoses-predisposed sites. The results showed that different regions of the skin have specific physiological characteristics and differential gene expression, which are associated with the development of adjacent tissues.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Deepak Agarwal, Gulshan Kumar, Mohd Ashraf Rather, Ishtiyaq Ahmad
Summary: The complete coding sequence of the Clarias magur SRD5A1 (CmSRD5A1) gene, which regulates steroid levels by converting testosterone into DHT, has been successfully cloned and characterized. The predicted 3D structure of CmSRD5A1 showed high structural similarity with known bacterial and human SRD5As. The study also revealed the expression patterns of SRD5A1 in relation to spawning and preparatory phases in female fish.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Medicinal
Lukas M. Gockel, Vladlena Pfeifer, Fabian Baltes, Rafael D. Bachmaier, Karl G. Wagner, Gerd Bendas, Michael Guetschow, Izidor Sosic, Christian Steinebach
Summary: This research presents a novel approach to treating prostate cancer by exploiting the ubiquitin-proteasome system to degrade specific proteins. By modifying the exit vector, the study successfully demonstrated the potent degradation of androgen receptor in lung cancer cells. These findings provide valuable insights for further investigation into the involvement of androgen receptor in lung cancer models.
ARCHIV DER PHARMAZIE
(2022)