Journal
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
Volume 394, Issue 1-2, Pages 74-85Publisher
ELSEVIER
DOI: 10.1016/j.jembe.2010.07.020
Keywords
partial derivative C-13; C-13 discrimination; CO2 partial pressure; Coccolithophores; Phospholipids-derived fatty acids; PLFA
Categories
Funding
- Agence Nationale de la Recherche [ANR05_BIODIV_004]
- European Project on Ocean Acidificaton (EPOCA)
- European Community [2211384]
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Growth rate, C:N ratio, phospholipid-derived fatty acids (PLEA) distribution and organic carbon isotope composition (partial derivative C-13) of both life stages of the Prymnesiophyceae Emiliania huxleyi (Lohmann), Calcidiscus leptoporus (Murray and Blackman) and Syracosphaera pulchra (Lohman) were analyzed. Cultures were grown at two different CO2 partial pressures (pCO(2), 400 and 760 mu atm) under nutrient-replete conditions. Growth rates (mu) increased at 760 mu atm in all species. C:N ratios were unaffected by pCO(2) in C leptoporus and in the diploid stage of E. huxleyi; they significantly decreased in the haploid stage of E. huxleyi and in both life stages of S. pulchra At 400 mu atm, high similarity was found between the lipid composition of C. leptoporus and S. pulchra differing from that of E. huxleyi. The haploid and diploid life stages showed significant differences in mono- and polyunsaturated C18 fatty acids (MUFA and PUFA), which were more abundant in either stage depending on the species. Except for palmitoleic, vaccenic acids and docosahexaeonic acid (DHA). C leptoporus and S. pulchra fatty acids were lower compared to those of E. huxleyi. Differences in partial derivative C-13 between particulate organic carbon (POC) and PLFA (Delta partial derivative C-13(lipid-POC)) showed common trends at both pCO(2): palmitoleic, oleic acids and DHA were systematically depleted compared to total cell biomass, C18 PUFA were enriched while saturated fatty acids (SAFA) were enriched in C leptoporus and S. pulchra and depleted in E huxleyi. Elevated pCO2 influenced PLFA abundance, Pc of lipids and POC in a highly species-specific way. Fatty acids were generally more depleted at 760 mu atm than at 400 mu atm but the effect was variable among PLEA classes. The relationship between coccolithophore isotopic and PLFA composition and CO2 concentration are analyzed and consequent variations in the future web-chain related to changes in carbonate chemistry are discussed. (C) 2010 Elsevier B.V. All rights reserved.
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