Article
Plant Sciences
Laudecir L. Raiol-Junior, Juan C. Cifuentes-Arenas, Nik J. Cunniffe, Robert Turgeon, Silvio A. Lopes
Summary: The study found that the movement speed of Las in plants is between 2.9-3.8 cm per day, and the colonization of plants by Las is influenced by temperature, with 25.7 degrees Celsius being the optimal condition. This explains the higher impact and incidence of huanglongbing disease during the winter months or regions of milder climates in Brazil.
Article
Plant Sciences
Yongqin Zheng, Jun Guo, Xiaoling Deng, Zheng Zheng
Summary: The draft genome sequence of a 'Candidatus Liberibacter asiaticus' strain isolated from an HLB-affected lime tree in Myanmar was reported, enriching the current genome database and facilitating research on HLB epidemiology in Asia and beyond.
Article
Agronomy
Thais Magni Cavichioli, Maiara Curtolo, Mariangela Cristofani-Yaly, Josiane Rodrigues, Helvecio Della Coletta-Filho
Summary: This study found resistance against Huanglongbing (HLB) in certain citrus hybrid varieties. Long-term observations showed a decrease in infection rate for several hybrids, which also had lower levels of starch accumulation and callose deposition.
Article
Plant Sciences
Hongwei Shi, Zuhui Yang, Jie Huang, Haodi Wu, Shimin Fu, Weimin Li, Xiuping Zou, Changyong Zhou, Xuefeng Wang
Summary: Autophagy is manipulated by the citrus Huanglongbing-associated intracellular bacterium CLas. The CLas effector SDE4405 interacts with host ATG8 proteins to activate autophagy and suppress plant defense responses. This study provides new insights into the interaction between CLas and citrus hosts.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Plant Sciences
Nabil Killiny
Summary: In this study, it was found that the infection of 'Candidatus Liberibacter asiaticus' led to a significant decrease in organic acids in the phloem sap of sweet orange and periwinkle plants. It was hypothesized that bacterial growth induced the mitochondrial tricarboxylic acid cycle to produce these compounds as a carbon source, which may explain the unequal distribution of the bacteria within infected trees.
Article
Plant Sciences
Laudecir L. Raiol-Junior, Juan C. Cifuentes-Arenas, Everton de Carvalho, Eduardo A. Girardi, Silvio A. Lopes
Summary: Las, associated with HLB, moves laterally through phloem in plants, predominantly towards newly developing root and leaf tissues. Protecting rapidly growing shoots from feeding by D. citri is crucial to minimize transmission and spread of the pathogen.
Article
Microbiology
Marcelo L. Merli, Kaylie A. Padgett-Pagliai, Alexandra E. Cuaycal, Lucila Garcia, Maria Rosa Marano, Graciela L. Lorca, Claudio F. Gonzalez
Summary: The study revealed that a potential effector LotP of 'Candidatus Liberibacter asiaticus' may interact with chaperones like GroEL and Hsp40 to enhance Lon protease activity, leading to damage in citrus plant tissues. LotP may also interact with plant proteins, indicating its potential role in protease activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Plant Sciences
Fengnian Wu, Lihui Liu, Eduardo G. P. Fox, Xiaoling Deng, Meirong Xu, Zheng Zheng, Xiuhong Li, Jiawei Fu, Hui Zhu, Jianjian Huang, Tiejun Deng
Summary: This study investigated the effects of 'Ca. L. asiaticus' infection on key physiological variables in citrus plants. Results showed that most variables, except soluble sugar, were affected by increased infection titers, with the variety C. maxima showing greater resistance. The infection was linked to a decrease in chlorophyll concentration and cell membrane integrity, and an increase in free proline and starch contents.
Article
Plant Sciences
P. A. Silva, J. Huang, N. A. Wulff, Z. Zheng, R. Krugner, J. Chen
Summary: This study presents the draft whole-genome sequence of 'Ca. L. asiaticus' strain 9PA from a sweet orange tree in Sao Paulo State, Brazil, providing valuable genetic resources for research and control of HLB.
Article
Plant Sciences
Yanjing Wang, Takumasa Kondo, Yurong He, Zhuohuai Zhou, Jinming Lu
Summary: The study presents the genome sequence of CLas strain CoFLP1 collected from Colombia, contributing to the understanding of the introduction pathway of HLB in South America. This first CLas genome sequence from Colombia will enhance CLas genome resources.
Article
Plant Sciences
Emily M. T. Padhi, Karla J. Araujo, Elizabeth Mitrovic, Marylou Polek, Kris E. Godfrey, Carolyn M. Slupsky
Summary: This study used a metabolomics approach to investigate the changes in leaf metabolites during the early stages of Huanglongbing (HLB). The results showed that infection with Candidatus Liberibacter asiaticus caused significant alterations in leaf metabolome, enabling discrimination between healthy and infected plants.
Article
Microbiology
Lucila Garcia, Maria Celeste Molina, Kaylie Allyson Padgett-Pagliai, Pablo S. Torres, Roberto E. Bruna, Eleonora Garcia Vescovi, Claudio F. Gonzalez, Jose Gadea, Maria Rosa Marano
Summary: This study identifies a putative protease in the pathogen of Huanglongbing disease, which plays an important role in the virulence of the bacteria. Further experiments demonstrate that this protease regulates the shape, exopolysaccharide production, and biofilm formation of the bacteria, affecting their growth and disease development in plant cells.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chunzhen Cheng, Yun Zhong, Bin Wang, Yongyan Zhang, Huan Wu, Nonghui Jiang, Bo Wu, Yuanda Lv, Bo Jiang
Summary: This study found that the upregulated expression of the RIN4 gene in citrus infected with HLB may aid the infection of Candidatus Liberibacter asiaticus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Agustina De Francesco, Amelia H. Lovelace, Dipan Shaw, Min Qiu, Yuanchao Wang, Fatta Gurung, Veronica Ancona, Chunxia Wang, Amit Levy, Tao Jiang, Wenbo Ma
Summary: This study determined the expression profiles of 'Candidatus Liberibacter asiaticus' (Las) genes using a bacterial cell enrichment procedure. The results revealed highly expressed genes in citrus and differentially expressed genes between citrus and Asian citrus psyllids. The study provides insights into the biology of Huanglongbing and the interactions between Las, its plant host, and insect vector.
Article
Agronomy
Chuanyu Yang, Veronica Ancona
Summary: Huanglongbing (HLB) is an important citrus disease caused by an uncultured bacterium called 'Candidatus Liberibacter asiaticus' (CLas). In this study, a method using a dye called propidium monoazide (PMA) was developed to rapidly evaluate the effectiveness of HLB therapies in field experiments. The PMA-qPCR method was able to differentiate between live and dead bacteria, and its effectiveness depended on the primer set used. Application of this method in field tests showed that certain antibiotics displayed significant antibacterial activity against CLas, which was not detected by traditional qPCR analysis. This method could facilitate the validation of treatments against HLB and the implementation of effective management strategies.
Correction
Agronomy
Patrick H. Brown, Fang-Jie Zhao, Achim Dobermann
Article
Plant Sciences
Douglas C. Amaral, Patrick H. Brown
Summary: The objective of this research was to investigate the effects of an inositol-based plant stimulant on the mobility and accumulation of foliar-applied zinc in wheat plants. The results showed that the inclusion of myo-inositol with Zinc sulfate significantly increased zinc concentration in shoots and grains, suggesting that foliar application of myo-inositol in combination with zinc may be a promising strategy to enhance zinc accumulation in grains.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Green & Sustainable Science & Technology
Evie Smith, Lisa Antoshak, Patrick H. Brown
Summary: Smallholder coffee producers are facing challenges threatening the sustainability of the specialty coffee industry. Collaboration between researchers and stakeholders is essential to address social, economic, and environmental threats. By incorporating farmers' voices and addressing key challenges like pests, diseases, climate change, and market access, the long-term sustainability of the industry can be improved.
Article
Biology
Jun Ge, Jiayu Lin, Zhiying Wu, Kuan Xu, Jingyu Tao, Haizhong Lin, Shengke Tian, Lingli Lu
Summary: This study identified the function of SaPCR2 in the zinc uptake of S. alfredii through gene expression analysis and transgenic plant experiments. The results showed that SaPCR2 was highly expressed in the root elongation zone and significantly increased the zinc uptake in transgenic plants. Additionally, overexpression of SaPCR2 altered root morphology and inhibited root growth.
Article
Environmental Studies
Liza Wood, Mark Lubell, Jessica Rudnick, Sat Darshan S. Khalsa, Molly H. Sears, Patrick Brown
Summary: This article evaluates the impact of a nitrogen management policy in California on farmer learning. The study finds that early conceptual learning is driven by farmers' awareness of environmental impact and positive perceptions of regulators, while later stage applied learning is primarily influenced by high levels of informational support. Additionally, lower-resourced farmers benefit from mandatory plan-writing. Therefore, policies for conservation behavior should provide tailored support according to different learning stages.
Article
Environmental Sciences
Jun Ge, Jingyu Tao, Jianqi Zhao, Zhiying Wu, Hewan Zhang, Yuxiao Gao, Shengke Tian, Ruohan Xie, Shengyang Xu, Lingli Lu
Summary: Sedum alfredii Hance, a Cd/Zn hyperaccumulator native to China, shows high tolerance to Pb, Cu, Ni, and Mn, making it valuable for phytoremediation of soils contaminated with multiple heavy metals. Transcriptomic analysis reveals unique and flexible strategies employed by this species in response to different heavy metals, with some genes found to be associated with heavy metal tolerance for the first time.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Plant Sciences
Gustavo F. de Sousa, Maila Adriely Silva, Everton G. de Morais, Gustavo Avelar Z. Van Opbergen, Guilherme Gerrit A. Z. Van Opbergen, Raphael R. de Oliveira, Douglas Amaral, Patrick Brown, Antonio Chalfun-Junior, Luiz Roberto Guimaraes Guilherme
Summary: This study aimed to evaluate the effects of foliar selenium application on coffee seedlings submitted to chilling stress. The results showed that selenium reduced leaf injury, changed metabolic processes, and increased antioxidant enzyme activity in response to low temperature. However, the response of Coffea arabica and Coffea canephora to selenium and chilling stress differed.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Environmental Sciences
Tracey J. Woodruff, Swati D. G. Rayasam, Daniel A. Axelrad, Patricia D. Koman, Nicholas Chartres, Deborah H. Bennett, Linda S. Birnbaum, Phil Brown, Courtney C. Carignan, Courtney Cooper, Carl F. Cranor, Miriam L. Diamond, Shari Franjevic, Eve C. Gartner, Dale Hattis, Russ Hauser, Wendy Heiger-Bernays, Rashmi Joglekar, Juleen Lam, Jonathan I. Levy, Patrick M. MacRoy, Maricel V. Maffini, Emily C. Marquez, Rachel Morello-Frosch, Keeve E. Nachman, Greylin H. Nielsen, Catherine Oksas, Dimitri Panagopoulos Abrahamsson, Heather B. Patisaul, Sharyle Patton, Joshua F. Robinson, Kathryn M. Rodgers, Mark S. Rossi, Ruthann A. Rudel, Jennifer B. Sass, Sheela Sathyanarayana, Ted Schettler, Rachel M. Shaffer, Bhavna Shamasunder, Peggy M. Shepard, Kristin Shrader-Frechette, Gina M. Solomon, Wilma A. Subra, Laura N. Vandenberg, Julia R. Varshavsky, Roberta F. White, Ken Zarker, Lauren Zeise
Summary: The manufacture and production of industrial chemicals continues to increase, leading to widespread population exposures and resultant health impacts. Low-wealth communities and communities of color often bear disproportionate burdens of exposure and impact. Multiple authoritative bodies and scientific consensus groups have called for actions to prevent harmful exposures via improved policy approaches. We developed consensus recommendations for health-protective, scientific approaches to reduce harmful chemical exposures, which can be applied to current US policies governing industrial chemicals and environmental pollutants.
ENVIRONMENTAL HEALTH
(2023)
Article
Environmental Sciences
Jianqi Zhao, Ruohan Xie, Jiayu Lin, Lingling Xu, Xiaoyu Gao, Xianyong Lin, Shengke Tian, Lingli Lu
Summary: This study functionally characterized SaMT3 in the Cd/Zn hyperaccumulator S. alfredii. The C- and N-terminal regions of SaMT3 exhibited differential functional roles in Cd adaptation, with the C-terminal region involved in Cd sensing and binding and the N-terminal region responsible for ROS scavenging. Overexpression of SaMT3 increased Cd tolerance in transgenic plants, while knockout of SaMT3 led to decreased Cd concentrations and increased ROS levels. These findings provide valuable insights into the role of SaMT3 in Cd hyperaccumulation and the molecular mechanisms underlying Cd detoxification in plants.
ENVIRONMENTAL POLLUTION
(2022)
Article
Engineering, Environmental
Lingling Xu, Shengke Tian, Yan Hu, Jun Ge, Jianqi Zhao, Lingli Lu
Summary: This study found that Cd can improve the heat resistance of the hyperaccumulator plant Sedum alfredii. Cd penetrates the guard cells, restores stomatal function, and mitigates water loss under heat stress. Additionally, Cd activates antioxidant enzymes, promotes phytohormone biosynthesis, and upregulates a series of genes, enhancing the heat resistance of S. alfredii.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Engineering, Environmental
Marcia E. A. Carvalho, Evgenios Agathokleous, Marina L. Nogueira, Gustavo Brunetto, Patrick H. Brown, Ricardo A. Azevedo
Summary: This review analyzes the impacts of Cd on seed germination and seedling vigor and the use of priming agents to mitigate these impacts. Results show that seed endurance to Cd exposure varies among species, with some seeds maintaining or improving their germination and vigor. Factors influencing seed tolerance include temporal variations in anatomical, physiological, and biochemical features. Cd can also have transgenerational effects on plants by affecting seed endophytes, provisioning of resources and regulatory elements, and seed (epi)genomics. Different priming agents can mitigate Cd-induced impacts on seeds, sometimes even enhancing their performance. Overall, understanding plant tolerance mechanisms against Cd pollution is important for breeding programs, agriculture, and environmental policies.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Soil Science
Chieh Yu, Daniel J. Geisseler, Patrick H. Brown, Sat Darshan S. Khalsa
Summary: This study evaluates the distribution of potassium (K), calcium (Ca), and magnesium (Mg) in orchard soils following four wetting events. The results show differences in the distribution of K, Ca, and Mg among different soil types, with higher concentrations in sandy loam soils and lower concentrations in loam soils. The study provides insights into the availability of potassium fertilizer for orchard soils.
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL
(2023)
Article
Plant Sciences
Zhehan Tang, Yufang Jin, Patrick H. Brown, Meerae Park
Summary: Tracking plant water status is essential for precision irrigation management of processing tomatoes. This study demonstrates the use of proximal sensors and high-resolution UAV imagery to efficiently monitor crop water status. Machine learning models accurately estimate tomato stem water potential by integrating PRI, other vegetation indices, and weather data, facilitating data-driven irrigation management.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Plant Sciences
Minmeng Tang, Dennis Lee Sadowski, Chen Peng, Stavros G. Vougioukas, Brandon Klever, Sat Darshan S. Khalsa, Patrick H. Brown, Yufang Jin
Summary: This study demonstrates the successful use of deep learning and multi-spectral imaging technology to predict the yield of individual almond trees. The results show that the deep learning model accurately predicts the variation in tree-level yield, with the reflectance at the red edge band playing an important role in the prediction. This research highlights the potential of deep learning for data-driven site-specific resource management in agriculture sustainability.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Food Science & Technology
Maila Adriely Silva, Gustavo Ferreira de Sousa, Gary Banuelos, Douglas Amaral, Patrick H. Brown, Luiz Roberto Guimaraes Guilherme
Summary: This study aimed to evaluate the effect of different selenium application methods (soil or foliar) and sources (organic or inorganic) on the total selenium content and speciation in selenium-enriched soybean grains. The results showed that all treatments with inorganic selenium increased the selenium content in grains compared to the control. More than 80% of the total selenium was present as selenomethionine (SeMet), and the speciation was influenced by the selenium source and application method. The treatments using inorganic selenium, applied via soil or foliar, produced the highest content of SeMet in soybean grains. Finally, the preservation of selenium species in products derived from soybean grains should be evaluated.
