Article
Nutrition & Dietetics
Meijuan Bai, Peijuan Cao, Yijun Lin, Pengcheng Yu, Shuo Song, Lingling Chen, Lan Wang, Yan Chen
Summary: Short-term caloric restriction (CR) promotes erythropoiesis and ameliorates phenylhydrazine-induced acute anemia.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Jinbo Li, Yuanyuan Li, Min Zhu, Shilin Song, Zhanfen Qin
Summary: In this study, a rapid enzyme-linked immunosorbent assay (ELISA) was developed to screen thyroid hormone signaling disruptors. The T3 concentration was first screened, followed by testing two known disruptors and detecting several suspected disruptors. The results demonstrated that TH signaling disruptors can be rapidly screened out by evaluating the expression of the thibz gene as a sensitive endpoint.
Article
Cell Biology
Lei Li, Ying Xu, Jihong Zheng, Zhe Kuang, Cong Zhang, Na Li, Gufa Lin, Chao Zhang
Summary: The study revealed a specific regulatory pattern of the melanocortin system in Xenopus laevis, where MRAP2 proteins directly interact with MC4R proteins on the cell surface in a functional antiparallel dimeric topology.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Review
Hematology
Pu Tang, Huaquan Wang
Summary: Erythropoiesis is a complex process involving the transfer of cells from the yolk sac to the fetal hepar and then to the bone marrow. Erythroid progenitor cells in the bone marrow undergo stages of development to produce reticulocytes that enter the bloodstream. Various factors, including erythropoietin and hepcidin, regulate erythropoiesis. Disruptions in these factors can lead to abnormal red blood cell production and function. Understanding erythropoiesis is important for diagnosing and treating hematological disorders.
Article
Biochemistry & Molecular Biology
Alexander A. Tokmakov, Ken-Ichi Sato
Summary: This study found that intracellular ROS levels increase in aged frog oocytes and eggs, and that targeted inhibition of NADPH oxidase with apocynin significantly reduces ROS levels, maintaining normal cell function.
Letter
Biochemistry & Molecular Biology
Shouhong Wang, Yun-Bo Shi
Summary: Tissue regeneration is crucial for biomedical developments, but many human organs cannot regenerate. Research shows that there is a significant difference in tail regeneration between Xenopus laevis and the closely related species Xenopus tropicalis, with Xenopus laevis experiencing a refractory period while Xenopus tropicalis does not. Further studies in Xenopus tropicalis may provide insights into the genetic basis of this evolutionary divergence and how tissue regenerative capacity is controlled, with implications for human regenerative medicine.
CELL AND BIOSCIENCE
(2021)
Review
Biology
Yelena Ginzburg, Xuili An, Stefano Rivella, Adam Goldfarb
Summary: Erythroblasts undergo differentiation to become mature red blood cells, requiring large amounts of iron for hemoglobin synthesis. Recent evidence reveals the regulation of iron availability by erythroblasts and the effects of insufficient or excess iron on erythroid lineage proliferation and differentiation. Dysregulated iron metabolism is found in various congenital and acquired diseases. Understanding this mechanism provides opportunities to improve the management of patients with disordered erythropoiesis.
Article
Chemistry, Analytical
Luise Fast, Richard Agren, Hugo Zeberg
Summary: This study explored the ability of Xenopus laevis oocytes to express membrane-bound enzymes and characterized their enzymatic activity using fluorescence-based assays. The results suggest that Xenopus oocytes can be used for functional evaluation of membrane-bound enzymes, reducing experimental workload.
Article
Cell & Tissue Engineering
Valerio Brizi, Sara Butto, Domenico Cerullo, Angelo Michele Lavecchia, Raquel Rodrigues-Diez, Rubina Novelli, Daniela Corna, Ariela Benigni, Giuseppe Remuzzi, Christodoulos Xinaris
Summary: The study revealed the ability of human NCCs to produce functional EPO in response to hypoxia, providing a new approach for cell therapy for renal and non-renal anemia.
STEM CELL RESEARCH
(2021)
Article
Engineering, Environmental
Meng Li, Meile Lv, Tingting Liu, Gaoyi Du, Qangwei Wang
Summary: In this study, exposure to cis-BF at environmentally relevant concentrations resulted in lipid metabolic disorder and nonalcoholic fatty liver disease in Xenopus laevis, providing new insights into the potential long-term hazards of pyrethroids.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Environmental Sciences
Hui Jiao, Tingting Yuan, Xiaohuan Wang, Xia Zhou, Renyue Ming, Honghao Cui, Deyu Hu, Ping Lu
Summary: This study assessed the toxic effects of acetamiprid on Xenopus laevis and found that it had detrimental effects on the liver, causing oxidative stress and histological damage. Metabolomic analysis revealed disrupted metabolic pathways, indicating the hepatotoxic effects of acetamiprid on X. laevis at a molecular level. These findings are crucial for evaluating the aquatic risks of neonicotinoids.
ENVIRONMENTAL POLLUTION
(2023)
Article
Environmental Sciences
Mohy Eldin Abd-El-Fattah, Amina A. Dessouki, Noha S. Abdelnaeim, Bassant M. Emam
Summary: The study evaluated the anti-anemic effect of beetroot supplement on anemia induced by phenylhydrazine in albino rats. Results showed that beetroot supplementation ameliorated alterations caused by hemolytic anemia, with beetroot powder showing more powerful health effects compared to beetroot extract and iron preparation.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Michelle Leemans, Petra Spirhanzlova, Stephan Couderq, Sebastien Le Mevel, Alexis Grimaldi, Evelyne Duvernois-Berthet, Barbara Demeneix, Jean-Baptiste Fini
Summary: Thyroid hormones (TH) are crucial for brain development, but endocrine-disrupting chemicals (EDCs) in the environment can potentially interfere with TH signaling and impact neurodevelopment and behavior. This study investigated the effects of an amniotic mixture containing commonly found EDCs on brain development and TH signaling using Xenopus laevis tadpoles. The results showed that while there were similarities in TH-dependent genes between thyroxine (T-4) and the amniotic mixture, they also had distinct gene signatures. T-4-treated tadpoles showed increased brain cell proliferation, whereas the amniotic mixture had no effect. Additionally, both T-4 and mixture exposure resulted in decreased tadpole motility. These findings highlight the importance of examining the effects of chemical mixtures for better risk assessment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell & Tissue Engineering
Martina Rauner, Marta Murray, Sylvia Thiele, Deepika Watts, Drorit Neumann, Yankel Gabet, Lorenz C. Hofbauer, Ben Wielockx
Summary: High levels of erythropoietin (Epo) can be harmful to bone health in adult organisms, and EpoR in osteoprogenitor cells plays a crucial role in regulating osteoblast function and osteoblast-mediated osteoclastogenesis.
Article
Biochemistry & Molecular Biology
Vishnu V. Krishnamurthy, Hyojeong Hwang, Jia Fu, Jing Yang, Kai Zhang
Summary: Optogenetics utilizes light-inducible protein-protein interactions to control signaling activity with precision, offering attractive spatial and temporal resolution for studying embryonic development. In a comparative study, a cytoplasm-to-membrane translocation-based optogenetic system outperformed membrane-anchored dimerization systems in activating receptor tyrosine kinase signaling in live Xenopus embryos. This engineering strategy may have broad applicability to other signaling pathways involving membrane-bound receptors, as demonstrated by triggering Wnt activity through optogenetic activation of LRP6.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Kei Sato, Azusa Uehara, Sayaka Kinoshita, Ikki Nomura, Minami Yagi, Yuta Tanizaki, Yu Matsuda-shoji, Atsushi Matsubayashi, Nobuyasu Endo, Yutaka Nagai, Takashi Kato
SCIENTIFIC REPORTS
(2018)
Article
Biochemistry & Molecular Biology
Shigeki Arai, Chie Shibazaki, Motoyasu Adachi, Yoshitake Maeda, Tomoyuki Tahara, Takashi Kato, Hiroshi Miyazaki, Ryota Kuroki
Article
Fisheries
Shun Maekawa, Pei-Chi Wang, Shih-Chu Chen
FISH & SHELLFISH IMMUNOLOGY
(2019)
Article
Immunology
Theeraporn Pulpipat, Shun Maekawa, Pei-Chi Wang, Shih-Chu Chen
Article
Fisheries
Yoshie Takahashi, Yo Okamura, Natsuki Morimoto, Koshin Mihara, Shun Maekawa, Han-Ching Wang, Takashi Aoki, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima
FISH & SHELLFISH IMMUNOLOGY
(2020)
Review
Fisheries
Shun Maekawa, Yi-Ting Wang, Terutoyo Yoshida, Pei-Chi Wang, Shih-Chu Chen
JOURNAL OF FISH DISEASES
(2020)
Article
Fisheries
Natsuki Morimoto, Yo Okamura, Shun Maekawa, Han-Ching Wang, Takashi Aoki, Tomoya Kono, Masahiro Sakai, Jun-ichi Hikima
FISH & SHELLFISH IMMUNOLOGY
(2020)
Article
Fisheries
Shun Maekawa, Theeraporn Pulpipat, Pei-Chi Wang, Shih-Chu Chen
Summary: The study investigated the gene expression responses to Fno infection in Nile tilapia by analyzing the transcriptome of the head kidney and spleen. Differentially expressed genes were significantly enriched in immune-related pathways. Additionally, intracellular iron storage in the spleen causing anemia of inflammation after Fno infection was observed.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Cell Biology
Shiori Yamada-Kondo, Ayame Ogawa, Miku Fukunaga, Yumi Izutsu, Takashi Kato, Mitsugu Maeno
Summary: In this study, a myeloid cell-specific enhancer was identified in the 5' flanking region of the Xenopus tropicalis myeloperoxidase gene. Using transgenic reporter analysis, myeloid cells in tadpoles were successfully labeled with GFP, allowing for the study of their physiological role. The results showed that GFP-positive cells were predominantly found in tail fin macrophages and liver myeloid cells, providing valuable insights into the distribution and function of myeloid cells in Xenopus tadpoles.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2022)
Article
Cell Biology
Shingo Fujiyama, Takehito Okui, Takashi Kato
Summary: This study aimed to develop an experimental model on intermittent hypoxia in African clawed frogs (Xenopus laevis). The results showed that this model can be used to evaluate changes in hypoxia and oxidative stress in X. laevis. It was found that the degree of tissue damage depended on the affected tissues.
DEVELOPMENT GROWTH & DIFFERENTIATION
(2023)
Article
Fisheries
Andre Giovanni, Shun Maekawa, Pei-Chi Wang, Shih-Chu Chen
Summary: In this study, the VhFliA gene was isolated from Vibrio harveyi and its in vivo activities were investigated. The results showed that VhFliA could induce the expression of inflammatory cytokines in orange-spotted grouper and koi carp, demonstrating its potential as an effective immune stimulant adjuvant.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shun Maekawa, Pei-Chi Wang, Shih-Chu Chen
Summary: In this study, transcriptome analysis of the head kidney and spleen of the fourfinger threadfin fish infected with Streptococcus iniae revealed differentially expressed genes (DEGs) involved in immune-related pathways. The DEGs were found to be enriched in pathways such as phagosome, Th1 and Th2 cell differentiation, and cytokine-cytokine receptor interactions, indicating their important roles in the immune response against S. iniae infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Ichiro Takeuchi, Kanako Tanase-Nakao, Ayame Ogawa, Tohru Sugawara, Osuke Migita, Makoto Kashima, Touko Yamazaki, Akihiro Iguchi, Yasuhiro Naiki, Toru Uchiyama, Junya Tamaoki, Hiroki Maeda, Hirotaka Shimizu, Toshinao Kawai, Kosuke Taniguchi, Hiromi Hirata, Makoto Kobayashi, Kimikazu Matsumoto, Kiyoshi Naruse, Kenichiro Hata, Hidenori Akutsu, Takashi Kato, Satoshi Narumi, Katsuhiro Arai, Akira Ishiguro
Summary: Mutations in the POLE gene have been found to be associated with a rare form of severe congenital transfusion-dependent anemia, characterized by abnormal hematopoiesis and bone marrow dysplasia.
JOURNAL OF MEDICAL GENETICS
(2023)
Article
Biology
Kazuki Omata, Ikki Nomura, Akito Hirata, Yuka Yonezuka, Hiroshi Muto, Ryo Kuriki, Kirin Jimbo, Koujin Ogasa, Takashi Kato
Summary: Xenopus liver maintains erythropoietic activity from larval to adult stage. During metamorphosis, a globin switch occurs, and the whole-body mass and liver also change. We developed a method to isolate and evaluate erythroid progenitors using monoclonal ER9 antibodies and Acridine orange staining. Our findings show increased erythropoiesis in the froglet liver and demonstrate growth-dependent changes in erythropoiesis patterns in specific organs of Xenopus.
Review
Immunology
Shun Maekawa, Pei-Chi Wang, Shih-Chu Chen
FRONTIERS IN IMMUNOLOGY
(2019)