Article
Oncology
Francesca Liccardo, Martyna Sniegocka, Claudia Tito, Alessia Iaiza, Tiziana Ottone, Mariadomenica Divona, Serena Travaglini, Maurizio Mattei, Rosella Cicconi, Selenia Miglietta, Giuseppe Familiari, Stefania Annarita Nottola, Vincenzo Petrozza, Luca Tamagnone, Maria Teresa Voso, Silvia Masciarelli, Francesco Fazi
Summary: In this study, a combination therapy of low doses of retinoic acid (R), bortezomib (B), and arsenic trioxide (A) was found to induce proteotoxic stress and oxidative stress in FLT3-ITD+ AML cells, resulting in strong cytotoxic activity. The protective role of bone marrow stromal cells (BMSCs) in AML cells was also demonstrated, but this protection can be overcome by using high doses of ascorbic acid (Vitamin C) as an adjuvant. Importantly, the combination of RBA and ascorbic acid significantly prolonged the lifespan of a murine model of FLT3-ITD+ AML without toxic effects.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Article
Cell Biology
Tong-Dian Zhang, Yu-Bo Ma, Ming Gao, He-Cheng Li, Zi-Ming Wang, Tie Chong, Lian-Dong Zhang
Summary: This study modified the agarose organ culture system and found that it could better mimic the testicular microenvironment. Low dose of MEHP caused mild disruption to fetal testis development, but co-treatment with a low dose of genistein attenuated the MEHP-induced testis injuries partially by balancing the redox state.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Chemistry, Analytical
Apresio K. Fajrial, Kun Liu, Yu Gao, Junhao Gu, Richard Lakerveld, Xiaoyun Ding
Summary: Characterization of physical biomarkers of cells through osmotic swelling can evaluate unique swelling dynamics of different cell types with high accuracy. This method can also detect changes in cellular mechanical properties due to cytoskeleton disruption, offering potential applications in both biological studies and clinical practice. Its simplicity makes swelling dynamics an invaluable label-free physical biomarker for cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Hui Guo, Tianci Chen, Zhi Liang, Lanfen Fan, Yuchun Shen, Dayan Zhou
Summary: This study elucidated the proteomic responses of white shrimp under copper stress, revealing disruptions in ion homeostasis and protein synthesis. It was found that the shrimp mainly regulates a series of molecular pathways involved in immune processes to protect the organism from copper stress. These findings provide insight into the underlying mechanisms related to the stress response of copper exposure in crustaceans.
Article
Forestry
Qingjun Xie, Yuanyuan Wang, Danni Wang, Jinghang Li, Baichao Liu, Zhongyuan Liu, Peilong Wang, Hao Zhang, Kaiye Yang, Caiqiu Gao
Summary: This study used RNA-seq to identify differentially expressed genes in Tamarix hispida roots treated with CdCl2. The analysis found that these genes were associated with defense response and metabolic pathways in plants. Furthermore, the study revealed the important regulatory role of certain transcription factors in the response to cadmium stress.
Article
Multidisciplinary Sciences
Bianca Sayuri Takayasu, Sheila Silva Rodrigues, Carlos Eduardo Madureira Trufen, Glaucia Maria Machado-Santelli, Janice Onuki
Summary: Snake envenomation is a neglected tropical disease, with Bothrops genus accounting for a large portion of snakebite accidents in Brazil. This study aimed to analyze the cytotoxicity and molecular mechanisms of Bothrops spp. venoms on rat liver cells. The results showed that the venoms decreased cell viability and disrupted microtubules and actin network, without causing cell cycle arrest. No clear correlation was found between the IC50 values and enzymatic/biological activities of the venoms. This work contributes to understanding and improving the treatment of snake envenomation.
Article
Cell Biology
Qianqian Ge, Jiajia Wang, Jitao Li, Jian Li
Summary: This study used single cell RNA-sequencing to investigate the specific responses of shrimp gill cells to high pH stress. The results showed that pillar cells were the main target cell type in response to high pH stress, exhibiting immune activation and stress defense states. Additionally, the study predicted the regulatory network of the pillar cell population and identified two transcription factors.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
Benjamin Allcock, Wenbin Wei, Kirsty Goncalves, Henry Hoyle, Alisha Robert, Rebecca Quelch-Cliffe, Adam Hayward, Jim Cooper, Stefan Przyborski
Summary: Cells respond to their microenvironment, and this has implications for cell culture practices. In this study, the cellular processes that occur when a cell is cultured on 2D or 3D surfaces for a short period of time were examined. The study found that this priming process improved the cell's functionality and drug toxicity profiles. The findings have broad significance in the field of cell biology, demonstrating the fundamental impact of mechanotransduction in bioengineering and cell biology.
Article
Agriculture, Dairy & Animal Science
Huakun Zhang, Ziwen Hu, Run Li, Yaohui Wang, Jinxu Zhou, Hao Xu, Guan Wang, Xuemei Qiu, Xiuli Wang
Summary: This study explores the molecular mechanism of hypoxia tolerance in Takifugu obscurus by analyzing changes in metabolites in the gill tissue under acute hypoxia. The findings provide a scientific basis for controlling dissolved oxygen levels in the aquaculture environment and for breeding new varieties with low oxygen tolerance.
Article
Immunology
Lilan Sun, Lang Sun, Xue Li, Xinxin Hu, Xiukun Wang, Tongying Nie, Youwen Zhang, Xuefu You
Summary: ML-7 hydrochloride (ML-7) has been identified as a potential novel tigecycline adjuvant to combat tigecycline-resistant Klebsiella pneumoniae. ML-7 exhibits synergistic bactericidal activities with tigecycline, inhibits efflux pump function, disrupts proton motive force, reduces intracellular ATP levels, and leads to accumulation of ROS. Furthermore, transcriptomic analysis reveals the enrichment of ML-7-related genes in ABC transporters.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Beatriz Ferrer, Harshini Suresh, Abel Santamaria, Joao Batista Rocha, Aaron B. Bowman, Michael Aschner
Summary: This study found that exposure to MeHg can induce STAT3 activation in mouse hypothalamic neuronal cells, exacerbating oxidative stress and antioxidant responses. It suggests that the STAT3 signaling pathway plays an important role in responding to MeHg-induced oxidative stress.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Microbiology
Keng Po Lai, Peng Zhu, Delbert Almerick T. Boncan, Lu Yang, Cherry Chi Tim Leung, Jeff Cheuk Hin Ho, Xiao Lin, Ting Fung Chan, Richard Yuen Chong Kong, William Ka Fai Tse
Summary: This study is the first to report the hypotonic responsive genes in gill cells and the compositions of gill microbiota in marine medaka using transcriptome and 16S rRNA gene sequencing. The overlapping glycosaminoglycan- and chitin-related pathways suggest host-bacterium interaction in fish gill during osmotic stress.
Review
Biochemistry & Molecular Biology
Caihong Wang, Kai Qu, Jing Wang, Rui Qin, Bingyi Li, Juhui Qiu, Guixue Wang
Summary: Cell polarity, especially in endothelial cells, plays a crucial role in various diseases and is regulated by biomechanical forces.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Review
Biochemistry & Molecular Biology
Caihong Wang, Kai Qu, Jing Wang, Rui Qin, Bingyi Li, Juhui Qiu, Guixue Wang
Summary: This article mainly introduces the mechanism of cell polarity in endothelial cells and related diseases, pointing out the regulatory role of biomechanical forces on endothelial cell polarity, providing new ideas for the treatment of PCP-related diseases in endothelial cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2022)
Article
Pharmacology & Pharmacy
Patricia Sinclair, Nadine Kabbani
Summary: This article reviews the evidence of ionotropic and metabotropic signaling responses of α7 nAChRs in various cell types, and explores the importance of these signaling responses in regulating cytoskeletal motility and cell growth.
PHARMACOLOGICAL RESEARCH
(2023)