Article
Multidisciplinary Sciences
Yuan Wen, Davis A. Englund, Bailey D. Peck, Kevin A. Murach, John J. McCarthy, Charlotte A. Peterson
Summary: The study revealed that satellite cells in muscle are activated during acute exercise, but show limited lineage progression during muscle adaptation. Nuclei without satellite cells demonstrate divergent transcriptional dynamics compared to muscles with sufficient satellite cells.
Article
Sport Sciences
Michaela Wehrstein, Axel Schoeffel, Nadine Weiberg, Thomas Gwechenberger, Theresa Betz, Mareike Rittweg, Mario Parstorfer, Maximilian Pilz, Birgit Friedmann-Bette
Summary: The study aimed to assess if one bout of concentric/eccentric exercise with damaging eccentric overload provides a sufficient stimulus to induce satellite cell (SC) activation, proliferation, and differentiation. The results showed that eccentric overload during leg extension exercise induced significant SC activation and increases in SC content, but there were no signs of increased SC differentiation or formation of new myofibers.
MEDICINE & SCIENCE IN SPORTS & EXERCISE
(2022)
Article
Food Science & Technology
Limei Chen, Baohua Qu, Hui Wang, Hongning Liu, Yongmei Guan, Juanqing Zhou, Jiaqi Zhang
Summary: The study investigated the effects of Xianmao on kidney energy metabolism in rats, finding that Xianmao significantly increased enzyme activity and ATP content in the kidney, potentially mediated by various metabolic pathways and upregulation of specific genes.
FOOD SCIENCE & NUTRITION
(2021)
Article
Veterinary Sciences
Tong Qin, Jing Wang, Shang-Jin Cui
Summary: nanoPCR is a novel method for the specific detection of viruses, with high sensitivity and specificity for different strains of canine coronavirus. The assay showed a 100-fold higher sensitivity compared to traditional RT-PCR, and successfully detected CCoV I and CCoV II in clinical samples with high positivity rates.
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
(2021)
Article
Multidisciplinary Sciences
Thaer S. A. Abdalla, Jan Meiners, Sabine Riethdorf, Alexandra Koenig, Nathaniel Melling, Tobias Gorges, Karl-F. Karstens, Jakob R. Izbicki, Klaus Pantel, Matthias Reeh
Summary: The detection of circulating tumor cells in patients with colorectal cancer before surgery is an independent and strong prognostic factor for overall survival. This study suggests that it could improve the identification of high-risk patients in future clinical trials.
Article
Biochemistry & Molecular Biology
Michela Cantiello, Monica Carletti, Mery Giantin, Giulia Gardini, Francesca Capolongo, Paolo Cascio, Marianna Pauletto, Flavia Girolami, Mauro Dacasto, Carlo Nebbia
Summary: This study provides a comprehensive characterization of phenobarbital's (PB) effects on drug-metabolizing enzyme (DME) catalytic activities in cattle liver. The findings show that PB increases the protein amounts and enzyme activities of certain target enzymes, but has contradictory effects on others. The study also reveals an increase in 26S proteasome activity, suggesting a potential role in cattle DME regulation. The results further confirm species differences in DME expression and activity between cattle, humans, and rodents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Health Care Sciences & Services
Jiaqi Bi, Hong Jing, ChenLiang Zhou, Peng Gao, Fujun Han, Gang Li, Shiwei Zhang
Summary: This study found that different magnetic flux densities of low-frequency electromagnetic field (LF-EMF) have varying positive effects on different stages of skeletal myogenesis, including satellite cell activation and proliferation, muscle cell differentiation, and myocyte fusion.
TECHNOLOGY AND HEALTH CARE
(2022)
Article
Biochemistry & Molecular Biology
Karuppiah Hilda, Sreeramulu Bhuvaragavan, Ramakrishnan Kamatchi, Mani Meenakumari, Sundaram Janarthanan
Summary: The study successfully demonstrated the effective insecticidal activity against the bruchid beetle by cloning and expressing the arcelin gene from wild sources, and elucidated the mechanism by studying the gene expression of digestive enzymes.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Plant Sciences
F. Qalavand, M. Nasr-Esfahani, J. Vatandoost, D. A. Azarm
Summary: In wheat, Bipolaris sorokiniana is an important pathogen causing global yield losses. This study screened 33 wheat genotypes and analyzed the expression of candidate defense genes and the activity of antioxidant enzymes. The results showed that resistant genotypes had upregulated defense genes and increased antioxidant enzyme activity. This research sheds light on the mechanisms of resistance in wheat cultivars and their simultaneous resistance to multiple diseases.
Article
Plant Sciences
Dhananjay A. Hongal, Dhandapani Raju, Sudhir Kumar, Akshay Talukdar, Anjan Das, Khushboo Kumari, Prasanta K. Dash, Viswanathan Chinnusamy, Anilabha Das Munshi, Tusar Kanti Behera, Shyam Sundar Dey
Summary: Cucumber genotypes with different responses to temperature stress were evaluated, and the study found that heat tolerance in cucumber is associated with physiological traits such as chlorophyll retention, membrane stability, water content, net photosynthesis, stomatal conductance, transpiration, as well as the accumulation of biochemicals and the upregulation of heat shock proteins and photosynthesis-related genes. The findings provide a foundation for the design of climate smart cucumber genotypes through integration of favorable physiological, biochemical, and molecular traits related to heat stress tolerance.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Cell Biology
Hongye Wang, Yile Huang, Ming Yu, Yang Yu, Sheng Li, Huating Wang, Hao Sun, Bing Li, Guoliang Xu, Ping Hu
Summary: Tet2 plays a crucial role in muscle regeneration by regulating myoblast differentiation and fusion. It activates Myogenin transcription, rescuing differentiation and fusion defects in Tet2 knockout myoblasts. Tet2 enhances MyoD binding by demethylating flanking CpG sites, increasing chromatin accessibility and activating transcription.
CELL DEATH & DISEASE
(2021)
Article
Integrative & Complementary Medicine
Sang Hee Park, Dong Seon Kim, Jieun Oh, Jeong-Ho Geum, Jung-Eun Kim, Su-Young Choi, Ji Hye Kim, Jae Youl Cho
Summary: Chamomile extract MC shows significant effects on muscle atrophy by recovering muscle volume, density, and weight loss, increasing moving distance and grip strength. Its mechanism of action may involve inhibition of MuRF1 mRNA level, and enhancement of TFAM, MyoD, and Myogenin-1 expression.
AMERICAN JOURNAL OF CHINESE MEDICINE
(2021)
Article
Polymer Science
Cai-Hong Li, Ya-Ning Liu, Hao Zhang, Zhen Shi
Summary: In this study, two coordination polymers based on Co(II) were synthesized using solvothermal method, with different nitrogen-donor ligands. The treatment effect of these compounds on postpartum depression was assessed, and the underlying mechanism was investigated.
Article
Plant Sciences
Shatil Arabia, Md Nur Ahad Shah, Asif Ahmed Sami, Ajit Ghosh, Tahmina Islam
Summary: Proline is utilized by organisms as a stress adaptor molecule to offset cellular imbalances caused by environmental stresses. Comprehensive analysis of proline metabolizing genes is essential for understanding their function and regulation. Validation of candidate genes in rice using in-silico data provides insights on their involvement in stress response and potential for metabolic engineering to develop stress-resilient plants.
PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
(2021)