Article
Biochemistry & Molecular Biology
Yuri Lee, Hai-long Piao, Jongchan Kim
Summary: The Wnt signaling pathway is crucial for regulating various cellular processes, and dysregulation of this pathway has been linked to human diseases, including cancer. In this study, researchers aimed to identify the deubiquitinases (DUBs) that regulate the pathway through the essential component LEF1. They discovered that OTUD7B interacts with LEF1 and activates Wnt signaling. Furthermore, OTUD7B promotes the nuclear localization of LEF1, leading to increased interaction with beta-catenin. This study suggests that OTUD7B may serve as a potential therapeutic target in diseases where Wnt signaling is dysregulated, such as cancer.
Article
Biochemistry & Molecular Biology
Shaopu Hu, Junli Chang, Hongfeng Ruan, Wenlan Zhi, Xiaobo Wang, Fulai Zhao, Xiaoping Ma, Xingyuan Sun, Qianqian Liang, Hao Xu, Yongjun Wang, Yanping Yang
Summary: Down-regulated DKK3 indicates poor prognosis in osteosarcoma patients; up-regulated miR-214-3p promotes proliferation and migration of osteosarcoma cells by increasing β-catenin nuclear translocation and LEF1 translation via degradation of DKK3. Cantharidin inhibits osteosarcoma cell viability, migration, and invasion by down-regulating miR-214-3p to up-regulate DKK3, leading to decreased β-catenin nuclear translocation and LEF1 translation.
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
(2021)
Article
Gastroenterology & Hepatology
Yu Qi, Yujia Shan, Shuangda Li, Yiran Huang, Yanru Guo, Tong Huang, Xinyu Zhao, Li Jia
Summary: Our study identified higher levels of LEF1-AS1 and LEF1 in CRC tissues compared to adjacent tissues, as well as upregulation in CRC cell lines compared to normal colorectal cells. LEF1-AS1 recruits MLL1 to regulate LEF1 expression, while FUT8 is positively correlated to LEF1 and modulated by LEF1. The axis of LEF1-AS1/LEF1/FUT8 in CRC progression involves the Wnt/beta-catenin pathway and alpha 1,6-fucosylation, presenting a potential therapeutic target for CRC.
DIGESTIVE DISEASES AND SCIENCES
(2022)
Article
Oncology
Hongyan Li, Ning Zhang, Xueli Jiao, Cong Wang, Wenhao Sun, Yanyu He, Ganglin Ren, Shirui Huang, Mengjie Li, Yixin Chang, Zihui Jin, Qipeng Xie, Xiaodong Zhang, Haishan Huang, Honglei Jin
Summary: MiR-6125 is downregulated in CRC, negatively correlated with tumor size and prognosis. MiR-6125 targets YTHDF2, downregulates its protein, stabilizes m6A-modified GSK3 beta mRNA. Increased GSK3 beta protein inhibits Wnt/beta-catenin/Cyclin D1 pathway proteins, leading to G0-G1 phase arrest and inhibiting CRC cell proliferation.
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Jilly Frances Evans, Kseniya Obraztsova, Susan M. Lin, Vera P. Krymskaya
Summary: The study focuses on the mechanistic target of rapamycin (mTOR) and wingless-related integration site (Wnt) signal transduction networks and their impact on cancer, drug resistance, and stem cell exhaustion, as well as the lung disease lymphangioleiomyomatosis (LAM). The review highlights potential therapeutic approaches targeting these pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Geriatrics & Gerontology
Y. Li, W. Cui, B. Song, X. Ye, Z. Li, C. Lu
Summary: This study found that SIRT1 activator has a negative regulatory effect on weight, blood lipid levels, and lesion conditions in AS mice, and reduces the CD34+/VEGFR2+ density in aortic tissues. In in vitro experiments, the SIRT1 activator promotes the proliferation and migration of EPCs and acts through the wnt/beta-catenin/GSK3 beta signaling pathway.
JOURNAL OF NUTRITION HEALTH & AGING
(2022)
Article
Genetics & Heredity
Yibo Wang, Kai Hang, Li Ying, Jiaqi Wu, Xiaoyong Wu, Weijun Zhang, Lijun Li, Zhongxiang Wang, Jinwu Bai, Xiang Gao, Deting Xue, Zhijun Pan
Summary: Chaperone-mediated autophagy (CMA) protein LAMP2A plays a crucial role in regulating the adipo-osteogenesis process of MSCs. Co-expression analyses revealed the relationships between LAMP2A and other relevant genes. In vitro and in vivo experiments demonstrated that LAMP2A positively regulates mMSC osteogenesis, suppresses adipo-osteogenesis, and promotes fracture healing in a mouse model.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Biochemistry & Molecular Biology
Maria Abancens, Brian J. Harvey, Jean McBryan
Summary: Women have lower incidence and mortality rates for colorectal cancer compared to men. Estrogen may protect against colorectal cancer progression through GPER-mediated modulation of the Wnt/beta-catenin pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Anil Belur Nagaraj, Matthew Knarr, Sreeja Sekhar, R. Shae Connor, Peronne Joseph, Olga Kovalenko, Alexis Fleming, Arshia Surti, Elmar Nurmemmedov, Luca Beltrame, Sergio Marchini, Michael Kahn, Analisa DiFeo
Summary: Wnt signaling plays a critical role in ovarian cancer, and miR-181a has been identified as a novel driver of the Wnt/β-catenin signaling pathway, promoting stemness and chemoresistance. Targeting the miR-181a-SFRP4 axis may offer a potential therapeutic strategy for HGSOC.
Article
Pharmacology & Pharmacy
Young Yun Jung, Jae-Young Um, Arunachalam Chinnathambi, Chandramohan Govindasamy, Acharan S. Narula, Ojas A. Namjoshi, Bruce E. Blough, Gautam Sethi, Kwang Seok Ahn
Summary: This study investigated the effects of Withaferin A (WFA) on apoptosis and autophagy in human colorectal cancer cells. The results showed that WFA could induce cell cycle arrest, increase apoptotic cell death, and activate autophagy. The WFA-induced effects on apoptosis and autophagy were mediated through the modulation of the beta-catenin pathway.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Review
Clinical Neurology
Yutaka Mizuki, Shinji Sakamoto, Yuko Okahisa, Yuji Yada, Nozomu Hashimoto, Manabu Takaki, Norihito Yamada
Summary: Patients with schizophrenia have a high comorbidity of type 2 diabetes mellitus, likely due to antipsychotic-induced weight gain and cognitive dysfunction resulting in a disordered lifestyle. Common susceptibility genes may play a role in the association between schizophrenia and type 2 diabetes, regulating neuronal development in the brain and insulin in the pancreas through shared cascades. While genetic analyses have linked the two disorders, inconsistency in results and low to moderate risk associated with susceptibility genes suggest future research directions are needed.
INTERNATIONAL JOURNAL OF NEUROPSYCHOPHARMACOLOGY
(2021)
Article
Cell Biology
Vikash Singh, Vonn Walter, Irina Elcheva, Yuka Imamura Kawasawa, Vladimir S. Spiegelman
Summary: In this study, the role of IGF2BP1 in the post-transcriptional regulation of Wnt/beta-catenin-induced genes in colorectal cancer (CRC) cells was investigated using iCLIP and RNA sequencing techniques. The study revealed that CRC cells exhibit a stronger dependency on IGF2BP1 expression for Wnt/beta-catenin-regulated genes compared to normal cells. iCLIP analysis showed significant changes in IGF2BP1-binding sites and enrichment of 6-mer motifs associated with IGF2BP1 binding in response to Wnt/beta-catenin signaling. Additionally, a signature of IGF2BP1-regulated genes significantly associated with colon cancer-free survival in humans was identified.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Oncology
Anja Kafka, Anja Bukovac, Emilija Brglez, Ana-Marija Jarmek, Karolina Poljak, Petar Brlek, Kamelija Zarkovic, Niko Njiric, Nives Pecina-Slaus
Summary: This study investigated genetic and epigenetic changes as well as protein expression levels of negative regulators of Wnt signaling in human astrocytomas. The results emphasize the importance of methylation in Wnt signaling regulation, and suggest that large deletions of the APC gene and effects of beta-catenin and GSK3 beta play key roles in astrocytoma evolution. Further studies are needed to understand the clinical and therapeutic relevance of these molecular alterations.
Article
Biotechnology & Applied Microbiology
Yunpeng Sun, Pengfei Wang, Qiyu Zhang, Huanhuan Wu
Summary: This study revealed a positive feedback regulation involving CDK14, beta-catenin, TCF4, and miR-26b in modulating the phenotypes of pancreatic cancer cells in vitro and tumor growth in vivo.
JOURNAL OF GENE MEDICINE
(2022)
Article
Oncology
Ruijie Liu, Ping Deng, Yonglian Zhang, Yonglan Wang, Cuiping Peng
Summary: The study revealed that circ_0082182 is upregulated in CRC and promotes cell proliferation, cell cycle progression, and metastasis while inhibiting apoptosis by acting as a sponge for miR-411 and miR-1205. Additionally, circ_0082182 accelerates CRC progression by activating the Wnt/beta-catenin pathway and EMT process.
WORLD JOURNAL OF SURGICAL ONCOLOGY
(2021)