Article
Environmental Sciences
Mei Pan, Haizong Li, Xiangyun Han, Weixing Ma, Xuan Li, Qingyuan Guo, Bairen Yang, Cheng Ding, Yuwen Ma
Summary: This study investigates the effects of different hydrodynamic conditions on biofilm composition and architecture. The results show that the thickness and compactness of biofilms vary under different flow conditions. The distribution of extracellular polysaccharides, proteins, and bacteria also differs. Additionally, the study observes the secretion of different types of EPS and their role in biofilm formation.
Article
Immunology
Jiwasmika Baishya, Jake A. Everett, Walter J. Chazin, Kendra P. Rumbaugh, Catherine A. Wakeman
Summary: Calprotectin, a transition metal chelating protein, plays a role in nutritional immunity and has antimicrobial activity. It interacts with biofilms of Pseudomonas aeruginosa and Staphylococcus aureus, leading to compositional changes in the biofilm extracellular polymeric substance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Yiwen Zhu, Shijing Wu, Yujie Sun, Xuan Zou, Liang Zheng, Shun Duan, Julin Wang, Bingran Yu, Ruifang Sui, Fu-Jian Xu
Summary: The newly designed P alpha Gal(50)-b-PGRB(20) can effectively disperse biofilms and selectively kill MDR-P. aeruginosa within biofilms by binding to Lec A in the extracellular polymeric substances and on the P. aeruginosa membrane under low phototoxicity caused by broad-spectrum aPDT. It has bactericidal activities by damaging DNA, RNA, protein, and membrane, showing potential for better corneal recovery in MDR-P. aeruginosa biofilm infected keratitis model.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Nanoscience & Nanotechnology
Lei Xin, Chao Zhang, Jifan Chen, Yifan Jiang, Yajing Liu, Peile Jin, Xue Wang, Guowei Wang, Pintong Huang
Summary: This study presents the design of ultrasound-activatable targeted nanoparticles that can effectively eradicate biofilms, clear bacteria, and accelerate wound healing.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Microbiology
Ziwei Liang, Martin Nilsson, Kasper Norskov Kragh, Ida Hedal, Julia Alcacer-Almansa, Rikke Overgaard Kiilerich, Jens Bo Andersen, Tim Tolker-Nielsen
Summary: The bacterium Pseudomonas aeruginosa is involved in chronic infections, and the exopolysaccharides produced by this bacterium play a role in the persistence and antibiotic tolerance of the bacteria. In this study, the researchers investigated the individual roles of three exopolysaccharides in antibiotic tolerance. They found that alginate contributes to the tolerance of P. aeruginosa aggregates towards certain antibiotics, while Psl and Pel do not.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Eleonora Secchi, Giovanni Savorana, Alessandra Vitale, Leo Eberl, Roman Stocker, Roberto Rusconi
Summary: It has been found that the mechanical stability of Pseudomonas aeruginosa streamers mainly relies on the presence of extracellular DNA (eDNA) and the exopolysaccharide Pel. Controlling the composition of streamers can effectively control the formation of biofilm structures.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Environmental Sciences
Li Yin, Juan Wang, Kaipian Shi, Yong Zhang, Ying Xu, Desheng Kong, Lixiao Ni, Shiyin Li
Summary: This study explores the protective mechanism of extracellular polymeric substance (EPS) secreted by harmful cyanobacteria against tannins allelochemicals. The results show that proteins in soluble EPS (SEPS) and bound EPS (BEPS) of Microcystis aeruginosa interact with tannic acid (TA) mainly through hydrophobic interactions. The amine, carbonyl, carboxyl, and hydroxyl groups in EPS are the functional groups contributing to the interaction with TA. The existence of EPS reduces the toxicity of TA to algal cells.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Engineering, Environmental
Xiao-Yang Liu, Shuxia Guo, Thomas Bocklitz, Petra Roesch, Juergen Popp, Han-Qing Yu
Summary: This study developed an approach based on confocal Raman mapping and non-negative matrix factorization analysis to probe the hydrated biofilm matrix in situ. The method was able to resolve the chemical components of the biofilm and visualize their spatial organization. The study found diverse chemical components within different Pseudomonas spp. biofilm matrices and identified their relationship with specific physiological functions and microenvironments.
Article
Anatomy & Morphology
Kumari Uma Mahto, Surajit Das
Summary: Biofilm formation in bacteria is influenced by the nature of substrata. A marine bacterium Pseudomonas aeruginosa PFL-P1 formed dense biofilms with varied aggregation on different substrata, with ceramic substratum showing the highest roughness and maximum biofilm thickness. The study provides insight into how substratum properties can impact biofilm formation in bacteria.
MICROSCOPY RESEARCH AND TECHNIQUE
(2021)
Article
Pharmacology & Pharmacy
Sunil Kumar Bose, Karuna Sharma, Sanjay Chhibber, Kusum Harjai
Summary: The study demonstrated that the use of nanolipoidal alpha-terpineol could effectively treat Pseudomonas aeruginosa-induced keratitis, showing significant improvements in bacterial count, corneal pathology, and immunomodulatory effects.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Infectious Diseases
Taehyeong Lim, So-Young Ham, SangJin Nam, Myoungsun Kim, Ki Yong Lee, Hee-Deung Park, Youngjoo Byun
Summary: This article reviews recent studies on small molecules targeting the RhlI/RhlR system in P. aeruginosa, discussing their in vitro and in vivo biological activities. The article focuses on structure-activity relationship studies and the outlook for next-generation anti-biofilm agents.
Article
Biotechnology & Applied Microbiology
James Redfern, Janine Wallace, Alex van Belkum, Magali Jaillard, Elliot Whittard, Roobinidevi Ragupathy, Joanna Verran, Peter Kelly, Mark Charles Enright
Summary: Pseudomonas aeruginosa is a common environmental microorganism that can form biofilms on indwelling device surfaces, promoting adhesion and survival. Research identified accessory genes and core-genome SNPs associated with enhanced biofilm production at 22 degrees C, including a 165 kb genomic island with heavy metal resistance genes.
Article
Environmental Sciences
Mei Pan, Haizong Li, Xiangyun Han, Siyi Jiang, Yusen Diao, Weixing Ma, Xuan Li, Jiaojiao Qin, Jianchun Yao, Zhitong Wang
Summary: This study investigated the regulatory effects of different fractions of extracellular polymeric substances (EPS) on biofilm formation. It was found that tightly bound EPS (TB-EPS) and loosely bound EPS (LB-EPS) were secreted more in turbulent flow, while dissolved EPS (SB-EPS) was secreted slightly more in laminar flow. Polysaccharides, which enhance bacterial aggregation and make biofilms dense, were found to be the core of biofilm formation. The special bonding effects of LB-EPS and TB-EPS were also crucial in the formation of mature biofilms.
Article
Infectious Diseases
Tomasz Bogiel, Dagmara Depka, Mateusz Rzepka, Joanna Kwiecinska-Pirog, Eugenia Gospodarek-Komkowska
Summary: The study investigated the frequency and genotype distribution of 10 virulence factor genes in 107 non-duplicated carbapenem-resistant P. aeruginosa isolates. Variations were observed in the prevalence and distribution of virulence genes, especially in terms of their carbapenemase synthesis ability and the strain origin.
Article
Biology
Steffen Geisel, Eleonora Secchi, Jan Vermant
Summary: Biofilms are bacterial communities encased by a self-produced matrix, and the channel networks formed within the biofilm play a vital role in promoting the transport of nutrients and chemicals. This study focuses on the role of the biofilm matrix adhesion in channel formation, specifically in Pseudomonas aeruginosa biofilms grown under constant flow in microfluidic channels. The formation of wrinkles and folds is triggered by a mechanical buckling instability, resulting in hollow channels that facilitate bacterial movement.