From the Genome to the Phenome: Tools to Understand the Basic Biology ofPlasmodium falciparum

A Next-generation Genetically Attenuated Plasmodium falciparum Parasite Created by Triple Gene Deletion

(2014) Sebastian A Mikolajczak et al.   MOLECULAR THERAPY

PTEX is an essential nexus for protein export in malaria parasites

(2014) Brendan Elsworth et al.   NATURE

PTEX component HSP101 mediates export of diverse malaria effectors into host erythrocytes

(2014) Josh R. Beck et al.   NATURE

Genome editing in the human malaria parasite Plasmodium falciparum using the CRISPR-Cas9 system

(2014) Mehdi Ghorbal et al.   NATURE BIOTECHNOLOGY

Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum

(2014) Jeffrey C Wagner et al.   NATURE METHODS

Na+ Regulation in the Malaria Parasite Plasmodium falciparum Involves the Cation ATPase PfATP4 and Is a Target of the Spiroindolone Antimalarials

(2013) Natalie J. Spillman et al.   Cell Host & Microbe

Spatial and temporal mapping of the PfEMP1 export pathway inPlasmodium falciparum

(2013) Paul J. McMillan et al.   CELLULAR MICROBIOLOGY

Perturbations ofPlasmodium Puf2 expression and RNA-seq of Puf2-deficient sporozoites reveal a critical role in maintaining RNA homeostasis and parasite transmissibility

(2013) Scott E. Lindner et al.   CELLULAR MICROBIOLOGY

Type II Fatty Acid Biosynthesis Is Essential for Plasmodium falciparum Sporozoite Development in the Midgut of Anopheles Mosquitoes

(2013) Ben C. L. van Schaijk et al.   EUKARYOTIC CELL

Malaria parasites tolerate a broad range of ionic environments and do not require host cation remodelling

(2013) Ajay D. Pillai et al.   MOLECULAR MICROBIOLOGY

Robust inducible Cre recombinase activity in the human malaria parasitePlasmodium falciparumenables efficient gene deletion within a single asexual erythrocytic growth cycle

(2013) Christine R. Collins et al.   MOLECULAR MICROBIOLOGY

Targeting Plasmodium PI(4)K to eliminate malaria

(2013) Case W. McNamara et al.   NATURE

A molecular marker of artemisinin-resistant Plasmodium falciparum malaria

(2013) Frédéric Ariey et al.   NATURE

Malaria parasites utilize both homologous recombination and alternative end joining pathways to maintain genome integrity

(2013) Laura A. Kirkman et al.   NUCLEIC ACIDS RESEARCH

Inducible Knockdown of Plasmodium Gene Expression Using the glmS Ribozyme

(2013) Parichat Prommana et al.   PLoS One

2A and the Auxin-Based Degron System Facilitate Control of Protein Levels in Plasmodium falciparum

(2013) Andrea Kreidenweiss et al.   PLoS One

Transposon mutagenesis identifies genes essential for Plasmodium falciparum gametocytogenesis

(2013) H. Ikadai et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spatial association with PTEX complexes defines regions for effector export into Plasmodium falciparum-infected erythrocytes

(2013) David T. Riglar et al.   Nature Communications

Novel Nanosomes for Gene Delivery to Plasmodium falciparum-infected Red Blood Cells

(2013) Anusha M. Gopalakrishnan et al.   Scientific Reports

A Tetracycline-Repressible Transactivator System to Study Essential Genes in Malaria Parasites

(2012) Paco Pino et al.   Cell Host & Microbe

Analysis of Protein Palmitoylation Reveals a Pervasive Role in Plasmodium Development and Pathogenesis

(2012) Matthew L. Jones et al.   Cell Host & Microbe

Plasmodium Drug Targets Outside the Genetic Control of the Parasite

(2012) David J. Sullivan   CURRENT PHARMACEUTICAL DESIGN

Biosynthesis, Localization, and Macromolecular Arrangement of thePlasmodium falciparumTranslocon of Exported Proteins (PTEX)

(2012) Hayley E. Bullen et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

ATP and luciferase assays to determine the rate of drug action in in vitro cultures of Plasmodium falciparum

(2012) Tasmiyah Khan et al.   MALARIA JOURNAL

Plate-based transfection and culturing technique for genetic manipulation of Plasmodium falciparum

(2012) Florence Caro et al.   MALARIA JOURNAL

The Plasmodium falciparum, Nima-related kinase Pfnek-4: a marker for asexual parasites committed to sexual differentiation

(2012) Luc Reininger et al.   MALARIA JOURNAL

A high-throughput method to detect Plasmodium falciparum clones in limiting dilution microplates

(2012) Brian Lyko et al.   MALARIA JOURNAL

Comparison of the absolute and relative efficiencies of electroporation-based transfection protocols for Plasmodium falciparum

(2012) Sandra Hasenkamp et al.   MALARIA JOURNAL

Wherever I may roam: Protein and membrane trafficking in P. falciparum-infected red blood cells

(2012) Marcel Deponte et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Conditional genome engineering in Toxoplasma gondii uncovers alternative invasion mechanisms

(2012) Nicole Andenmatten et al.   NATURE METHODS

Site-specific genome editing in Plasmodium falciparum using engineered zinc-finger nucleases

(2012) Judith Straimer et al.   NATURE METHODS

The role of N-terminus of Plasmodium falciparum ORC1 in telomeric localization and var gene silencing

(2012) Abhijit S. Deshmukh et al.   NUCLEIC ACIDS RESEARCH

Investigation of the Plasmodium falciparum Food Vacuole through Inducible Expression of the Chloroquine Resistance Transporter (PfCRT)

(2012) Florian Ehlgen et al.   PLoS One

Systematic Analysis of FKBP Inducible Degradation Domain Tagging Strategies for the Human Malaria Parasite Plasmodium falciparum

(2012) Mauro Ferreira de Azevedo et al.   PLoS One

Determination of protein subcellular localization in apicomplexan parasites

(2012) Ben J. Woodcroft et al.   TRENDS IN PARASITOLOGY

PfCRT and its role in antimalarial drug resistance

(2012) Andrea Ecker et al.   TRENDS IN PARASITOLOGY

Plasmodium falciparum  heat shock protein 110 stabilizes the asparagine repeat-rich parasite proteome during malarial fevers

(2012) Vasant Muralidharan et al.   Nature Communications

CCR4-Associated Factor 1 Coordinates the Expression of Plasmodium falciparum Egress and Invasion Proteins

(2011) Bharath Balu et al.   EUKARYOTIC CELL

ATP Synthase Complex ofPlasmodium falciparum

(2011) Praveen Balabaskaran Nina et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Analysis of the Molecular Mechanisms Governing the Stage-Specific Expression of a Prototypical Housekeeping Gene during Intraerythrocytic Development of P. falciparum

(2011) Eleanor H. Wong et al.   JOURNAL OF MOLECULAR BIOLOGY

RNAi knock-downs support roles for the mucin-like (AeIMUC1) gene and short-chain dehydrogenase/reductase (SDR) gene in Aedes aegypti susceptibility to Plasmodium gallinaceum

(2011) M. BEROIS et al.   MEDICAL AND VETERINARY ENTOMOLOGY

Malaria drug resistance is associated with defective DNA mismatch repair

(2011) Meryl A. Castellini et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodium falciparum transfection

(2011) Suresh M. Ganesan et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Asparagine repeat function in a Plasmodium falciparum protein assessed via a regulatable fluorescent affinity tag

(2011) V. Muralidharan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Global kinomic and phospho-proteomic analyses of the human malaria parasite Plasmodium falciparum

(2011) Lev Solyakov et al.   Nature Communications

Chemical Rescue of Malaria Parasites Lacking an Apicoplast Defines Organelle Function in Blood-Stage Plasmodium falciparum

(2011) Ellen Yeh et al.   PLOS BIOLOGY

Identification and Functional Validation of the Novel Antimalarial Resistance Locus PF10_0355 in Plasmodium falciparum

(2011) Daria Van Tyne et al.   PLoS Genetics

Engineering a Direct and Inducible Protein−RNA Interaction To Regulate RNA Biology

(2010) Brian J. Belmont et al.   ACS Chemical Biology

Cloning of Plasmodium falciparum by single-cell sorting

(2010) Jun Miao et al.   EXPERIMENTAL PARASITOLOGY

Gene deletion from Plasmodium falciparum using FLP and Cre recombinases: Implications for applied site-specific recombination

(2010) Matthew T. O’Neill et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Development of fluorescent Plasmodium falciparum for in vitro growth inhibition assays

(2010) Danny W Wilson et al.   MALARIA JOURNAL

Validation of a modified method for Bxb1 mycobacteriophage integrase-mediated recombination in Plasmodium falciparum by localization of the H-protein of the glycine cleavage complex to the mitochondrion

(2010) Maroya D. Spalding et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Plasmodiumpyruvate dehydrogenase activity is only essential for the parasite's progression from liver infection to blood infection

(2010) Ying Pei et al.   MOLECULAR MICROBIOLOGY

Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions

(2010) Anita A Koshy et al.   NATURE METHODS

Removal of Heterologous Sequences from Plasmodium falciparum Mutants Using FLPe-Recombinase

(2010) Ben C. L. van Schaijk et al.   PLoS One

A Genetic Screen for Attenuated Growth Identifies Genes Crucial for Intraerythrocytic Development of Plasmodium falciparum

(2010) Bharath Balu et al.   PLoS One

A Plant-Like Kinase in Plasmodium falciparum Regulates Parasite Egress from Erythrocytes

(2010) J. D. Dvorin et al.   SCIENCE

piggyBac is an effective tool for functional analysis of the Plasmodium falciparum genome

(2009) Bharath Balu et al.   BMC MICROBIOLOGY

Clonal Conditional Mutagenesis in Malaria Parasites

(2009) Audrey Combe et al.   Cell Host & Microbe

An RNA Aptamer that Induces Transcription

(2009) Anke Hunsicker et al.   CHEMISTRY & BIOLOGY

A newly discovered protein export machine in malaria parasites

(2009) Tania F. de Koning-Ward et al.   NATURE

Molecular genetics and comparative genomics reveal RNAi is not functional in malaria parasites

(2009) Jake Baum et al.   NUCLEIC ACIDS RESEARCH

In vitro selection of RNA aptamers against a conserved region of the Plasmodium falciparum erythrocyte membrane protein 1

(2009) Anders Barfod et al.   PARASITOLOGY RESEARCH

Inhibiting Plasmodium falciparum growth and heme detoxification pathway using heme-binding DNA aptamers

(2009) J. C. Niles et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A calpain unique to alveolates is essential in Plasmodium falciparum and its knockdown reveals an involvement in pre-S-phase development

(2009) I. Russo et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Plasmodium falciparum biology: analysis of in vitro versus in vivo growth conditions

(2009) Michele LeRoux et al.   TRENDS IN PARASITOLOGY

Recent progress with FKBP-derived destabilizing domains

(2008) Bernard W. Chu et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Type II fatty acid synthesis is essential only for malaria parasite late liver stage development

(2008) Ashley M. Vaughan et al.   CELLULAR MICROBIOLOGY

A regulatable transgene expression system for cultured Plasmodium falciparum parasites

(2008) Christian Epp et al.   MALARIA JOURNAL

Genetic evidence for the essential role of PfNT1 in the transport and utilization of xanthine, guanine, guanosine and adenine by Plasmodium falciparum

(2008) Kamal El Bissati et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY

Ribozyme cleavage of Plasmodium falciparum gyrase A gene transcript affects the parasite growth

(2008) Anwar Ahmed et al.   PARASITOLOGY RESEARCH

Evolution of malaria parasite plastid targeting sequences

(2008) C. J. Tonkin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In silicoactivity profiling reveals the mechanism of action of antimalarials discovered in a high-throughput screen

(2008) David Plouffe et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Kinome-Wide RNAi Screen Implicates at Least 5 Host Hepatocyte Kinases in Plasmodium Sporozoite Infection

(2008) Miguel Prudêncio et al.   PLoS Pathogens

Functional expression of ribozymes in Apicomplexa: Towards exogenous control of gene expression by inducible RNA-cleavage

(2007) Carolina Agop-Nersesian et al.   INTERNATIONAL JOURNAL FOR PARASITOLOGY

Plasmodium falciparum signal peptidase is regulated by phosphorylation and required for intra-erythrocytic growth

(2007) Renu Tuteja et al.   MOLECULAR AND BIOCHEMICAL PARASITOLOGY