Journal
JOURNAL OF EUKARYOTIC MICROBIOLOGY
Volume 60, Issue 1, Pages 101-105Publisher
WILEY-BLACKWELL
DOI: 10.1111/jeu.12009
Keywords
MORN domain; parvulin; peptidyl-prolyl isomerase; Pin1; PPIase; Trypanosoma cruzi
Categories
Funding
- Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET, Argentina)
- Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT, Argentina)
- Prosul-CNPq
- FAPESP (Brazil)
Ask authors/readers for more resources
Pin1-type peptidyl-prolyl cis/trans isomerases (PPIases) isomerise the peptide bond of specific phosphorylated (Ser/Thr)-Pro residues, regulating various cellular events. Previously, we reported a Pin1-type PPIase in Trypanosoma cruzi, but little is known about its function and subcellular localization. Immunofluorescence analysis revealed that in contrast with Pin1-like proteins from diverse organisms, TcPin1 mainly localized in the cytoplasm and was excluded from the nuclei. In addition, RNAi-mediated downregulation of TbPin1 in Trypanosoma brucei did not abolish cell proliferation. Using yeast two-hybrid assay, we identified a MORN domain-containing protein as putative Pin1-binding partners. These data suggest that Pin1-mediated signaling mechanism plays a different role in protozoan parasites.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available