Essential oils composition of four Piper species from Brazil

The Piper L. genus has a great variety of commercially, pharmacologically and biologically interesting species. The present study focused on essential oils extracted by means of the hydrodistillation from dry leaves of Piper cernuum, Piper glabratum, Piper hispidum and Piper madeiranum. The species supplied essential oil contents of between 0.5% and 0.8%. Gas chromatography-mass spectrometry analyses revealed that the majority of components were -elemene (11.6%) and epi-cubebol (13.1%) for P. cernuum; -caryophyllene (14.6%) and longiborneol (12.0%) for P. glabratum; -pinene (12.0%), khusimene (12.1%) and -cadinene (13.2%) for P. hispidum;-caryophyllene (11.2%) and germacrene D-4-ol (11.1%) for P. madeiranum. The test carried out on the essential oils concerning the Artemia salina L. produced the following values for LC50 200.03 g/mL (P. cernuum), 45.21 g/mL (P. glabratum), 404.80 g/mL (P. hispidum) and 49.64 g/mL (P. madeiranum).