4.2 Article

Bacterial diversity in Fez tanneries and Morocco's Binlamdoune River, using 16S RNA gene based fingerprinting

Journal

JOURNAL OF ENVIRONMENTAL SCIENCES
Volume 22, Issue 12, Pages 1944-1953

Publisher

SCIENCE PRESS
DOI: 10.1016/S1001-0742(09)60344-2

Keywords

Fez tanneries; Binlamdoune River; biodiversity; bacteria; 16S rRNA

Funding

  1. Interuniversity Cooperation Program and Scientific Research [A/5712/06]

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Tannery wastewater causes serious ecological and sanitary damage Chemical analysis of water from Binlamdoune River of the medina of Fez was conducted and the results revealed the presence of toxic elements from tanneries and other industrial activities which strongly affected water quality To determine the effectiveness of bioremediation for depollution, we studied the abundance and diversity of bacteria residing in these polluted environments Conducting denaturing gradient gel electrophoresis (PCR-DGGE) of the 16S rDNA area using pruners related to bacteria showed a bacterial community belonging to eubacterial groups, that is Epsilonproteobacteria Clostridia Lactobacillales, Bacteroidetes Gammaproteobacteria, and Alphaproteobacteria In addition cloning displayed the presence of clones belonging to the Firmicutes group Moreover scanning electron microscopy revealed a significant heterogeneity of microorganism forms and structures These endogenous microbes could have a significant role in the purification of Binlamdoune River and Fez tannery wastewater

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