4.0 Article

Testing procedures for the determination of several biomarkers in different species, for environmental assessment of pollution

Journal

JOURNAL OF ENVIRONMENTAL MONITORING
Volume 12, Issue 8, Pages 1625-1630

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/b926647j

Keywords

-

Funding

  1. Fundacao para a Ciencia e a Tecnologia [PTDC/AMB/70431/2006, POCI/AMB/60899/2004]
  2. [SFRH/BPD/40052/2007]
  3. [SFRH/BD/38282/2007]
  4. Fundação para a Ciência e a Tecnologia [SFRH/BD/38282/2007, PTDC/AMB/70431/2006, POCI/AMB/60899/2004] Funding Source: FCT

Ask authors/readers for more resources

Antioxidant defences are the primary mechanisms by which organisms compensate oxidative damage caused by contact with several types of substances prone to establish oxidative cycles. Biomarkers are one of the most useful tools to assess effects consequent to environmental exposure to a large number of xenobiotics. However, the use of biomarkers requires previous standardization of enzymatic protocols in order to guarantee accuracy and relevance of obtained results. This article describes standardized conditions for the use of several enzymatic biomarkers (oxidative stress: glutathione peroxidase, lipid peroxidation, glutathione reductase, glutathione-S-transferase and catalase; respiration: lactate dehydrogenase) for the evaluation of responses of specimens of Eisenia andrei, Pelophylax perezi (formerly Rana perezi) and Apodemus sylvaticus. Furthermore, we also present the normal values of activity for the given markers, for non-exposed organisms, that can serve as comparative indexes for subsequent studies. We conclude that these species are thus a suitable target to serve as sentinels in studies of environmental contamination by chemicals, which can lead to oxidative and physiological modifications.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.0
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

Article Ecology

Recommended rates of azoxystrobin and tebuconazole seem to be environmentally safe but ineffective against target fungi

Libania Queiros, Nuno Aguiar, Patricia Pereira, Fernando J. M. Goncalves, Artur Alves, Joana Luisa Pereira

Summary: This study investigates the possibility of readjusting the formulation of a commercial fungicide to make it more environmentally safe and effective against target organisms. The results suggest that the current mixture and rates of the fungicides are generally environmentally safe but ineffective against the target fungi. It also highlights the importance of systematic ecotoxicological testing for optimizing commercial products and reducing environmental contamination.

ECOTOXICOLOGY (2023)

Article Marine & Freshwater Biology

Macroinvertebrate community structure and ecological status in Portuguese streams across climatic and water scarcity gradients

Joana Isabel Santos, Carlos Silva, Fernando J. M. Goncalves, Joana Luisa Pereira, Bruno Branco Castro

Summary: The aim of this study was to compare the macroinvertebrate community structure and its bioindicator value in streams from different regions in Portugal. The results showed clear differences in environmental context and ecological similarity between the wettest and driest regions, while the intermediate region had high variability. Climate and water scarcity were found to be coupled with other environmental drivers in shaping macroinvertebrate communities.

HYDROBIOLOGIA (2023)

Article Biotechnology & Applied Microbiology

Are cyanobacteria a nearly immortal source of high market value compounds?

Ines P. E. Macario, Telma Veloso, Andreia P. M. Fernandes, Margarida Martins, Silja Frankenbach, Joao Serodio, Fernando J. M. Goncalves, Sonia P. M. Ventura, Joana L. Pereira

Summary: This study evaluated the growth patterns and pigment production of three cyanobacterium species, and found that N. muscorum was the best producer of chlorophyll a and C-phycocyanin.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2023)

Article Engineering, Environmental

Ecotoxicological relevance of glyphosate and flazasulfuron to soil habitat and retention functions - Single vs combined exposures

Cristiano Soares, Beatriz Fernandes, Cristiana Paiva, Veronica Nogueira, Anabela Cachada, Fernanda Fidalgo, Ruth Pereira

Summary: Glyphosate (GLY) and flazasulfuron (FLA) have different effects on soil habitat and non-target organisms. FLA has more toxic effects on earthworms and plants, while GLY mainly affects plant growth and has no effect on collembola.

JOURNAL OF HAZARDOUS MATERIALS (2023)

Article Horticulture

Modulation of the non-target phytotoxicity of glyphosate by soil organic matter in tomato (Solanum lycopersicum L.) plants

Cristiano Soares, Pedro Mateus, Fernanda Fidalgo, Ruth Pereira

Summary: This study aimed to investigate the role of soil organic matter (OM) in preventing glyphosate (GLY) phytotoxicity on tomato plants. The results suggest that soils with higher OM content (10% and 15%) can mitigate the non-target phytotoxicity of GLY, possibly by decreasing herbicide bioavailability and/or stimulating defense mechanisms, thereby improving crop growth and physiological performance.

SCIENTIA HORTICULTURAE (2023)

Article Environmental Sciences

Do Freshwater and Marine Bivalves Differ in Their Response to Wildfire Ash? Effects on the Antioxidant Defense System and Metal Body Burden

Fatima Jesus, Filipa Mesquita, Elisa Virumbrales Aldama, Ana Marques, Ana M. M. Goncalves, Luisa Magalhaes, Antonio J. A. Nogueira, Ana Re, Isabel Campos, Joana Luisa Pereira, Fernando J. M. Goncalves, Nelson Abrantes, Dalila Serpa

Summary: This study compared the antioxidant defense response of the freshwater clam Corbicula fluminea and the marine cockle Cerastoderma edule to wildfire ash exposure and metal body burden. Both species exhibited significant changes in certain parameters after exposure to aqueous extracts of Eucalypt ash, although the effects differed between the species. Clams showed higher Cd content while cockles showed higher Cu content, indicating different responses to wildfire ash exposure.

INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH (2023)

Article Environmental Sciences

Intermittent Rivers as a Challenge for Freshwater Ecosystems Quality Evaluation: A Study Case in the Ribeira de Silveirinhos, Portugal

Sara Rodrigues, Barbara Xavier, Sandra Nogueira, Sara C. Antunes

Summary: This study assessed the ecological status of Ribeira de Silveirinhos in northern Portugal and found that anthropogenic pressures and climate change have led to changes in the hydrological connectivity and ecosystem functions of the river. The upstream areas, surrounded by Eucalyptus monoculture during drought periods, showed low pH values, while the downstream areas subjected to anthropogenic activities, iron waters, and agriculture had high concentrations of phosphorus. The macroinvertebrate community was also affected by the intermittency of flow. The results indicated that Ribeira de Silveirinhos had a poor to moderate ecological status. However, the protection of intermittent rivers and streams is often overlooked, highlighting the need to consider their specific ecological functioning and adapt planning and management models to ensure ecological quality and conservation processes.

WATER (2023)

Article Chemistry, Multidisciplinary

Preparation, Characterization, and Environmental Safety Assessment of Dithiocarbazate Loaded Mesoporous Silica Nanoparticles

Thacilla Menezes, Sirine Bouguerra, Tatiana Andreani, Ruth Pereira, Carlos Pereira

Summary: The main goal of this study was to design and evaluate the success of loading 3-methyl-5-phenyl-pyrazoline-1-(S-benzyldithiocarbazate) (DTC) into mesoporous silica nanoparticles (MSiNPs) by measuring its physicochemical properties and assessing the environmental safety of the new formulation using different aquatic organisms. The results showed that DTC, MSiNP, and MSiNP-DTC had low toxicity against aquatic organisms, but loading DTC slightly increased toxicity. Therefore, it is crucial to assess the impact of MSiNPs on the safety of the new formulation.

NANOMATERIALS (2023)

Article Marine & Freshwater Biology

A forecast effects of climate change and anthropogenic compounds in Gambusia holbrooki: ecotoxicological effects of salinity and metformin

Maria Sousa, Sara Rodrigues, Carlo Pretti, Valentina Meucci, Federica Battaglia, Rosa Freitas, Sara C. Antunes

Summary: Estuarine and coastal ecosystems are experiencing fluctuations in salinity due to global warming and extreme weather events, as well as increased water pollution from organic and inorganic compounds. This study investigated the acute and chronic ecotoxicological effects of metformin, an antidiabetic drug, on Gambusia holbrooki under different salinities. The results showed interactions between salinity and metformin, with increased oxidative stress and altered antioxidant capacity, protein content, and glycogen levels. These findings highlight the potential impact of metformin on aquatic organisms and the importance of considering multiple stressors in ecological risk assessments.

AQUATIC TOXICOLOGY (2023)

Article Environmental Sciences

Are biopesticides safe for the environment? Effects of pyrethrum extract on the non-target species Daphnia magna

B. S. Diogo, S. C. Antunes, S. Rodrigues

Summary: Biopesticides, such as pyrethrum extract (PE), are considered safer and more sustainable for the environment. However, this study found that PE can have acute and sub-chronic toxic effects on Daphnia magna, a non-target organism. The research demonstrated increased oxidative stress and lipid peroxidation in D. magna exposed to PE, indicating an imbalance in their oxidative metabolism.

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2023)

Article Environmental Sciences

Are the ecotoxicological tools viable to evaluate the effectiveness of wastewater treatment plant effluents?

B. S. Diogo, S. Rodrigues, O. M. Lage, S. C. Antunes

Summary: This study assessed the effectiveness of different treatments used in wastewater treatment plants (WWTP) using bioassays and biomarkers. The results showed that the tested species exhibited different sensitivities to the collected samples, and the assays using D. magna and L. minor demonstrated their potential for monitoring and assessing the environmental impact of wastewater effluents.

INTERNATIONAL JOURNAL OF ENVIRONMENTAL SCIENCE AND TECHNOLOGY (2023)

Article Environmental Sciences

Ecotoxicological and biochemical effects of a binary mixture of pesticides on the marine diatom Thalassiosira weissflogii in a scenario of global warming

Andreia F. Mesquita, Fatima Jesus, Fernando J. M. Goncalves, Ana M. M. Goncalves

Summary: Under the current scenario of global warming, this study aims to understand the effect of increased temperature on the combined toxicity of pesticides to aquatic species. The results showed that increased temperature increased the tolerance of diatoms to pesticides and also altered the biochemical responses of the diatoms. These findings have implications for the nutritional value of food webs.

SCIENCE OF THE TOTAL ENVIRONMENT (2023)

Article Environmental Sciences

Can Zooplankton Add Value to Monitoring Water Quality? A Case Study of a Meso/Eutrophic Portuguese Reservoir

Ivo Pinto, Sandra Nogueira, Sara Rodrigues, Nuno Formigo, Sara C. C. Antunes

Summary: This study revealed the importance of zooplankton as a bioindicator for assessing water quality in reservoirs. The current monitoring criteria are insufficient for a comprehensive evaluation of ecological status. By analyzing the zooplankton community, it is possible to accurately determine the water quality and different metrics provide valuable information for assessing water quality status.

WATER (2023)

Editorial Material Construction & Building Technology

Preface to the special issue: Urban food production: challenges and opportunities towards sustainable cities

C. S. C. Calheiros, R. Pereira, S. L. G. Skar, S. I. A. Pereira

SUSTAINABLE CITIES AND SOCIETY (2023)

Article Biochemistry & Molecular Biology

Effects of Inorganic and Organic Pollutants on the Biomarkers' Response of Cerastoderma edule under Temperature Scenarios

Andreia F. Mesquita, Fernando J. M. Goncalves, Ana M. M. Goncalves

Summary: Currently, there is a growing concern over chemical pollution and climate change worldwide. This study focuses on evaluating the effects of copper and oxyfluorfen on bivalve species, Cerastoderma edule, considering their tolerance and the changes in antioxidant defense enzymes under different temperatures. The results show that C. edule has higher tolerance to oxyfluorfen compared to copper. The antioxidant defense system effectively fights against the chemicals, but there may be neurotoxic effects at certain temperatures. Higher temperatures can lead to lipid peroxidation and increased mortality.

ANTIOXIDANTS (2023)

No Data Available