Article
Engineering, Biomedical
Zain Siddiqui, Biplab Sarkar, Ka-Kyung Kim, Nurten Kadincesme, Reshma Paul, Arjun Kumar, Yoshifumi Kobayashi, Abhishek Roy, Marwa Choudhury, Jian Yang, Emi Shimizu, Vivek A. Kumar
Summary: The study demonstrates the potential of acellular self-assembling peptide hydrogels in promoting tissue revascularization and soft tissue regeneration after endodontic treatment. The hydrogels show promise in guiding the development of neovasculature and tissue deposition, providing a scaffold for soft tissue regeneration in vivo.
ACTA BIOMATERIALIA
(2021)
Article
Dentistry, Oral Surgery & Medicine
Zafer C. Cehreli, Gizem Erbas Unverdi, Elif Ballikaya
Summary: This case series demonstrates the clinical and radiographic outcomes of regenerative endodontic treatment (RET) using deciduous pulp autotransplantation in traumatized, necrotic young permanent incisors. Follow-up observations at 12 and 24 months showed radiographic evidence of complete periapical healing, slight increase in dentinal wall thickness, and continued apical closure in all patients.
JOURNAL OF ENDODONTICS
(2022)
Article
Engineering, Biomedical
Qian Li, Zhiai Hu, Yongxi Liang, Cancan Xu, Yi Hong, Xiaohua Liu
Summary: The study developed a multifunctional peptide-conjugated, pH-sensitive non-viral gene vector for fast revascularization and pulp regeneration in a full-length human tooth root. This vector significantly increased gene transfection efficiency, reduced cytotoxicity, upregulated VEGF expression in dental pulp stem cells, and accelerated new blood vessel formation and pulp tissue regeneration. The approach proves to be safe and effective for regenerative endodontics.
ACTA BIOMATERIALIA
(2021)
Review
Pharmacology & Pharmacy
Hongbin Lai, Jiaqi Li, Xiaoxing Kou, Xueli Mao, Wei Zhao, Lan Ma
Summary: Extracellular vesicles (EVs) are lipid bound particles derived from their original cells, which play critical roles in intercellular communication through their cargoes. They can be divided into three categories: apoptotic vesicles (ApoVs), microvesicles (MVs), and small EVs (sEVs). Recently, the role of EVs in oral disease has received close attention. This review provides valuable insights into the potential therapeutic strategies of EVs in dental pulp and periodontal regeneration.
Article
Biotechnology & Applied Microbiology
Jiahui Fu, Jianfeng Chen, Wenjun Li, Xiaomin Yang, Jingyan Yang, Huixin Quan, Haitao Huang, Gang Chen
Summary: The supplementation of laminins to DPEM enhances odontogenic differentiation of cells and contributes to the formation of the odontoblast layer in dental pulp regeneration.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Yan Chen, Yue Ma, Xueting Yang, Jinlong Chen, Bo Yang, Weidong Tian
Summary: This study demonstrated that exosomes derived from dental pulp tissue can recruit stem cells to regenerate dental pulp, suggesting their potential in treating pulp deficiencies caused by various diseases.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Review
Cell Biology
Kyu Hwan Kwack, Hyeon-Woo Lee
Summary: Dental caries is a common disease that causes damage to teeth and pulp tissue. Dental pulp stem cells have multipotency, high proliferation rate, and immunosuppressive properties, making them ideal for regenerating damaged pulp tissue.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Pharmacology & Pharmacy
Nan Hu, Weiping Li, Wentao Jiang, Jin Wen, Shensheng Gu
Summary: Dental pulp and periapical diseases cause pain and economic loss for patients. Although root canal therapies provide relief, restoring the nutrition, sensory, and immune functions of dental pulp remains difficult. Tissue engineering and bioactive scaffolds have made significant progress in creating a suitable microenvironment for dental pulp regeneration. This article discusses current challenges and novel perspectives in using bioactive scaffolds to promote dental pulp regeneration.
Article
Cell & Tissue Engineering
Mohammed Zayed, Koichiro Iohara, Hideto Watanabe, Mami Ishikawa, Michiyo Tominaga, Misako Nakashima
Summary: The study found that hypoxia-preconditioned DPSCs (hpDPSCs) have advantages in proliferation rate and stemness, making them a potential effective source for pulp regeneration.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Physiology
Xiaoyao Huang, Zihan Li, Anqi Liu, Xuemei Liu, Hao Guo, Meiling Wu, Xiaoxue Yang, Bing Han, Kun Xuan
Summary: Dental pulp regeneration is crucial for restoring damaged dental pulp and teeth, with odontogenic mesenchymal stem cells playing a key role. The regenerative abilities of these cells are easily influenced by the surrounding microenvironment.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Immunology
Fenyao Li, Xinxin Wang, Jin Shi, Shuting Wu, Wenbo Xing, Yan He
Summary: Dental pulp stem cells have strong immunomodulatory capabilities and can be used to treat inflammation-related diseases and autoimmune disorders. The mechanism of action is complex and may involve the regulation of immune cells through inflammatory immune-related signaling pathways.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Maria Luisa Leite, Diana Gabriela Soares, Giovana Anovazzi, Caroline Anselmi, Josimeri Hebling, Carlos Alberto de Souza Costa
Summary: By varying the ratio of collagen and gelatin and incorporating different concentrations of fibronectin, the study found that collagen/gelatin hydrogel with 10 mg/mL of FN exhibited potent bioactive and chemotactic effects on human apical papilla cells.
JOURNAL OF ENDODONTICS
(2021)
Article
Engineering, Biomedical
Liwen Zheng, Yaxian Liu, Lin Jiang, Xiaoping Wang, Yuqin Chen, Lan Li, Mingyu Song, Hongmei Zhang, Yu Shrike Zhang, Ximu Zhang
Summary: This study proposes a new strategy for pulp regeneration by using hydrogel microspheres incorporated with decellularized dental pulp matrix-derived bioactive factors to simulate a pulp-specific three-dimensional microenvironment. The constructed microspheres exhibit favorable plasticity, biocompatibility, and biological performances, and hDPSCs cultured on the microspheres demonstrate enhanced pulp-formation ability. In vivo experiments show that the microspheres facilitate the regeneration of pulp-like tissue and new dentin. The decellularized pulp matrix-derived bioactive factors mediate the multi-directional differentiation of hDPSCs to regenerate the pulp tissue.
ACTA BIOMATERIALIA
(2023)
Review
Cell Biology
Wei Xiong, Ye Liu, Heng Zhou, Shuili Jing, Yan He, Qingsong Ye
Summary: Alzheimer's disease is a destructive neurodegenerative disease with a lack of effective drug candidates. Dental pulp stem cells have the potential for neuronal differentiation and neuroprotection, making them a promising approach for the treatment and protection of damaged neurons.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Jie Chen, Huaxing Xu, Kun Xia, Shuhua Cheng, Qi Zhang
Summary: In this study, it was found that RvE1 promotes pulp inflammation resolution and dentin regeneration, and positively influences the proliferation, chemotaxis, and differentiation of LPS-stimulated hDPSCs. The effects of RvE1 on DPSCs were mediated at least partially through AKT, ERK, and rS6-associated signaling in the inflammatory microenvironment. RvE1 shows promising application potential in regenerative endodontics.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Pharmacology & Pharmacy
Nupur Khera, Chafik Ghayor, Ekaterina Pavlova, Nina Atanassova, Franz E. Weber
Summary: N-methyl pyrrolidone (NMP) exhibits reproductive toxicity in male rats by causing testicular atrophy, DNA fragmentation in germ cells, and reduction in spermatocytes and spermatids. Its mechanism of action may involve low affinity inhibition of BRDT and decrease in testosterone levels. Monitoring the use of NMP in humans is crucial due to its interference with spermatogenesis at multiple levels.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Chafik Ghayor, Indranil Bhattacharya, Franz E. Weber
Summary: The study evaluated the impact of different pore-based microarchitectures on vertical bone augmentation and osteoconduction. Results showed that increasing pore diameter was positively correlated with the extent of vertical bone augmentation, with microarchitecture having a more significant impact on osteoconduction.
MATERIALS & DESIGN
(2021)
Review
Dentistry, Oral Surgery & Medicine
Matthias Zehnder, Georgios N. Belibasakis
Summary: This narrative review discusses the use of biomarkers in clinical Endodontic research, highlighting the importance of addressing clinical problems through suitable diagnostic biomarkers. The study focuses on two clinical issues, reversible versus irreversible pulpitis and persistent idiopathic dentoalveolar pain, to demonstrate the role of biomarkers in understanding complex dental pathologies and guiding appropriate treatment strategies.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Frank Sanner, David Sonntag, Norbert Hambrock, Matthias Zehnder
Summary: Persistent idiopathic dentoalveolar pain (PIDAP) mainly affects females and is typically associated with undisturbed sleep and periodontal allodynia.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Viviane A. Klemmer, Nupur Khera, Barbara M. Siegenthaler, Indranil Bhattacharya, Franz E. Weber, Chafik Ghayor
Summary: The human skeleton is a dynamic and highly organized organ system. N-Vinyl-2-pyrrolidone (NVP) enhances the effects of BMP2 on osteoblast and osteoclast differentiation, promoting bone regeneration. NVP may prove useful in situations requiring high rates of bone regeneration and in managing bone diseases associated with excessive bone resorption like osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
N. Ballal, H. F. Duncan, D. B. Wiedemeier, N. Rai, P. Jalan, V Bhat, V. S. Belle, M. Zehnder
Summary: In this study, it was found that the levels of neutrophil gelatinase 9 (MMP-9)/total protein (TP) and NaOCl lavage significantly influenced pulp survival in cariously exposed and directly capped teeth, while mild preoperative discomfort did not have an impact.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Chemistry, Physical
Chafik Ghayor, Indranil Bhattacharya, Julien Guerrero, Mutlu oezcan, Franz E. Weber
Summary: This study investigates the role of microarchitecture and nanoarchitecture of scaffolds in osteoconduction. The results show that microporosity of the scaffolds does not affect bony ingrowth or osteoclastic resorbability. This research is important for preventing non-unions during bone regeneration procedures.
Article
Chemistry, Multidisciplinary
Prakan Thanasrisuebwong, Julian R. Jones, Salita Eiamboonsert, Nisarat Ruangsawasdi, Bundhit Jirajariyavej, Parichart Naruphontjirakul
Summary: Zn-containing dense monodispersed bioactive glass nanoparticles have been developed to deliver therapeutic inorganic trace elements to cells for stimulating bone regeneration. These nanoparticles show no cytotoxicity and can stimulate osteogenic differentiation in PDLSCs.
Article
Dentistry, Oral Surgery & Medicine
Anas Al-Jadaa, Zehraa Saidi, Maise Mahmoud, Rahaf Al-Taweel, Matthias Zehnder
Summary: This study compared the in vitro efficacy and safety of different irrigant activation devices in apically closed and open root canal systems. The results showed that ultrasonic agitation was more effective in cleaning the central area of the root canal, while it had a lower effect in cleaning the simulated periapical lesion. Therefore, ultrasonic agitation was found to be more targeted and safer after the administration of sodium hypochlorite.
JOURNAL OF ENDODONTICS
(2023)
Article
Biotechnology & Applied Microbiology
Christophe Drouet, Nicolas Vandecandelaere, Anke Burger-Kentischer, Iris Trick, Christina G. Kohl, Tanja Maucher, Michaela Mueller, Franz E. Weber
Summary: Biomimetic nanocrystalline apatites analogous to bone mineral can be prepared using soft chemistry. However, immersion in aqueous media of dried non-carbonated biomimetic apatite crystals may generate an acidification event, which could limit their further development. In this study, the acidification phenomenon was explored, and a practical strategy to circumvent it was proposed. The non-cytotoxicity and antibacterial features of the post-equilibrated nanocrystalline apatites were demonstrated, suggesting their relevance in the field of antibacterial bone substitutes.
BIOENGINEERING-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Julia Rieber, Gabriella Meier-Burgisser, Iris Miescher, Franz E. Weber, Petra Wolint, Yang Yao, Esteban Ongini, Athanasios Milionis, Jess G. Snedeker, Maurizio Calcagni, Johanna Buschmann
Summary: Tendon injuries can result in limited range of motion and poor biomechanical outcomes. A novel three-layer tube based on polymer DegraPol with incorporated IGF-1 was developed using emulsion electrospinning. The IGF-1-containing tubes exhibited sustained release of the growth factor and showed bioactivity by upregulating gene expression. They also demonstrated superior mechanical properties compared to pure DP tubes. These tubes may help accelerate the healing process and reduce adhesion formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Julien Guerrero, Ekaterina Maevskaia, Chafik Ghayor, Indranil Bhattacharya, Franz E. Weber
Summary: This study utilized additive manufacturing to produce two different diameter tricalcium phosphate-based bone scaffolds and observed them in vivo for 10 days followed by gene sequencing and histological analysis. The results showed upregulation of genes related to immune response, cell adhesion, and cell migration in both scaffolds. However, only the scaffold with a 0.50mm diameter showed significant overexpression of genes involved in angiogenesis, cell differentiation regulation, ossification, and bone development. Immunohistochemistry and high-resolution CT scans also revealed more blood vessels and mineralized tissue in the 0.50mm scaffold. Therefore, different filament diameters significantly influence angiogenesis and cell differentiation regulation in the early phase of bone regeneration, impacting the overall clinical outcome.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Vishal Sehgal, Nisarat Ruangsawasdi, Sirichai Kiattavorncharoen, Sompop Bencharit, Prakan Thanasrisuebwong
Summary: This study examined the effects of different collagen membranes on barrier function and tissue integration. The results showed that all collagen membranes allowed some cell migration, and crosslinking did not improve barrier ability. Native collagen membranes provided a better surface for gingival cell attachment and proliferation.
Review
Materials Science, Composites
Fereshte Rezaie, Masoud Farshbaf, Mohammad Dahri, Moein Masjedi, Reza Maleki, Fatemeh Amini, Jonathan Wirth, Keyvan Moharamzadeh, Franz E. Weber, Lobat Tayebi
Summary: Revolutionary fabrication technologies like 3D printing are expected to replace traditional methods in dental structure development due to their ability to create constructs with required properties and detailed structures. This review article explores the recent applications of 3D printing techniques in dental prosthetics manufacturing, including various printing techniques, materials used, and limitations/challenges faced. The article also utilizes major scientific databases to find relevant literature on dental prostheses 3D printing.
JOURNAL OF COMPOSITES SCIENCE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Kevin Hofpeter, Matthias Zehnder, Michael Huelsmann, Anas Al-Jadaa, Shengjile Deari
Summary: This study aimed to develop a standardized curved root canal model in bovine dentine and compare its behavior to resin in standard plastic training blocks during chemo-mechanical preparation. The study found that dentine had higher microhardness compared to resin, and dentine exhibited greater canal transportation, which was slightly increased by EDTA. The results are important for testing and comparing materials and concepts of chemo-mechanical root canal instrumentation.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)