Article
Biochemistry & Molecular Biology
Chaoren Yan, Xu Shao, Qi Shu, Yonggang Teng, Youbei Qiao, Ping Guan, Xiaoling Hu, Chaoli Wang
Summary: The designed ultra-thin hollow silica nanoparticles (UHSN) and covalent anchoring of chitosan (UHSN@CS) can enhance the loading and photodynamic property of photosensitizers, thus improving antibacterial performance.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Ziming Yang, Puwang Li, Yu Chen, Qiang Gan, Zhipan Feng, Yiguang Jin, Chuang Zhou, Zuyu He, Chao Wang, Yunhao Liu, Changgen Feng
Summary: A novel pH/glutathione multi-responsive chitosan nanoparticles material has been successfully designed and prepared for photodynamic therapy, providing a new strategy for targeted photosensitizer delivery. The nanoparticles have high ICG entrapment and loading efficiency, achieving multi-responsive ICG release in tumor cells with strong therapeutic effects.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Chemistry, Multidisciplinary
Jipsa Chelora, Yuchao Liang, Wei-Chih Wei, Xiao Cui, Yafang Xiao, Yingpeng Wan, Zhongming Huang, Shuang Tian, Shengliang Li, Yi-Hsuan Huang, Ken-Tsung Wong, Chun-Sing Lee
Summary: The development of small molecule-based nanoparticles that can perform effective photothermal therapy and overcome hypoxia for cancer photodynamic therapy shows promising results in vitro and in vivo studies.
Article
Chemistry, Analytical
Shengqiang Hu, Lixian Huang, Liuyan Zhou, Tingchan Wu, Shulin Zhao, Liangliang Zhang
Summary: This research focuses on the integration of ratiometric NIR-II fluorescence imaging and chemo-/photodynamic combination therapy for accurate diagnosis and efficient treatment of deep tumors. By conjugating dual-ligands-stabilized gold nanoclusters (cgAuNCs) into down-/up-conversion nanoparticles (D/UCNPs), single-excitation triple-emission nanoassemblies were prepared, enabling simultaneous imaging and therapy upon exposure to an 808 nm laser. The study provides insights into the design of multifunctional nanoplatforms for tumor diagnosis and treatment.
ANALYTICAL CHEMISTRY
(2023)
Review
Immunology
Cecilia de Brito Barbosa, Isabela Monici Silva, Jessica Alves de Cena, Cristine Miron Stefani, Naile Dame-Teixeira
Summary: This study aims to conduct a systematic review to comprehensively assess the profile of host and bacterial-derived collagenolytic proteases in both root and coronal dentin carious lesions.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Chemistry, Inorganic & Nuclear
Zhiheng Li, Zhenwu Zhou, Yarui Wang, Jie Wang, Liming Zhou, Hong-Bo Cheng, Juyoung Yoon
Summary: Due to deficiencies in specific accumulation at tumors and undesired activation in normal tissues, many photodynamic therapy (PDT) agents show off-target phototoxicity. To address this issue, activatable photodynamic nano-photosensitizers (nanoPSs) that generate reactive oxygen species (ROS) in response to tumor-associated or external stimuli have been developed. These nanoPSs are in an off-state during delivery and are only activated in targeted lesions, enhancing the accuracy and minimizing side effects. This review discusses the design, work principle, and therapeutic performance of PDT using activatable nanoPSs, categorized by different stimuli and composite materials.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Mariana Miretti, Cesar G. Prucca, Maria T. Baumgartner, Marisa Martinelli
Summary: Photodynamic therapy is a promising alternative treatment for various pathologies, including cancer. However, the low solubility of photosensitizers is a limitation that needs to be addressed. In this study, a film composed of chitosan, polyvinylpyrrolidone, and liposomes containing Zn-phthalocyanine was synthesized and characterized. The film showed excellent photophysical properties and demonstrated high efficacy in inhibiting cell viability in vitro.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Bing Lu, Zhecheng Zhang, Danni Jin, Xiaolei Yuan, Jin Wang, Yue Ding, Yang Wang, Yong Yao
Summary: New nanoparticles (Y6 NPs) based on the A-DA ' D-A fused-ring conjugated small molecule Y6 have been developed for the photothermal and photodynamic treatment of cancer. These Y6 NPs demonstrate outstanding light absorption, good photothermal conversion efficiency, and reactive oxygen species generation capability. The high photothermal conversion efficiency and excellent photodynamic activity make them promising for cancer therapy.
CHEMICAL COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Amanda Leticia Polli Silvestre, Aline Martins dos Santos, Analu Barros de Oliveira, Tulio Morandin Ferrisse, Fernanda Lourencao Brighenti, Andreia Bagliotti Meneguin, Marlus Chorilli
Summary: This study aimed to develop and evaluate nanoparticles based on chitosan and sodium alginate encapsulated with CUR, as well as polymeric films associated with aPDT in oral biofilms. The results showed that the nanoparticles controlled the release of CUR for a longer period, which makes them promising carriers for oral CUR delivery.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemical Research Methods
Mingdang Li, Wei Feng, Menglong Zhao, Peiling Dai, Yucheng Zhou, Jiawei Wang, Wen Lv, Shujuan Liu, Qiang Zhao
Summary: The study developed a polymeric therapeutic agent to overcome tumor hypoxia through multiple pathways, achieving capture and controllable release of O-1(2). It displayed promising synergistic anti-tumor and anti-recurrence efficacies under hypoxic environments, suggesting new avenues for the design of treatment platforms in clinical studies.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Shasha Zhao, Kexin Lai, Zhen Gao, Xueli Ye, Juan Mou, Shiping Yang, Huixia Wu
Summary: This article introduces a clever combination of nano-enzymes and therapeutic drugs that can promote the production of reactive oxygen species (ROS) in cancer cells, enhancing the therapeutic efficacy of nanomedicines on malignant tumors. By loading the specific drug onto PEGylated Ce-doped hollow mesoporous silica nanoparticles, a smart nanoplatform is constructed to improve tumor therapy efficiency. The carrier showed multi-enzyme activities, with Ce3+ ions converting endogenous H2O2 into highly toxic OH for chemodynamic therapy and Ce4+ ions exhibiting catalase-like activity to reduce tumor hypoxia and glutathione (GSH) peroxidase-mimicking properties to effectively deplete GSH in tumor cells. Moreover, the loaded drug disrupts mitochondrial functions, causing the accumulation of superoxide anions (O-2(-)) and H2O2 within tumor cells. By combining the advantages of the carrier and the drug, the prepared nanoplatform can efficiently induce cancer cell death and inhibit tumor growth through significantly enhanced ROS production. Therefore, this positive combination therapy strategy has a promising application prospect for enhancing antitumor efficacy.
Article
Dentistry, Oral Surgery & Medicine
Nandini Suresh, Haritha Jamuna Subbarao, Velmurugan Natanasabapathy, Anil Kishen
Summary: Photoactivated chitosan-based nanoparticles have been shown to effectively eliminate bacterial biofilm, inactivate endotoxins, and promote bone regeneration. This study presented successful healing outcomes in cases of extensive inflammatory root resorption using a combination of surgical and nonsurgical root canal therapy with rose bengal functionalized chitosan nanoparticles. Postoperative follow-up demonstrated significant osseous healing and type I pattern of root fracture healing.
JOURNAL OF ENDODONTICS
(2021)
Article
Chemistry, Multidisciplinary
F. A. Sewid, K. Annas, A. Dubavik, A. V. Veniaminov, V. G. Maslov, A. O. Orlova
Summary: Water-soluble nanocomposites based on CdSe/ZnS quantum dots and hydrophobic TPP molecules encapsulated by CS have been formed, with MCD spectra showing the presence of TPP in both monomeric and agglomerated forms. These nanocomposites demonstrate enhanced singlet oxygen generation compared to free TPP in CS at the same concentration, with an average efficiency of 45% due to intracomplex FRET.
Review
Biochemistry & Molecular Biology
Xudong Li, Yuran Wang, Chenyun Feng, Haijun Chen, Yu Gao
Summary: This review discusses the application of chitosan in cancer theranostics, including its chemical functionalities, synthesis methods, and the improvement of treatment efficiency through functionalization. Several case studies of chitosan-based nanotheranostics are also reported.
Article
Chemistry, Multidisciplinary
Wenbo Deng, Meng Jia, Quan Shi, Yunhao Xu, Yaqing Feng, Yanming Zhao, Min Gong, Bao Zhang
Summary: Nanoscale metal-organic polymers (MOPs) based on corrole have shown great potential in photodynamic therapy (PDT) and photothermal therapy (PTT). In this study, a unique corrole-based MOP (PFCC-Hf) was successfully synthesized using a simple solvothermal method. The PFCC-Hf demonstrated superior synergistic PDT and PTT efficacies compared to traditional PDT treatment, with a high apoptosis rate of A375 cells in vitro. This work highlights the potential of corrole-based MOPs for efficient phototherapy treatments.
MATERIALS CHEMISTRY FRONTIERS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Gillian Landzberg, Hebatullah Hussein, Anil Kishen
Summary: A novel 2-part varnish system containing chitosan nanoparticles and chitosan-grafted hydroxyapatite precursor nanocomplex was developed for conditioning and sealing root-end dentin, displaying significant antibacterial activity and promoting fibroblast attachment. The system effectively reduced bacterial load and formed a biomineralized layer, offering optimal conditions for healing and improving prognosis in teeth with root-end cracks after endodontic microsurgery.
JOURNAL OF ENDODONTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Tyler Phaneuf, Anil Kishen, Massieh Moayedi, Ernest W. N. Lam
Summary: Diagnosing VRF remains a challenge for clinicians, where CBCT imaging plays an important role but faces limitations such as beam hardening and streak artifacts.
JOURNAL OF ENDODONTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Muna Marashdeh, Cameron Stewart, Anil Kishen, Celine Levesque, Yoav Finer
Summary: This study evaluated the antimicrobial activity, drug release, and flow of root canal sealers modified with novel highly loaded antimicrobial drug-silica coassembled particles. The findings suggest that the modified sealers may offer potential antimicrobial benefits in root canal treatment, although they also had an impact on the flow properties of the sealers.
JOURNAL OF ENDODONTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Nandini Suresh, Haritha Jamuna Subbarao, Velmurugan Natanasabapathy, Anil Kishen
Summary: Photoactivated chitosan-based nanoparticles have been shown to effectively eliminate bacterial biofilm, inactivate endotoxins, and promote bone regeneration. This study presented successful healing outcomes in cases of extensive inflammatory root resorption using a combination of surgical and nonsurgical root canal therapy with rose bengal functionalized chitosan nanoparticles. Postoperative follow-up demonstrated significant osseous healing and type I pattern of root fracture healing.
JOURNAL OF ENDODONTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Hebatullah Hussein, Anil Kishen
Summary: The study demonstrated that CSnp/CMCS medication facilitated the switch of MQ towards M2 (regulatory/anti-inflammatory) phenotype and promoted PdLF migration through paracrine signaling.
JOURNAL OF ENDODONTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Muna Q. Marashdeh, Cameron A. Stewart, Anil Kishen, Celine Levesque, Yoav Finer
Summary: The study demonstrates that the addition of novel antimicrobial drug-silica coassembled particles (DSPs) to root canal sealers can effectively reduce Enterococcus faecalis infection in root canal dentin. This modified sealer exhibited significant antimicrobial activity and decreased the incidence of secondary infections, highlighting the potential of DSP-loaded sealers in endodontic treatment.
JOURNAL OF ENDODONTICS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Preethi Poornima, Jogikalmat Krithikadatta, Ratna Rachel Ponraj, Natanasabapathy Velmurugan, Anil Kishen
Summary: This parallel arm trial compared the efficacy of chitosan-based varnish and chlorhexidine-fluoride varnish in reducing bacterial count in patients undergoing fixed orthodontic therapy. Both varnishes showed similar anti-plaque effects, with no impact on plaque pH over a six-week period.
Article
Dentistry, Oral Surgery & Medicine
Hengameh Bakhtiar, Azin Ashoori, Sarah Rajabi, Mohamad Pezeshki-Modaress, Alireza Ayati, Mohammad Reza Mousavi, Mohammad Reza Ellini, Amir Kamali, Amir Azarpazhooh, Anil Kishen
Summary: The 30 mg/ml HAM ECM scaffold exhibited optimal physical properties and enhanced hDPSC migration. The HAM ECM scaffold did not hinder the formation of pulp-like tissue and revascularization within the root canal when used as both cell-free and cell-loaded scaffold. These findings underscore the potential of HAM ECM membrane for further exploration in regenerative endodontics.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Kaviya Baskar, Balasubramanian Saravana Karthikeyan, Ishwarya Gurucharan, Sekar Mahalaxmi, Gurusamy Rajkumar, Vijayakumar Dhivya, Anil Kishen
Summary: This study aimed to evaluate the odontogenic differentiation abilities of porous biomineralizable composite scaffolds made of HAnp and CMC on hDPSCs, with the 1:5 HAnp-CMC scaffold demonstrating favorable cell seeding properties and increased cell viability/proliferation, along with enhanced expressions of DSPP and VEGF.
INTERNATIONAL ENDODONTIC JOURNAL
(2022)
Article
Dentistry, Oral Surgery & Medicine
Kamna Singh, Aiman Ali, Annie Shrestha, Marco Magalhaes, Anil Kishen
Summary: Disinfection of LPS-contaminated dentin with CSRBnps demonstrated M2-type polarization of macrophages, which corresponded to repair and neotissue formation.
JOURNAL OF ENDODONTICS
(2022)
Review
Dentistry, Oral Surgery & Medicine
Amir Azarpazhooh, Saber Khazaei, Hamid Jafarzadeh, Gevik Malkhassian, Adam Sgro, Mohamed Elbarbary, Elaine Cardoso, Ariel Oren, Anil Kishen, Prakesh S. Shah
Summary: This study proposes a standardized data collection template and checklist for reporting various study designs on endodontic outcome, aiming to promote standardization among clinicians and researchers to support evidence-based treatment decisions.
JOURNAL OF ENDODONTICS
(2022)
Review
Dentistry, Oral Surgery & Medicine
Amir Azarpazhooh, Elaine Cardoso, Adam Sgro, Mohamed Elbarbary, Nima Laghapour Lighvan, Rana Badewy, Gevik Malkhassian, Hamid Jafarzadeh, Hengameh Bakhtiar, Saber Khazaei, Ariel Oren, Madeline Gerbig, Helen He, Anil Kishen, Prakesh S. Shah
Summary: Despite efforts to standardize reporting, variations in study design and outcomes assessed persist in endodontic research. This scoping review identified an increase in the quantity of endodontic outcome publications and an improvement in the levels of evidence over the past four decades. Studies focusing on nonsurgical retreatment and root canal treatment, especially in older patients and molars, have increased in recent years, but challenges in data standardization and reporting inconsistencies remain.
JOURNAL OF ENDODONTICS
(2022)
Review
Dentistry, Oral Surgery & Medicine
Amir Azarpazhooh, Adam Sgro, Elaine Cardoso, Mohamed Elbarbary, Nima Laghapour Lighvan, Rana Badewy, Gevik Malkhassian, Hamid Jafarzadeh, Hengameh Bakhtiar, Saber Khazaei, Ariel Oren, Madeline Gerbig, Helen He, Anil Kishen, Prakesh S. Shah
Summary: The main outcomes in endodontic studies include success, radiographic healing, survival, pain assessment, and quality of life, but patient-centered outcome measures are lacking. There is a need to further develop patient-centered outcome measures in endodontics.
JOURNAL OF ENDODONTICS
(2022)
Article
Engineering, Biomedical
Hebatullah Hussein, Anil Kishen
Summary: This study found that engineered bioactive chitosan-based nanoparticles can modulate the protein and cytokine profiles of macrophages, reducing inflammation caused by apical periodontitis and promoting the migration of periodontal ligament fibroblasts.
BIOACTIVE MATERIALS
(2022)
Review
Pharmacology & Pharmacy
Farzaneh Afkhami, Parisa Forghan, James L. Gutmann, Anil Kishen
Summary: The limitations of conventional root canal disinfection strategies and antimicrobials have affected the efficient elimination of microorganisms and their byproducts from infected root canals. Silver nanoparticles (AgNPs) have wide-spectrum anti-microbial activity, making them advantageous for root canal disinfection. Compared to other nanoparticulate antibacterials, AgNPs have acceptable antibacterial properties and relatively low cytotoxicity. They penetrate deeper into the complexities of the root canal systems and dentinal tubules, enhancing the antibacterial properties of endodontic irrigants and sealers.