Article
Cell & Tissue Engineering
L. L. E. Whitehouse, N. H. Thomson, T. Do, G. A. Feichtinger
Summary: Research in dental regeneration is focusing on dental-pulp capping, with natural biomimetic scaffolds showing potential advantages over manufactured materials. Further exploration of synergistic peptide use and tailored scaffold development is needed for the successful application in dental-pulp capping.
EUROPEAN CELLS & MATERIALS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Duo Xu, Noriko Mutoh, Hayato Ohshima, Nobuyuki Tani-Ishii
Summary: This study investigated the effect of MTA on pulp healing in infected and uninfected pulp tissue during direct pulp capping. The results suggest that MTA promotes pulpal healing, while CH does not facilitate the differentiation of odontoblast-like cells.
DENTAL MATERIALS JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Anna Zawadzka-Knefel, Agnieszka Rusak, Monika Mrozowska, Tomasz Machalowski, Andrzej Zak, Katarzyna Haczkiewicz-Lesniak, Michal Kulus, Piotr Kuropka, Marzenna Podhorska-Okolow, Katarzyna Skoskiewicz-Malinowska
Summary: The use of stem cells for tissue regeneration is a prominent trend in regenerative medicine and tissue engineering. Dental pulp stem cells (DPSCs) have attracted attention due to their ability to differentiate into osteoblasts and odontoblasts. This study explored the biological responses of human DPSCs to a purified 3D chitin scaffold derived from the marine demosponge Aplysina fistularis. Results showed that the chitin scaffold, especially with hydroxyapatite (HAp) modification, supported cell adhesion, proliferation, and differentiation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Cell Biology
Kajohnkiart Janebodin, Rakchanok Chavanachat, Aislinn Hays, Morayma Reyes Gil
Summary: The study aimed to investigate the role of VEGFR-2 in odontoblast differentiation of dental pulp stem cells (DPSCs) by knocking down the expression of VEGFR-2. The results showed that VEGFR-2 is crucial for odontoblast differentiation in DPSCs, and deficiency of VEGFR-2 may impair the differentiation of odontoblast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, General & Internal
Pratima Panda, Shashirekha Govind, Sanjit Kumar Sahoo, Satabdi Pattanaik, Rachappa M. Mallikarjuna, Triveni Nalawade, Sanjay Saraf, Naseer Al Khaldi, Salma Al Jahdhami, Vinay Shivagange, Amit Jena
Summary: The study examined the effects of different pulp capping agents on human dental pulp stem cells. The results showed that platelet-rich fibrin (PRF) had the highest cell viability and the greatest potential to enhance cell proliferation and differentiation. On the other hand, Tetric N-Bond was found to be ineffective as a pulp capping agent. Overall, PRF was the most effective in maintaining the viability of human dental pulp stem cells compared to the other tested materials.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Dentistry, Oral Surgery & Medicine
Xiangfen Li, Liu Wang, Qin Su, Ling Ye, Xuedong Zhou, Lan Zhang, Dongzhe Song, Dingming Huang
Summary: The study demonstrated that BMP9 plays an important role in promoting mineralization of dental pulp cells and dentinogenesis, making it a potential therapeutic target for repairing dental pulp injury.
JOURNAL OF ENDODONTICS
(2021)
Article
Engineering, Biomedical
Matthew J. Osmond, Melissa D. Krebs
Summary: The study introduces a potential regenerative pulp capping agent by embedding calcium phosphate nanoparticles in carboxymethyl-chitosan and a diglycidyl ether polymer hydrogel, demonstrating promising potential for restoring tooth function and healthy dentin tissue.
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
(2021)
Article
Cell Biology
Keita Ipposhi, Atsushi Tomokiyo, Taiga Ono, Kozue Yamashita, Muhammad Anas Alhasan, Daigaku Hasegawa, Sayuri Hamano, Shinichiro Yoshida, Hideki Sugii, Tomohiro Itoyama, Marina Ogawa, Hidefumi Maeda
Summary: SFRP1 plays a crucial role in dentinogenesis and promoting reparative dentin formation, as demonstrated by its effects on odontoblastic differentiation and mineralized nodule formation. The strong expression of SFRP1 in mature odontoblasts indicates its importance in dental pulp preservation and response to injury. Additionally, SFRP1 regulates odontoblast-related gene expression, particularly BMP-2, affecting the formation and structure of reparative dentin.
Article
Biochemistry & Molecular Biology
Maki Kimura, Hiroyuki Mochizuki, Ryouichi Satou, Miyu Iwasaki, Eitoyo Kokubu, Kyosuke Kono, Sachie Nomura, Takeshi Sakurai, Hidetaka Kuroda, Yoshiyuki Shibukawa
Summary: The study revealed the crucial role of PMCA in maintaining calcium homeostasis and promoting mineralization in odontoblasts, especially in the Ca2+ extrusion pathway under hypotonic and alkaline stimulation. Additionally, PMCA is involved in dentinogenesis induced by mechanical or direct alkaline stimulation in both physiological and pathological conditions.
Article
Dentistry, Oral Surgery & Medicine
Chawan Manaspon, Chavin Jongwannasiri, Sujin Chumprasert, Noppadol Sa-Ard-Iam, Rangsini Mahanonda, Prasit Pavasant, Thantrira Porntaveetus, Thanaphum Osathanon
Summary: The study found that ProRoot(R) MTA and Biodentine(TM) have favorable in vitro biocompatibility and bioactive properties on human dental pulp stem cells, suggesting their superior regenerative potential compared with DyCal(R) and TheraCal(TM).
Article
Materials Science, Ceramics
Ensanya A. Abou Neel, Soumya Sheela, Fatma Mousa AlGhalban, Andi Arjuna, Ifty Ahmed
Summary: This study investigated the morphology, chemistry, thermal characteristics, cytocompatibility, and osteogenic potential of solid and porous phosphate-based glass microspheres for potential use as pulp-capping materials. Through various tests, it was found that both solid and porous GMs showed good compatibility with dental pulp stem cells and could support cell attachment, spreading, and viability. However, cells grown in GMs' extracts showed higher viability but less mineralization compared to direct contact with GMs. Therefore, both types of GMs have potential applicability as pulp-capping materials.
JOURNAL OF NON-CRYSTALLINE SOLIDS
(2023)
Article
Cell Biology
Milos Papic, Suzana Zivanovic, Tamara Vucicevic, Miona Vuletic, Mirjana V. Papic, Nevena Milivojevic, Ana Miric, Marina Miletic Kovacevic, Marko Zivanovic, Milan Stamenkovic, Vladimir Zivkovic, Slobodanka Mitrovic, Vladimir Jakovljevic, Biljana Ljujic, Milica Popovic
Summary: The study aimed to evaluate the dental pulp responses to rhEPO and/or MTA in pulp capping of inflamed dental pulp in vivo. Pulp capping with rhEPO or MTA + rhEPO resulted in lower inflammation and higher mineralization scores compared to untreated control. Capping with rhEPO or MTA + rhEPO resulted in a favorable effect that was similar or even superior to that of MTA.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2023)
Article
Physiology
Huihui Ren, Quan Wen, Qingxuan Zhao, Nan Wang, Yuming Zhao
Summary: Dental pulp plays a crucial role in maintaining tooth function and pulp-regeneration therapy shows promise in clinical practice. However, the mechanisms of pulp regeneration and the role of dental stem cells are not fully understood. This study used single-cell RNA sequencing analysis to explore the development of dental pulp and stem cells at different stages. The results revealed temporal changes in the composition of pulp cells and identified subpopulations of odontoblasts and mesenchymal stem cells. These findings may provide insights into tooth development and improve the success of dental pulp regeneration.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Cell Biology
Daisuke Omagari, Taku Toriumi, Hiromasa Tsuda, Manabu Hayatsu, Keisuke Watanabe, Yusuke Mizutami, Masaki Honda, Yoshikazu Mikami
Summary: This study investigated the role of sortilin 1 (SORT1) in the odontoblastic differentiation of human dental pulp-derived stem cells (hDPSCs). SORT1 expression was found in hDPSCs expressing the mesenchymal stem cell markers CD44 and CD90. SORT1-positive hDPSCs showed higher alkaline phosphatase (ALP) staining, indicating enhanced odontoblastic differentiation. The interactions between SORT1 and p75NTR, as well as the involvement of the JUN N-terminal kinases (JNK)/JUN signaling pathway, were also demonstrated.
Article
Cell & Tissue Engineering
Xinghong Luo, Weiqing Feng, Shijiang Huang, Shenghong Miao, Tao Jiang, Qian Lei, Jingyao Yin, Sheng Zhang, Xiaochun Bai, Chunbo Hao, Weizhong Li, Dandan Ma
Summary: mTORC1 activation inhibits odontoblastic differentiation of dental pulp stem cells by suppressing exosome release from odontoblasts. These findings provide new insights into dental pulp complex regeneration.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Andrea Gama, Jorge William Vargas-Franco, Diana Carolina Sanchez Mesa, Elizabeth Restrepo Bedoya, Jerome Amiaud, Sylvie Babajko, Ariane Berdal, Ana Carolina Acevedo, Dominique Heymann, Frederic Lezot, Beatriz Castaneda
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Medicine, General & Internal
Andrea Gama, Laura Maman, Jorge William Vargas-Franco, Rana Omar, Benedicte Brounais-Le Royer, Hideo Yagita, Sylvie Babajko, Ariane Berdal, Ana Carolina Acevedo, Dominique Heymann, Frederic Lezot, Beatriz Castaneda
JOURNAL OF CLINICAL MEDICINE
(2020)
Article
Orthopedics
Anne Morice, Aline Joly, Manon Ricquebourg, Gerard Maruani, Emmanuel Durand, Louise Galmiche, Jeanne Amiel, Yoann Vial, Helene Cave, Kahina Belhous, Marie Piketty, Martine Cohen-Solal, Ariane Berdal, Corinne Collet, Arnaud Picard, Amelie E. Coudert, Natacha Kadlub
BMC MUSCULOSKELETAL DISORDERS
(2020)
Article
Dentistry, Oral Surgery & Medicine
R. Arruda-Vasconcelos, L. M. Louzada, M. Feres, P. L. Tomson, P. R. Cooper, B. P. F. A. Gomes
Summary: The microbial profile, and levels of endotoxin (LPS) and lipoteichoic acid (LTA), in infected dentine and root canals of teeth with irreversible pulpitis were investigated. Root canal preparation effectively reduced the levels of bacteria, LPS and LTA, while intracanal medication did not significantly reduce bacterial levels but led to a significant decrease in LTA levels.
INTERNATIONAL ENDODONTIC JOURNAL
(2021)
Article
Dentistry, Oral Surgery & Medicine
Kubra Aral, Michael R. Milward, Paul R. Cooper
Summary: This study aimed to investigate the potential regulatory pathways of IL-1 beta production by monitoring the effects of periodontal pathogens Fn and Pg on inflammasomes and their regulators in HGFs. The results showed that Fn and Pg, especially when combined with ATP exposure, could dysregulate NLRP3, AIM2, IL-1 beta, ASC, and other inflammasome components and regulators, ultimately affecting the production of IL-1 beta.
Article
Chemistry, Physical
David Yong, Joanne Jung Eun Choi, Peter Cathro, Paul R. Cooper, George Dias, Jeffrey Huang, Jithendra Ratnayake
Summary: The aim of this study was to develop an endodontic cement using bovine bone-derived hydroxyapatite (BHA), Portland cement (PC), and a radiopacifier. The results showed that the addition of 10% and 20% BHA improved the mechanical strength of the cement while maintaining similar radiopacity and biocompatibility to ProRoot MTA. This study is important for enhancing the properties of endodontic cements.
Article
Endocrinology & Metabolism
Victor Simancas Escorcia, Clement Guillou, Lilia Abbad, Louise Derrien, Claudio Rodrigues Rezende Costa, Vidjea Cannaya, Mourad Benassarou, Christos Chatziantoniou, Ariane Berdal, Ana Carolina Acevedo, Olivier Cases, Pascal Cosette, Renata Kozyraki
Summary: Enamel renal syndrome (ERS) is a rare disorder caused by mutations in FAM20A, which may lead to gingival fibromatosis. Proteomic analysis revealed dysregulation of proteins related to extracellular matrix organization and mineralization in ERS patients, suggesting that TGF beta pathway could play a central role in the pathogenesis.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Environmental Sciences
Ai Thu Bui, Sophia Houari, Sophia Loiodice, Dominique Bazin, Jeremy Sadoine, Nicolas Roubier, Elsa Vennat, Thu Thuy Tran, Ariane Berdal, Jean-Marc Ricort, Sakina Mhaouty-Kodja, Sylvie Babajko
Summary: This study analyzed the effects of exposure to low doses of DEHP on mouse teeth. The results showed that DEHP can directly affect dental cells, leading to impaired enamel development. Additionally, the study found delayed effects of DEHP on enamel hardness and mineralization. These findings are important for understanding the impact of environmental toxicant exposure and providing early markers.
ENVIRONMENTAL HEALTH PERSPECTIVES
(2022)
Article
Biochemistry & Molecular Biology
Shelly Arora, Paul R. Cooper, Lara T. Friedlander, Benedict Seo, Shakila B. Rizwan, Alison M. Rich, Haizal Mohd Hussaini
Summary: This study successfully isolated and cultured cHDPCs and ncHDPCs from healthy and inflamed dental pulp tissue respectively. The growth characteristics and differentiation abilities were not significantly different between the two types of HDPCs. However, the expression of certain genes such as TLR-2 and IL-6 was significantly higher in cHDPCs, indicating an inflammatory phenotype in the inflamed dental pulp.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zohaib Khurshid, Mohammed Farhan Alfarhan, Javed Mazher, Yasmin Bayan, Paul R. Cooper, George J. Dias, Necdet Adanir, Jithendra Ratnayake
Summary: In this study, camel bone-based hydroxyapatite (CBHA) was extracted and characterized. The CBHA exhibited favorable mechanical properties, porous structure, and stability in simulated body fluid, making it a promising material for bone tissue regeneration applications.
Article
Biochemistry & Molecular Biology
Michaela Kearney, Paul R. Cooper, Anthony J. Smith, Henry F. Duncan
Summary: Exciting opportunities exist within regenerative endodontics for the development of targeted biomaterials that harness epigenetic machinery to control pulpitis and stimulate repair. In this study, small RNA sequencing and bioinformatic analysis were used to establish a miRNA expression profile for mineralising dental pulp cells (DPCs) and examine their interaction with histone deacetylase inhibitors and DNA methyltransferase inhibitors. The results showed that these inhibitors induced mineralisation but reduced cell growth, suggesting an increased and dynamic interaction between miRNA and epigenetic modifiers during DPC reparative processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Correction
Dentistry, Oral Surgery & Medicine
Rabia Majeed, Hoda Mohamed Elnawawy, Muralithran Govindan Kutty, Noor Azlin Yahya, Noor Hayati Azami, Noor Hayaty Abu Kasim, Mohamed Shady Nabhan, Paul Roy Cooper, Josette Camilleri, Hany Mohamed Aly Ahmed
Review
Dentistry, Oral Surgery & Medicine
Rabia Majeed, Hoda Mohamed Elnawawy, Muralithran Govindan Kutty, Noor Azlin Yahya, Noor Hayati Azami, Noor Hayaty Abu Kasim, Mohamed Shady Nabhan, Paul Roy Cooper, Josette Camilleri, Hany Mohamed Aly Ahmed
Summary: This systematic review examined the effects of nano-sized cement particles on the properties of calcium silicate-based cements. A literature search was conducted and 17 eligible studies were included. The results showed that nano-calcium silicate-based cements have better physical, mechanical, and biological properties compared to commonly used cements. However, there were deficiencies in characterizing and verifying the nano-particle size, and the presence of additives may have influenced the properties.
Review
Dentistry, Oral Surgery & Medicine
Eisha Imran, Paul R. Cooper, Jithendra Ratnayake, Manikandan Ekambaram, May Lei Mei
Summary: This article provides a contemporary review of the potential beneficial effects of nanosized hydroxyapatite (nHAP) on caries lesions demonstrated in in vitro studies. Data showed that nHAP has the potential to promote mineralization in initial caries and has antimicrobial effects through metal substitution. Metal-substituted fluorhydroxyapatite nanoparticles can potentially possess dual action property and enhanced chemical stability and bioactivity, providing a promising synergistic strategy for the development of dental therapeutics.
Article
Dentistry, Oral Surgery & Medicine
Satnam S. Virdee, Nasir Z. Bashir, Milan Krstic, Josette Camilleri, Melissa M. Grant, Paul R. Cooper, Phillip L. Tomson
Summary: This study identified optimized parameters for periapical tissue fluid (PTF) paper point sampling and compared the proteomes of PTF from teeth with normal apical tissues (NAT) and asymptomatic apical periodontitis (AAP) using high-throughput proteomic analysis. The results showed that some proteins were closely associated with AAP and had good diagnostic performance. The findings provide a potential biomarker network for clinical applications and further validation is needed.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
