Article
Dentistry, Oral Surgery & Medicine
X. Lin, Q. Li, L. Hu, C. Jiang, S. Wang, X. Wu
Summary: The study reveals the crosstalk between dental pulp and dental follicle through the OGN-Hh pathway, which regulates the stemness and multidirectional differentiation of dental follicle stem cells. Ogn(-/-) mice exhibit accelerated root growth rate and thicker hard tissues in the root, suggesting the activation of the Hh pathway and increased cell proliferation and differentiation. This study provides important insights into the molecular regulation of early periodontal tissue development and lays a theoretical foundation for tooth root biology and periodontal tissue regeneration research.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Physiology
Yue Zhang, Hao Zhang, Guohua Yuan, Guobin Yang
Summary: The study reveals that the circRNA circKLF4 in mice promotes odontoblastic differentiation by acting as a sponge for miRNA-1895 and miRNA-5046.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Jing Pan, Qianhui Ren, Zhao Yang, Ying Guo, Kubin Kwon, Checheng Shen, Yueying Wang, Fang Ji
Summary: Melatonin plays a regulatory role in the cell number, differentiation, and mineralization of ALCs. It limits the cell number of ALCs and promotes the production of actin fibers and mRNA levels of enamel matrix proteins. Analysis of RNA-seq data suggests the involvement of Wnt signaling pathway in the differentiation of ALCs under the influence of melatonin.
SCIENTIFIC REPORTS
(2022)
Review
Cell Biology
Mitsutoshi Kimura, Hajime Moteki, Masahiko Ogihara
Summary: We investigated the effects of various growth factors, cytokines, hormones, neurotransmitters, and autacoids on the proliferation of hepatocytes using in vitro primary cultured hepatocytes. Multiple indicators including changes in DNA synthesis, nuclear number, cell number, cell cycle, and gene expression were examined. We also analyzed the intracellular signaling pathways and identified several factors that promote hepatocyte proliferation, such as HGF, EGF, TGF-alpha, IL-1 beta, TNF-alpha, insulin, growth hormone (GH), prostaglandin (PG), and serotonin (5-HT).
Article
Cell Biology
Jiangyi Wang, Xiaoyu Lin, Zongshan Shen, Guoqing Li, Lei Hu, Qiong Li, Yang Li, Jinsong Wang, Chunmei Zhang, Songlin Wang, Xiaoshan Wu
Summary: Epithelial-mesenchymal interactions via the PI3K/AKT signaling pathway play a crucial role in tooth development and odontoblast differentiation.
Article
Endocrinology & Metabolism
Fuping Zhang, Shengyan Yang, Liulin Jiang, Jiawei Liu, Yifan He, Xinyue Sheng, Haoling Chen, Jun Kang, Shilin Jia, Wenguo Fan, Fang Huang, Hongwen He
Summary: Tooth development is regulated by circadian rhythm, and melatonin (MT) plays a significant role in dentin formation and odontoblastic differentiation. This study explored how MT regulates odontoblastic differentiation through mitochondrial dynamics. The researchers found that MT enhances odontoblastic differentiation by promoting mitochondrial fusion, and identified malic enzyme 2 (ME2) as the key downstream effector of MT that regulates mitochondrial function and dentin formation.
JOURNAL OF PINEAL RESEARCH
(2023)
Article
Cell & Tissue Engineering
Ning Li, Yichi Zhang, Narayan Nepal, Guoqing Li, Ningning Yang, Haoyuan Chen, Qiuchi Lin, Xuechun Ji, Sijia Zhang, Shizhu Jin
Summary: The study demonstrated that HGF-DPSCs can alleviate intestinal mucosa injuries by transdifferentiating into ISC-like cells, promoting ISC-like cell proliferation, suppressing inflammatory responses, and reducing oxidative stress damage, which offers new insights for the clinical treatment of UC.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Rheumatology
Anna Deminger, Eva Klingberg, Merja Nurkkala, Mats Geijer, Hans Carlsten, Lennart T. H. Jacobsson, Helena Forsblad-d'Elia
Summary: This study found that elevated baseline s-HGF levels were associated with the development of new syndesmophytes in male AS patients, but no association was found in females. Changes in s-HGF were positively correlated with changes in ESR and CRP.
Article
Multidisciplinary Sciences
Song-Chang Lin, Guoyu Yu, Yu-Chen Lee, Jian H. Song, Xingzhi Song, Jianhua Zhang, Theocharis Panaretakis, Christopher J. Logothetis, Yoshihiro Komatsu, Li-Yuan Yu-Lee, Guocan Wang, Sue-Hwa Lin
Summary: Metastatic prostate cancer leads to bone-forming lesions, which are derived from endothelial cells that undergo EC-to-osteoblast transition. This study shows that EC-to-osteoblast transition also occurs during normal bone formation. Using a dual-color reporter mouse model, the researchers observe this transition in both endochondral and intramembranous bone formation. RNA sequencing reveals the upregulation of GATA family transcription factors in EC-osteoblast hybrid cells, and inhibition of GATA3 inhibits BMP4-induced mineralization. These findings suggest a new understanding of the endothelial origin of osteoblasts.
Article
Multidisciplinary Sciences
Chong Fu, Yan Chen, Wei Xu, Yanping Zhang
Summary: Using genetic data, a Mendelian randomization analysis was conducted to explore the causal relationships between inflammatory cytokines and migraine. The results showed a positive association between hepatocyte growth factor (HGF) and migraine risk, while interleukin-2 (IL-2) was considered a downstream consequence of migraine.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Wesuk Kang, Soyoon Park, Dabin Choi, Bomin Son, Taesun Park
Summary: This study revealed the promotion of cell proliferation by alpha-phellandrene in a concentration-dependent manner in DPCs. It also demonstrated the effects of alpha-phellandrene on cAMP downstream effectors and its selective upregulation of VEGF expression. These findings uncover the potential of alpha-phellandrene in preventing hair loss.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Haoling Chen, Jun Kang, Fuping Zhang, Tong Yan, Wenguo Fan, Hongwen He, Fang Huang
Summary: SIRT4 expression increases during odontogenic differentiation, with knockdown leading to reduced differentiation and mineralization, while overexpression has the opposite effect. SIRT4 regulates mitochondrial functions and antioxidant capacity of DPCs, impacting dentin formation and tooth development.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2021)
Article
Cell & Tissue Engineering
Yue Zhang, Hao Zhang, Ziqiu Xiao, Guohua Yuan, Guobin Yang
Summary: In this study, IPO7 was found to promote odontoblastic differentiation and inhibit osteoblastic differentiation by regulating RUNX2 expression and translocation differently.
Review
Neurosciences
JoAnn Buchanan, Nuno Macarico da Costa, Lucas Cheadle
Summary: Oligodendrocyte precursor cells (OPCs) are non-neuronal brain cells that give rise to oligodendrocytes, glia that myelinate the axons of neurons in the brain. They are not only involved in myelination but also play diverse roles in the nervous system, including blood vessel formation and antigen presentation. Emerging literature suggests that OPCs are essential for establishing and remodeling neural circuits in the brain, and they have specialized features that allow them to integrate activity-dependent and molecular cues for brain wiring. Studying the communication between neurons and glia, including OPCs, is important for understanding both health and disease.
TRENDS IN NEUROSCIENCES
(2023)
Article
Gastroenterology & Hepatology
Takeshi Katsuda, Hector Cure, Jonathan Sussman, Kamen P. Simeonov, Christopher Krapp, Zoltan Arany, Markus Grompe, Ben Z. Stanger
Summary: This study introduces a rapid in vivo multiplexed editing (RIME) method that enables the efficient inactivation of one or more genes in the mouse liver. The method is quick, efficient, and cost-effective, making it a valuable tool for analyzing multiple genes in vivo.
Article
Dentistry, Oral Surgery & Medicine
Yiyin Chen, Jiuhong Deng, Bin Li, Yang Yang, Zihan He, Ling Ye, Linglin Zhang, Qian Ren, Qinghua Zheng
Summary: Pre-clinical dental training in China faces challenges such as insufficient training time and facilities, with students lacking access to standard training and quality assurance. The application of VR technology in dental practice shows potential value, with students from higher-ranked schools showing more satisfaction and positive attitudes towards its use.
EUROPEAN JOURNAL OF DENTAL EDUCATION
(2022)
Review
Chemistry, Multidisciplinary
Peng Yu, Fanyuan Yu, Jie Xiang, Kai Zhou, Ling Zhou, Zhengmin Zhang, Xiao Rong, Zichuan Ding, Jiayi Wu, Wudi Li, Zongke Zhou, Ling Ye, Wei Yang
Summary: Rebuilding mineralized tissues in skeletal and dental systems is costly and challenging. Artificial scaffolds have emerged as efficient alternatives to autografts, allografts, and xenografts, which have limited sources and carry significant risks. This article systematically summarizes the strategies of cell-free biomimetic mineralization (BM) and cell-dependent scaffolds in regenerative dentistry and orthopedics, focusing on their mechanisms. By understanding the mechanisms, researchers can make better clinical choices and approach research directions for rebuilding hard tissues.
ADVANCED MATERIALS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Shuang Wang, Xiuyou Wang, Jinhong Jiang, Sanjay Kumar Tiwari, Yao Xiao, Ling Ye, Li Peng
Summary: This study aimed to investigate the relationship between the surgical access line (SAL) of maxillary posterior teeth and the maxillary sinus floor (MSF) in endodontic microsurgery using cone-beam computed tomographic imaging. The scans of 190 subjects were evaluated, and it was found that the distance between the SAL and MSF increased with age. The results of this study provide important reference for surgeons to evaluate surgical difficulty and decrease the risk of maxillary sinus perforation.
JOURNAL OF ENDODONTICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Liu Wang, Xiangfen Li, Yao Song, Lan Zhang, Ling Ye, Xuedong Zhou, Dongzhe Song, Dingming Huang
Summary: This study explored the effect of BMP9 on the osteogenic differentiation of inflammatory periodontal ligament stem cells (PDLSCs). The results showed that BMP9 promoted osteogenic differentiation of PDLSCs, but also upregulated the expression of inflammatory markers. NELL1 played an important role in this process by activating the MAPK/p38/ERK signaling pathway, reducing the expression of inflammatory genes induced by BMP9, and enhancing the osteogenic effect of the cells.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Fanyuan Yu, Fengli Huo, Feifei Li, Yanqin Zuo, Chenglin Wang, Ling Ye
Summary: This study investigates the role of regenerative cells odontoblasts (ODs) in inflammatory mineral resorption and matrix degradation in dental internal resorption (IR). The study reveals that inflamed ODs have strong capabilities of matrix degradation and tissue resorption, and further elucidates the involvement of nuclear factor-kappa b (NF-kappa B) signaling in this process.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2022)
Article
Cell & Tissue Engineering
Boer Li, Yu Shi, Mengyu Liu, Fanzi Wu, Xuchen Hu, Fanyuan Yu, Chenglin Wang, Ling Ye
Summary: The study found that NAD(+)-mediated mitochondrial OXPHOS is essential for osteogenic commitment in BMSCs and bone repair, which may provide a potential therapeutic target for bone repair and regeneration.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hengyi Fu, Xiujun Tan, Ling Ye, Chenglin Wang
Summary: The study demonstrated that Wnt6 regulates human dental papilla cell differentiation by activating the beta-catenin signaling pathway. Wnt6 inhibited differentiation in vitro but promoted mineralization in vivo, similar to Wnt3a but at a lower level. The potential mechanism of Wnt6-regulated cell differentiation is through the activation of the Wnt beta-catenin signaling pathway.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yujie Hu, Chengjia You, Chenghao Song, Yu Shi, Ling Ye
Summary: The study revealed that O-GlcNAcylation modification is involved in regulating the differentiation and mineralization of dental pulp cells, playing a crucial role in the mTORC1 pathway, and the O-GlcNAc modification of RAPTOR is essential for inducing odontogenesis.
ARCHIVES OF ORAL BIOLOGY
(2022)
Review
Cell Biology
Qingge Ma, Chenghao Song, Bei Yin, Yu Shi, Ling Ye
Summary: This review summarizes the components and functions of TrxG complexes, and provides an overview of the regulation mechanisms of TrxG in MSCs osteogenic and chondrogenic differentiation. Additionally, the prospects of epigenetic regulation mediated by TrxG in bone and cartilage regeneration are discussed.
CELL PROLIFERATION
(2022)
Article
Multidisciplinary Sciences
Fanyuan Yu, Feifei Li, Liwei Zheng, Ling Ye
Summary: This study reveals the importance of epigenetic regulation through Ezh2/H3K27me3 in determining the fate of epithelial stem cells (ASCs). It plays a crucial role in maintaining tissue homeostasis and facilitating tissue regeneration.
Article
Chemistry, Multidisciplinary
Yimin Sun, Shengdong Mu, Zhenyu Xing, Jiusi Guo, Zihe Wu, Fanyuan Yu, Mingru Bai, Xianglong Han, Chong Cheng, Ling Ye
Summary: It is important to explore high-efficiency ROS-elimination materials for combating oxidative stress in various diseases, especially in stem-cell-based biotherapeutics. This study reports an innovative concept of designing ROS-elimination artificial biocatalysts with Ru catalytic centers, which mimic the Fe-N active centers of natural catalase. The results show that the artificial biocatalysts with Ru cluster centers have exceptional ROS-elimination activity and can provide efficient protection for stem cells in high ROS level conditions.
ADVANCED MATERIALS
(2022)
Article
Biochemistry & Molecular Biology
Sha Leng, Linyi Liu, Weizhe Xu, Fan Yang, Jing Du, Ling Ye, Dingming Huang, Lan Zhang
Summary: The key to preventing pulp necrosis in the early stage of pulpitis is to promote tissue repair through cell migration. Our study found that inflammation suppresses the expression of SDF-1 alpha, a molecule that promotes cell migration. This suppression is achieved through up-regulation of TNFR2 and AIP1, which activate the JNK/c-Jun (Ser63) pathway.
Review
Cell Biology
Liuyan Huang, Feifei Li, Ling Ye, Fanyuan Yu, Chenglin Wang
Summary: Dynamic chromatin accessibility controls gene expression, regulating stem cell fate determination and tissue homeostasis. Polycomb repressive complex 2 (PRC2), as a histone-modifying enzyme that predominantly mediates methylation of lysine 27 in histone H3 (H3K27me1/2/3), plays a canonical role in targeting developmental regulators during stem cell differentiation. Embryonic ectoderm development (EED), the core scaffold subunit of PRC2 and an H3K27me3-recognizing protein, is involved in PRC2 stabilization and allosterically stimulated PRC2. Experimental data suggest that EED-associated epigenetic modifications are essential for stem cell maintenance and differentiation. This review discusses the structural architecture of EED, its contributions, and underlying mechanisms in mediating lineage differentiation of different stem cells during epigenetic modification to enhance our understanding of PRC2.
CELL PROLIFERATION
(2023)
Article
Engineering, Biomedical
Yanlin Wu, Xuan Li, Yimin Sun, Xiujun Tan, Chenglin Wang, Zhenming Wang, Ling Ye
Summary: By introducing trace amounts of additives, the mechanical properties of biomimetic hydrogels were improved. The hydrogels were functionalized with biocompatible, antioxidant, and growth factor-loaded nanoparticles, which enhanced their osteoinductive capacity. Animal experiments showed that the functionalized hydrogels promoted bone regeneration and had potential as bone regeneration materials.
BIOACTIVE MATERIALS
(2023)
Article
Materials Science, Biomaterials
Xiujun Tan, Jiayi Wu, Rui Wang, Chenglin Wang, Yimin Sun, Zhenming Wang, Ling Ye
Summary: The repair of large bone defects is an urgent problem in the clinic and can be hindered by redox homeostasis disruption. This study presents a biodegradable hydrogel with antioxidant and osteogenic capabilities, which can enhance osteo-related gene expression and eliminate intracellular ROS levels, promoting bone regeneration. The findings provide new insights into the designing of regenerative bone grafts with enhanced osteogenic and antioxidant capabilities.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
