Article
Medicine, Research & Experimental
Mohamed M. Elashiry, Shereen N. Raafat, Franklin R. Tay, Shehabeldin M. Saber
Summary: The study found that rapamycin has a positive effect on the viability and osteogenic differentiation potential of human periodontal ligament stem cells (hPDLSCs) in the presence of sodium hypochlorite (NaOCl). The hPDLSCs treated with rapamycin showed the highest percentage of viable cells and the best osteogenic differentiation potential. Rapamycin achieves these effects by activating autophagy and inhibiting mTOR gene expression.
Article
Engineering, Biomedical
Zain Siddiqui, Amanda M. Acevedo-Jake, Alexandra Griffith, Nurten Kadincesme, Kinga Dabek, Dana Hindi, Ka Kyung Kim, Yoshifumi Kobayashi, Emi Shimizu, Vivek Kumar
Summary: Pulp capping and dental pulp regeneration are evolving techniques to regenerate pulp tissue and maintain tooth vitality, with various methods and materials being used in regenerative endodontics.
BIOACTIVE MATERIALS
(2022)
Article
Dermatology
Lixuan Wu, Shan Jiang, Huan Ge, Zhiyu Cai, Xiaojing Huang, Chengfei Zhang
Summary: The study evaluated the effect of optimized irrigation with photon-induced photoacoustic streaming (PIPS) activation of different irrigants on smear layer removal, dentin microhardness, attachment morphology, and survival of stem cells of the apical papilla (SCAP). The results showed that irrigation optimized with PIPS activation of EDTA was conducive to smear layer removal and SCAP viability. No significant differences were found in other groups when optimized with PIPS (distilled water or EDTA), indicating the effectiveness of PIPS activation of EDTA for irrigation.
LASERS IN SURGERY AND MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Sahng G. Kim
Summary: Despite the recent surge in studies on dental pulp regeneration using tissue engineering strategies, the translation of these findings into therapeutic applications remains a challenge. This scoping review systematically reviewed the efficacy of mesenchymal stem cell transplantation for dental pulp regeneration, showing potential for vascularized pulp-like tissue regeneration and successful regeneration of vital tissue in clinical trials. More high-quality studies are needed to further evaluate the safety and efficacy of stem cell transplantation for dental pulp regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ruei-Hong Lin, Hsu-Tung Lee, Chun-An Yeh, Yi-Chin Yang, Chiung-Chyi Shen, Kai-Bo Chang, Bai-Shuan Liu, Hsien-Hsu Hsieh, Hui-Min David Wang, Huey-Shan Hung
Summary: The research shows that using AuNPs at concentrations of 1.25 and 2.5 ppm can enhance cell proliferation, reduce reactive oxygen species generation, and attenuate platelet/monocyte activation. AuNPs can also promote cell adhesion, increase the expression of matrix metalloproteinase (MMP-2/9), decrease cell apoptosis and the release of inflammatory cytokines. Animal model experiments demonstrate that treating with 1.25 and 2.5 ppm AuNPs exhibits better biocompatibility and endothelialization. This suggests that AuNPs can be used as a biocompatible nanomedicine for regeneration research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Vincenzo Tosco, Riccardo Monterubbianesi, Jose Aranguren, Lucia Meme, Angelo Putignano, Giovanna Orsini
Summary: Irrigation is an important step in endodontics for bacteria disinfection and smear layer removal. This study compared four different irrigation systems and found that Group D had the greatest efficacy on smear layer removal.
APPLIED SCIENCES-BASEL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Diana B. Sequeira, Ana Rafaela Oliveira, Catarina M. Seabra, Paulo J. Palma, Carlos Ramos, Maria H. Figueiredo, Ana C. Santos, Ana L. Cardoso, Joao Peca, Joao Miguel Santos
Summary: The findings of this study demonstrate that SCAPs combined with PRP scaffold can induce the formation of dentin-like and pulp-like tissues in root segments. The presence of barriers such as ProRoot MTA or Biodentine did not significantly affect the deposition of hard material in the root segments, but the area of newly deposited dentin was significantly greater in segments with a Biodentine barrier compared to a ProRoot MTA barrier.
CLINICAL ORAL INVESTIGATIONS
(2021)
Article
Biochemistry & Molecular Biology
Martyna Smeda, Kerstin M. Galler, Melanie Woelflick, Andreas Rosendahl, Christoph Moehle, Beate Lenhardt, Wolfgang Buchalla, Matthias Widbiller
Summary: Both dental pulp and apical papilla are potential sources of stem cells for regenerative endodontic treatments. This study compared the molecular characteristics of dental pulp stem cells (DPSC) and stem cells from the apical papilla (SCAP). The results showed that both cell types expressed similar mesenchymal stem cell markers and had the ability to differentiate into various lineages. However, differences were observed in the profiles of secreted molecules and gene regulation. Overall, both DPSC and SCAP are suitable for dental pulp tissue engineering from a molecular biological perspective.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Paper & Wood
Katarina Kacvinska, Martina Travnickova, Lucy Vojtova, Petr Polacek, Jana Dorazilova, Martin Kohoutek, Kristyna Hlinakova, Julia Pajorova, Maria Tirpakova, Lucie Bacakova
Summary: This study compares the performance of two types of bioresorbable cellulose in composite scaffolds. The calcium-sodium salt of oxidized cellulose significantly improves the stiffness and elasticity of collagen fibrils, while the sodium salt of carboxymethylcellulose reduces these properties. The addition of oxidized cellulose improves cell-material interaction and enhances the adhesion, proliferation, and metabolic activity of human adipose-derived stem cells. In contrast, carboxymethylcellulose only provides limited mechanical support for cells and inhibits their attachment and proliferation.
Article
Dentistry, Oral Surgery & Medicine
Ana Flavia Balestrero Cassiano, Heman Coaguila-Llerena, Cintia Silva Santos, Luana Raphael da Silva, Lucas Fabricio Bahia Nogueira, Pietro Ciancaglini, Gisele Faria
Summary: This study assessed the effects of OCT on stem cells from human dental pulp and apical papilla. The results showed that OCT promoted migration, proliferation, and alkaline phosphatase activity, which could be advantageous for regenerative endodontic procedures.
JOURNAL OF ENDODONTICS
(2022)
Review
Cell Biology
Dixin Cui, Sihan Yu, Xin Zhou, Ying Liu, Lu Gan, Yue Pan, Liwei Zheng, Mian Wan
Summary: Traditional apexification treatment for immature permanent teeth with pulp necrosis may lead to abnormal root morphology and compromised long-term prognosis. Regeneration endodontics procedures (REPs), including both cell-free and cell-based approaches, have shown promising potential in periapical lesion healing and continued root development. However, challenges remain in achieving full regeneration of the pulp-dentin complex.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Medicine, General & Internal
Alfredo Iandolo, Massimo Pisano, Dina Abdellatif, Giuseppe Sangiovanni, Giuseppe Pantaleo, Stefano Martina, Alessandra Amato
Summary: This study compares the smear layer removal between the 3D cleaning technique and traditional syringe needle irrigation. The 3D cleaning technique showed better results in removing debris and smear layers compared to the traditional method.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cell Biology
Koyo Takimoto, Matthias Widbiller, Anibal Diogenes
Summary: Research has shown that dental infections can interfere with the differentiation of stem cells, causing different cellular fates and promoting macrophage migration through the upregulation of chemokines. Activation of TLR3 inhibits osteogenic and odontoblastic differentiation, which can be reversed by inhibiting or targeting TLR3 expression.
Article
Dentistry, Oral Surgery & Medicine
Juliana S. Ribeiro, Carolina K. Sanz, Eliseu A. Munchow, Nikhil Kalra, Nileshkumar Dubey, Carlos Enrique C. Suarez, J. Christopher Fenno, Rafael G. Lund, Marco C. Bottino
Summary: This study aimed to develop photocrosslinkable CHX/GelMA hydrogels with broad spectrum anti-bacterial action as a clinically viable cell-friendly disinfection therapy for regenerative endodontics.
Article
Dentistry, Oral Surgery & Medicine
Fang-Chi Li, Alireza Shahin-Shamsabadi, P. Ravi Selvaganapathy, Anil Kishen
Summary: This study developed and characterized a 3D tissue construct for studying the interactions between SCAPs and macrophages in proinflammatory and anti-inflammatory environments. The results showed distinct interactions between SCAPs and macrophages in the tissue constructs, and the growth of cells and cell-to-cell and cell-to-matrix interactions resulted in a change in the tissue graft's dimensions.
JOURNAL OF ENDODONTICS
(2022)
Article
Dentistry, Oral Surgery & Medicine
Paul J. Feldon, Peter E. Murray, James G. Burch, Malcolm Meister, Matthew A. Freedman
AMERICAN JOURNAL OF ORTHODONTICS AND DENTOFACIAL ORTHOPEDICS
(2010)
Article
Cell Biology
Kiranmai Chadipiralla, Ji Min Yochim, Bindu Bahuleyan, Chun-Yuh Charles Huang, Franklin Garcia-Godoy, Peter E. Murray, Eric J. Stelnicki
CELL AND TISSUE RESEARCH
(2010)
Article
Dentistry, Oral Surgery & Medicine
Ghaeth H. Yassen, Tien-Min G. Chu, Maxime A. Gallant, Matthew R. Allen, Mychel M. Vail, Peter E. Murray, Jeffrey A. Platt
CLINICAL ORAL INVESTIGATIONS
(2014)
Review
Dentistry, Oral Surgery & Medicine
Franklin Garcia-Godoy, Peter E. Murray
DENTAL TRAUMATOLOGY
(2012)
Article
Dentistry, Oral Surgery & Medicine
Judith Lubin, Maria A. Hernandez, Saulius E. Drukteinis, William B. Parker, Peter E. Murray
Article
Dentistry, Oral Surgery & Medicine
M. A. Elseed, P. E. Murray, F. Garcia-Godoy, K. N. Namerow
INTERNATIONAL ENDODONTIC JOURNAL
(2009)
Article
Dentistry, Oral Surgery & Medicine
Faustino Garcia, Peter E. Murray, Franklin Garcia-Godoy, Kenneth N. Namerow
JOURNAL OF APPLIED ORAL SCIENCE
(2010)
Article
Dentistry, Oral Surgery & Medicine
Matthew Gebhardt, Peter E. Murray, Kenneth N. Namerow, Sergio Kuttler, Franklin Garcia-Godoy
JOURNAL OF ENDODONTICS
(2009)
Article
Dentistry, Oral Surgery & Medicine
Ingrid Epelman, Peter E. Murray, Franklin Garcia-Godoy, Sergio Kuttler, Kenneth N. Namerow
JOURNAL OF ENDODONTICS
(2009)
Article
Dentistry, Oral Surgery & Medicine
Cameron Howard, Peter E. Murray, Kenneth N. Namerow
JOURNAL OF ENDODONTICS
(2010)
Article
Dentistry, Oral Surgery & Medicine
Sheila Chandrahasa, Peter E. Murray, Kenneth N. Namerow
JOURNAL OF ENDODONTICS
(2011)
Article
Dentistry, Oral Surgery & Medicine
Christine Manguno, Peter E. Murray, Cameron Howard, Jonathan Madras, Stephen Mangan, Kenneth N. Namerow
JOURNAL OF ENDODONTICS
(2012)
Letter
Dentistry, Oral Surgery & Medicine
Cameron Howard, Stephen Mangan, Jeff Hanzon
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION
(2009)
Review
Dentistry, Oral Surgery & Medicine
V. Nagendrababu, P. E. Murray, R. Ordinola-Zapata, O. A. Peters, I. N. Rocas, J. F. Siqueira, E. Priya, J. Jayaraman, S. J. Pulikkotil, P. M. H. Dummer
INTERNATIONAL ENDODONTIC JOURNAL
(2019)
Review
Biotechnology & Applied Microbiology
Peter E. Murray
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2018)
