Journal
JOURNAL OF ENDODONTICS
Volume 34, Issue 8, Pages 939-944Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2008.05.015
Keywords
dental pulp cells; migration; proliferation; stromal cell-derived factor-1 alpha-CXCR4 axis; tissue repair
Categories
Funding
- NIDCR NIH HHS [R01 DE011442, DE11442, R01 DE011442-06A2] Funding Source: Medline
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Recent reports have suggested that the stromal cell-derived factor (SDF)-1 alpha-CXCR4 axis has a direct effect on stem and progenitor cell recruitment in muscle and neural tissue repair after injury. No information is available about SDF-1 alpha or CXC chemokine receptor 4 (CXCR4) in dental tissues. The aim of this study was to assess the expression of SDF-1 alpha and its receptor, CXCR4, in healthy or inflamed human dental pulp and to evaluate the effects of SDF-1 alpha on dental pulp cells (DPCs) in both proliferation and migration in vitro. Immunohistochemical staining and reverse-transcription polymerase chain reaction detected weak expression of SDF-1 alpha and CXCR4 in healthy dental pulp and strong expression of SDF-1 alpha and CXCR4 in inflamed dental pulp. A methylthiazol tetrazolium assay showed that SDF-1 alpha could not promote DPCs proliferation. A transmigration assay, however, indicated that SDF-1 alpha enhanced DPCs migration, which could be abolished by anti-CXCR4 antibodies. Taken together, these results imply that the SDF-1 alpha-CXCR4 axis may play a role in the recruitment of CXCR4-positive DPCs toward the damaged sites.
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