Journal
JOURNAL OF ENDODONTICS
Volume 34, Issue 8, Pages 945-949Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.joen.2008.05.011
Keywords
alternatively activated monocytes/macrophages; calcitonin gene-related peptide; CD163; CGRP-receptor; irreversible pulpitis; neuropeptides
Categories
Ask authors/readers for more resources
The purpose of this study was to quantify the percentage and the mean fluorescence intensity of viable alternatively activated monocytes/macrophages (AAM phi) CD163+ positive for calcitonin gene-related peptide receptor (CGRPr) within the total AAM phi population in human dental pulp. Pulp tissue samples were collected from teeth with a clinical diagnosis of irreversible pulpitis (n = 13), pulps with induced inflammation (n = 13), and normal pulps (n = 13). All samples were labeled to identify positive cells for CGRPr and CD163 using a flow cytometry assay. Results demonstrated that a high percentage of total viable AAM phi CD163+ expressed CGRPr on their membranes (72.12% in healthy pulp, 62.20% in irreversible pulpitis, and 58.01% in induced pulpitis). Significant differences were found between mean AAMo CD163+ fluorescence for CGRPr according to pulp condition, being greater in irreversible pulpitis. it can be concluded that AAMo CD163+ are expressed during normal and inflammatory processes, supporting the hypothesis that they could exercise an anti-inflammatory action that could be controlled by CGRP signaling after its binding.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available