Article
Biochemistry & Molecular Biology
Yoshiaki Soejima, Nahoko Iwata, Ran Nishioka, Mako Honda, Yasuhiro Nakano, Koichiro Yamamoto, Atsuhito Suyama, Fumio Otsuka
Summary: Orexins play important roles in sleep-wake regulation and food intake in the central nervous system. This study investigated the functions of orexin in adrenal steroidogenesis and its interaction with bone morphogenetic proteins (BMPs). The results suggest that both orexin and BMP-6 accelerate adrenocortical steroidogenesis in human adrenocortical cells, and these pathways mutually inhibit each other.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Ye Yang, Chunlei Wang, Hong Shen, Hongliang Fan, Jing Liu, Nanxiang Wu
Summary: This study investigated the effects of cis-bifenthrin on the biosynthesis of adrenal steroids in human adrenocortical carcinoma cells. It was found that cis-bifenthrin can decrease the production of cortisol and aldosterone by disrupting cAMP signaling pathway.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Cell Biology
Hana Duranova, Veronika Fialkova, Veronika Valkova, Jana Bilcikova, Lucia Olexikova, Norbert Lukac, Peter Massanyi, Zuzana Knazicka
Summary: Cell lines are important tools for scientific research, particularly in the study of molecular pathways and gene expression. The NCI-H295R cell line is widely used to study the human adrenal steroidogenic pathway. This review provides information on the origin, development, physiological and ultrastructural characteristics of the NCI-H295R cell line, as well as its usage in studies related to steroidogenesis.
Article
Cell Biology
Michael Curley, Annalucia Darbey, Liza O'Donnell, Karen R. Kilcoyne, Kirsten Wilson, Will Mungall, Diane Rebourcet, Jingtao Guo, Rod T. Mitchell, Lee B. Smith
Summary: The LIFR signaling pathway negatively regulates steroidogenic enzyme expression and testosterone biosynthesis in testicular Leydig cells, providing new insights into the intricate regulation of LC steroidogenic function.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Environmental Sciences
Yuan Huang, Wei Zhang, Na Cui, Zhiming Xiao, Wenyu Zhao, Ruiguo Wang, John P. Giesy, Xiaoou Su
Summary: This study found that Fluorene-9-bisphenol (BHPF) inhibits steroidogenesis in human adrenocortical carcinoma cells (H295R), possibly through the modulation of cellular signaling pathways and the down-regulation of steroidogenic factor-1 (SF-1) protein. These findings contribute to a better understanding of the molecular mechanisms underlying BHPF-induced effects on steroidogenesis.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2022)
Article
Endocrinology & Metabolism
Yusuf Ali, Elise P. Gomez-Sanchez, Celso E. Gomez-Sanchez
Summary: The mTOR signaling pathway plays a crucial role in transducing the AngII signal initiating aldosterone and cortisol synthesis in HAC15 cells.
Article
Toxicology
Masahiro Ogawa, Junya Kitamoto, Takeo Takeda, Masami Hori, Kisako Shikano, Amami Yamanaka-Tanaka, Tomoki Tanaka, Tomoya Kawaguchi, Megumi Terada, Taku Tanaka
Summary: Sex hormones are primarily produced in the gonads and adrenal cortex. Endocrine-disrupting chemicals (EDCs) mimic, block, or interfere with hormones in the endocrine systems. Several assays are used to identify the disruption potential of nuclear receptors, with the H295R steroidogenesis assay being the only one detecting steroidogenesis disruption. To establish a short-term screening method for steroidogenesis disruption, rats were fed forskolin, prochloraz, or D5 for 14 days. The results emphasize the importance of comprehensively assessing the endocrine-disrupting effects of chemicals on steroidogenesis in vivo.
JOURNAL OF APPLIED TOXICOLOGY
(2023)
Article
Engineering, Chemical
Nikola Knizatova, Hana Greifova, Katarina Tokarova, Tomas Jambor, Lukasz J. Binkowski, Norbert Lukac
Summary: Recent research has shown that bisphenol analogues like BPB, BPF, and BPS can potentially affect the secretion of progesterone and testosterone in adrenal carcinoma cells, as well as impact cellular mitochondrial, lysosomal, and metabolic activity. Further detailed and systematic research is needed to fully understand the risks associated with the effects of bisphenols on steroidogenesis.
Article
Cell Biology
Yuka Sakata, Keren Cheng, Michinori Mayama, Yasunari Seita, Andrea J. Detlefsen, Clementina A. Mesaros, Trevor M. Penning, Kyosuke Shishikura, Wenli Yang, Richard J. Auchus, Jerome F. Strauss III, Kotaro Sasak
Summary: In this study, the researchers were able to recapitulate the development of human fetal adrenal cortex and generate fetal zone adrenal-cortex-like cells through stepwise induction of human pluripotent stem cells. They also identified key regulatory factors and signaling pathways involved in this process. These findings provide a framework for understanding human adrenocortical development and have implications for cell-based therapies of adrenal insufficiency.
DEVELOPMENTAL CELL
(2022)
Article
Biochemistry & Molecular Biology
Rona Karahoda, Sampada Kallol, Michael Groessl, Edgar Ontsouka, Pascale Anderle, Christa Fluck, Frantisek Staud, Christiane Albrecht
Summary: This study investigates the gestational age-dependent changes in the gene expression of key steroidogenic enzymes in human placentas, as well as explores the role of trophoblast cells in steroid biosynthesis and metabolism. The research provides insights into the cellular origin of steroidogenesis in the human placenta and highlights the functional activity of steroidogenic enzymes in primary human trophoblasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lauriane Relav, Mabrouka Doghman-Bouguerra, Carmen Ruggiero, Joao C. D. Muzzi, Bonald C. C. Figueiredo, Enzo Lalli
Summary: Steroidogenic factor-1 (SF-1) is a nuclear receptor transcription factor that regulates adrenal and gonadal development and function. It is involved in various processes including steroid synthesis and cell survival. SF-1 expression is restricted to the hypothalamic-pituitary axis and steroidogenic organs. Changes in SF-1 expression can lead to disorders in organogenesis and function, and SF-1 overexpression is associated with adrenal cortex carcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Yoshiaki Soejima, Nahoko Iwata, Yasuhiro Nakano, Koichiro Yamamoto, Atsuhito Suyama, Takahiro Nada, Hiroko Ogawa, Fumio Otsuka
Summary: Results indicate that Clock mRNA expression, regulated by FSK and inhibited by BMP-6 and activin, is closely linked to StAR expression in human adrenocortical cells.
Article
Cell Biology
Marie-Christin Jager, Melanie Patt, Victor Gonzalez-Ruiz, Julien Boccard, Tim Wey, Denise Winter, Serge Rudaz, Alex Odermatt
Summary: This study evaluated a novel approach to detect disturbances of steroidogenesis in H295R cells, exemplified by prochloraz and five anabolic steroids. The results show that chemical grouping and calculation of product/substrate ratios can provide valuable information on mode-of-action and help prioritize further experimental work.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2023)
Article
Endocrinology & Metabolism
Kai Otsuka, Shin Matsubara, Akira Shiraishi, Natsumi Takei, Yui Satoh, Miho Terao, Shuji Takada, Tomoya Kotani, Honoo Satake, Atsushi P. Kimura
Summary: The newly discovered testis-specific lncRNA, Start, is involved in testicular steroid activation, and its knockout leads to reduced testosterone levels, affecting spermatogenesis, but does not show significant abnormalities in adult mice.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hye-Rin Park, Hee-Jung Choi, Bo-Sung Kim, Tae-Wook Chung, Keuk-Jun Kim, Jong-Kil Joo, Dongryeol Ryu, Sung-Jin Bae, Ki-Tae Ha
Summary: The active compound paeoniflorin in Paeonia lactiflora significantly improved embryo implantation rate and increased cell adhesion through upregulating the expression of leukemia inhibitory factor (LIF). This suggests that paeoniflorin might be a potent drug candidate for treating endometrial implantation failure by enhancing endometrial receptivity.
Article
Nutrition & Dietetics
Anni Lehtonen, Lauri Uusitalo, Seppo Auriola, Katri Backman, Seppo Heinonen, Leea Keski-Nisula, Markku Pasanen, Juha Pekkanen, Tomi-Pekka Tuomainen, Raimo Voutilainen, Sari Hantunen, Marko Lehtonen
Summary: The study found a significant correlation between maternal dietary caffeine intake during the last trimester and caffeine content in newborn hair. Older, multiparous, overweight women, and smokers had the highest levels of caffeine in their maternal diet and in their newborn babies' hair.
EUROPEAN JOURNAL OF NUTRITION
(2021)
Review
Endocrinology & Metabolism
Satu Seppa, Tanja Kuiri-Hanninen, Elina Holopainen, Raimo Voutilainen
Summary: Primary amenorrhea is the absence of menstruation in females past a certain age, with various disorders in the hypothalamus-pituitary-ovarian axis as potential causes. Treatment goals depend on the underlying cause and individual patient, with fertility possible with timely etiological diagnostics even in cases without curative treatment options.
EUROPEAN JOURNAL OF ENDOCRINOLOGY
(2021)
Article
Endocrinology & Metabolism
Tero Varimo, Paivi J. Miettinen, Tiina Laine, Pia Salonen, Sirpa Tenhola, Raimo Voutilainen, Hanna Huopio, Matti Hero, Taneli Raivio
Summary: This study found that CHH patients had lower trabecular BMD at the distal radius and distal tibia compared to CDGP boys, despite being older, taller, and heavier. CHH males also had lower cortical thickness at the diaphyseal tibia compared to CDGP participants. In the CDGP group, serum testosterone levels correlated positively with trabecular BMD at the distal radius, and estradiol levels correlated positively with trabecular BMD at the distal tibia.
CLINICAL ENDOCRINOLOGY
(2021)
Article
Psychiatry
Aleksi Ruohomaki, Elena Toffol, Ville Airaksinen, Katri Backman, Raimo Voutilainen, Sari Hantunen, Tomi-Pekka Tuomainen, Jussi Lampi, Hannu Kokki, Ilona Luoma, Kirsti Kumpulainen, Seppo Heinonen, Leea Keski-Nisula, Juha Pekkanen, Markku Pasanen, Soili M. Lehto
Summary: Infants of mothers with elevated depressive symptoms postpartum in low- and lower-middle-income countries may have increased risk of somatic health problems during their first 12 months. These infants are more likely to experience abnormal crying, wheezing, gastroesophageal reflux, food allergy, and receive more analgesic medication. Adherence to vaccination schedules does not differ significantly between mothers with and without postpartum EDS.
JOURNAL OF PSYCHIATRIC RESEARCH
(2021)
Review
Oncology
Ville Paakinaho, Jorma J. Palvimo
Summary: Recent studies indicate that interactions between steroid receptors in breast and prostate cancers can lead to the rewiring of gene programs, altering biological processes and impacting disease progression.
ENDOCRINE-RELATED CANCER
(2021)
Article
Cell Biology
Gustavo Antonio Urrutia, Haribaskar Ramachandran, Pierre Cauchy, Kyungjin Boo, Senthilkumar Ramamoorthy, Soeren Boller, Esen Dogan, Thomas Clapes, Eirini Trompouki, Maria -Elena Torres-Padilla, Jorma J. Palvimo, Andrea Pichler, Rudolf Grosschedl
Summary: The study shows that SUMOylation of SATB2 in ESCs may play a key role in regulating pluripotency and differentiation by impacting gene silencing and activation, as well as higher-order chromatin interactions.
GENES & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
A. B. M. Kaiser Manjur, Joanna K. Lempiainen, Marjo Malinen, Markku Varjosalo, Jorma J. Palvimo, Einari A. Niskanen
Summary: BCOR has been identified as an essential interaction partner of GR, involved in the GC signaling pathway and affecting the expression of genes related to TNF signaling, GR signaling, and cell migration pathways. Depletion of BCOR, along with NCOR1, enhances cell migration and influences GR target gene expression in a combinatorial and gene-specific manner.
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Kaisa-Mari Launonen, Ville Paakinaho, Gianluca Sigismondo, Marjo Malinen, Reijo Sironen, Jaana M. Hartikainen, Hanna Laakso, Tapio Visakorpi, Jeroen Krijgsveld, Einari A. Niskanen, Jorma J. Palvimo
Summary: The study used ChIP-SICAP to uncover the chromatome composition around endogenous AR in CRPC cells, revealing the roles of SMARCA4 and SIM2 in chromatin accessibility and AR target gene expression. Depletion of SMARCA4 impacted a restricted set of AR target genes involved in cell morphogenetic changes, while silencing of SIM2 affected a larger group of androgen-regulated genes related to cellular responses and proliferation in CRPC cells.
Article
Endocrinology & Metabolism
Heta Huttunen, Tero Varimo, Hanna Huopio, Raimo Voutilainen, Sirpa Tenhola, Paivi J. Miettinen, Taneli Raivio, Matti Hero
Summary: In puberty promoting treatment, growth response is tightly correlated with serum testosterone level. Serum testosterone level is the best predictor of growth velocity in both treatment groups. In the Lz-group, adding serum oestradiol to the model significantly improved the growth estimate. Only boys with serum oestradiol above 10 pmol/L had a growth velocity above 8 cm/year.
CLINICAL ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sarah El Kharraz, Vanessa Dubois, Martin E. van Royen, Adriaan B. Houtsmuller, Ekatarina Pavlova, Nina Atanassova, Tien Nguyen, Arnout Voet, Roy Eerlings, Florian Handle, Stefan Prekovic, Elien Smeets, Lisa Moris, Wout Devlies, Claes Ohlsson, Matti Poutanen, Kevin J. Verstrepen, Geert Carmeliet, Kaisa-Mari Launonen, Laura Helminen, Jorma J. Palvimo, Claude Libert, Dirk Vanderschueren, Christine Helsen, Frank Claessens
Summary: Dimerization of the ligand-binding domain of the androgen receptor (AR) is crucial for transcriptional regulation and proper functioning of AR. Mutations disrupting LBD dimerization can lead to significant effects, as demonstrated in a mouse model in this study.
Article
Biochemistry & Molecular Biology
Tineke Vanderhaeghen, Steven Timmermans, Deepika Watts, Ville Paakinaho, Melanie Eggermont, Jolien Vandewalle, Charlotte Wallaeys, Lise Van Wyngene, Kelly Van Looveren, Louise Nuyttens, Sylviane Dewaele, Joke Vanden Berghe, Kelly Lemeire, Joey De Backer, Laura Dirkx, Wim Vanden Berghe, Guy Caljon, Bart Ghesquiere, Karolien De Bosscher, Ben Wielockx, Jorma J. Palvimo, Rudi Beyaert, Claude Libert
Summary: The study found that hypoxia impacts the hepatic response of glucocorticoid receptors to dexamethasone in mice, leading to three types of reprogramming of GR: weaker induction of classical GR-responsive genes, induction of specific genes in hypoxia, and activation of a group of genes by the HPA axis under hypoxia. Additionally, hypoxia-induced activation of the HPA axis is associated with GR-dependent lipolysis and ketogenesis.
Article
Chemistry, Analytical
Johanna Koskivuori, Raimo Voutilainen, Lauri Uusitalo, Marko Lehtonen, Timo Lakka, Seppo Auriola, Merja R. Hakkinen
Summary: Hair steroid analysis is a potential method to evaluate long-term steroid metabolism, especially in newborns and children. This study developed a quantitative and highly selective mass spectrometry method for simultaneous analysis of multiple steroids in human scalp hair.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Endocrinology & Metabolism
Annie M. Constable, Dimitris Vlachopoulos, Alan R. Barker, Sarah A. Moore, Sonja Soininen, Eero A. Haapala, Juuso Vaisto, Jarmo Jaeaeskelaeinen, Raimo Voutilainen, Seppo Auriola, Merja R. Hakkinen, Tomi Laitinen, Timo A. Lakka
Summary: This study aimed to investigate the mediation effect of various endocrine factors on the relationship between fat mass and bone mineral content (BMC) in children aged 9-11 years. The results showed a positive association between fat mass and BMC, which was influenced by different endocrine factors in girls and boys.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Sarah El Kharraz, Vanessa Dubois, Kaisa-Mari Launonen, Laura Helminen, Jorma J. Palvimo, Claude Libert, Elien Smeets, Lisa Moris, Roy Eerlings, Dirk Vanderschueren, Christine Helsen, Frank Claessens
Summary: Research suggests that the N/C interactions in the androgen receptor (AR) are not essential for the development of male phenotype under normal physiological conditions.
Article
Endocrinology & Metabolism
Tero Varimo, Paivi J. Miettinen, Kirsi Vaaralahti, Jorma Toppari, Hanna Huopio, Raimo Voutilainen, Sirpa Tenhola, Matti Hero, Taneli Raivio
Summary: LEAP2 levels decrease during puberty in boys, which is negatively correlated with growth rate and energy demand. Estradiol levels may play a permissive role in downregulating circulating LEAP2 levels.
JOURNAL OF THE ENDOCRINE SOCIETY
(2022)