Article
Chemistry, Physical
Jin Yang, Mingyong Zhou, Jiezhen Liu, Hao Wang, Can Weng
Summary: It was found that using alkanethiols SAMs to modify the Ni substrate could significantly reduce the surface adhesion of Ni substrate and lower the friction coefficient. Mixed alkanethiols SAMs exhibited better lubricity and wear resistance compared to SAMs of single component.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Maciej Sosna, Elena E. Ferapontova
Summary: The oriented coupling of redox enzymes to electrodes by reconstitution onto redox cofactors poses the requirements for a suitable self-assembled monolayer (SAM) design. The kinetics of electron transfer in binary SAMs composed of alkanethiols and hemin-conjugated linker molecules depends on the SAM composition and hemin surface density. The use of SAM-modified electrodes with loosely dispersed redox centers and rigid molecular wires is important for the effective on-surface reconstitution of proteins and enzymes.
Article
Multidisciplinary Sciences
Rajan Khadka, Ganpati Ramanath, Pawel Keblinski
Summary: Incorporating molecular nanolayers at inorganic interfaces offers promise for enhancing fracture energy, thermal and electrical transport. It is revealed that multilayering MNL-bonded inorganic interfaces can result in viscoelastic damping bandgaps. These findings provide possibilities for accessing and tuning novel dynamic mechanical responses in materials systems and devices.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Zixin Zhou, Wenting He, Hao Chao, Han Wang, Ping Su, Jiayi Song, Yi Yang
Summary: In this study, a specific hemin-based mimetic enzyme was synthesized by assembling zeolitic imidazolate framework-L (ZIF-L) and hemin. The obtained mimetic enzyme displayed enhanced peroxidase activity and resistance to severe conditions. It also served as a colorimetric sensor for uranium detection.
ANALYTICAL CHEMISTRY
(2022)
Article
Polymer Science
Xiaotong Fan, Ke Li, Siqi Liu, Tingting Wang, Yedong Ma, Zibiao Li, Chaobin He
Summary: Protein nanotubes were fabricated by coordinating imidazole-grafted horseradish peroxidase nanogels with Cu2+. The size of the nanotubes was adjustable by changing the amount of Cu2+ added, and a highly sensitive H2O2 detection system was established using these nanotubes. This work provides a facile method for constructing diverse and sophisticated functional protein nanomaterials.
Article
Chemistry, Multidisciplinary
Jing Liu, Taozhe Zhang, Jinyang Feng, Yue Cui, Li Zhang, Yunong Wang, Meiyu Cui, Donghao Li, Hulin Tai
Summary: It is discovered that N-acetylated microperoxidase-11 and G-quadruplex DNA can form a stable peptide-hemin/DNA hybrid-complex, and the peroxidase activity at the interface between hemin and the G-quartet planes exponentially increases with increasing K-a value.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Vivek M. Badiani, Samuel J. Cobb, Andreas Wagner, Ana Rita Oliveira, Sonia Zacarias, Ines A. C. Pereira, Erwin Reisner
Summary: This study elucidates the key factors governing the activity and stability of immobilized redox enzymes and the role of film loss, particularly hydrogen bonding, in stabilizing bioelectrode performance. Electrostatic interactions are responsible for enzyme orientation, while hydrogen bonding limits enzyme desorption and improves enzyme stability.
Article
Biophysics
Sara Bocanegra-Rodriguez, Carmen Molins-Legua, Pilar Campins-Falco, Fabien Giroud, Andrew J. Gross, Serge Cosnier
Summary: The non-covalent modification of carbon nanotube electrodes with pyrene derivatives is a versatile approach to enhance the electrical wiring of enzymes for biosensors and biofuel cells. The study found that pyrene-modified electrodes showed improved catalytic reduction with horseradish peroxidase (HRP), indicating great potential for biosensing and biofuel cell applications.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Guangming Li, Hao Liu, Tianding Hu, Fang Pu, Jinsong Ren, Xiaogang Qu
Summary: This study reports a dimensionality engineering strategy to convert conventional 2D Fe-N-4 centers into 3D structures by integrating oxidized sulfur functionalities onto the carbon plane. The results suggest that oxidized sulfur functionalities can serve as binding sites for assisting substrate orientation and facilitating the desorption of H2O, resulting in an outstanding specific activity of up to 119.77 U mg(-1), which is 6.8 times higher than that of conventional FeN4C SAzymes.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Rui Geng, Rui Chang, Qianli Zou, Guizhi Shen, Tifeng Jiao, Xuehai Yan
Summary: This study reports the construction of nanoassemblies through coassembly of an amphiphilic amino acid and hemin, which allows tailored peroxidase-mimicking activities. The optimized nanoassemblies exhibit remarkable catalytic efficiency and robustness in multiple catalytic cycles, and can be effectively integrated into a sensing system for catalytic detection of glucose.
Article
Chemistry, Physical
Qing Liu, Kaiwei Wan, Yingxu Shang, Zhen-Gang Wang, Yiyang Zhang, Luru Dai, Chen Wang, Hui Wang, Xinghua Shi, Dongsheng Liu, Baoquan Ding
Summary: Self-assembling, histidine-rich peptides are capable of catalyzing H2O2 reduction reactions through the formation of periodic arrays of histidine residues, similar to haem-dependent peroxidases. These findings may have implications for the design of advanced biomimetic catalytic materials.
Article
Chemistry, Multidisciplinary
Nemanja Cvjetan, Reinhard Kissner, Danica Bajuk-Bogdanovi, Gordana Ciric-Marjanovi, Peter Walde
Summary: This study investigates the template-assisted formation of conductive emeraldine salt form of polyaniline (PANI-ES) under mild and environmentally friendly conditions using hemin as a cost-effective catalyst, and SDS micelles as templates. The results demonstrate that the optimized reaction conditions yield PANI-ES-like products as the dominant species.
Article
Chemistry, Multidisciplinary
Nemanja Cvjetan, Lukas D. Schuler, Takashi Ishikawa, Peter Walde
Summary: Iron porphyrins, such as hemin, play important roles in biological systems and may have existed in early life forms. Hemin acts as a prosthetic group in heme peroxidases and catalyzes the oxidation of reducing substrates. The active site of heme peroxidases consists of a hydrophobic pocket in which hemin is embedded noncovalently. Experiments showed that certain additives can enhance the catalytic activity of hemin.
Article
Chemistry, Applied
Evelyn Ospina, Beatriz Alonso, Carmen M. Casado, M. Pilar Garcia Armada
Summary: The development of sensitive and rapid inhibition-based biosensors for monitoring trace levels of heavy metals is of high interest, and this study describes the fabrication of HRP-sensitive peroxide biosensors based on ferrocenyl thiolated DAB dendrimers. These biosensors utilize a bonding layer between electrodeposited and colloidal gold nanoparticles and show potential in detecting lead and copper ions. The electrochemical, kinetic, and analytical characterization of these biosensors are thoroughly investigated.
Review
Chemistry, Multidisciplinary
Limin Wang, Ulrich S. Schubert, Stephanie Hoeppener
Summary: Surface modification using self-assembled monolayers has seen significant advancement in the past decade, with the introduction of new chemical reactions such as nucleophilic substitution, click chemistry, supramolecular modification, photochemical reaction, and others, enhancing the properties of solid substrates like silicon and glass.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Electrochemistry
Maciej Sosna, Nelida Leiva-Eriksson, Leif Bulow, Elena E. Ferapontova
Article
Chemistry, Analytical
Dmitrii Pankratov, Mads Bendixen, Stepan Shipovskov, Ulrich Gosewinkel, Elena E. Ferapontova
ANALYTICAL CHEMISTRY
(2020)
Review
Chemistry, Analytical
Rimsha Binte Jamal, Stepan Shipovskov, Elena E. Ferapontova
Review
Chemistry, Analytical
Kamila Malecka, Edyta Mikula, Elena E. Ferapontova
Summary: The improvement in outcomes for various types of cancer in recent years is attributed to early tumor detection and widespread use of screening tests, with non-invasive, fast, and accurate diagnostic devices playing a crucial role. Biosensors utilizing aptamers in cancer diagnosis offer advantages such as in-vitro aptamer production, cost-effective chemical synthesis, and thermal stability, while electrochemical biosensing approaches provide sensitive, accurate, and affordable sensing methods. This review evaluates the design strategies and analytical performance of electrochemical aptamer-based biosensors for cancer diagnosis, highlighting their future prospects in cancer diagnostics.
Article
Nanoscience & Nanotechnology
Kamila Malecka, Elena E. Ferapontova
Summary: Thrombin is a central biomarker in cardiovascular diseases and Alzheimer's disease development, but its electroanalysis is complicated by low physiological concentrations and nonspecific binding. A new electrochemical method was developed to detect femtomolar levels of thrombin in serum and CSF using G4-hemin DNAzyme, which is simple, sensitive, and selective, providing a new tool for ultrasensitive electrocatalytic assays.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Electrochemistry
Naghmehalsadat Mirbagheri, Rui Campos, Elena E. Ferapontova
Summary: This study investigated the effect of different capping ligands and electrode substrates on the electrocatalysis of iridium oxide nanoparticles for water oxidation reaction. The results showed that the hydrophilic properties of the electrode substrate could affect the morphology and catalytic performance of the formed iridium oxide films.
Review
Chemistry, Physical
Elena E. Ferapontova
Summary: This article discusses the application of electrochemical methods in the study of neurodegenerative disorders, with a focus on recent advancements in bioelectroanalysis using aptamer- and antibody-modified electrodes.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Article
Chemistry, Analytical
Ana Diaz-Fernandez, Elena E. Ferapontova
Summary: This study introduces a simple and fast liquid biopsy method for analyzing the overexpressed HER-2/neu protein in aggressive cancers, which shows high sensitivity and low cost.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Lucia Gomez-Arconada, Ana Diaz-Fernandez, Elena E. Ferapontova
Summary: Serine protease thrombin is a strong neurotoxin and Alzheimer's disease biomarker. In this study, the O-2-dependent aptamer assay for thrombin was adapted to gold screen-printed electrodes (Au SPE) for point-of-care testing (POCT). The assay showed enhanced electrocatalytic activity in O2 reduction upon thrombin binding to the aptamer, and 0.5 fM thrombin was detected with a logarithmic linear range extending to 100 fM. The disposable aptasensor met basic POCT requirements but special surface pre-treatment and modification protocols were needed for practical applications.
Article
Chemistry, Multidisciplinary
Maciej Sosna, Elena E. Ferapontova
Summary: The oriented coupling of redox enzymes to electrodes by reconstitution onto redox cofactors poses the requirements for a suitable self-assembled monolayer (SAM) design. The kinetics of electron transfer in binary SAMs composed of alkanethiols and hemin-conjugated linker molecules depends on the SAM composition and hemin surface density. The use of SAM-modified electrodes with loosely dispersed redox centers and rigid molecular wires is important for the effective on-surface reconstitution of proteins and enzymes.
Article
Chemistry, Analytical
Ana Diaz-Fernandez, Alexey Ferapontov, Mikkel Holm Vendelbo, Elena E. Ferapontova
Summary: Researchers have developed a cellulase-linked sandwich bioassay on magnetic beads for detecting the levels of protein HER-2/neu in blood. This method is simple, low-cost, and enables fast and accurate liquid biopsy analysis.
ACS MEASUREMENT SCIENCE AU
(2023)
Article
Biochemistry & Molecular Biology
Tim J. Puchtler, Kerr Johnson, Rebecca N. Palmer, Emma L. Talbot, Lindsey A. Ibbotson, Paulina K. Powalowska, Rachel Knox, Aya Shibahara, Pedro M. S. Cunha, Oliver J. Newell, Mei Wu, Jasmin Chana, Evangelia-Nefeli Athanasopoulou, Andreas M. Waeber, Magdalena Stolarek, Ana-Luisa Silva, Justyna M. Mordaka, Michael Haggis-Powell, Christina Xyrafaki, James Bush, Ibrahim S. Topkaya, Maciej Sosna, Richard J. Ingham, Thomas Huckvale, Aurel Negrea, Boris Breiner, Justinas Slikas, Douglas J. Kelly, Alexander J. Dunning, Neil M. Bell, Mark Dethlefsen, David M. Love, Paul H. Dear, Jekaterina Kuleshova, Gareth J. Podd, Tom H. Isaac, Barnaby W. Balmforth, Cameron A. Frayling
NUCLEIC ACIDS RESEARCH
(2020)
Review
Chemistry, Physical
Elena E. Ferapontova
CURRENT OPINION IN ELECTROCHEMISTRY
(2020)