Review
Chemistry, Physical
Stephane Marinesco
Summary: Chemical transmission between neurosecretory cells is a central biological phenomenon that can be studied using electrochemical techniques. Carbon fiber microelectrodes are commonly used to detect neurotransmitters and can be modified for specific and sensitive in situ neurotransmitter detection, making them an essential tool for analyzing living cells and tissues.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Article
Chemistry, Analytical
Kobi P. Bermingham, Michelle M. Doran, Fiachra B. Bolger, John P. Lowry
Summary: The performance optimization of a polymer/enzyme composite biosensor for monitoring glutamate was conducted in vitro. The biosensor displayed appropriate sensitivity, stability, and biocompatibility, and reliably detected glutamate in neurological monitoring. Implantation experiments in rats demonstrated stable recording and reliable detection of physiological changes in glutamate in response to behavioral/neuronal activation.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Ming Zhao, Yan Cao, I-wen Huang, Harold G. Monbouquette
Summary: Highly sensitive and selective choline microbiosensors were created by using microcontact printing and a crosslinked, polyamine-functionalized zwitterionic polymer matrix. The sensors exhibited higher enzyme loading and spatial resolution, and also showed selectivity against potential interfering compounds.
Article
Chemistry, Analytical
Julija Razumiene, Damiana Leo, Vidute Gureviciene, Dalius Ratautas, Justina Gaidukevic, Ieva Sakinyte-Urbikiene
Summary: Investigations of L-glutamate release can help identify new pathophysiological pathways related to L-glutamate and neurological diseases. A nitrogen-modified graphene oxide biosensor was developed through a one-pot hydrothermal reduction method and used to detect L-glutamate. The biosensor demonstrated stability, ease of preparation, and successful detection of L-glutamate in real samples. Furthermore, it was applied to investigate L-glutamate release in a specific rat strain.
Article
Chemistry, Analytical
Sinem Evli, Deniz Aktas Uygun
Summary: One-dimensional Ni/Au/PPy-COOH nanowires with multiple segments were synthesized and their smooth surfaces and magnetic properties were investigated. These nanowires were used to modify the electrode surface and as a micro-environment for Trametes versicolor laccase. The biosensor based on this design demonstrated high sensitivity, excellent selectivity, wide linear range, and low LOD for the detection of dopamine in biological samples.
Article
Chemistry, Multidisciplinary
Jingyu Xie, Yuchuan Dai, Yu Xing, Yiding Wang, Gucheng Yang, Enhui He, Zhaojie Xu, Penghui Fan, Fan Mo, Yirong Wu, Yilin Song, Xinxia Cai
Summary: Researchers developed a novel method using carboxylated graphene oxide (cGO) to couple glutamate oxidase (GluOx) biomolecules onto neural microelectrodes for sensitive monitoring of glutamate concentration and electrophysiology in epileptic rats. The resulting glutamate IMEA exhibited good performance with less signal crosstalk, lower reaction potential, and higher linear sensitivity.
Article
Neurosciences
Nicola B. Mercuri, Mauro Federici, Francesca Romana Rizzo, Lorenzo Maugeri, Sebastian L. D'Addario, Rossella Ventura, Nicola Berretta
Summary: The activation of metabotropic glutamate receptors leads to a prolonged depression of striatal dopamine release and enhances firing of cholinergic interneurons, resulting in anxiety-like behavior.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2021)
Article
Chemistry, Analytical
Thanih Balbaied, Eric Moore
Summary: The study proposed a method using biosensor-based strategies for real-time monitoring of ALP release from human colon cancer cells, facilitating understanding of cancer progression and emerging diseases. Results showed a correlation between cell response and changes in impedance magnitude and current, influencing cell morphology and viability. The developed method represents an advance over current techniques and may contribute to microfluidic testing development for small-scale techniques.
ANALYTICAL LETTERS
(2022)
Article
Biophysics
Amir Niaraki, Mehran Abbasi Shirsavar, Saurabh S. Aykar, Mehrnoosh Taghavimehr, Reza Montazami, Nicole N. Hashemi
Summary: Real-time and high-throughput monitoring of neural cells exposed to injury mechanisms using microelectrode arrays and electrical impedance spectroscopy is demonstrated in this study. Aqueous graphene is used for microelectrode fabrication, and the surface morphology and electrical properties of the electrodes are characterized. The attachment and impedance changes of the neural population on the graphene microelectrodes are quantified, and the impact of UV exposure on neural detachment is monitored.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Nanoscience & Nanotechnology
Xinshuo Huang, Shantao Zheng, Baoming Liang, Mengyi He, Feifei Wu, Jingbo Yang, Hui-jiuan Chen, Xi Xie
Summary: Monitoring human health is crucial in biomedicine, and blood ion concentrations serve as important reference indicators for many diseases. Microneedle array-based sensors have enabled continuous health monitoring by being minimally invasive. In this study, we developed a microneedle sensing-array integrated system that can continuously detect subcutaneous ions for real-time human health monitoring. The system overcomes limitations in preparing 3D microneedle structures with multiple electrode channels and tackles challenges in modifying closely spaced microneedle tips into different types of electrodes. The system exhibits sufficient sensitivity and good detection performance, as demonstrated by in vivo experiments on rats. By integrating the sensor into a wearable monitoring system, it can potentially provide feedback on diseases related to physiological ion changes.
MICROSYSTEMS & NANOENGINEERING
(2023)
Review
Engineering, Electrical & Electronic
Anneng Yang, Feng Yan
Summary: Flexible electrochemical biosensors are crucial in disease diagnosis and healthcare management, mainly used for detecting biomarkers in various body fluids. These sensors can be employed as wearable devices for in-situ detection and long-term monitoring of target analytes. Transistor-based flexible biosensors exhibit high sensitivity and low detection limits, suitable for multiplexing and high-throughput sensing applications.
ACS APPLIED ELECTRONIC MATERIALS
(2021)
Article
Chemistry, Analytical
Penghui Fan, Yiding Wang, Yuchuan Dai, Luyi Jing, Wei Liang, Botao Lu, Gucheng Yang, Yilin Song, Yirong Wu, Xinxia Cai
Summary: In this study, a fabrication method for a dual-mode high-sensitive flexible micro-electrode array (MEA) probe was proposed, which could simultaneously record single neuron action potentials and dopamine concentrations. The probe was used to detect electrophysiology activities and dopamine in the striatum of awake mice under aversive stimulation. The results showed that fear-induced aversive stimuli led to synchronized decreases in behavior, action potential firing rate, low-frequency local field potential (LFP) power, and dopamine concentration in the striatum.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Sofwan Nuh, Apon Numnuam, Panote Thavarungkul, Tonghathai Phairatana
Summary: This study developed a novel microfluidic-based electrochemical sensor for continuous dopamine detection. By integrating an electrochemical sensor into a microfluidic chip, real-time chemical monitoring with low analyte consumption, short analysis time, low cost, and compact size was achieved. The sensor exhibited high sensitivity, selectivity, and stability, making it a powerful tool for continuous dopamine detection in human clinical applications.
Review
Biochemistry & Molecular Biology
Megana Sundar, Devon Patel, Zachary Young, Kah-Chung Leong
Summary: Recent research suggests that oxytocin may reduce addictive behaviors by directly affecting the glutamatergic system, in addition to its established effects on dopamine. This new perspective provides insight into the potential use of oxytocin as a therapeutic target for addiction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Jin Song, Ashaq Ali, Yaohong Ma, Yiwei Li
Summary: In this study, a microfluidic system using a graphene-modified microelectrode array sensor was proposed for real-time monitoring of bacterial biofilms through electrochemical impedance. The results showed that increasing bacterial cell load on the sensing interface significantly prolonged the observation window period of the biofilm state, thereby improving the sensing signal quality. Simulation studies demonstrated that this method outperformed traditional endpoint detection in anti-biofilm drug screening.
NANOSCALE ADVANCES
(2023)
Article
Biochemical Research Methods
Bonhye Koo, Allison M. Yorita, Jacob J. Schmidt, Harold G. Monbouquette
Article
Neurosciences
Anne L. Collins, Tara J. Aitken, I-Wen Huang, Christine Shieh, Venuz Y. Greenfield, Harold G. Monbouquette, Sean B. Ostlund, Kate M. Wassum
BIOLOGICAL PSYCHIATRY
(2019)
Article
Biophysics
Ximiao Wen, Bo Wang, Shan Huang, Tingyi ''Leo'' Liu, Meng-Shiue Lee, Pei-Shan Chung, Yu Ting Chow, I-Wen Huang, Harold G. Monbouquette, Nigel T. Maidment, Pei-Yu Chiou
BIOSENSORS & BIOELECTRONICS
(2019)
Article
Chemistry, Analytical
Bo Wang, Lili Feng, Bonhye Koo, Harold G. Monbouquette
Article
Biochemistry & Molecular Biology
Mackenzie Clay, Harold G. Monbouquette
Summary: Detailed simulations show that the performance of electroenzymatic glutamate sensors in vitro and in vivo is influenced by mass transfer resistances and clearance rates, leading to unexpected sensor responses in vivo. The distance between the sensor and the location of glutamate release significantly impacts the sensor response, with simulated sensor signals consistently lower than actual glutamate levels for events occurring at distances greater than a few microns. Simulations suggest that detection of glutamate signaling is limited to events within 30 μm of the sensor surface, posing challenges for quantitatively interpreting sensor signal data for glutamate uptake rates from the brain ECS.
ACS CHEMICAL NEUROSCIENCE
(2021)
Review
Biochemical Research Methods
Yan Cao, Zhenrong Zheng, Harold G. Monbouquette
Summary: This review examines a wide range of methods for detecting DNA and RNA at single-digit attomolar concentrations without the need for nucleic acid amplification. These methods correspond to the clinically desirable detection range for pathogen-specific nucleic acid and are close to the detection limits of commercially available nucleic acid amplification tests based on PCR. With the ongoing pandemic, the demand for faster, more cost-effective, yet highly accurate tests has become increasingly evident.
CURRENT OPINION IN BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Chuanzhen Zhao, Kevin M. Cheung, I-Wen Huang, Hongyan Yang, Nako Nakatsuka, Wenfei Liu, Yan Cao, Tianxing Man, Paul S. Weiss, Harold G. Monbouquette, Anne M. Andrews
Summary: Implantable aptamer-field-effect transistor (FET) neuroprobes were developed for monitoring neurotransmitters, enabling femtomolar serotonin detection limits in brain tissue. This study opens opportunities for integrated neural activity recordings at high spatiotemporal resolution by combining these aptamer-FET sensors with other types of Si-based implantable probes.
Article
Chemistry, Analytical
I-wen Huang, Mackenzie Clay, Yan Cao, Jingjing Nie, Yuwan Guo, Harold G. Monbouquette
Summary: By optimizing the thickness of the immobilized ChOx layer and minimizing permselective polymer coatings, the performance of choline sensors can be significantly improved. Electroenzymatic choline sensors with a 5μm thick ChOx layer and 200 nm thick permselective polymer coatings exhibited unprecedented sensitivity and response time.
Article
Chemistry, Analytical
I-wen Huang, Mackenzie Clay, Siqi Wang, Yuwan Guo, Jingjing Nie, Harold G. Monbouquette
Meeting Abstract
Chemistry, Multidisciplinary
Chuanzhen Zhao, I-Wen Huang, Kevin Cheung, Nako Nakatsuka, Hongyan Yang, Leonardo Scarabelli, Liv Heidenreich, Jason Belling, Paul Weiss, Harold Monbouquette, Anne Andrews
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Meeting Abstract
Chemistry, Multidisciplinary
I-Wen Huang, Mackenzie Clay, Harold Monbouquette
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Meeting Abstract
Chemistry, Multidisciplinary
I-Wen Huang, Mackenzie Clay, Harold Monbouquette
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)
Meeting Abstract
Chemistry, Multidisciplinary
Chuanzhen Zhao, I-Wen Huang, Nako Nakatsuka, Paul Weiss, Harold Monbouquette, Anne Andrews
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Biochemistry & Molecular Biology
Mackenzie Clay, Harold G. Monbouquette
ACS CHEMICAL NEUROSCIENCE
(2018)
Article
Biochemical Research Methods
Bo Wang, Ximiao Wen, Yan Cao, Shan Huang, Hoa A. Lam, Tingyi Leo Liu, Pei-Shan Chung, Harold G. Monbouquette, Pei-Yu Chiou, Nigel T. Maidment
