Article
Agronomy
Jian Li, Pengfei He, Pengbo He, Yongmei Li, Yixin Wu, Zhanjun Lu, Xingyu Li, Yongchao Yang, Yuehu Wang, Jun Guo, Shahzad Munir, Yueqiu He
Summary: The study found that Bacillus subtilis L1-21 can effectively reduce the quantity of CLas pathogen in ACP, while easily colonizing the midgut of ACP. The highest colonization of endophyte L1-21 was observed in ACP after feeding on new citrus leaves. Psyllids carrying endophyte L1-21 after feeding on citrus leaves were able to reduce the CLas pathogen quantity in citrus leaves.
PEST MANAGEMENT SCIENCE
(2022)
Article
Plant Sciences
Yang Hu, Youqing Meng, Liangjin Yao, Enguo Wang, Tao Tang, Yunsheng Wang, Liangying Dai, Mingping Zhao, Hong-en Zhang, Xiaoyan Fan, Luyun Luo, Wei Xiang, Zhuo Zhang
Summary: This study systematically analyzed the factors related to citrus Huanglongbing and found that the number of bacteria carried by Diaphorina citri and the changes in citrus phyllosphere microbiome are correlated with the incidence of Huanglongbing.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Entomology
Erik L. Roldan, Lukasz L. Stelinski, Kirsten S. Pelz-Stelinski
Summary: The use of oxytetracycline and streptomycin in citrus trees can reduce the acquisition of Candidatus Liberibacter asiaticus (CLas) bacteria by the Asian citrus psyllid, but it does not prevent or reduce tree infection. The use of mesh screening can prevent bacterial infection.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Plant Sciences
Emily M. T. Padhi, Karla J. Araujo, Elizabeth Mitrovic, Marylou Polek, Kris E. Godfrey, Carolyn M. Slupsky
Summary: This study used a metabolomics approach to investigate the changes in leaf metabolites during the early stages of Huanglongbing (HLB). The results showed that infection with Candidatus Liberibacter asiaticus caused significant alterations in leaf metabolome, enabling discrimination between healthy and infected plants.
Article
Plant Sciences
Yanjing Wang, Takumasa Kondo, Yurong He, Zhuohuai Zhou, Jinming Lu
Summary: The study presents the genome sequence of CLas strain CoFLP1 collected from Colombia, contributing to the understanding of the introduction pathway of HLB in South America. This first CLas genome sequence from Colombia will enhance CLas genome resources.
Article
Virology
Longtong Chen, Yangyang Liu, Fengnian Wu, Jingtian Zhang, Xiaoqing Cui, Shitong Wu, Xiaoling Deng, Meirong Xu
Summary: This study investigates the acquisition and transmission mechanisms of Huanglongbing (HLB) pathogen and Citrus tristeza virus (CTV) in Diaphorina citri (Asian citrus psyllid). The results suggest that the HLB pathogen may inhibit the acquisition of CTV by the psyllid. Interestingly, the presence of CTV in the psyllid facilitates the acquisition and transmission of HLB pathogen.
Article
Plant Sciences
Xu Wei, Amany Mira, Qibin Yu, Fred G. Gmitter
Summary: RNA-seq analysis was used to study the early transcriptional changes in citrus responding to Candidatus Liberibacter asiaticus (CaLas) transmitted by the Asian citrus psyllid. The results showed delayed defense reaction in Valencia sweet orange (VAL) against infective vector, while immediate and substantial transcriptomic changes were observed when attacked by healthy vector. Moreover, infection by CaLas-infected psyllids led to significant gene expression changes in important pathways at different time points.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Microbiology
Marcelo L. Merli, Kaylie A. Padgett-Pagliai, Alexandra E. Cuaycal, Lucila Garcia, Maria Rosa Marano, Graciela L. Lorca, Claudio F. Gonzalez
Summary: The study revealed that a potential effector LotP of 'Candidatus Liberibacter asiaticus' may interact with chaperones like GroEL and Hsp40 to enhance Lon protease activity, leading to damage in citrus plant tissues. LotP may also interact with plant proteins, indicating its potential role in protease activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaoge Nian, Yaru Luo, Xinyu He, Shujie Wu, Jiayun Li, Desen Wang, Paul Holford, George Andrew Charles Beattie, Yijing Cen, Songdou Zhang, Yurong He
Summary: The study found that the pathogen 'Candidatus Liberibacter asiaticus' (CLas) promotes ovarian development and increased egg production in Diaphorina citri. The miRNA miR-275 was identified as a key factor in regulating egg production by suppressing the expression of the vitellogenin receptor (DcVgR). Additionally, the Juvenile Hormone (JH) pathway was found to play a role in shaping the observed fecundity phenotype. These findings suggest that CLas manipulates the JH signaling pathway and miR-275 to increase the reproductive capacity of D. citri, potentially resulting in a mutualistic relationship between the pathogen and vector host.
Article
Microbiology
Yasser E. Ibrahim, Jorge R. Paredes-Montero, Mohammed A. Al-Saleh, Arya Widyawan, Ruifeng He, Mahmoud H. El Komy, Hathal M. Al Dhafer, Noel Kitchen, David R. Gang, Judith K. Brown
Summary: This study investigated the citrus greening disease in Saudi Arabia and found that it is caused by the Asian citrus psyllid transmitting the Candidatus Liberibacter asiaticus. The study also analyzed the genetic makeup of the psyllids and identified two major lineages of the pathogen.
Article
Multidisciplinary Sciences
Qi Pan, Shi-Jiang Yu, Shuang Lei, Si-Chen Li, Li-Li Ding, Liu Liu, Lu-Yan Cheng, Ren Luo, Cui-Yun Lei, Bing-Hai Lou, Lin Cong, Hao-Qiang Liu, Xue-Feng Wang, Chun Ran
Summary: Asian citrus psyllid is a significant vector of Huanglongbing, the most devastating citrus disease worldwide. The study shows that Huanglongbing can affect the abundance of gut endosymbionts in the Asian citrus psyllid.
Article
Entomology
Shuang Lei, Shi-Jiang Yu, Qi Pan, Li-Li Ding, Si-Chen Li, Lu-Yan Cheng, Shu-Qi Wang, Bing-Hai Lou, Jun He, Cui-Yun Lei, Lin Cong, Hao-Qiang Liu, Xue-Feng Wang, Chun Ran
Summary: In this study, a high-quality chromosome-level genome of Diaphorina citri, a global citrus pest, was successfully generated using DNBSEQ(TM), Oxford Nanopore Technologies, and Hi-C technologies. The genome size was determined to be 523.78 Mb and 24,048 protein-coding genes were predicted. The study also revealed the XO type sex chromosome system of D. citri and its closest relative, Pachypsylla venusta. The findings provide valuable information for the effective management of D. citri.
Article
Microbiology
Rui-Xu Jiang, Feng Shang, Hong-Bo Jiang, Wei Dou, Tomislav Cernava, Jin-Jun Wang
Summary: Environmental factors can influence the bacterial community structure and relative abundance in ACP populations, which is important for better management of HLB transmission.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Tao Peng, Yingzhe Yuan, Aijun Huang, Jun He, Shimin Fu, Shuo Duan, Long Yi, Chenyang Yuan, Huizhu Yuan, Xuefeng Wang, Changyong Zhou
Summary: Huanglongbing (HLB) is a devastating citrus disease caused by Candidatus Liberibacter asiaticus (CLas) and mainly transmitted by Asian citrus psyllid (ACP, Diaphorina citri). The protein-protein interactions between CLas and D. citri are still unknown.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Bernard Reynaud, Patrick Turpin, Florencia M. Molinari, Martial Grondin, Solene Roque, Frederic Chiroleu, Alberto Fereres, Helene Delatte
Summary: Huanglongbing (HLB) is a serious disease of citrus worldwide, caused by three non-cultivable bacteria. The African psyllid Trioza erytreae is capable of efficiently acquiring and transmitting the bacteria, highlighting the high risk of spread of the disease in Europe.
FRONTIERS IN PLANT SCIENCE
(2022)