Article
Biochemistry & Molecular Biology
Martin Panigaj, Michael P. Marino, Jakob Reiser
Summary: LV vectors have faced challenges in in vivo gene therapy applications, but engineering approaches can improve their specificity and stability for more effective gene delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Virology
Valentina Poletti, Fulvio Mavilio
Summary: Lentiviral vectors are commonly used in gene therapy for monogenic diseases, where they transduce autologous somatic cells in an ex vivo setting for re-delivery to patients. Key applications include hematopoietic stem cells for hematological or neurometabolic diseases and T cells for cancer immunotherapy.
Article
Virology
Nicole Cordes, Nora Winter, Carolin Kolbe, Bettina Kotter, Joerg Mittelstaet, Mario Assenmacher, Toni Cathomen, Andrew Kaiser, Thomas Schaser
Summary: This study reports the development of a versatile targeting system for MV-LVs, which enables selective gene transfer by mixing with biotinylated adapter molecules. The system demonstrates high efficiency and flexibility, making it suitable for improving the safety and efficacy of cellular therapies.
Article
Genetics & Heredity
Kuba Nowak, Pawel Blazej, Malgorzata Wnetrzak, Dorota Mackiewicz, Pawel Mackiewicz
Summary: Reprogramming the standard genetic code to incorporate non-canonical amino acids offers new possibilities for medicine, industry, and biotechnology. Various methods of code engineering are available to store new genetic information and produce proteins with novel properties. The study provides a theoretical background for optimal genetic code expansion, discussing the robustness of the new coding system and the analysis of optimal codon sets using graph theory.
Review
Microbiology
Kexin Meng, Christina Z. Chung, Dieter Soell, Natalie Krahn
Summary: This review discusses the diverse genetic code systems of archaea, detailing the associated biochemical elements and molecular mechanisms. Archaea possess unique genetic code systems that can encode rare amino acids and utilize alternative pathways to synthesize and incorporate canonical amino acids.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Khomkrit Suppakhaw, Krittapas Jantarug, Sarah Slavoff, Nipan Israsena, Chayasith Uttamapinant
Summary: A new labeling strategy has been developed in this study for proteolytic proteoforms, showcasing its utility in detecting amyloid-beta peptides.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Biochemical Research Methods
Jessica T. Stieglitz, Kelly A. Potts, James A. Van Deventer
Summary: The study introduces single plasmid systems (SPSs) for genetic code expansion in yeast and compares their noncanonical amino acid (ncAA) incorporation capabilities with previously described dual plasmid systems (DPSs). Results show that SPSs perform equally well as DPSs in terms of ncAA incorporation efficiency, making them a convenient alternative. This suggests that SPSs can be used for high-throughput investigations on genetic or genomic changes affecting ncAA incorporation efficiency and the eukaryotic translation apparatus.
ACS SYNTHETIC BIOLOGY
(2021)
Editorial Material
Chemistry, Multidisciplinary
Mirren F. M. White, Stephen Wallace
Summary: The discovery and engineering of new plastic degrading enzymes is crucial for the transition to a more sustainable and circular plastics economy. Recent research has reported the discovery of a novel PET hydrolase from human saliva metagenome, which shows improved properties and performance over previous PETases. The study also demonstrates the incorporation of a photocaged unnatural amino acid, enabling covalent binding to the PET surface, highlighting the potential of metagenomic datasets and genetic code expansion for new biotechnologies in the circular plastics economy.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Multidisciplinary Sciences
Tiberiu S. Mihaila, Carina Bate, Lynn M. Ostersehlt, Jasmin K. Pape, Jan Keller-Findeisen, Steffen J. Sahl, Stefan W. Hell
Summary: With the recent advancements in fluorescence nanoscopes, the ability to dissect nanoscopic biological structures is limited by the size of protein labels. Bioorthogonal chemical groups provide a powerful tool for specific detection of biomolecules. In this study, we introduced an engineered aminoacyl-tRNA synthetase/tRNA pair to allow for the site-specific introduction of amino acids with clickable side chains into proteins. This enhanced genetic code expansion machinery was then used to label beta-actin in cells for high-resolution imaging. Our findings highlight the need for further improvements in labeling methods to fully exploit the potential of molecular-scale optical three-dimensional resolution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Bhumrapee Eiamthong, Piyachat Meesawat, Thanakrit Wongsatit, Jariya Jitdee, Raweewan Sangsri, Maturada Patchsung, Kanokpol Aphicho, Surased Suraritdechachai, Nicolas Huguenin-Dezot, Shan Tang, Wipa Suginta, Boonchoat Paosawatyanyong, M. Madan Babu, Jason W. Chin, Danaya Pakotiprapha, Worawan Bhanthumnavin, Chayasith Uttamapinant
Summary: We report the discovery of a new PET hydrolase, named MG8, from the human saliva metagenome. MG8 exhibits robust PET degradation activities under different conditions and outperforms other hydrolases in degrading PET. Furthermore, we genetically modified MG8 to serve as a covalent binder for PET functionalization, enabling the attachment of protein cargos to PET and other polyester plastics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Xue-Yuan Wu, Meng-Yin Li, Shao-Jun Yang, Jie Jiang, Yi-Lun Ying, Peng R. Chen, Yi-Tao Long
Summary: Conventional methods for protein nanopore engineering are limited by the 20 natural amino acids, which restricts the diversity of nanopores. To overcome this, we used the genetic code expansion technique to incorporate unnatural amino acids into aerolysin nanopores. Molecular dynamics simulations and single-molecule sensing experiments showed that the newly incorporated amino acids provided a favorable environment for target molecule interactions. This work provides a new framework for creating nanopores with unique sensing properties.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biology
Erika Alden DeBenedictis, Dieter Soll, Kevin M. Esvelt
Summary: This study investigates the tolerance of the Escherichia coli genetic code to tRNA mutations and found that while most tRNAs can be converted to a quadruplet form, only nine aminoacyl tRNA synthetases can tolerate quadruplet anticodons. This limits the chemical alphabet available for an all-quadruplet code.
Article
Chemistry, Multidisciplinary
Jing Yang, Kefan Wang, Shanyu Zhang, Xiaocui Zheng, Tongxiao Cui, Xian Yang, Yao Liu, Di Lu, Yuqin Wang, Xiao Tian, Panke Zhang, Shuo Huang, Ran Xie
Summary: This study presents a genetic code expansion strategy to introduce unnatural amino acids into the Mycobacterium smegmatis porin A (MspA) nanopore, enabling the rapid and efficient introduction of bioorthogonal reactive sites and the conjugation with single stranded DNA or lysozyme. The lysozyme-conjugated pore demonstrated the ability to discriminate between different oligosaccharides, which a bare MspA nanopore does not possess. This genetic code expansion strategy with bioorthogonal handles is a versatile method for nanopore engineering and may expand the application scenarios of nanopores.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Pharmacology & Pharmacy
Xintong Zhang, Yanhong Liu, Congcong Xiao, Youyan Guan, Zhonggao Gao, Wei Huang
Summary: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that affects approximately 1% of the global population. Gene therapy may hold potential as a treatment option for RA, as many existing therapeutic drugs have severe side effects. The use of nanoparticle delivery systems can improve the stability of nucleic acids and enhance transfection efficiency in vivo, thereby making gene therapy for RA safer and more effective. This review provides an overview of current nanomaterials and targeting ligands used in RA gene therapy, as well as various gene delivery systems that may inform future research.
Editorial Material
Microbiology
Leo C. James
Summary: Researchers have developed a direct labeling technology using genetic code expansion and click chemistry to label HIV-1 capsids with a single modified amino acid. They demonstrated that the capsids remain intact during their transportation into the nucleus of T cells, supporting a late model of uncoating. This new technology has the potential to greatly enhance future studies by enabling the combination of labeled capsids with fluorescent proteins or host factors.
Article
Engineering, Mechanical
Jiawei Ji, Xiaojun Liu, Yan Zhang, Yunlong Jiao, Kun Liu
Summary: This study examines the effect of surface connectivity and three-dimensional topography parameters on the friction characteristics of a sliding contact interface. The results show that increasing surface connectivity improves lubricant storage and flow, leading to a decrease in the friction coefficient. Additionally, the variation of functional morphology parameters also affects the friction property.
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART J-JOURNAL OF ENGINEERING TRIBOLOGY
(2023)
Review
Oncology
Xing Yan, Changhong Liu
Summary: Lung cancer is a rapidly increasing malignant cancer in China, and surgical treatment is the only effective treatment. However, postoperative local recurrence rate is high, mainly due to minimal residual disease. Monitoring circulating tumor DNA can assess the level of minimal residual disease and guide personalized postoperative treatment.
Article
Engineering, Multidisciplinary
Jie Wang, Xi Lu, Pengfei Du, Haotian Zheng, Zhaoxin Dong, Zihua Yin, Jia Xing, Shuxiao Wang, Jiming Hao
Summary: A new synergistic indicator is introduced to evaluate the synergistic effects of carbon mitigation on air pollutant reduction and public health in China. The results show that synergistic effects played an increasingly important role in emissions mitigation, leading to reductions in pollutants and improvements in air quality and public health. With urbanization and carbon neutrality, synergistic effects are expected to continue to increase.
Article
Metallurgy & Metallurgical Engineering
Tao Zhang, Yue Chen, Guoguang Cheng, Xinlong Yang, Xingrun Chen, Jixiang Pan, Yu Huang, Qiming Wang
Summary: Industrial experiments were conducted to investigate the precipitation and growth of AlN in Fe-23Mn-2Al-0.08 V steel with high nitrogen content. The morphology of AlN includes hexagonal and needle-like shapes. The size of AlN increases and the total amount decreases as the cooling rate decreases. Aluminum concentration is low in interdendritic regions but high in dendrites, with almost no nitrogen segregation. Thermodynamic calculation and kinetic analysis method reveal the growth trend of AlN.
STEEL RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Physical
Qinglei Wu, Chenyang Shen, Chang-jun Liu
Summary: The use of an amino acid modified Pd/SiO2 catalyst suppresses the excessive hydrogenation capacity of palladium, leading to improved selectivity for hydrogenation of acetylene.
APPLIED SURFACE SCIENCE
(2023)
Article
Fisheries
Moamer A. H. Ahmed, Jiabao Ma, Shuai Shao, Qiyao Wang, Rongjing Xu, Kaiyu Yin
Summary: In this study, it was discovered that a PDZ-domain-containing protease (Prc) regulated the expression of esrB and T3/T6SSs in different manners at different growth stages. Additionally, Prc mediated the regulation of environmental acidic signals on the expression of esrB. Mutation of esrA impaired E. piscicida virulence expression irrespective of the ambient acidic signals. These findings provide a comprehensive understanding of the virulence regulation upon sensing the environmental acidic signals in bacterial pathogens.
Article
Engineering, Electrical & Electronic
Liqi Yan, Qifan Wang, Siqi Ma, Jingang Wang, Changbin Yu
Summary: This paper presents a weakly supervised framework called STC-Seg for instance segmentation and tracking in videos, which utilizes spatio-temporal collaboration. It generates effective pseudo-labels for training deep networks and predicting high-quality instance masks by leveraging the complementary representations from unsupervised depth estimation and optical flow. The framework also enhances mask generation with a puzzle loss and improves robustness to different object appearances through a tracking module that jointly utilizes bounding-box diagonal points with spatio-temporal discrepancy.
IEEE TRANSACTIONS ON CIRCUITS AND SYSTEMS FOR VIDEO TECHNOLOGY
(2023)
Article
Chemistry, Physical
Nutthaphak Kitiphatpiboon, Meng Chen, Changrui Feng, Yifan Zhou, Changlin Liu, Zhongbao Feng, Qiang Zhao, Abuliti Abudula, Guoqing Guan
Summary: Currently, electrolysis of seawater-based electrolytes for hydrogen production is gaining attention. A composite electrocatalyst, MnCo2O4@NiFe-LDH, was developed for seawater electrolysis, which exhibited low overpotentials and improved stability. The NiFe-LDH layer acted as a protective layer and improved the active surface area of MnCo2O4 nanowires. Additionally, high valence states of Mn3+, Co3+, Ni3+, and Fe3+ played a vital role in the oxygen evolution reaction. This composite electrocatalyst shows promise for long-term seawater splitting.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Materials Science, Multidisciplinary
Xinxiu Wang, Ruirun Chen, Qi Wang, Shu Wang, Yili Li, Yanqing Su, Yuan Xia, Guoping Zhou, Guanglong Li, Yingdong Qu
Summary: Reasonable selection of process parameters can affect the temperature and flow fields of metal liquid in the mold cavity and achieve the desired casting organization. This study simulated the temperature and flow fields of high molybdenum cast iron casting under different casting temperatures and mold preheating temperatures. The results indicate that casting temperature has a strong impact on the movement state of the metal liquid, while mold preheating temperature influences the solidification rate and cooling time of the casting.
JOURNAL OF MATERIALS ENGINEERING AND PERFORMANCE
(2023)
Article
Engineering, Marine
Yi Ren, Yuxuan Qin, Fuzhen Pang, Hongfu Wang, Yumin Su, Haichao Li
Summary: The flow-induced structure noise of underwater vehicles is mainly caused by the structural vibration under turbulent fluctuating pressure. The complex coupling relationship and energy transfer among flow, structure, and sound are involved. In this paper, a hybrid numerical method based on computational fluid dynamics (CFD) and computational acoustics (CA) is used to simulate the flow-induced structure noise of a submerged cone-cylinder-hemisphere combined shell. An experiment is conducted in a low-noise gravity water tunnel to validate the efficiency and accuracy of the numerical method. The results show that the energy of flow-induced structure noise is concentrated in the medium and low frequency range. The line-spectrum characteristics of radiated noise are consistent with the inherent vibration characteristics of the structure.
Article
Materials Science, Multidisciplinary
Yang Liu, Yuliang Wu, Tong Wang, Qian Wang, Xianchao Han, Xiaofu Wu, Hui Tong, Lixiang Wang
Summary: A series of host materials containing a hole-transport triphenyl-substituted triazatruxene conjugated skeleton and different numbers of diphenyl methylene-bridging groups were developed for solution-processed yellow TADF OLEDs. These host materials exhibited high triplet energy, effectively separated the TADF emitters, and showed high thermal stability and good solubility. The OLED using 2FTAT-1Ph as host achieved low turn on voltage, high external quantum efficiency, and remarkable power efficiency, demonstrating the potential of diphenyl methylene bridged tri-azatruxene derivatives as high-performance host materials for solution-processed TADF OLEDs.
ORGANIC ELECTRONICS
(2023)
Article
Microbiology
Haiping Ni, Yuxia Wu, Rui Zong, Shiai Ren, Deng Pan, Lei Yu, Jianwei Li, Zhuling Qu, Qiyao Wang, Gengxing Zhao, Jianzhong Zhao, Lumin Liu, Tao Li, Youming Zhang, Qiang Tu
Summary: Soil salinization and acidification seriously damage soil health and restrict the sustainable development of planting. This study investigated the effect of phosphate-solubilizing bacteria and nitrogen-fixing bacteria on crop quality, soil physicochemical properties, and microbial communities in acid and salinized soil. The results showed that the inoculated strains significantly influenced the soil properties and crop quality of cucumber and lettuce. The combination of the two bacteria also affected the diversity and richness of bacterial community in the lettuce-grown soil.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Plant Sciences
Qi Wu, Xue Bai, Mengping Nie, Li Li, Yiming Luo, Yu Fan, Changying Liu, Xueling Ye, Liang Zou
Summary: This study identified 23 PEBP genes in quinoa and determined their roles in growth and flowering. Transcription factors that may regulate flowering in quinoa were also discovered. These findings are significant for future quinoa breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Physical
Cuicui Wang, Yuhao Zhang, Yifan Liu, Qian Chen, Yingxuan Song, Anqi Cai, Hongwei Guo, Pei Zhang
Summary: Pure and alkaline earth metal elements (Mg2+, Ca2+ and Sr2+) doped NaTaO3 nanofilms were prepared by a simple hydrothermal method, showing better stability and recycling performance in wastewater treatment. The band gaps of NaTaO3 were reduced to 2.83, 3.01 and 2.96 eV after doping with Mg2+, Ca2+ and Sr2+. Mg-NaTaO3 catalyst exhibited a higher photodegradation efficiency of 80.1% for methylene blue solution under visible light irradiation and a strong inhibition effect on E. coli and S. aureus.
APPLIED SURFACE SCIENCE
(2023)
Article
Environmental Sciences
Hua-Jie Wang, Meng-Fei Hao, Guan Wang, Hao Peng, Fazli Wahid, Yan Yang, Lei Liang, Shan-Qin Liu, Ren-Long Li, Shu-Ying Feng
Summary: A combination of 3D printing and coating technique was used to prepare functional polyurethane stents for the prevention of thrombosis and infection. The functional stents demonstrated suitable mechanical properties and biological compatibility, as well as anti-coagulation and antibacterial effects.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
