Journal
NUCLEIC ACIDS RESEARCH
Volume 43, Issue 14, Pages 7058-7069Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkv643
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Funding
- Medical Research Council [U105178806]
- Leukaemia & Lymphoma Research [11018, 06011, 10017]
- Netherlands Organisation for Scientific Research (NWO) [825.09.003]
- MRC [MC_U105178806] Funding Source: UKRI
- Medical Research Council [MC_U105178806] Funding Source: researchfish
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In order to catalyse the splicing of messenger RNA, multiple proteins and RNA components associate and dissociate in a dynamic highly choreographed process. The Prp19 complex is a conserved essential part of the splicing machinery thought to facilitate the conformational changes the spliceosome undergoes during catalysis. Dynamic protein interactions often involve highly disordered regions that are difficult to study by structural methods. Using amine cross-linking and hydrogen-deuterium exchange coupled to mass spectrometry, we describe the architecture of the Prp19 sub-complex that contains CTNNBL1. Deficiency in CTNNBL1 leads to delayed initiation of cell division and embryonic lethality. Here we show that in vitro CTNNBL1 enhances the association of CWC15 and CDC5L, both core Prp19 complex proteins and identify an overlap in the region of CDC5L that binds either CTNNBL1 or CWC15 suggesting the two proteins might exchange places in the complex. Furthermore, in vivo, CTNNBL1 is required to maintain normal levels of the Prp19 complex and to facilitate the interaction of CWC15 with CDC5L. Our results identify a chaperone function for CTNNBL1 within the essential Prp19 complex, a function required to maintain the integrity of the complex and to support efficient splicing.
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