Journal
JOURNAL OF DENTISTRY
Volume 40, Issue -, Pages E26-E34Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.jdent.2012.02.010
Keywords
Hydrogen peroxide; Enamel; Tooth bleaching; ATR-IR; Raman; Color; In situ; In vitro
Categories
Funding
- Natural Science Foundation of China [81071190]
- Youth Chenguang Project of Science and Technology of Wuhan City [200950431186]
- Fundamental Research Funds for the Central Universities [41030030]
- Self-Research Programme for Doctoral Candidates of Wuhan University
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Objectives: To investigate in situ and in vitro effects of acidic and neutral in-office bleaching agents on human enamel in terms of chemical structure and tooth color. Methods: Ninety enamel slabs were obtained from premolars. Then specimens were randomly distributed into six groups (n = 15) and the human saliva (HS) in volunteers' oral cavities was used to simulate in situ condition: group Beyond + distilled water (DW); group Opalescence Boost (O-Boost) + DW; group control + DW; group Beyond + HS; group O-Boost + HS and group control + HS. Twice in-office bleaching treatments were performed with a one-week interval and the total bleaching time was 90 min. ATR-IR spectroscopy, Raman spectroscopy and color measurement were performed before the bleaching treatment and after one week post-treatment, respectively. Then ATR-IR and Raman spectroscopies were analysed and the carbonate: mineral (C:M) ratio, Raman absolute intensity (RAI), Raman relative intensity (RRI) and laser-induced fluorescence intensity (FI) were calculated for evaluation. Results: C:M ratio and percentage RRI showed significantly decrease in group Beyond + DW (p < 0.001, p < 0.001) while little variation was observed in the other groups (p > 0.05, p > 0.05). Percentage FI and Delta E revealed statistical difference in all bleached groups (p < 0.001, p < 0.001) while no statistical difference in control groups (p > 0.05, p > 0.05). Conclusion: Acidic and neutral in-office bleaching agents had the same whitening efficiency in situ and in vitro. Acidic agents could induce demineralisation of human enamel in vitro and the presence of natural human saliva could minimise this adverse effect. (C) 2012 Elsevier Ltd. All rights reserved.
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