Article
Biophysics
Zhiwei Jiang, Jin He, Xueting Wang, Danji Zhu, Na Li, Lingfei Ren, Guoli Yang
Summary: Nanomaterial-based cell sheet technology has significant potential in regenerative medicine and tissue engineering. Different types of nanomaterials have been used to harvest cell sheets, categorized by mechanisms such as light-induced, thermo-responsive, magnetic-controlled, and ROS-induced. Studies have demonstrated successful outcomes in the regeneration of bone, skeletal muscle, cardiac tissue, tendon, angiogenesis, and osseointegration. However, challenges remain in terms of comprehensive preparation, safety, and cell quality.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Review
Biochemistry & Molecular Biology
Maria Grazia Tupone, Gloria Panella, Michele d'Angelo, Vanessa Castelli, Giulia Caioni, Mariano Catanesi, Elisabetta Benedetti, Annamaria Cimini
Summary: Nanomaterials, particularly graphene-based ones, exhibit attractive biological and chemical characteristics due to their small size and interaction with cells and tissues, offering potential applications in biomedicine. Graphene and its derivatives have been extensively used in various biomedical research areas, showing excellent properties for tissue engineering and drug delivery. The use of graphene-based nanomaterials, including combined with other smart materials, holds promise in nervous tissue engineering and neural regenerative medicine, with a focus on stimulating neural growth and regeneration for clinical applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Agnese Gugliandolo, Luigia Fonticoli, Oriana Trubiani, Thangavelu S. Rajan, Guya D. Marconi, Placido Bramanti, Emanuela Mazzon, Jacopo Pizzicannella, Francesca Diomede
Summary: Tissue engineering has become a major focus of research, with the potential of biomaterials and mesenchymal stem cells (MSCs) showing promise for bone regeneration, particularly in oral bone regeneration. Combining MSCs with biomaterials enhances bone regenerative power, and utilizing the MSC secretome also presents a promising future for bone tissue regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Polymer Science
Weixing Chen, Yue Ming, Min Wang, Mingshu Huang, Hongyu Liu, Yisheng Huang, Zhijie Huang, Ling Qing, Qin Wang, Bo Jia
Summary: Regenerative medicine aims to transform clinical medicine through curative strategies, and the development of this field relies on multifunctional biomaterials. Hydrogels, which resemble the natural extracellular matrix and have good biocompatibility, are of interest in bioengineering and medical research. However, traditional hydrogels lack sufficient internal structure and cross-linking modes to achieve desired functions and stability. Introducing multifunctional nanomaterials into 3D hydrogel networks addresses these limitations and enables the display of various functionalities.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Review
Biotechnology & Applied Microbiology
Mireille M. J. P. E. Sthijns, Clemens A. van Blitterswijk, Vanessa L. S. LaPointe
Summary: This review proposed a novel strategy to generate tissue-specific ECM by releasing metabolic molecules through a synthetic material, which stimulates cells to form their own ECM. The approach is based on the modulation of ECM properties via cellular metabolism, targeting different levels of metabolism to achieve desired effects.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Olga Krasilnikova, Anna Yakimova, Sergey Ivanov, Dmitri Atiakshin, Andrey A. Kostin, Dmitry Sosin, Peter Shegay, Andrey D. Kaprin, Ilya Klabukov
Summary: Gene-activated materials (GAMs) offer a promising approach for the treatment of defects in the oral and maxillofacial regions by combining tissue engineering and gene therapy. GAMs involve implanting biocompatible materials enriched with gene-carrying vectors into the defect site to transfect recipient cells and secrete therapeutic proteins. Various designs of GAMs can be used depending on the material, encoded protein, vector, and connection method. Plasmid-based GAMs have shown particular interest in the treatment of periodontitis, jaw bone defects, teeth avulsion, and other pathologies in the oral and maxillofacial regions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Priti Gupta, Shilpa Sharma, Shagufta Jabin, Sapana Jadoun
Summary: Regenerative medicine and tissue engineering have great potential in addressing the limitations of traditional medical approaches. Chitosan, as a naturally derived biocompatible biomaterial, has played a significant role in tissue regeneration. It has unique properties and can be applied in various fields, such as agriculture, nutraceuticals, biomedicine, and food. Chitosan is also an important material for developing next-generation hydrogels and bio-scaffolds. Furthermore, chitosan can be easily modified to enhance its mechanical strength, biodegradability, and controlled release of bioactive molecules. Blending chitosan with other polymers or incorporating nanoparticles expands its possibilities in tissue engineering applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Engineering, Biomedical
Qi Feng, Dingguo Li, Qingtao Li, Xiaodong Cao, Hua Dong
Summary: This mini-review summarizes the fabrication strategies, unique characteristics, and applications of microgel assembly in tissue engineering and regenerative medicine, with a focus on 3D printing. The challenges for further applications of microgel assembly are also discussed.
BIOACTIVE MATERIALS
(2022)
Review
Biotechnology & Applied Microbiology
Zhanqi Wei, Ganlin Zhang, Qing Cao, Tianhao Zhao, Yixin Bian, Wei Zhu, Xisheng Weng
Summary: Nanomaterials, especially organic nanocomposites, hold great promise for the repair and regeneration of cartilage tissue due to their ability to mimic the microstructure and properties of natural cartilage, enhancing scaffold-cell interactions and improving the functionality of engineered tissue constructs.
BIOENGINEERING-BASEL
(2022)
Review
Pharmacology & Pharmacy
Izeia Lukin, Itsasne Erezuma, Lidia Maeso, Jon Zarate, Martin Federico Desimone, Taleb H. Al-Tel, Alireza Dolatshahi-Pirouz, Gorka Orive
Summary: Tissue engineering has become a viable medical alternative in response to the increasing lifespan of society. This review focuses on gelatin, a widely used biomaterial in tissue engineering, and discusses ongoing concerns and the latest developments in this field. Advancements in technology, such as 3D bioprinting and gelatin functionalization, have allowed for better replication of native tissue in gelatin-based systems. This breakthrough has implications for both the manufacturing process, making it more practical and cost-effective, and the future use of gelatin in disease detection and diagnosis rather than treatment.
Review
Biochemistry & Molecular Biology
Nikita Voloshin, Pyotr Tyurin-Kuzmin, Maxim Karagyaur, Zhanna Akopyan, Konstantin Kulebyakin
Summary: Immortalized cells are increasingly used in practical medicine due to their advantages, such as the ability to produce larger amounts of cells and use them for longer periods of time. However, they also have drawbacks, like the possibility of malignant transformation or major phenotype change. This review discusses ways to overcome these barriers and highlights specific fields of application where these concerns are minimal.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Multidisciplinary
Sarah M. Hull, Lucia G. Brunel, Sarah C. Heilshorn
Summary: Using gel-phase materials as bioinks offers advantages in providing cell protection and biological signals. Challenges remain in terms of cell compatibility and biological functionality of bioink materials.
ADVANCED MATERIALS
(2022)
Review
Dentistry, Oral Surgery & Medicine
Lamia Singer, Ahmed Fouda, Christoph Bourauel
Summary: Biomimetics is a field that explores the technical beauty of nature and has been successfully applied in various applications, such as the design of the Eiffel Tower. In dentistry, biomimetic concepts and protocols are used to preserve tooth structure and increase the longevity of restorative dental treatments. Biomimetic dental materials have excellent physico-chemical properties and have been proven to overcome limitations of earlier available materials.
Review
Chemistry, Multidisciplinary
Lin Bao, Xuejing Cui, Monika Mortimer, Xiaoyu Wang, Junguang Wu, Chunying Chen
Summary: The severe imbalance between supply and demand in organ resection or severe tissue damage poses life-threatening risks. Carbon nanotubes (CNTs) have emerged as promising platforms due to their unique properties. CNTs are commonly used as additives in tissue engineering scaffolds to modify structure, porosity, mechanical strength, and more. This review summarizes the applications and design strategies of CNTs in various tissue engineering fields and discusses their potential biosafety risks and solutions. Future developments of CNTs in tissue engineering are also speculated.
Review
Materials Science, Multidisciplinary
Shivesh Anand, Serena Danti, Lorenzo Moroni, Carlos Mota
Summary: It is estimated that by 2050 one in every ten people will be suffering from disabling hearing loss. Perforated tympanic membranes (TMs) are the most common injury to the human ear, resulting in a partial or complete hearing loss due to inept sound conduction. Tissue engineering approaches, including TM repair and reconstruction, have been developed for TM regeneration.
PROGRESS IN MATERIALS SCIENCE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Ellen Funkhouser, Jack L. Ferracane, Thomas J. Hilton, Valeria V. Gordan, Gregg H. Gilbert, Rahma Mungia, Vanessa Burton, Cyril Meyerowitz, Dorota T. Kopycka-Kedzierawski
Summary: This study found that treatment resolved the majority of pain associated with a cracked tooth. For untreated cracked teeth, although the resolution degree of biting pain, spontaneous pain, and cold pain was not as high as that of treated cracked teeth, most of the pain problems were relieved during the follow-up study.
JOURNAL OF DENTISTRY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Hyun-Jung Kim, Jack L. Ferracane, MinJoo Park, Kyoung-Kyu Choi
Summary: This study investigated the use of an ethyl cellulose coating as an evaluation agent to mitigate the effects of saliva contamination on the bond strength of zirconia restorations. The results showed that the ethyl cellulose coating provided better protection against saliva contamination compared to other cleaning methods for the bonding surface of zirconia restorations.
JOURNAL OF PROSTHETIC DENTISTRY
(2022)
Editorial Material
Dentistry, Oral Surgery & Medicine
Richard B. Price, Jack L. Ferracane, Brian W. Darvell, Jean-Francois Roulet
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Ningcheng Li, Jack Ferracane, Nicole Andeen, Steven Lewis, Randy Woltjer, Sandra Rugonyi, Younes Jahangiri, Barry Uchida, Khashayar Farsad, John A. Kaufman, Ramsey Al-Hakim
Summary: This study characterized the impact of vein wall biomechanics on inflow diameter and luminal flow during venous angioplasty and stent placement. The results showed that the postthrombotic vein had more pronounced inflow channel narrowing and increased areas of low wall shear rate compared to the healthy vein. These findings highlight the significance of postthrombotic venous properties in optimizing venous intervention outcomes.
JOURNAL OF VASCULAR AND INTERVENTIONAL RADIOLOGY
(2022)
Review
Engineering, Biomedical
Cristiane Miranda Franca, Gabriela de Souza Balbinot, Diana Cunha, Vicente de Paulo Aragao Saboia, Jack Ferracane, Luiz E. Bertassoni
Summary: Dental caries is a multifactorial and dynamic disease that affects over 90% of adults worldwide. The current treatment for decayed tissue involves using materials to replace lost enamel or dentin. The development and understanding of restorative dental materials rely on in-vitro and in-vivo tests. This review discusses the unique characteristics of the tooth and dentin-pulp interface for material testing, commonly used in-vitro tests for evaluating dental material biocompatibility, and presents future directions for biological research on dental materials using tissue engineering and organs on-a-chip approaches.
ACTA BIOMATERIALIA
(2022)
Review
Dentistry, Oral Surgery & Medicine
Lorenzo Tavelli, Shayan Barootchi, Giulio Rasperini, William Giannobile
Summary: Scientific advancements in biomaterials, cellular therapies, and growth factors have brought new therapeutic options for periodontal and peri-implant reconstructive procedures. These tissue engineering strategies have been proven to be safe and effective in periodontal and peri-implant reconstruction therapies.
PERIODONTOLOGY 2000
(2023)
Review
Dentistry, Oral Surgery & Medicine
Jack L. Ferracane, Thomas J. Hilton, Ellen Funkhouser
Summary: This article reviews a large observational study on posterior teeth with cracks, discussing clinical characteristics, treatment, and outcomes. The study found that while cracked teeth are prevalent, only a small percentage will fracture or show crack progression in a 3-year period. Characteristics that guide treatment decisions include active caries, biting pain, and detectable cracks. The main treatment option is a complete crown.
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION
(2023)
Article
Chemistry, Analytical
Sriram Kumar, Anh Tuan Nguyen, Subir Goswami, Jack Ferracane, Dipankar Koley
Summary: This study describes a noninvasive electrochemical impedance approach for real-time monitoring of complex microbial biofilms derived from dental plaque on a range of substrates. The method can be applied to glass, dental filling resin, and Ca2+-releasing resin composites. Changes in impedance can be used to monitor biofilm volume in real-time, and the technique can also measure local pH changes at the biofilm-substrate interfaces. This impedance-based method is a useful tool for tracking biofilm growth on various substrates.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Riccardo Di Gianfilippo, Chin-Wei Wang, Yuying Xie, Janet Kinney, James Sugai, William V. V. Giannobile, Hom-Lay Wang
Summary: This randomized clinical trial examined the changes in protein biomarker levels and bacterial profiles after surgical reconstructive therapy for peri-implantitis. The study also investigated the impact of adjunctive Er:YAG laser on protein biomarkers and microbial outcomes. The results showed that the surgical treatment significantly affected the levels of protein biomarkers and reduced the bacterial load.
CLINICAL ORAL IMPLANTS RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Lorenzo Tavelli, Shayan Barootchi, Maria Vera Rodriguez, Suncica Travan, Tae-Ju Oh, Rodrigo Neiva, William V. Giannobile
Summary: A 13-year follow-up was conducted to evaluate the long-term outcomes of living cellular construct (LCC) versus free gingival graft (FGG) for keratinized tissue width augmentation. The results showed that both LCC and FGG were effective in augmenting KTW, with similar stability in treatment outcomes from 6 months to 13 years. However, FGG had better clinical outcomes over 13 years, while LCC showed better esthetics and patient-reported outcomes.
JOURNAL OF PERIODONTOLOGY
(2023)
Editorial Material
Dentistry, Oral Surgery & Medicine
Donald B. Giddon, William Giannobile
JOURNAL OF THE AMERICAN DENTAL ASSOCIATION
(2023)
Article
Chemistry, Multidisciplinary
Ryan Schmidt, Matthew G. Logan, Sarah Patty, Jack L. Ferracane, Carmem S. Pfeifer, Alexander J. Kendall
Summary: A careful analysis of the behavior of two thiol quantification chromophores (Ellman's reagent and Aldrithiol-4) in nonaqueous solvents is presented, showing a wide range of kinetic profiles and response factors and a large variance for nonaqueous systems. Several robust benchtop and room-temperature methods using different organic solvents are reported and compared to aqueous conditions. The validation of analytical analyses in nonaqueous systems and the quantification of cysteine content in ovalbumin are also presented. This work serves as a treatise on the utilization of thiol-disulfide exchange chromophores under nonaqueous conditions for thiol quantification.
Article
Dentistry, Oral Surgery & Medicine
Mikihiro Kobayashi, Kiyono Koi, Sarah Wiskoski, Hidehiko Watanabe, Steven Lewis, Jack L. Ferracane
Summary: This study aimed to investigate the relationship between filler size and the surface properties of roughness and gloss in experimental resin-based composites (RBCs) containing uniform spherical fillers. It was found that RBCs with smaller fillers had higher gloss and lower surface roughness, while RBCs with larger fillers had lower gloss and higher surface roughness.
JOURNAL OF ESTHETIC AND RESTORATIVE DENTISTRY
(2023)
Correction
Dentistry, Oral Surgery & Medicine
Diana Cunha, Nayara Souza, Manuela Moreira, Nara Rodrigues, Paulo Silva, Cristiane Franca, Sivaporn Horsophonphong, Ashley Sercia, Ramesh Subbiah, Anthony Tahayeri, Jack Ferracane, Pamela Yelick, Vicente Saboia, Luiz Bertassoni
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Diana Cunha, Nayara Souza, Manuela Moreira, Nara Rodrigues, Paulo Silva, Cristiane Franca, Sivaporn Horsophonphong, Ashley Sercia, Ramesh Subbiah, Anthony Tahayeri, Jack Ferracane, Pamela Yelick, Vicente Saboia, Luiz Bertassoni
Summary: This study developed a 3D-printed microgel supplemented with dentin matrix molecules (DMM) as a novel regenerative strategy for dental pulp capping. The results showed that the microgel with DMM had favorable cellular and inflammatory pulp responses, leading to the formation of organized pulp tissue, tertiary dentin, and new blood vessels. Compared to traditional treatment methods, the microgel with DMM showed better outcomes in promoting dentin bridge formation and reducing pulp necrosis.
CLINICAL ORAL INVESTIGATIONS
(2023)
