Article
Biochemistry & Molecular Biology
Masaki Kinoshita, Satoru Yamada, Junichi Sasaki, Shigeki Suzuki, Tetsuhiro Kajikawa, Tomoaki Iwayama, Chiharu Fujihara, Satoshi Imazato, Shinya Murakami
Summary: PLAP-1 plays a crucial role in the periodontal ligament by inhibiting inflammatory responses and maintaining periodontal tissue homeostasis. Knockout of PLAP-1 leads to changes in periodontal ligament structures and accelerated bone loss in periodontitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Mechanics
Sergei Bosiakov
Summary: The periodontal ligament is a soft connective tissue between the tooth root and the alveolar bone, with viscoelastic properties. This study aims to analyze the viscoelastic behavior of the periodontal ligament in the case of asymmetric tooth root tipping and develop a corresponding model.
Article
Biochemistry & Molecular Biology
Alexey A. Ivanov, Tamara I. Danilova, Alla V. Kuznetsova, Olga P. Popova, Oleg O. Yanushevich
Summary: The regeneration of periodontal tissues is important for the treatment of periodontitis. This study investigated the osteogenic and odontogenic differentiation of periodontal progenitor and stem cells in decellularized tooth matrix (dTM) and periodontal ligament (dPDL) in both 2D and 3D culture conditions. The results showed that the combination of dTM and dPDL promoted both osteogenic and odontogenic differentiation of periodontal cells.
Article
Dentistry, Oral Surgery & Medicine
Sonntana Seang, Nichakorn Chenboonthai, Natnicha Nisaeh, Araya Teantongdee, Saharat Jamsai, Chanunchida Changgnam, Kanika Yoongkiew, Somchai Yodsanga, Paksinee Kamolratanakul, Sermporn Thaweesapphithak, Thantrira Pornthaveetus, Vincent Everts, Thanaphum Osathanont, Chalida Nakalekha Limjeerajarus
Summary: This study evaluated the effects of iloprost as a root surface treatment agent on periodontal ligament regeneration and mineralization. The results showed that iloprost promoted periodontal ligament regeneration, reduced root resorption, and stimulated acellular cementum deposition. In vitro experiments also demonstrated that iloprost increased hPDLC mineralization through activation of the PGI(2) receptor.
JOURNAL OF ENDODONTICS
(2022)
Article
Endocrinology & Metabolism
Elis J. Lira dos Santos, Amanda B. de Almeida, Michael B. Chavez, Cristiane R. Salmon, Luciana S. Mofatto, Mariana Barbosa Camara-Souza, Michelle H. Tan, Tamara N. Kolli, Fatma F. Mohamed, Emily Y. Chu, Pedro Duarte Novaes, Eduardo C. A. Santos, Kamila R. Kantovitz, Brian L. Foster, Francisco H. Nociti Jr
Summary: Cementum, a mineralized tissue that covers tooth roots, and cementocytes, cells within cellular cementum, are hypothesized to play a key role in orthodontic tooth movement. Experimental results showed that during tooth movement, cementocytes undergo apoptosis and lead to root resorption, while also exhibiting increased cellular activity. Proteomic analysis revealed downregulation of certain extracellular matrix proteins during orthodontic tooth movement, along with differential expression of proteins in OTM sites compared to control samples.
Article
Biotechnology & Applied Microbiology
Haojie Fu, Lin Sen, Fangqi Zhang, Sirui Liu, Meiyue Wang, Hongyan Mi, Mengzhe Liu, Bingyan Li, Shumin Peng, Zelong Hu, Jingjing Sun, Rui Li
Summary: This study constructed xenogeneic bioengineered tooth roots and used extracellular vesicles from human adipose-derived mesenchymal stem cells (hASC-EVs) to protect the bio-roots from oxidative damage. The results showed that hASC-EVs reduced cell apoptosis and oxidative stress, improved cell proliferation and antioxidant capacity, and suppressed oxidative damage through the activation of the PI3K/Akt and NRF2 pathways. In vivo experiments demonstrated that hASC-EV administration enhanced the antioxidant effect of the bio-root and promoted the regeneration of periodontal ligament-like tissue.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Christian Behm, Michael Nemec, Fabian Weissinger, Marco Aoqi Rausch, Oleh Andrukhov, Erwin Jonke
Summary: The systematic review revealed that orthodontic forces induce increased expression levels of MMPs and TIMPs in the PDL during orthodontic tooth movement. However, the exact effect may vary depending on intervention and sample parameters as well as subject characteristics.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nanoscience & Nanotechnology
Yongxi Liang, Ajay Shakya, Xiaohua Liu
Summary: This study designed and fabricated a bioinspired tubular 3D matrix to guide the migration and growth of human periodontal ligament stem cells (PDLSCs) and form aligned fiber bundles. The tubular matrix controlled cell migration and alignment through inhibiting osteogenic differentiation. Mechanism study revealed involvement of the Yap1/Twist1 signaling pathway.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Physiology
Lay Thant, Masaru Kaku, Yoshito Kakihara, Masaru Mizukoshi, Megumi Kitami, Moe Arai, Kohei Kitami, Daiki Kobayashi, Yutaka Yoshida, Takeyasu Maeda, Isao Saito, Katsumi Uoshima, Makio Saeki
Summary: The periodontal ligament (PDL) is a specialized tissue that supports the tooth and is crucial for oral functions. This study investigated the tissue characteristics and protein composition of mouse molar PDL using advanced techniques. It revealed the impact of mechanical loading on PDL and identified potential regulatory networks.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Oncology
Valen Zhuoyou Yu, Shan Shan So, Maria Li Lung
Summary: This study investigated the oncogenic mechanism of p53 gene mutations in esophageal squamous cell carcinoma patients, revealing a novel GoF mechanism through which specific p53 mutants exhibit oncogenic features and contribute to ESCC tumorigenesis.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Shai Yona, Oded Medina, Rachel Sarig, Nir Shvalb
Summary: Orthodontic tooth movement is a topic of interest for both medical and engineering communities. Previous studies focused on stress distribution and remodeling processes, but lacked a practical clinical decision support platform. This paper presents a simplified numerical model that bridges this gap and provides accurate clinical results, as demonstrated by comparison with real-world cases.
APPLIED SCIENCES-BASEL
(2023)
Article
Chemistry, Multidisciplinary
Junyu Hou, Zuohui Xiao, Zengqian Liu, Hewei Zhao, Yankun Zhu, Lin Guo, Zhefeng Zhang, Robert O. Ritchie, Yan Wei, Xuliang Deng
Summary: The development of a bioinspired peri-implant ligament with enhanced osteointegration and energy-dissipation capabilities presents new possibilities for creating high-performance implanted materials with increased lifespans. This artificial ligament, based on a polymer-infiltrated, amorphous, titania nanotube array, increases bone-implant contact and reduces effective stress transfer from implant to peri-implant bone, achieving results not previously seen with titanium implants.
ADVANCED MATERIALS
(2021)
Article
Anatomy & Morphology
Alexandre Veronez, Leticia Albertini Pires, Andrea Aparecida de Aro, Maria Esmeria Corezola do Amaral, Marcelo Augusto Marretto Esquisatto
Summary: The study found that physical exercise in water has a positive effect on the structural organization and chemical composition of fibrocartilage in the tendons of diabetic rats, helping to preserve tendon structure.
Article
Cell Biology
Maria Pilar Solis-Hernandez, Carla Martin, Beatriz Garcia, Natalia Perez-Lopez, Yolanda Garcia-Mesa, Sara Gonzalez-Fernandez, Olivia Garcia-Suarez, Jesus Merayo, Ivan Fernandez-Vega, Luis M. Quiros
Summary: The study found that SLRPs exhibit different transcription and protein expression changes in CCs, which are related to patient survival data. There are distinct patterns of SLRP alterations in right- and left-sided colon tumors, with more deregulations observed in LSCCs. The research suggests that SLRPs may impact cell proliferation and serve as new differential markers for RS and LSCCs.
Article
Engineering, Environmental
Rui Wang, Li Liao, Xiaojun Huang, Shixing Ma, Shuang Wang, Hu Qiao, Hong Zhou, Jing Zou, Xiaoxia Su
Summary: Mechanical stimulation can enhance the osteogenic properties of exosomes secreted by PDLSCs through the upregulation of miR-200b/c. These miRNAs target Smurf1 to activate the BMP-Smad signaling pathway and promote osteogenic differentiation of mandibular BMSCs. Functionalized exosomes containing miR-200b/c can effectively promote the repair of alveolar bone defects in a rat model, providing a novel therapeutic approach for inflammatory bone loss in periodontitis.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Dentistry, Oral Surgery & Medicine
Priscila A. Giovani, Luciane Martins, Cristiane R. Salmon, Luciana S. Mofatto, Adriana F. P. Leme, Regina M. Puppin-Rontani, Tamara N. Kolli, Brian L. Foster, Francisco H. Nociti, Kamila R. Kantovitz
Summary: Proteomic analysis of dental cementum revealed clear differences between deciduous and permanent teeth, providing new insights into developmental disparities and physiological/pathological events such as root resorption. Studies showed distinct protein composition in deciduous and permanent dental cementum, highlighting potential implications for dental attachment and pathology.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Review
Developmental Biology
Natalie L. Andras, Fatma F. Mohamed, Emily Y. Chu, Brian L. Foster
Summary: This review discusses recent research on the precise regulation of the boundaries between the hard and soft tissues in the periodontium, particularly the interfaces of cementum, periodontal ligament, and alveolar bone, as well as the mechanisms involved in maintaining the periodontal ligament in its unmineralized state.
Article
Dentistry, Oral Surgery & Medicine
Maria Eduarda Scordamaia Lopes, Camila Chierici Marcantonio, Rafael Scaf de Molon, Paulo Sergio Cerri, Cristiane Ribeiro Salmon, Luciana Souto Mofatto, Francisco Humberto Nociti Junior, James Deschner, Joni Augusto Cirelli, Andressa Vilas Boas Nogueira
Summary: The study found that obesity did not significantly impact bone loss resulting from periodontitis, but did affect the proteome of periodontal ligament tissues in experimental periodontitis, potentially identifying targets for personalized approaches.
JOURNAL OF PERIODONTAL RESEARCH
(2022)
Article
Endocrinology & Metabolism
Fatma F. Mohamed, Michael B. Chavez, Shannon Huggins, Joshua Bertels, Alyssa Falck, Larry J. Suva, Brian L. Foster, Dana Gaddy
Summary: The researchers created a gene-edited large-animal model of hypophosphatasia (HPP) in sheep using CRISPR/Cas9 technology and found that the model faithfully reproduced the dentoalveolar defects observed in HPP patients. This provides a valuable translational model for studying the etiology and developing novel therapies for HPP.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Dentistry, Oral Surgery & Medicine
Henrique B. Abdalla, Rayssa R. Marchioro, Karen Elizabeth A. Galvao, Lucas N. Teixeira, Kamila R. Kantovitz, Ana Luiza G. M. Millas, Francisco H. Nociti Jr
Summary: The study demonstrated that PCL scaffolds have the potential to promote mineralization by supporting cementoblast adhesion, proliferation, and modulating the expression of key markers. This suggests a potential clinical application of PCL scaffolds in promoting new cementum formation.
JOURNAL OF PERIODONTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Taishi Komori, Hai Pham, Priyam Jani, Sienna Perry, Yan Wang, Tina M. Kilts, Li Li, Marian F. Young
Summary: This study examined the role of Type VI collagen in oral tissues using a Col6 alpha 2-KO mouse model. The results showed that Type VI collagen has a regulatory role in biomineralization in alveolar bone and changes in the ECM of alveolar bone could be associated with greater bone loss due to periodontitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Andressa V. B. Nogueira, Maria Eduarda S. Lopes, Camila C. C. Marcantonio, Cristiane R. R. Salmon, Luciana S. S. Mofatto, James Deschner, Francisco H. H. Nociti-Junior, Joni A. A. Cirelli
Summary: This study aimed to evaluate the effects of obesity on the proteomic profile of the periodontal ligament in rats induced with a high-fat diet. The results showed differential expression of proteins between the obese and control groups, which were associated with obesity, suggesting that the periodontal ligament is responsive to obesity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
M. B. Chavez, M. H. Tan, T. N. Kolli, C. Zachariadou, F. Farah, F. F. Mohamed, E. Y. Chu, B. L. Foster
Summary: Bone sialoprotein (BSP) is a critical factor for alveolar bone healing, and its absence disrupts multiple major pathways involved in appropriate healing.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Xiangxiang Hu, Sing-Wai Wong, Kaixin Liang, Tai-Hsien Wu, Sheng Wang, Lufei Wang, Jie Liu, Mitsuo Yamauchi, Brian L. Foster, Jenny P. -Y. Ting, Baohong Zhao, Henry C. Tseng, Ching-Chang Ko
Summary: Degenerative diseases affecting the nervous and skeletal systems, such as neurodegenerative diseases and Paget's disease of bone, are influenced by the protein OPTN. OPTN and NRF2 show an age-related increase, and OPTN can interact with NRF2 and KEAP1 to up-regulate antioxidant response. Deletion of OPTN leads to increased oxidative stress and osteoclastogenesis, which can be attenuated by curcumin. These findings suggest the therapeutic potential of antioxidants for PDB.
Article
Endocrinology & Metabolism
Flavia Amadeu de Oliveira, Fatma F. F. Mohamed, Yuka Kinoshita, Sonoko Narisawa, Colin Farquharson, Koichi Miyake, Brian L. Foster, Jose Luis Millan
Summary: In this preclinical study, gene therapy using AAV8-TNAP-D-10 was found to improve skeletal phenotype in mouse models of adult HPP and pseudo-HPP. This finding contributes to the advancement of gene therapy for heritable forms of osteomalacia.
Article
Dentistry, Oral Surgery & Medicine
Christina Zachariadou, Amelia Anh Tran, Fuad Christopher Farah, Stormi Okerman Winter, Kimberly Jan Hammersmith, Brian Lee Foster
INTERNATIONAL JOURNAL OF PAEDIATRIC DENTISTRY
(2023)
Article
Physiology
Reut Shainer, Vardit Kram, Tina M. Kilts, Li Li, Andrew D. Doyle, Inbal Shainer, Daniel Martin, Carl G. Simon, Jinyang Zeng-Brouwers, Liliana Schaefer, Marian F. Young, Genomics and Computational Biol Core
Summary: Biglycan is a small proteoglycan found in the extracellular matrix and plays an important role in bone development. Our study reveals that biglycan is involved in osteoblast maturation during embryonic development, affecting bone integrity and strength. Deletion of the biglycan gene reduces the inflammatory response after fracture, leading to impaired periosteal expansion and callus formation. Additionally, biglycan is crucial for the cartilage phase preceding bone formation, as its absence leads to accelerated bone development with high levels of osteopontin, which negatively impacts bone structural integrity.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
Ana Mara da Silva Morais, Ysadora Mayara Reali Pereira, Isaac Jordao de Souza-Araujo, Daniel Furtado Silva, Vanessa Gallego Arias Pecorari, Orisson Ponce Gomes, Francisco Humberto Nociti-Junior, Regina Maria Puppin-Rontani, Waldemir Francisco Vieira-Junior, Paulo Noronha Lisboa-Filho, Kamila Rosamilia Kantovitz
Summary: This in vitro study investigated the potential impact of titanium dioxide nanotubes (TiO2-nts) on the performance of glass ionomer cement (GIC). The results showed that GIC reinforced with TiO2-nts had similar elemental composition to unblended GIC, but significantly reduced the release rate of aluminum. These findings contribute to our understanding of the effects of TiO2-nts on conventional GICs.
BRAZILIAN ORAL RESEARCH
(2022)
Article
Endocrinology & Metabolism
Taishi Komori, Youngmi Ji, Hai Pham, Priyam Jani, Tina M. Kilts, Vardit Kram, Li Li, Marian F. Young
Summary: This study investigates the role of type VI collagen in temporomandibular joint (TMJ) cartilage and finds that it is crucial for endochondral ossification in the TMJ. Alpha 2(VI) deficiency leads to abnormal chondrocyte differentiation and maturation, and alters gene expression profiles related to cell cycle and extracellular matrix (ECM) organization. The study also suggests deregulation of bone morphogenetic protein 2 (BMP2) activity during chondrocyte differentiation.
Article
Dentistry, Oral Surgery & Medicine
Isaac Jordao de Souza Araujo, Mariana Gallante Ricardo, Orisson Ponce Gomes, Priscila Alves Giovani, Julia Puppin-Rontani, Vanessa Arias Pecorari, Elizabeth Ferreira Martinez, Marcelo Henrique Napimoga, Francisco Humberto Nociti Junior, Regina Maria Puppin-Rontani, Paulo Noronha Lisboa-Filho, Kamila Rosamilia Kantovitz
Summary: This in vitro study demonstrated that incorporating TiO2 nanotubes into glass ionomer cement can enhance its antibacterial properties and affect Streptococcus mutans viability and the expression of key genes.
BRAZILIAN ORAL RESEARCH
(2021)