Article
Endocrinology & Metabolism
Xi Chen, Enrique Maldonado, Ralph A. DeFronzo, Devjit Tripathy
Summary: The study revealed that there is a relationship between plasma glucagon levels and insulin sensitivity and insulin secretion in obese individuals. As glucose intolerance worsens, the insulin-mediated suppression of glucagon secretion is impaired, which corresponds to the severity of insulin resistance and beta-cell dysfunction.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Multidisciplinary Sciences
Ruiling Liu, Cuilian Liu, Xiaozhen He, Peng Sun, Bin Zhang, Haoran Yang, Weiyun Shi, Qingguo Ruan
Summary: In this study, researchers investigate the role of miR-21 in the regulation of pancreatic beta cell function. They find that miR-21 promotes glucose-stimulated insulin secretion by enhancing glucose uptake and up-regulating Glut2 expression. They also demonstrate that delivering miR-21 into the pancreas can decrease blood glucose levels in diabetic mice.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Michele Massimino, Laura Sciacca, Nunziatina Laura Parrinello, Nunzio Massimo Scalisi, Antonino Belfiore, Riccardo Vigneri, Paolo Vigneri
Summary: IR isoforms exhibit different effects on cell proliferation and survival. While both IR-A and IR-B can inhibit cell proliferation when unbound to insulin, IR-B is more effective in reducing resistance to DNA damage. In the presence of insulin, IR-A provides a selective growth advantage over IR-B, particularly at high concentrations. Insulin-bound IR-A, but not IR-B, can protect cells from cytotoxicity induced by DNA damage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, Research & Experimental
Robson Barth, Carolina Ruoso, Sandra Mara Ferreira, Francieli Caroline de Ramos, Fernanda Barbosa Lima, Antonio Carlos Boschero, Gustavo Jorge dos Santos
Summary: Research findings indicate that DEX-induced proliferation of pancreatic beta cells relies on HNF4 alpha, leading to the expansion of beta-cell mass. Therefore, HNF4 alpha may serve as a potential therapeutic target for diabetes treatment.
Article
Medicine, Research & Experimental
Renata Spezani, Thatiany Souza Marinho, Luiz E. Macedo Cardoso, Marcia Barbosa Aguila, Carlos Alberto Mandarim-de-Lacerda
Summary: Obesity and type 2 diabetes mellitus cause morphofunctional alterations in pancreatic islet alpha and beta cells. A new GLP-1/Glucagon receptor dual agonist cotadutide may benefit islet cell arrangement and function. The study found that cotadutide led to weight loss, reduced insulin resistance, and improved markers of transdifferentiation, proliferation, apoptosis, and ER stress in obese mice.
Review
Nutrition & Dietetics
Nella Polidori, Eleonora Agata Grasso, Francesco Chiarelli, Cosimo Giannini
Summary: The impaired plasmatic levels of BCAAs in obese children and adolescents are associated with obesity-related metabolic disorders, potentially through the activation of the mTORC1 complex causing insulin resistance, and the accumulation of toxic metabolites leading to mitochondrial dysfunction and damage to pancreatic cells. These compounds may help in the early identification of complications related to pediatric obesity, but further studies are needed.
Article
Endocrinology & Metabolism
Lixia Lv, Xiumin Wang, Jinhua Shen, Ying Cao, Qin Zhang
Summary: This study found that miR-574-3p suppresses glucose toxicity-induced pancreatic beta-cell dysfunction by targeting PRMT1. The high expression of PRMT1 was associated with reduced proliferation, insulin secretion, and GLUT1 level, as well as increased apoptosis, AGEs, and ROS levels in pancreatic beta cells.
DIABETOLOGY & METABOLIC SYNDROME
(2022)
Article
Biochemistry & Molecular Biology
Jing Wang, Qingchen Qiao, Yaxi Sun, Wenting Yu, Jiran Wang, Minjia Zhu, Kai Yang, Xiaofeng Huang, Yuxing Bai
Summary: This study investigated the impact of cell culture density on stem cell proliferation, osteoblastogenesis, and regulation. The results showed that the proliferation rate of human periodontal ligament stem cells (hPDLSCs) decreased with an increase in initial plating density. After osteogenic differentiation, hPDLSCs initially plated at a density of 2 x 10(4) cells/cm(2) showed the highest expression of OPG and RUNX2, as well as the highest OPG/RANKL ratio and cell calcium concentration. Furthermore, exosomes derived from hPDLSCs cultured at different initial cell densities were found to regulate osteoblastic differentiation of human bone marrow stromal cells (hBMSC), with the 2 x 10(4) cells/cm(2) initial cell density group showing the highest expression of OPG, OCN, RUNX2, and osterix, as well as the highest average calcium concentration. This provides a new perspective for the clinical application of stem cell osteogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Romina Mehdizadeh, Alireza Madjid Ansari, Flora Forouzesh, Fatemeh Shahriari, Seyed Peyman Shariatpanahi, Ali Salaritabar, Mohammad Amin Javidi
Summary: The average survival of patients with glioblastoma is 12-15 months, making it crucial to find new treatment methods, especially for cases resistant to current therapies. This research investigates the antitumor effect of extremely low-frequency electromagnetic fields (ELF-EMF) on specific glioblastoma cell lines. The findings suggest that ELF-EMF induces apoptosis and regulates cell cycle progression, potentially offering a low-side effect cancer treatment option.
SCIENTIFIC REPORTS
(2023)
Article
Immunology
Hui Zhou, Xiyan Liao, Qin Zeng, Haowei Zhang, Jianfeng Song, Wanyu Hu, Xiaoxiao Sun, Yujin Ding, Dandan Wang, Yalun Xiao, Tuo Deng
Summary: Accumulation and activation of immunocytes in adipose tissues are important in obesity-induced inflammation and insulin resistance. Chemokines, especially CCL5, play a crucial role in recruiting immunocytes to adipose tissue during obesity. CCL5 deficiency exacerbates adipose inflammation and impairs insulin sensitivity in obese mice. This study provides insights into the role of CCL5 in obesity-induced adipose inflammation.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Daehwan Choi, Takenobu Ishii, Munetada Ishikawa, Tomohisa Ootake, Hirokazu Kamei, Kohei Nagai, Kenji Sueishi
Summary: This study investigated the impact of osteoblast vibration on cell cycle, differentiation, and aging. Primary maxilla osteoblasts were subjected to vibration at different frequencies, and the results showed that cell proliferation increased significantly at 30 and 300 Hz. Differentiation of osteoblasts was also enhanced at 30 Hz, while markers of aging were activated. Overall, providing optimal vibration to osteoblasts promotes cell cycle progression and differentiation, but also induces cell aging.
Article
Cell Biology
Min Tan, Yanxiang Cheng, Xiaozhu Zhong, Dongyong Yang, Sushi Jiang, Yang Ye, Miao Ding, Guijun Guan, Dongzi Yang, Xiaomiao Zhao
Summary: The study revealed that LNK was higher in the PCOS group compared to the control group, positively correlated with granulosa cell apoptosis and insulin resistance, and negatively correlated with follicle maturation rate. Overexpression of LNK in KGN cells induced the AKT/FOXO3 signaling pathway in response to insulin, leading to FOXO3 translocation to the nucleus and promotion of granulosa cell apoptosis. LNK knockout partially restored estrous cycle and improved glucose metabolism in PCOS mice.
Article
Biochemistry & Molecular Biology
Khaoula Errafii, Said Boujraf, Mohamed Chikri
Summary: Understanding the role of white adipose tissue (WAT) in metabolic syndrome, including obesity and type 2 diabetes (T2D), is important. Insulin resistance plays a key role in the development of T2D. The comparison of WAT differences between obese diabetics and obese individuals with normal glucose tolerance and insulin resistance can provide insights into the molecular pathways underlying the development of obesity-related T2D.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medicine, Research & Experimental
Camila O. de Souza, Vivian A. Paschoal, Xuenan Sun, Lavanya Vishvanath, Qianbin Zhang, Mengle Shao, Toshiharu Onodera, Shiuhwei Chen, Nolwenn Joffin, Lorena M. A. Bueno, Rana K. Gupta, Da Young Oh
Summary: This study reveals that the lack of GPR92 in islet macrophages can lead to beta cell dysfunction, disrupting glucose homeostasis. Additionally, stimulation of GPR92 inhibits islet inflammation and increases insulin secretion.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Article
Pharmacology & Pharmacy
Neveen A. Hussein, Samia A. Ebied, Hoda A. Nour, Usama K. Zaki, Sahar M. EL-Kotishy, Tarek M. Salem
Summary: This study utilized metabolomics technique to investigate the response to liraglutide treatment and its effects on AcylCNs and insulin sensitivity. The results showed that liraglutide treatment led to weight loss, increased levels of C0 and C3, and decreased values of other parameters, with no impact on creatinine levels.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Chayodom Maneechote, Sasiwan Kerdphoo, Thidarat Jaiwongkam, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: Chronic inhibition of mitochondrial fission and promotion of fusion have cardioprotective effects in pre-diabetic rats with cardiac ischemia-reperfusion injury, by relieving cardiac mitochondrial dysfunction and dynamic alterations, and reducing myocardial infarction, thereby improving cardiac function.
CARDIOVASCULAR DRUGS AND THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Chanisa Thonusin, Patcharapong Pantiya, Natticha Sumneang, Titikorn Chunchai, Wichwara Nawara, Busarin Arunsak, Natthaphat Siri-Angkul, Sirawit Sriwichaiin, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: Short-term caloric restriction or exercise followed by weight maintenance can improve cardiometabolic health in prediabetes, and high baseline CRF is associated with favorable cardiac and metabolic profiles.
MOLECULAR MEDICINE
(2022)
Review
Pharmacology & Pharmacy
Panat Yanpiset, Chayodom Maneechote, Sirawit Sriwichaiin, Natthaphat Siri-Angkul, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: Cardiomyocyte death plays a major role in the development of myocardial infarction and myocardial ischemia/reperfusion injury. Pyroptosis, a regulated programmed cell death pathway, has been shown to be involved in these conditions. Activation of gasdermin D (GSDMD) through the canonical or non-canonical pathways is an important step in pyroptosis. Inhibition of GSDMD provides cardioprotection against myocardial infarction and myocardial ischemia/reperfusion injury.
ACTA PHARMACEUTICA SINICA B
(2023)
Article
Dentistry, Oral Surgery & Medicine
Savitri Vaseenon, Tanida Srisuwan, Nipon Chattipakorn, Siriporn C. Chattipakorn
Summary: This study aimed to investigate the effects of lipopolysaccharides (LPS), hydrogen peroxide (H2O2), and their combination on various indicators of human dental pulpal cells. The results showed that H2O2 and the combined agents equally decreased cell proliferation. LPS, H2O2, and the combination decreased mineralization and differentiation while increasing inflammation and apoptosis. Surprisingly, LPS and the combined agents also increased expression of SOD2 and caused an imbalance in mitochondrial dynamics.
INTERNATIONAL ENDODONTIC JOURNAL
(2023)
Article
Toxicology
Chanisa Thonusin, Wichwara Nawara, Thawatchai Khuanjing, Nanthip Prathumsup, Apiwan Arinno, Benjamin Ongnok, Busarin Arunsak, Sirawit Sriwichaiin, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: This study investigated the changes in blood and cardiac metabolome levels following treatment with doxorubicin and trastuzumab, and their association with cardiac dysfunction and injury. The study found that there were distinct patterns of metabolome alterations between the two treatment groups, and that the constituents of the blood metabolome may serve as non-invasive biomarkers for assessing the severity and prognosis of heart failure induced by these drugs.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Pharmacology & Pharmacy
Weiwei Zhu, Minxiu Wang, Leiming Jin, Bin Yang, Bin Bai, Rumbidzai Natasha Mutsinze, Wei Zuo, Nipon Chattipakorn, Joo Young Huh, Guang Liang, Yi Wang
Summary: This study discovered a natural compound, licochalcone A, with anti-inflammatory activity. It was found to directly bind to the protein MD2, which regulates the LPS inflammatory signaling pathway, and blocked both TRIF- and MYD88-dependent pathways. In vivo experiments showed that licochalcone A treatment alleviated acute lung injury (ALI) in mice by reducing immunocyte infiltration and suppressing TLR4 pathway activation and inflammatory cytokine production.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Medicine, Research & Experimental
Chanon Piamsiri, Chayodom Maneechote, Kewarin Jinawong, Busarin Arunsak, Titikorn Chunchai, Wichwara Nawara, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: Myocardial infarction (MI) is a leading cause of death worldwide, and oxidative stress, inflammation, and mitochondrial dysfunction are recognized as possible mechanisms in the development of MI. Different types of programmed cell death (PCDs), including apoptosis, necroptosis, ferroptosis, and pyroptosis, have been associated with different phases of ischemic injury following MI. However, it is still unknown which types of PCDs play the most dominant role in post-MI.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Kwannapas Saengsin, Rekwan Sittiwangkul, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: Cardiovascular disease is the leading cause of death globally. Molecular hydrogen, which can be administered through various methods, has shown antioxidant, anti-inflammatory, and anti-apoptotic effects, providing protection for the heart.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Prit Kusirisin, Nattayaporn Apaijai, Kajohnsak Noppakun, Srun Kuanprasert, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: Contrast-induced acute kidney injury (CI-AKI) is a common type of acute kidney injury in hospitalized patients. However, diagnosing CI-AKI using serum creatinine may not be early enough. This study aimed to investigate the role of circulating mitochondrial dysfunction as a potential early biomarker for detecting CI-AKI. Twenty patients with chronic kidney disease (CKD) undergoing percutaneous coronary intervention (PCI) were included. Blood and urine samples were collected at different time points after PCI to measure neutrophil gelatinase-associated lipocalin (NGAL) levels and assess oxidative stress, inflammation, mitochondrial function, dynamics, and cell death. The results showed that 40% of patients developed AKI, and plasma NGAL levels increased after 24 hours of receiving contrast media. Cellular and mitochondrial oxidative stress, dysfunction, and decreased fusion occurred 6 hours after contrast media exposure. The subgroup with AKI had higher necroptosis cells and TNF-alpha mRNA expression compared to the subgroup without AKI. In conclusion, circulating mitochondrial dysfunction could serve as an early predictive biomarker for CI-AKI in CKD patients receiving contrast media. These findings provide insights for the development of novel strategies to prevent CI-AKI based on its pathophysiology.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2023)
Article
Endocrinology & Metabolism
Chanisa Thonusin, Wichwara Nawara, Apiwan Arinno, Thawatchai Khuanjing, Nanthip Prathumsup, Benjamin Ongnok, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: Using mass spectrometry-based targeted metabolomics, this study aimed to investigate the patterns of cardiac metabolic reprogramming and energetics in doxorubicin-induced heart failure, as well as the potential effects of melatonin on these processes. The results showed that co-treatment with melatonin could restore cardiac function, glucose and ketone body utilization, and adenosine triphosphate production, while also increasing cardiac fatty acid oxidation, branched-chain amino acid catabolism, and anaplerosis. These findings highlight the potential efficacy of melatonin in cardiac metabolic reprogramming and energetics, suggesting it as an adjunctive treatment for doxorubicin-induced heart failure.
JOURNAL OF PINEAL RESEARCH
(2023)
Review
Pharmacology & Pharmacy
Chayodom Maneechote, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: Doxorubicin is an effective chemotherapy drug, but its cardiotoxicity limits its effectiveness. Recent research has shown that excessive mitochondrial fission and impaired fusion contribute to this cardiotoxicity. However, using inhibitors of mitochondrial fission or promoters of fusion can protect the heart against the toxicity of doxorubicin.
Review
Environmental Sciences
Wanitcha Gumtorntip, Nuntana Kasitanon, Worawit Louthrenoo, Nipon Chattipakorn, Siriporn C. Chattipakorn
Summary: This review summarizes and discusses the association between air pollutants and the development or aggravation of rheumatoid arthritis (RA). The findings suggest that air pollutants can stimulate immunological processes and inflammatory mediators and autoantibodies productions. Furthermore, air pollutants can induce and aggravate RA disease activity. However, discrepancies in the results highlight the need for future studies to elucidate these mechanisms and identify reliable biomarkers for individual susceptibility and disease activity assessment.
ENVIRONMENTAL POLLUTION
(2023)
Article
Biochemistry & Molecular Biology
Thawatchai Khuanjing, Chayodom Maneechote, Benjamin Ongnok, Nanthip Prathumsap, Apiwan Arinno, Titikorn Chunchai, Busarin Arunsak, Siriporn C. Chattipakorn, Nipon Chattipakorn
Summary: This study found that donepezil protects against trastuzumab-induced cardiotoxicity by reducing mitochondrial dysfunction, oxidative stress, inflammation, and cardiomyocyte death, leading to improved left ventricular function through activation of muscarinic acetylcholine receptors. This provides a new intervention strategy for treating trastuzumab-induced cardiotoxicity.
MOLECULAR MEDICINE
(2023)
Review
Surgery
Kittitorn Supphapipat, Prangmalee Leurcharusmee, Nipon Chattipakorn, Siriporn C. Chattipakorn
Summary: Air pollution has negative effects on human health, especially for organ transplant patients receiving immunosuppressive treatment. Clinical investigations have shown that air pollution is associated with organ rejection and various diseases.
CLINICAL TRANSPLANTATION
(2023)
Article
Biochemistry & Molecular Biology
Worawat Songjang, Nitchawat Paiyabhroma, Noppadon Jumroon, Arunya Jiraviriyakul, Nitirut Nernpermpisooth, Porrnthanate Seenak, Sarawut Kumphune, Siriwan Thaisakun, Narumon Phaonakrop, Sittiruk Roytrakul, Panyupa Pankhong
Summary: Sepsis, characterized by dysregulated immune response and vascular endothelial cell injury, is a significant public health problem. This study explored the potential of DAMPs as novel biomarkers for early sepsis diagnosis. Using an in vitro model, proteomic profiling of specific DAMPs in vascular endothelial cells in response to early LPS stimulation was conducted, revealing potential protein candidates for early sepsis diagnosis.
