Article
Biochemistry & Molecular Biology
Hiroyuki Inoue, Hidetaka Kuroda, Wataru Ofusa, Sadao Oyama, Maki Kimura, Tatsuya Ichinohe, Yoshiyuki Shibukawa
Summary: The ionotropic P2X receptor P2X(7) is believed to play a role in regulating and generating nociceptive pain in neuropathological diseases. Detailed examination of its functional properties in trigeminal ganglion (TG) neurons revealed that P2X(7) may interact with other P2X receptors and pannexin-1 (PANX1) channels to influence pain sensing pathways through autocrine or paracrine signaling. The complex formed by P2X(7)-PANX1-P2X receptor/channel may be involved in long-lasting neuronal activity driven by extracellular high-concentration ATP following tissue damage in the orofacial area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Kajohnkiart Janebodin, Rakchanok Chavanachat, Aislinn Hays, Morayma Reyes Gil
Summary: The study aimed to investigate the role of VEGFR-2 in odontoblast differentiation of dental pulp stem cells (DPSCs) by knocking down the expression of VEGFR-2. The results showed that VEGFR-2 is crucial for odontoblast differentiation in DPSCs, and deficiency of VEGFR-2 may impair the differentiation of odontoblast cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Yoon Seon Lee, Yeoung-Hyun Park, Geumbit Hwang, Hyejin Seo, Si Hyoung Ki, Shengfeng Bai, Chul Son, Seong Min Roh, Su-Jin Park, Dong-Seol Lee, Ji-Hyun Lee, You-Mi Seo, Won Jun Shon, Daehyun Jeon, Mi Jang, Sahng G. Kim, Byoung-Moo Seo, Gene Lee, Joo-Cheol Park
Summary: Loss of Cpne7 accelerates cellular senescence in odontoblasts due to oxidative stress and DNA damage accumulation, leading to impaired survival and aberrant dentin formation. However, treatment with CPNE7-derived functional peptide or Cpne7 transgenic mice rescue the aged dental pulp phenotype.
Article
Neurosciences
Laurence Bourgeais-Rambur, Laurianne Beynac, Jean-Charles Mariani, Mickael Tanter, Thomas Deffieux, Zsolt Lenkei, Luis Villanueva
Summary: This study explored the links between neurovascular coupling and cortical craniofacial nociceptive activities modulated by spreading depolarization (SD). The findings suggest that SD triggers strong physiological and electrophysiological responses in S1, providing a useful preclinical tool for deciphering central sensitization mechanisms involved in migraine attacks.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Cell Biology
Monica Stanwick, Courtney Barkley, Rosa Serra, Andrew Kruggel, Amy Webb, Yue Zhao, Maciej Pietrzak, Chandler Ashman, Allie Staats, Shifa Shahid, Sarah B. Peters
Summary: Transforming growth factor beta (TGF beta) plays an important role in tooth morphogenesis and mineralization. Deletion of Tgfbr2 in dental pulp mesenchyme leads to significant defects in bone and teeth, including reduced mineralization and short roots. Tgfbr2 deletion disrupts axon-like structures and reduces innervation in teeth. Downregulation of mineralization-related and neuronal genes is observed in Tgfbr2-deleted dental pulp. Tgfbr2 in the dental pulp mesenchyme regulates differentiation and the ability to guide neurite outgrowth during tooth mineralization and innervation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
B. Michot, S. M. Casey, J. L. Gibbs
Summary: Mediators released by DPSCs, stimulated with CGRP, induce neuronal sensitization through the CXCR2 receptor, likely contributing to pain symptoms that develop in pulpitis.
JOURNAL OF DENTAL RESEARCH
(2021)
Review
Multidisciplinary Sciences
A. Golez, M. Ovsenik, K. Cankar
Summary: This study aimed to review the available evidence on the short and long-term effects of orthodontic tooth movement (OTM) on dental pulp sensitivity and identify clinically relevant risk factors. The findings suggest that OTM can negatively impact dental pulp sensitivity, with the type of OTM and patients' age identified as clinically relevant risk factors.
Article
Clinical Neurology
Can Sarica, Christian Iorio-Morin, David H. Aguirre-Padilla, Michelle Paff, Samuelle-Arianne Villeneuve, Artur Vetkas, Kazuaki Yamamoto, Nardin Samuel, Vanessa Milano, Aaron Loh, Brendan Santyr, Ajmal Zemmar, Andres M. Lozano, Mojgan Hodaie
Summary: This study systematically reviewed the literature on peripheral nerve field stimulation (PNFS) for trigeminal pain and found that PNFS can provide effective long-term pain control for patients with trigeminal pain.
JOURNAL OF NEUROSURGERY
(2022)
Article
Biochemistry & Molecular Biology
Jean Kwon, Young In Choi, Hang Joon Jo, Sang Hoon Lee, Han Kyu Lee, Heesoo Kim, Jee Youn Moon, Sung Jun Jung
Summary: The study confirms that PGE1 can induce pain in mice and enhances the excitability of trigeminal ganglion neurons through HCN channel facilitation. The research also identifies the target mechanism of PGE1 in nociceptive neurons, suggesting its potential as a therapeutic target for pain management under inflammatory conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Clinical Neurology
Fatemeh Owlia, Nazanin Mahmoudzade, Jalil Modaresi, Marzieh Abutorabi Zarchi
Summary: The study showed that MS patients had a significantly reduced response to the electric pulp test in their maxillary central incisors compared to healthy individuals. This suggests that individuals with MS may have neuropathy.
Article
Cell & Tissue Engineering
S. M. Ziauddin, Misako Nakashima, Hideto Watanabe, Michiyo Tominaga, Koichiro Iohara
Summary: This study compared the stem cell properties and pulp regenerative potential of DT-DPSCs and PT-DPSCs from the same individual. The results showed that DT-DPSCs exhibited higher expression of stem cell markers and angiogenic factors compared to PT-DPSCs. DT-DPSC-derived conditioned medium also promoted increased cell proliferation, migration, and angiogenesis. Furthermore, the regenerated pulp tissues in both DT-DPSCs and PT-DPSCs groups were similar. Therefore, DT-DPSCs could be a potential alternative to PT-DPSCs for pulp regenerative therapy.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Neurosciences
Yuhui Luo, Abbie Suttle, Qiaojuan Zhang, Peng Wang, Yong Chen
Summary: Orofacial pain, affecting millions of Americans, is influenced by transient receptor potential (TRP) ion channels. The orofacial sensory system differs from the spinal sensory system in pain processing and sensitivities. TRPs in trigeminally-mediated pain have not been extensively documented compared to spinally-mediated pain.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Adriana Della Pietra, Nikita Mikhailov, Rashid Giniatullin
Summary: It has been discovered that satellite glial cells (SGCs) supporting neuronal sensitization have mechanosensitivity and may trigger migraine pain. Using live calcium imaging and labeling techniques, researchers found that the specific Piezo1 agonist Yoda1 can induce calcium transients in trigeminal nociceptive neurons and SGCs, indicating the presence of functional Piezo1 channels in these cells. Additionally, they observed that Yoda1 can produce sustained labeling effects in cells using the fluorescent marker FM1-43, which can be used to monitor the activation of Piezo1 channels in the trigeminal nociceptive system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Behavioral Sciences
Yannick Logghe, Iris Smet, Ali Jerjir, Peter Verelst, Marieke Devos, Jean-Pierre Van Buyten
Summary: This report describes successful treatment of two patients with trigeminal neuropathy using Gasserian ganglion stimulation. In the first case, a 53-year-old woman experienced immediate pain relief and was able to stop all medication. In the second case, a 64-year-old man experienced improved pain and reduced medication dosages.
BRAIN AND BEHAVIOR
(2021)
Review
Dentistry, Oral Surgery & Medicine
Pedro Henrique Marks Duarte, Theodoro Weissheimer, Carolina Horn Troian Michel, Gabriel Barcelos So, Ricardo Abreu da Rosa, Marcus Vinicius Reis So
Summary: The aim of this study was to determine whether orthodontic movements of traumatized teeth could lead to dental pulp necrosis. Five relevant studies were included in the analysis, and it was found that teeth with a history of trauma involving periodontal tissues were more susceptible to pulp necrosis during orthodontic movements. However, further well-designed studies are needed to confirm these findings.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Medicine, Research & Experimental
Mary R. Adams, Christopher T. Moody, Jennifer L. Sollinger, Yevgeny Brudno
MOLECULAR PHARMACEUTICS
(2020)
Article
Cardiac & Cardiovascular Systems
Natalie R. Nielsen, Krsna Rangarajan, Lan Mao, Howard A. Rockman, Kathleen M. Caron
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2020)
Article
Multidisciplinary Sciences
Alicia C. Tagliatela, Stephanie C. Hempstead, Priya S. Hibshman, Max A. Hockenberry, Hailey E. Brighton, Chad Pecot, James E. Bear
SCIENTIFIC REPORTS
(2020)
Article
Oncology
Dimitri G. Trembath, Eric S. Davis, Shanti Rao, Evan Bradler, Angelica F. Saada, Bentley R. Midkiff, Anna C. Snavely, Matthew G. Ewend, Frances A. Collichio, Carrie B. Lee, Georgia-Sofia Karachaliou, Fatih Ayvali, David W. Ollila, Michal T. Krauze, John M. Kirkwood, Benjamin G. Vincent, Nana Nikolaishvilli-Feinberg, Stergios J. Moschos
Summary: The presence of high tumor-infiltrating lymphocytes (TILs) in patients undergoing craniotomy for melanoma brain metastases (MBM) before 2011 may have prognostic significance. Intratumoral hemorrhage is associated with tumor volume, high expression of basic fibroblast growth factor (bFGF), and high density of CD31+alpha SMA- blood vessels.
FRONTIERS IN ONCOLOGY
(2021)
Article
Genetics & Heredity
Kathryn M. Meurs, Brian G. Williams, Dylan DeProspero, Steven G. Friedenberg, David E. Malarkey, J. Ashley Ezzell, Bruce W. Keene, Darcy B. Adin, Teresa C. DeFrancesco, Sandra Tou
Summary: This study identifies a novel form of cardiomyopathy associated with ALMS1 in Sphynx cats. The presence of a specific mutation in the ALMS1 gene was positively associated with hypertrophic cardiomyopathy in affected cats. These findings suggest that genes involved in cardiac development and cell regulation, such as ALMS1, may play a role in familial hypertrophic cardiomyopathy.
ORPHANET JOURNAL OF RARE DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Hong Ma, Ziqing Liu, Yuchen Yang, Dong Feng, Yanhan Dong, Tiffany A. Garbutt, Zhiyuan Hu, Li Wang, Changfei Luan, Cynthia D. Cooper, Yun Li, Joshua D. Welch, Li Qian, Jiandong Liu
Summary: Single-cell transcriptomic analysis reveals the diversity and subpopulation distribution of non-cardiomyocytes during cardiac regeneration, with macrophages playing a key role in activating fibroblast and endothelial cell subpopulations. The study also identifies dynamic transitions between macrophage functional states and factors associated with mRNA processing and transcriptional regulation. A blueprint for interrogating the molecular and cellular basis of cardiac regeneration is provided.
Article
Immunology
Bryan E. McQueen, Avinash Kollipara, Clare E. Gyorke, Charles W. Andrews, Ashley Ezzell, Toni Darville, Uma M. Nagarajan
Summary: This study found that TREM-1,3 plays a significant role in regulating neutrophil migration and the pathogenesis process in the female mouse genital tract, ensuring appropriate immune responses during Chlamydia muridarum infection.
INFECTION AND IMMUNITY
(2021)
Correction
Engineering, Biomedical
Megan L. Settell, Nicole A. Pelot, Bruce E. Knudsen, Aaron M. Dingle, Andrea L. McConico, Evan N. Nicolai, James K. Trevathan, J. Ashley Ezzell, Erika K. Ross, Kenneth J. Gustafson, Andrew J. Shoffstall, Justin C. Williams, Weifeng Zeng, Samuel O. Poore, Luis C. Populin, Aaron J. Suminski, Warren M. Grill, Kip A. Ludwig
JOURNAL OF NEURAL ENGINEERING
(2021)
Article
Dentistry, Oral Surgery & Medicine
Ning Yu, Jinmei Zhang, Sherill T. Phillips, Steven Offenbacher, Shaoping Zhang
Summary: The study found that the expression of PKP2 in gingival biopsies of periodontitis patients was significantly lower compared to healthy controls. PKP2 is crucial for maintaining gingival epithelial barrier function, and P.gingivalis impairs epithelial barrier function by promoting PKP2 degradation.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Oncology
Georgia Sofia Karachaliou, Rached Alkallas, Sarah B. Carroll, Chongshan Caressi, Danny Zakria, Nirali M. Patel, Dimitri G. Trembath, Jennifer A. Ezzell, Guillaume J. Pegna, Paul B. Googe, Jonathan P. Galeotti, Fatih Ayvali, Frances A. Collichio, Carrie B. Lee, David W. Ollila, Margaret L. Gulley, Douglas B. Johnson, Kevin B. Kim, Ian R. Watson, Stergios J. Moschos
Summary: This study found that APC/CTNNB1 gene mutations are associated with worse prognosis and early brain metastases in stage IV melanoma. However, PD-1 inhibitor-based treatments provide comparable benefits to both mutated and wild-type patients with stage IV melanoma.
Article
Biotechnology & Applied Microbiology
Zheng Chai, Xintao Zhang, Amanda Lee Dobbins, Richard Jude Samulski, Elizabeth P. Merricks, Timothy C. Nichols, Chengwen Li
Summary: Glucocorticoids, such as dexamethasone, have different effects on transgene expression in AAV-transduced livers in different species. In mouse experiments, dexamethasone significantly increased transgene expression, while no similar effect was observed in hemophilia B dogs. This study provides valuable information for the rational application of dexamethasone in future clinical studies.
HUMAN GENE THERAPY
(2022)
Article
Gastroenterology & Hepatology
Joseph Burclaff, R. Jarrett Bliton, Keith A. Breau, Meryem T. Ok, Ismael Gomez-Martinez, Jolene S. Ranek, Aadra P. Bhatt, Jeremy E. Purvis, John T. Woosley, Scott T. Magness
Summary: This study utilizes single-cell transcriptomics to analyze the cellular function of the healthy adult human small intestine and colon. The findings reveal novel lineage marker genes, regional differences, and the response of cells to the environment and drugs. It advances our understanding of human intestinal physiology.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Medicine, Research & Experimental
Robert H. Lee, Dorsaf Ghalloussi, Gabriel L. Harousseau, Joseph P. Kenny, Patrick A. Kramer, Fabienne Proamer, Bernhard Nieswandt, Matthew J. Flick, Christian Gachet, Caterina Casari, Anita Eckly, Wolfgang Bergmeier
Summary: Platelet homeostasis relies on tight regulation of production and clearance, with Rap1 signaling crucial for both processes. Deficiency in Rasa3 causes macrothrombocytopenia in mice due to platelet clearance in the spleen and liver, rather than impaired thrombopoiesis. Inhibiting the Rap1/talin1/alpha(IIb)beta(3) integrin axis can restore platelet count and lifespan in Rasa3-mutant mice, suggesting a potential new mechanism of platelet clearance.
Meeting Abstract
Gastroenterology & Hepatology
Joseph Burclaff, Jarrett Bliton, Keith A. Breau, Meryem T. Ok, Ismael Gomez Martinez, Jolene S. Ranek, Aadra Bhatt, Jeremy E. Purvis, John T. Woosley, Scott Magness
Article
Physiology
Evan M. Zeitler, J. Charles Jennette, Jennifer E. Flythe, Ronald J. Falk, John S. Poulton
Summary: This study on zebrafish shows that a high-calorie diet, regardless of fat content, can lead to kidney pathology, but these changes can be reversed after 4 weeks of calorie restriction.
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
(2022)
