Article
Biochemistry & Molecular Biology
Die Hu, Qian Ren, Zhongcheng Li, Sili Han, Longjiang Ding, Ziqian Lu, Linglin Zhang
Summary: The mechanism of amelogenin-derived peptide QP5 in regulating enamel biomimetic remineralization is uncovered for the first time using a combination of experiments and computer simulation. QP5 inhibits spontaneous nucleation by adsorbing onto minerals through its two domains. The C-tail domain plays a significant role in the formation of enamel-like crystals. QP5 adsorbs onto enamel and captures Ca2+ from solution to promote enamel remineralization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Materials Science, Multidisciplinary
Shuhui Geng, Yaping Lei, Malcolm L. Snead
Summary: The study found that the ADP7 peptide partially replaced the function of the full-length amelogenin protein and its several protein isoforms, indicating that ADP7 has certain enamel-forming function. The protein structure-function relationships identified through in vitro assays can be deployed in whole model animals using CRISPR-Cas9 to validate the function of a minimal protein domain.
Article
Dentistry, Oral Surgery & Medicine
K. Isono, E. Takahashi, I Miyoshi, M. Tsuneto, M. Hikosaka-Kuniishi, T. Yamane, H. Yamazaki
Summary: The tooth is mainly composed of dentin and enamel, and identification of dentin-producing odontoblasts and enamel-producing ameloblasts using reporter techniques is crucial for studying tooth development and tissue engineering regeneration. By establishing transgenic mouse lines labeling odontoblasts and ameloblasts, researchers were able to track the expression patterns of related genes and observe changes in enamel volume and dentin mineral density, providing valuable insights for tooth development and regeneration research. Additionally, examining the expression of specific cell surface molecules in these mice allowed for easy distinction and isolation of odontoblasts and ameloblasts, contributing to further studies in differentiation processes.
JOURNAL OF DENTAL RESEARCH
(2021)
Review
Agriculture, Dairy & Animal Science
Marcello Mezzasalma, Elvira Brunelli, Gaetano Odierna, Fabio Maria Guarino
Summary: This article provides a comprehensive report on the presence of different types of polyploidy in tetrapods, with a particular focus on its genomic, evolutionary, and ecological diversity. It suggests that polyploidy is an important pathway of genomic evolution in tetrapods, occurring in most higher-taxa and displaying a variety of different forms, genomic configurations, and biological implications.
Article
Physiology
Youbin Zhang, Tianquan Jin, Weiying Zhu, Mirali Pandya, Gokul Gopinathan, Michael Allen, David Reed, Timothy Keiderling, Xiubei Liao, Thomas G. H. Diekwisch
Summary: This study reveals the intimate relationship between enamel matrix pH, enamel protein self-assembly, and enamel crystal growth. The highly acidic pH of the early enamel matrix is shown to be essential for enamel protein structure and self-assembly, apatite crystal growth, and protein interactions.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cayla A. Stifler, Hajime Yamazaki, Pupa U. P. A. Gilbert, Henry C. Margolis, Elia Beniash
Summary: Amelogenin phosphorylation plays crucial roles in controlling the structural, crystallographic, mechanical, and compositional characteristics of dental enamel.
JOURNAL OF STRUCTURAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mirali Pandya, Thomas G. H. Diekwisch
Summary: During enamel formation, the organic enamel protein matrix interacts with calcium phosphate minerals to form elongated, parallel, and bundled enamel apatite crystals of extraordinary hardness and biomechanical resilience. Enamel proteins such as amelogenin, ameloblastin, and enamelin are secreted by specialized cells called ameloblasts, which also facilitate calcium and phosphate ion transport towards the enamel layer. This process involves complex and dynamic interactions that result in regularly seeded apatite growth and parallel enamel crystal elongation.
JOURNAL OF STRUCTURAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gayathri Visakan, Jingtan Su, Janet Moradian-Oldak
Summary: Studies on animal models have shown that ameloblastin (AMBN) is involved in cell-matrix adhesion and cell polarization during enamel formation. In this study, an in vitro 3D cell culture model was developed to investigate the role of AMBN in cell polarization and morphology. The addition of AMBN to the culture matrix resulted in the clustering and elongation of ameloblast cell lines in a dose-dependent manner. AMBN plays a critical signaling role in the initiation of cell polarity by acting as a communicator between cell-cell and cell-matrix interactions.
Article
Dentistry, Oral Surgery & Medicine
X. Zhu, Z. Ma, F. Xie, J. Wang
Summary: The absence of Ash2l resulted in reduced H3K4me3 modification, leading to abnormal morphology of dental germ and impaired enamel and dentin formation. Ash2l knockout also disrupted the expression of crucial genes such as Shh and Trp63, leading to aberrant differentiation, proliferation, and apoptosis of the dental epithelium.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Chemistry, Analytical
Ana Maria Casas-Ferreira, Miguel del Nogal-Sanchez, Angel Esparza Arroyo, Javier Velasco Vazquez, Jose Luis Perez-Pavon
Summary: This article introduces two new fast methods for assigning biological sex to prehistoric human remains. Both methods are based on the analysis of sexually dimorphic amelogenin protein fragments in tooth enamel. The results show that these methods are effective and feasible, with the advantages of being fast, accurate, and cost-effective. They can significantly increase sample throughput and enable more laboratories to perform sex determination.
MICROCHEMICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Alexander L. Danesi, Dimitra Athanasiadou, Ahmad Mansouri, Alina Phen, Mehrnoosh Neshatian, James Holcroft, Johan Bonde, Bernhard Ganss, Karina M. M. Carneiro
Summary: Biomineralization is a crucial process in which organisms produce mineralized tissues, such as teeth, bones, and shells, composed of hierarchically organized hydroxyapatite crystals. Developing protein-based materials as artificial scaffolds for guiding hydroxyapatite growth is an attractive goal for designing ordered nanomaterials and tissue regeneration. Uniaxial hydroxyapatite growth on a nanoengineered amelogenin scaffold combined with amelotin may provide insights into the molecular mechanism of hydroxyapatite formation and mineralized tissue formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Yaru Li, Yiwei Li, Qinghua Bai, Mingzhu Wen, Dandan Ma, Yisha Lin, Jinpu Chu
Summary: The study explored the regulatory effect of recombinant amelogenin peptide TRAP on the remineralization of early enamel carious lesions. The results showed that TRAP can promote the remineralization of enamel lesions and reduce mineral loss. This study provides a promising biomaterial for the treatment of early enamel carious lesions.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Chemistry, Analytical
Sandra Diez-Garcia, Maria-Jesus Sanchez-Martin, Jose Manuel Amigo, Manuel Valiente
Summary: By combining synchrotron radiation-based techniques, such as infrared microspectroscopy and micro X-ray diffraction, with specific data mining, the limitations of assessing dental enamel remineralization have been overcome. A controlled ion release of structural ions, including Ca2+, PO43-, F-, and Zn2+, for fluorapatite, in the presence of amelogenin, has been proposed to remineralize the surface of etched teeth.
ANALYTICAL CHEMISTRY
(2022)
Editorial Material
Anthropology
Glendon J. Parker, Tammy Buonasera, Julia M. Yip, Jelmer W. Eerkens, Michelle Salemi, Blythe Durbin-Johnson, Randall Haas, Jane E. Buikstra, Haagen Klaus, David M. Rocke, Brett Phinney
Summary: Biomolecular sex estimation using genomic and proteomic methods is a valuable tool in bioarchaeology, but has limitations and challenges that need to be addressed. Proteomic sex estimation based on amelogenin protein fragments in enamel is sensitive and quantifiable. Structural variants of the Y-chromosome and low peptide signals in mass spectrometry may complicate sex estimation results.
JOURNAL OF ARCHAEOLOGICAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Garry W. Buchko, Sebastian T. Mergelsberg, Barbara J. Tarasevich, Wendy J. Shaw
Summary: This study investigated the structure of Amelogenin under different pH conditions using NMR experiments, revealing that its self-assembly form changes with pH variation, with the N-terminus playing a key role in intermolecular interactions.
Article
Biochemistry & Molecular Biology
S. Delgado, D. Deutsch, J. Y. Sire
JOURNAL OF MOLECULAR EVOLUTION
(2017)
Article
Physiology
Barbara Gasse, Megana Prasad, Sidney Delgado, Mathilde Huckert, Marzena Kawczynski, Annelyse Garret-Bernardin, Serena Lopez-Cazaux, Isabelle Bailleul-Forestier, Marie-Cecile Maniere, Corinne Stoetzel, Agnes Bloch-Zupan, Jean-Yves Sire
FRONTIERS IN PHYSIOLOGY
(2017)
Article
Evolutionary Biology
Jean-Yves Sire, Sidney C. Delgado, Marc Girondot
BMC EVOLUTIONARY BIOLOGY
(2008)
Article
Evolutionary Biology
Nathalie Le Roy, Benjamin Marie, Beatrice Gaume, Nathalie Guichard, Sidney Delgado, Isabelle Zanella-Cleon, Michel Becchi, Stephanie Auzoux-Bordenave, Jean-Yves Sire, Frederic Marin
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
(2012)
Article
Evolutionary Biology
Nathalie Assaraf-Weill, Barbara Gasse, Nawfal Al-Hashimi, Sidney Delgado, Jean-Yves Sire, Tiphaine Davit-Beal
JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION
(2013)
Article
Biochemistry & Molecular Biology
Nawfal Al-Hashimi, Jean-Yves Sire, Sidney Delgado
JOURNAL OF MOLECULAR EVOLUTION
(2009)
Article
Biochemistry & Molecular Biology
Jeremie Silvent, Jean-Yves Sire, Sidney Delgado
JOURNAL OF MOLECULAR EVOLUTION
(2013)
Article
Biochemistry & Molecular Biology
Nawfal Al-Hashimi, Anne-Gaelle Lafont, Sidney Delgado, Kazuhiko Kawasaki, Jean-Yves Sire
MOLECULAR BIOLOGY AND EVOLUTION
(2010)
Article
Biochemistry & Molecular Biology
Sidney Delgado, Nicolas Vidal, Geraldine Veron, Jean-Yves Sire
MOLECULAR PHYLOGENETICS AND EVOLUTION
(2008)
Article
Biochemistry & Molecular Biology
Jeremie Silvent, Marc Robin, Camila Bussola Tovani, Yan Wang, Fabrice Soncin, Sidney Delgado, Thierry Azais, Capucine Sassoye, Marie-Madeleine Giraud-Guille, Jean-Yves Sire, Nadine Nassif
Summary: The confinement of collagen suprafibrillar in bone plays a crucial role in the activity of biomimetic acidic calcium-binding polymers on apatite mineralization. The specificity of the 57-KD fragment of DMP1 in the regulation of mineralization is validated without phosphorylation. Transient apatite phases are only found using a high nonphysiological concentration of additives.
Article
Dentistry, Oral Surgery & Medicine
Beatrice Richard, Sidney Delgado, Philippe Gorry, Jean-Yues Sire
ARCHIVES OF ORAL BIOLOGY
(2007)
Article
Dentistry, Oral Surgery & Medicine
S. Delgado, M. Ishiyama, J. -Y. Sire
JOURNAL OF DENTAL RESEARCH
(2007)
Review
Biology
Tiphaine Davit-Beal, Hideki Chisaka, Sidney Delgado, Jean-Yves Sire
BIOLOGICAL REVIEWS
(2007)
Article
Dentistry, Oral Surgery & Medicine
Jean-Yves Sire, Sidney Delgado, Marc Girondot
EUROPEAN JOURNAL OF ORAL SCIENCES
(2006)