Article
Infectious Diseases
Fen Pan, Na Zhao, Wantong Zhao, Chun Wang, Yan Sun, Haomin Zhang, Juanxiu Qin, Qian Liu, Hong Zhang
Summary: This study demonstrated that the two MALDI-TOF MS systems could differentiate most VGS isolates, but with different identification performances. The Bruker Biotyper system tends to produce more misidentifications compared to the VITEK MS system.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Microbiology
Daiki Tanno, Kyoichi Saito, Kazutaka Ohashi, Masahiro Toyokawa, Yukio Yamadera, Hiroki Shimura
Summary: This study developed a highly sensitive method for screening Group B Streptococcus (GBS), called MALDI detection with analysis of peaks of TOF (MDAPT). The method can detect GBS directly from cultured broth, providing an alternative for GBS screening in pregnant women and contributing to the prevention of severe GBS infectious diseases in neonates.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Doaa Ghaith, Mai M. Zafer, Thoraya Hosny, May AbdElfattah
Summary: The study aimed to accurately identify Candida spp. from ICU patients using MALDI-TOF MS, revealing that misidentification of C. famata is common with traditional phenotypic methods, and Candida species show low antifungal resistance.
CURRENT MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Zhuohao Wang, Yu Zhou, Genglin Guo, Quan Li, Yanfei Yu, Wei Zhang, Martha Vives
Summary: This study reveals a strong correlation between mass spectra pattern and virulence phenotype among Streptococcus suis serotype 2 (SS2) for the first time. Machine learning-based classifiers were established and achieved an accuracy of over 90%. Overall, this study paves the way for rapid detection of virulent SS2 and provides new ideas for genotype-dependent detection of specific pathogens.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Microbiology
Fernando Sanchez-Juanes, Noelia Calvo Sanchez, Moncef Belhassen Garcia, Carmen Vieira Lista, Raul Manzano Roman, Rufino Alamo Sanz, Antonio Muro Alvarez, Juan Luis Munoz Bellido
Summary: Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is a simple, fast, and specific method used for bacterial identification, and has also been shown to be useful for identifying certain parasites and arthropod vectors. Fast, simple, and reliable methods for identifying arthropod vectors are crucial for controlling diseases transmitted by these vectors.
Review
Chemistry, Analytical
Hugo M. Santos, Luis B. Carvalho, Carlos Lodeiro, Goncalo Martins, Ines L. Gomes, Wilson D. T. Antunes, Vanessa Correia, Maria M. Almeida-Santos, Helena Rebelo-de-Andrade, Antonio P. A. Matos, J. L. Capelo
Summary: This work presents a tutorial on the capabilities of bioanalytical mass spectrometry in detecting and differentiating viruses at the peptide level, as well as providing diagnosis and prognosis for infected patients. It offers insights into the possibilities of current high-resolution mass spectrometry technology and bioinformatics.
MICROCHEMICAL JOURNAL
(2023)
Article
Microbiology
Eloise J. Busby, Ronan M. M. Doyle, Clara Leboreiro Babe, Kathryn A. A. Harris, Damien Mack, Gema Mendez-Cervantes, Denise M. M. O'Sullivan, Vicky Pang, Zahra Sadouki, Priya Solanki, Jim F. F. Huggett, Timothy D. D. McHugh, Emmanuel Q. Q. Wey
Summary: Managing the transmission of antimicrobial resistance requires reliable methods for tracking outbreaks. MALDI-TOF MS was compared with orthogonal approaches for strain typing, including WGS and FTIR, for Acinetobacter baumannii isolates associated with a healthcare-associated infection event. Reliable molecular typing methods can help to trace transmission routes and manage outbreaks.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Liangqiang Chen, Wenjing Gao, Xue Tan, Ying Han, Fu Jiao, Bin Feng, Jinghang Xie, Bin Li, Huilin Zhao, Huabin Tu, Shaoning Yu, Li Wang
Summary: MALDI-TOF MS is a method that can be used to classify specific model microbiota with high accuracy. The actual MS spectrum of the model microbiota shows a specific spectral fingerprint, which enhances the specificity and accuracy of microbiota classification.
MICROBIOLOGY SPECTRUM
(2023)
Article
Agricultural Engineering
Juana Rozalen, M. Mercedes Garcia, Amaya Zalacain, Horacio Lopez-Corcoles, Jorge Hurtado de Mendoza, Katrina Cornish, Manuel Carmona
Summary: Guayule, as a promising renewable source of natural rubber/latex, has resins with various activities potentially related to sesquiterpenes. By exploring different analytical methods, it was found that there may be new members of the guayulins family and the relationship in seasonal changes was identified.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Infectious Diseases
Natalia Kehl Moreira, Camila Morschbacher Wilhelm, Ayme Duarte Echevarria, Fabiana Caroline Zempulski Volpato, Priscila Lamb Wink, Afonso Luis Barth, Juliana Caierao
Summary: Detection of KPC enzyme directly from positive blood cultures using MALDI-TOF MS is feasible and has high sensitivity and specificity. This method can rapidly and accurately assess bacterial resistance mechanisms, which is crucial for selecting appropriate treatment and improving antimicrobial stewardship.
Review
Environmental Sciences
Mohammad Y. Ashfaq, Dana A. Da'na, Mohammad A. Al-Ghouti
Summary: Bacteria play diverse roles in the environment, with MALDI-TOF MS technology gaining attention for routine bacterial identification. This technique has been applied in various environmental studies, showing benefits in rapid and cost-effective bacteria identification.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Microbiology
Adriana Calderaro, Mirko Buttrini, Benedetta Farina, Sara Montecchini, Monica Martinelli, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto
Summary: The epidemiology of Clostridioides difficile infection (CDI) has changed over the past two decades, with the emergence of clinically relevant C. difficile strains causing severe nosocomial outbreaks. This study reports on an outbreak that occurred in a Long-term Care Unit, which was tracked using a Matrix-Assisted Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) typing approach (T-MALDI). The study also characterized the toxigenic and antimicrobial susceptibility profiles of the C. difficile isolates. The results showed the presence of three different clusters, with one cluster being considered epidemic and susceptible to vancomycin and metronidazole but resistant to moxifloxacin. This is the first reported outbreak associated with a tcdA+/tcdB-/cdt+ genotypic profile. T-MALDI is shown to be a valid and rapid approach for detecting and tracking outbreaks.
Article
Biochemistry & Molecular Biology
Chia-Ru Chung, Hsin-Yao Wang, Po-Han Chou, Li-Ching Wu, Jang-Jih Lu, Jorng-Tzong Horng, Tzong-Yi Lee
Summary: This study used an ensemble of multiple preprocessing methods to extract critical information from complicated mass spectrometry spectral data for identifying microorganisms and predicting antibiotic resistance. The ensemble method outperformed individual methods, achieving the highest accuracy on independent testing datasets. Important peaks related to antibiotic resistance could be detected, providing valuable information for further investigation of the resistance mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Claudia Honsig, Brigitte Selitsch, Marlene Hollenstein, Matthias G. Vossen, Kathrin Spettel, Birgit Willinger
Summary: In this study, the identification of 230 filamentous fungi isolates using MALDI-TOF MS in combination with the Filamentous Fungi Library 3.0 was investigated. The results showed that using the more time-consuming sample preparation methods improved species-level identification rates, and the in-house threshold for species identification was validated for routine use in the laboratory. This suggests that this commercial platform is suitable for routine diagnosis.
Article
Chemistry, Organic
Bernard Fernandez, Jean Armengaud, Gilles Subra, Christine Enjalbal
Summary: This study investigates the feasibility of using high energy charge remote fragmentations to analyze the N-terminus of proteins, and compares it with low energy collision-induced dissociations. The results show that the amino acid composition significantly affects the dissociation pathways of peptide backbones.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
