Article
Agriculture, Dairy & Animal Science
Peng Zheng, He Huang, Xiaoyu Li, Fushuo Huang, Samson Olugbenga Adeniran, Ziming Wang, Rui Feng, Guixue Zhang
Summary: This study aimed to improve pregnancy rates of dairy cows during timed artificial insemination (TAI) by using LRH-A3 and HCG. Results showed that while TAI did not affect pregnancy rates, LRH-A3 and HCG increased pregnancy rates in cows. HCG treatment affected gene expressions in endometrial cells.
ANIMAL SCIENCE JOURNAL
(2021)
Article
Agriculture, Dairy & Animal Science
C. M. Tippenhauer, J. -l. Plenio, W. Heuwieser, S. Borchardt
Summary: The objective of this observational study was to evaluate the association between increased physical activity at first artificial insemination (AI) and subsequent pregnancy per AI (P/AI) in lactating Holstein cows following spontaneous estrus or a timed AI (TAI) protocol. The study found that high intensity physical activity was positively correlated with pregnancy rate, suggesting that increased physical activity at the time of AI may improve fertility in cows. Rating: 8/10.
JOURNAL OF DAIRY SCIENCE
(2023)
Review
Agriculture, Dairy & Animal Science
P. M. Fricke, M. C. Wiltbank
Summary: The Ovsynch protocol has been proven to increase pregnancy per artificial insemination (P/AI) in lactating dairy cows, compared to cows inseminated after a detected estrus. Implementing timed artificial insemination (TAI) programs has been an important factor in the significant improvement of reproductive performance in lactating dairy cows in the United States over the past 20 years.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
D. P. Walsh, A. G. Fahey, P. Lonergan, M. Wallace
Summary: This study used a stochastic simulation model to estimate the potential economic benefit of using timed artificial insemination (TAI) in combination with conventional unsorted and sexed semen in a high-producing, pasture-based production system. The study found that TAI and sexed semen are complementary technologies that can increase genetic gain and profitability in a dairy production system.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
M. H. C. Pereira, F. R. Lopes Jr, A. K. Munhoz, K. G. Pohler, R. V. O. Filho, B. Cappellozza, J. L. M. Vasconcelos
Summary: This study aimed to compare the effects of different lengths of ovulation synchronization protocols on ovarian dynamics and pregnancy outcomes in lactating dairy cows, with the 10-day protocol showing higher pregnancy per artificial insemination rates in non-heat-stressed conditions compared to the 9-day protocol.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
C. M. Tippenhauer, J-L Plenio, A. M. L. Madureira, R. L. A. Cerri, W. Heuwieser, S. Borchardt
Summary: The observational study aimed to identify factors associated with estrous duration and intensity in lactating Holstein cows, finding a strong correlation between DU and PA, with cows exhibiting high PA having greater odds of pregnancy. Increased temperature-humidity index, short interestrus intervals, and changes in rumination were associated with DU and PA.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
M. Hoelper, L. Bretzinger, F. Randi, W. Heuwieser, S. Borchardt
Summary: This study evaluated the effects of 3 different Ovsynch protocols on progesterone and pregnancies per artificial insemination (P/AI). The results showed that a double dose of PGF2 alpha treatment led to decreased progesterone and increased P/AI. The presence of a corpus luteum influenced the treatment effect, and vaginal discharge caused by the PRID did not negatively impact fertility.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Reproductive Biology
R. Nunez-Olivera, G. A. Bo, A. Menchaca
Summary: This study evaluated the relationship between proestrus length and follicular size, estrous behavior, and pregnancy rate. The results showed that extending proestrus length could increase the diameter of the preovulatory follicle, proportion of heifers expressing estrus, and pregnancy rate in beef heifers.
Article
Agriculture, Dairy & Animal Science
C. Clabby, A. Valldecabres, P. Dillon, S. McParland, S. Arkins, K. O'Sullivan, J. Flynn, J. Murphy, P. Silva Bolona
Summary: This observational study aimed to explore the association between milk somatic cell count (SCC) and subclinical intramammary infection (IMI) and determine the optimal SCC cut-point for diagnosing IMI. The study found that in Irish pasture-based dairy herds with low bulk tank SCC, the last test-day SCC was the best predictor of IMI in late lactation.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Thabang Luther Magopa, Masindi Lottus Mphaphathi, Thendo Mulaudzi
Summary: The study compared oestrous synchronization expression and conception rate following timed artificial insemination (TAI) with frozen-thawed X-sexed or unsexed semen in dairy and beef cows. The results showed that overall, dairy cows had higher conception rates on Day 35 compared to beef cows. Dairy cows also had higher incidence of pregnancy losses between Days 66 and 95 when compared to beef cows.
REPRODUCTION IN DOMESTIC ANIMALS
(2023)
Article
Reproductive Biology
Alejandro M. Rodriguez, Sebastian Maresca, Sebastian Lopez-Valiente, Maria G. Bilbao, Karen D. Moran, Julian A. Bartolome, Scott L. Pratt, Nathan M. Long
Summary: Two experiments were conducted to compare the effects of two different synchronization protocols on follicle diameter, corpus luteum area, progesterone concentration, pregnancy per timed artificial insemination (TAI), and pregnancy loss in cows. The results showed no significant differences in pregnancy per TAI and pregnancy loss between cows using 7-d CO-Synch + PIVD or 8-d estradiol-based + PIVD protocols.
Article
Reproductive Biology
Ill-Hwa Kim, Jae-Kwan Jeong, Hyun-Gu Kang
Summary: This study aims to identify the factors influencing reproductive outcomes in lactating dairy cows. Factors such as nutrition, health, timing of artificial insemination, and weather were found to affect the probability of pregnancy. High body condition score and appropriate timing of artificial insemination increase the likelihood of pregnancy. Heat stress, pre- or postpartum disorders, and longer time to first artificial insemination have a negative impact on pregnancy outcomes.
Article
Agriculture, Dairy & Animal Science
J. P. N. Martins, T. O. Cunha, W. Martinez, J. S. Schmitt
Summary: This study aimed to compare the effects of two breeding strategies, Artificial Insemination after Detection of Estrus (AIED) and Timed Artificial Insemination (TAI), on first-service fertility in lactating Holstein cows. The results showed that the PG+G strategy resulted in a higher proportion of cows receiving TAI and a longer time to first service compared to the Presynch-14 strategy. Additionally, the PG+G strategy also led to a higher ovulatory response and proportion of cows with functional corpora lutea. Overall, combining TAI with AIED and using the PG+G strategy improved pregnancy rates and calving rates per AI compared to using the Presynch-14 strategy.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
A. M. Hubner, I. F. Canisso, P. M. Peixoto, W. M. Coelho, L. L. Cunha, L. Ribeiro, S. Crump, F. S. Lima
Summary: This study investigated the effects of nerve growth factor-0 (NGF) from bulls' seminal plasma on progesterone, interferon-stimulated genes (ISG), and pregnancy per artificial insemination (AI) in lactating Holstein dairy cows. The results showed that NGF did not significantly affect progesterone, ISG abundance, or pregnancy rate.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Reproductive Biology
P. Taaffe, C. M. O'Meara, M. Stiavnicka, C. J. Byrne, B. Eivers, P. Lonergan, S. Fair
Summary: This study aimed to assess the effect of ejaculate collection frequency on semen output, sperm quality, and field fertility in young bulls. The results showed that collecting ejaculates more frequently significantly reduced the number of days required to obtain 1000 straws, increased semen quality in terms of lower superoxide production, and improved field fertility.
Article
Agriculture, Dairy & Animal Science
R. S. Bisinotto, E. S. Ribeiro, L. F. Greco, D. Taylor-Rodriguez, A. D. Ealy, H. Ayres, F. S. Lima, N. Martinez, W. W. Thatcher, J. E. P. Santos
Summary: This study evaluated the effects of follicular wave and progesterone concentration on growth of the ovulatory follicle, conceptus elongation, uterine IFN-tau concentration, and transcriptome of conceptus and endometrium in pregnant cows. The results indicated that progesterone concentration had an impact on the growth of ovulatory follicle, but overall had minimal effects on the conceptus and endometrium transcriptome.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Veterinary Sciences
Jamie L. Stewart, Liying Gao, Jodi A. Flaws, Vitor R. G. Mercadante, Nicholas W. Dias, Igor F. Canisso, Fabio S. Lima
Summary: This study assessed the direct effects of purified bovine NGF on steroidogenesis and angiogenic markers in the bovine pre-ovulatory follicle. The results showed that NGF can stimulate testosterone production in the follicle wall cells and accelerate the cellular remodeling that occurs during early luteal development.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
I. J. Lean, S. J. LeBlanc, D. B. Sheedy, T. Duffield, J. E. P. Santos, H. M. Golder
Summary: Data from three countries were analyzed to evaluate the association between parity and disease in Holstein cows. The study found that higher parity was associated with increased disease risk and changes in metabolite concentrations.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Developmental Biology
Anthony D. Horlock, Thomas J. R. Ormsby, Martin J. D. Clift, Jose E. P. Santos, John J. Bromfield, I. Martin Sheldon
Summary: This study demonstrates the role of cholesterol in supporting inflammatory responses to LPS in bovine granulosa cells. Serum or follicular fluid containing cholesterol enhances the secretion of IL-1 alpha and IL-1 beta in granulosa cells stimulated with LPS, while depleting cholesterol diminishes this effect. Furthermore, high-density lipoprotein cholesterol and cholesterol biosynthesis pathway are also involved in granulosa cell inflammation.
Article
Genetics & Heredity
Zuoxiang Liang, Dzianis Prakapenka, Kristen L. Parker Gaddis, Michael J. VandeHaar, Kent A. Weigel, Robert J. Tempelman, James E. Koltes, Jose Eduardo P. Santos, Heather M. White, Francisco Penagaricano, Ransom L. Baldwin, Yang Da
Summary: This study evaluated the impact of genomic epistasis effects on the accuracy of predicting the phenotypic values of residual feed intake in U.S. Holstein cows. The results showed that the combination of AxA epistasis and additive effects in the prediction model improved the accuracy of predictions.
FRONTIERS IN GENETICS
(2022)
Article
Agriculture, Dairy & Animal Science
Thiago Martins, Cecilia C. Rocha, Joseph Danny Driver, Owen Rae, Mauricio A. Elzo, Raluca G. Mateescu, Jose Eduardo P. Santos, Mario Binelli
Summary: This study found that an increased proportion of B. indicus genetics in crossbred cows resulted in reduced estrous response and pregnancy per artificial insemination after estrous synchronization. However, it did not lower the proportion of pregnant cows at the end of the breeding season. The interval from the beginning of the breeding season to pregnancy was extended as the proportion of B. indicus genetics in cows increased.
JOURNAL OF ANIMAL SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
M. B. Poindexter, R. Zimpel, A. Vieira-Neto, A. Husnain, A. C. M. Silva, A. Faccenda, A. Sanches de Avila, P. Celi, C. Cortinhas, J. E. P. Santos, C. D. Nelson
Summary: The objectives of this experiment were to determine the effects of supplementing 25-hydroxyvitamin D3 compared with vitamin D3 at 1 or 3 mg/d in late gestation on production outcomes of dairy cows. The results showed that feeding 25-hydroxyvitamin D3 increased colostrum and milk yield compared to vitamin D3. The effect of 25-hydroxyvitamin D3 on milk yield may be related to serum calcium concentrations.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
I. J. Lean, H. M. Golder, S. J. LeBlanc, T. Duffield, J. E. P. Santos
Summary: In this retrospective meta-analysis, individual cow data was used to assess the associations between parity, level of production, and pasture-based or intensively fed systems with fertility. The study found that parity and milk production measures had significant effects on reproductive outcomes. Differences in production systems also influenced reproductive measures.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
Z. B. Turner, F. S. Lima, A. J. Conley, B. R. McNabb, J. D. Rowe, A. Garzon, T. M. Urbano, C. M. Morris, R. V. Pereira
Summary: The most frequent definition for cystic ovarian disease in cattle is an abnormally persistent follicle (>7 to 10 d) with a diameter >25 mm. Discriminating between luteal and follicular ovarian cystic structures has traditionally been done by measuring the rim width of luteal tissue. The most common practice for diagnosing cystic ovarian disease is rectal palpation with or without the use of a B-mode ultrasound.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
U. Arshad, A. Husnain, M. B. Poindexter, R. Zimpel, M. C. Perdomo, J. E. P. Santos
Summary: The objective of this study was to investigate the effect of increasing supplementation of rumen-protected choline (RPC) on hepatic metabolism in cows. The results showed that supplementation of RPC reduced hepatic triacylglycerol and increased glycogen content. Additionally, RPC supplementation had an impact on serum haptoglobin levels, but did not affect the concentrations of fatty acids, BHB, glucose, triacylglycerol, and total cholesterol in the blood.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Reproductive Biology
Ali Husnain, Usman Arshad, Roney Zimpel, Eduardo Schmitt, Mackenzie J. Dickson, Milerky C. Perdomo, Mariana N. Marinho, Nadia Ashrafi, Stewart F. Graham, Jeanette Bishop, Thomas R. Hansen, Kwang C. Jeong, Angela M. Gonella-Diaza, Ricardo C. Chebel, I. Martin Sheldon, John J. Bromfield, Jose E. P. Santos
Summary: Endometrial inflammation in cows is associated with reduced pregnancy rates and increased pregnancy loss. Induced endometritis alters histotroph composition and induces inflammatory signatures on conceptus, compromising their development. Bacterial-induced endometritis impairs conceptus development by altering histotroph composition and conceptus gene expression in dairy cattle.
BIOLOGY OF REPRODUCTION
(2023)
Review
Agriculture, Dairy & Animal Science
T. C. Marques, C. F. A. Lage, D. R. Bruno, E. D. Fausak, M. I. Endres, F. C. Ferreira, F. S. Lima
Summary: The adoption of Automatic Milking System (AMS) in the United States is increasing due to factors such as lower availability and increased cost of labor. The new generation of farmers is also showing more interest in precision dairy farming. Most of the research on non-pasture-based AMS is conducted in Europe, with variations in the breeds of cows, brands of AMS used, and traffic flow. The main research topics focus on milk production, milk composition, AMS efficiency, as well as behavior and welfare, health disorders, and nutrition. In the United States, the trends are similar to Europe, except for nutrition. Future research should focus on reproductive management, other health disorders, economics, and water and energy consumption optimization.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Agriculture, Dairy & Animal Science
S. Casaro, J. G. Prim, T. D. Gonzalez, C. C. Figueiredo, R. S. Bisinotto, R. C. Chebel, J. E. P. Santos, C. D. Nelson, S. J. Jeon, R. C. Bicalho, J. P. Driver, K. N. Galvao
Summary: The objective of this study was to identify metabolites associated with metritis and investigate the cellular mechanisms affected during the transition into lactation. The results showed significant changes in metabolism, lipolysis, cell death, oxidative stress, and immune activation in cows with metritis, indicating a predisposition to metritis development.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Eduardo B. de Oliveira, Hugo F. Monteiro, Jessica M. V. Pereira, Deniece R. Williams, Richard V. Pereira, Noelia Silva Del Rio, Paulo R. Menta, Vinicius S. Machado, Fabio S. Lima, Bruno Stefanon
Summary: The objective of this study was to identify potential biomarkers for predicting the development and cure of metritis in dairy cows by analyzing the metabolome of vaginal discharge. The study found that metritis was associated with changes in the uterine metabolome, and metabolome analysis of vaginal discharge could be an important tool for understanding postpartum vaginal discharge changes and the dynamics of metritis development and cures.
Article
Microbiology
Carl Basbas, Adriana Garzon, Cory Schlesener, Machteld van Heule, Rodrigo Profeta, Bart C. Weimer, Noelia Silva-del-Rio, Barbara A. Byrne, Betsy Karle, Sharif S. Aly, Fabio S. Lima, Richard V. Pereira
Summary: This study assessed the microbial ecology and diversity in the uterus of post-partum dairy cows with and without metritis using shotgun metagenomics. The results showed that cows without metritis had increased alpha-diversity and decreased beta-diversity compared to cows with metritis. Bacteroides, Porphyromonas, and Fusobacterium were found to be more abundant in cows with metritis compared to cows without metritis.