Article
Biochemistry & Molecular Biology
Iara Aime Cardoso, Aline Kusumota Luiz de Souza, Adam Muslem George Burgess, Iain Wyllie Chalmers, Karl Francis Hoffmann, Maria Cristina Nonato
Summary: Schistosomiasis is a neglected tropical disease affecting over 250 million people globally, and there is an urgent need for new therapies due to limitations of current treatments. Fumarases in Schistosoma mansoni have been identified as potential targets for developing novel treatments. The structural characterization of fumarases provides a basis for rational search for selective ligands.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Gastroenterology & Hepatology
Valerio Azzimato, Ping Chen, Emelie Barreby, Cecilia Morgantini, Laura Levi, Ana Vankova, Jennifer Jager, Andre Sulen, Marina Diotallevi, Joanne X. Shen, Anne Miller, Ewa Ellis, Mikael Ryden, Erik Naslund, Anders Thorell, Volker M. Lauschke, Keith M. Channon, Mark J. Crabtree, Arvand Haschemi, Siobhan M. Craige, Mattia Mori, Francesco Spallotta, Myriam Aouadi
Summary: This study reveals a novel mechanism by which miR-144 inhibits NRF2 activity through the consumption of fumarate by activation of FH. By activating fumarate hydratase in the TCA cycle, hepatic miR-144 triggers an impaired antioxidant response in obesity.
Article
Biochemistry & Molecular Biology
Ping Li, Yanru Wu, Huizhi Wu, Qiuhong Xiong, Na Zhao, Guangxin Chen, Changxin Wu, Han Xiao
Summary: This study identified a mutation in the FH gene that is associated with uterine leiomyomas and investigated its pathogenicity. The FH mutation exhibited lower enzyme activity and higher fumarate levels, possibly due to impaired homotetramer formation. Additionally, the mutation led to increased cell proliferation and extracellular acidification, as well as inhibited autophagy. These findings provide important insights for accurate genetic counseling and clinical diagnosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Mika Takeuchi, Yutaka Amao
Summary: Fumarase from porcine heart is an important enzyme in the visible-light driven NADH regeneration system, and its interaction with water-soluble zinc porphyrin can affect fumarate production efficiency.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Yardena Silas, Esti Singer, Koyeli Das, Norbert Lehming, Ophry Pines
Summary: Class-II fumarases are dual-targeted enzymes found in the mitochondria and cytosol of all eukaryotes, playing a crucial role in protecting cells from DNA double-strand breaks. The gram-positive bacterium Bacillus subtilis' Class-II fumarase participates in both the tricarboxylic acid cycle and DNA damage response.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Physical
Guosi Li, Fucheng Zhu, Fangli Gu, Xinjian Yin, Qilin Xu, Menghua Ma, Li Zhu, Baowei Lu, Naidong Chen
Summary: In this study, immobilized fumarase within konjac-K-carrageenan beads was used to improve the conversion and yield of calcium fumarate, achieving the highest reported value. The beads showed good reusability and stability.
Article
Biochemistry & Molecular Biology
Alexander T. Eprintsev, Dmitry N. Fedorin, Mikhail V. Cherkasskikh, Abir U. Igamberdiev
Summary: Salt stress significantly affects the gene expression, promoter methylation, and enzymatic activity of aconitase and fumarase forms in maize seedlings. Mitochondrial forms of the enzymes showed increased activity while cytosolic forms decreased under salt stress, correlating with promoter methylation levels. This epigenetic regulation of aconitase and fumarase isoforms plays a key role in response to salt stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Miika Mehine, Terhi Ahvenainen, Sara Khamaiseh, Jouni Harkonen, Siiri Reinikka, Tuomas Heikkinen, Anna Ayravainen, Paivi Pakarinen, Paivi Harkki, Annukka Pasanen, Anna-Liisa Levonen, Ralf Butzow, Pia Vahteristo
Summary: A novel subtype of uterine leiomyomas has been identified, characterized by distinct molecular features, mutations disrupting neddylation of the Cullin 3-RING E3 ligase, and activation of the NRF2 pathway. These findings provide new opportunities for targeted treatment of uterine leiomyomas.
Article
Multidisciplinary Sciences
Hsin-Hua Wu, Michael D. Pun, Courtney E. Wise, Bennett R. Streit, Florence Mus, Anna Berim, William M. Kincannon, Abdullah Islam, Sarah E. Partovi, David R. Gang, Jennifer L. DuBois, Carolyn E. Lubner, Clifford E. Berkman, B. Markus Lange, John W. Peters
Summary: The article introduces the complete CoM biosynthesis pathway in bacteria, revealing distinct chemical steps compared to CoM biosynthesis in methanogenic archaea, which represents a profound instance of convergent evolution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Luis Cervela-Cardona, Takuya Yoshida, Youjun Zhang, Masaaki Okada, Alisdair Fernie, Paloma Mas
Summary: The Arabidopsis clock component TOC1 plays a crucial role in the diurnal regulation of metabolism by repressing the expression of the TCA-related gene FUMARASE 2 at night. This regulation affects the accumulation of fumarate and contributes to the molecular and physiological energy-deprivation phenotypes of TOC1 over-expressing plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Pablo Hernandez-Ortego, Remedios Torres-Montero, Elvira de la Pena, Felix Viana, Jorge Fernandez-Trillo
Summary: TRPM8 is a non-selective cation channel widely expressed in primary sensory neurons and other tissues. This study characterized the performance of six commercial TRPM8 antibodies in three immunodetection techniques and found that two antibodies were successful in detecting TRPM8 in all approaches, while the other four were only acceptable for specific techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pathology
Qiu-ju Miao, Jie Zang, Xue-Bao Shao, Jian-fang Sun, Yan-Ping Chen, Hao Chen
Summary: PRAME is a reliable immunohistochemistry marker for distinguishing melanoma from other lesions. This study investigated PRAME expression in acral lentiginous melanoma in situ (ALMIS) and subungual melanoma in situ (SMIS), and found high sensitivity and specificity for diagnosing these types of melanoma.
JOURNAL OF CLINICAL PATHOLOGY
(2023)
Article
Engineering, Environmental
Sita Shrestha, Seo Yeon Lee, Devendra Shrestha, Rupesh Kandel, Yeo-Jin Yoo, Hyun-Jin Tae, Bishnu Kumar Shrestha, Chan Hee Park, Cheol Sang Kim
Summary: This work describes the synthesis of an ideal bioceramic that closely resembles bone-like apatite in geometry and composition, and can easily stimulate cellular response. The bioceramic, with an optimized weight percentage of zinc, shows potential for bone defect repair and bone void filling in various clinical approaches. The detailed physicochemical and biological characterizations reveal that the bioceramic possesses a biocompatible and porous structure similar to hydroxyapatite, and stimulates bone regeneration by enhancing cell viability and osteogenic differentiation. The findings also demonstrate the effectiveness of the bioceramic in promoting new bone growth in a rat model with critical size calvarial defect. This study provides valuable insights into the osteogenesis of the (Ti(HPO4)(2)/Zn-3(PO4)(2)·nH2O) bioceramic and contributes to the development of therapeutic strategies in bone tissue regenerative medicine.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Plant Sciences
Fareen Sami, Husna Siddiqui, Shamsul Hayat
Summary: The study shows that foliar spray of sodium nitroprusside (SNP) on mustard plants can enhance photosynthetic efficiency, carbon metabolism, nutrient status, and activity of various enzymes related to nitrogen metabolism, CO2/HCO(3)(-) homeostasis, glycolysis, Calvin cycle, and Krebs cycle. Additionally, SNP application reduces reactive oxygen species and lipid peroxidation, while upregulating the antioxidative defense system.
JOURNAL OF PLANT GROWTH REGULATION
(2021)
Article
Biochemical Research Methods
Elisabet Rosas-Canyelles, Andrew J. Modzelewski, Alisha Geldert, Lin He, Amy E. Herr
Summary: A novel technique (snapBlot) has been introduced for simultaneous measurement of protein isoforms and nucleic acids from the same embryo, with the potential to uncover the regulatory mechanisms of nucleic acids in mammalian embryo development.
Letter
Dermatology
Titus J. Brinker, Max Schmitt, Eva I. Krieghoff-Henning, Raymond Barnhill, Helmut Beltraminelli, Stephan A. Braun, Richard Carr, Maria-Teresa Fernandez-Figueras, Gerardo Ferrara, Sylvie Fraitag, Raffaele Gianotti, Mar Llamas-Velasco, Cornelia S. L. Mueller, Antonio Perasole, Luis Requena, Omar P. Sangueza, Carlos Santonja, Hans Starz, Esmeralda Vale, Wolfgang Weyers, Achim Hekler, Jakob N. Kather, Stefan Froebling, Dieter Krahl, Tim Holland-Letz, Jochen S. Utikal, Andrea Saggini, Heinz Kutzner
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY
(2022)
Article
Dermatology
A. Catala, C. Munoz-Santos, C. Galvan-Casas, M. Roncero Riesco, D. Revilla Nebreda, A. Sola-Truyols, P. Giavedoni, M. Llamas-Velasco, C. Gonzalez-Cruz, X. Cubiro, R. Ruiz-Villaverde, S. Gomez-Armayones, M. P. Gil Mateo, D. Pesque, O. Marcantonio, D. Fernandez-Nieto, J. Romani, N. Iglesias Pena, L. Carnero Gonzalez, J. Tercedor-Sanchez, G. Carretero, T. Masat-Tico, P. Rodriguez-Jimenez, A. M. Gimenez-Arnau, M. Utrera-Busquets, E. Vargas Laguna, A. G. Angulo Menendez, E. San Juan Lasser, M. Iglesias-Sancho, L. Alonso Naranjo, I Hiltun, E. Cutillas Marco, I Polimon Olabarrieta, S. Marinero Escobedo, X. Garcia-Navarro, M. J. Calderon Gutierrez, G. Baeza-Hernandez, L. Bou Camps, T. Toledo-Pastrana, A. Guilabert
Summary: This study investigated cutaneous reactions after SARS-CoV-2 vaccination, finding that most were mild to moderate, but severe cases were also reported. Understanding these reactions can help healthcare professionals and reassure patients.
BRITISH JOURNAL OF DERMATOLOGY
(2022)
Article
Dermatology
Raquel Navarro, Yolanda Delgado-Jimenez, Juan Guinea-Viniegra, Mar Llamas-Velasco, Esteban Dauden
Summary: The study found that the expression profiles of miR-21 and TIMP-3 differ between induced psoriasis and non-induced psoriasis, with most cases of paradoxical psoriasiform reactions showing high TIMP-3 expression, while the control group showing different patterns. This suggests that various pro-inflammatory cytokines are involved in the pathogenesis of paradoxical psoriasiform reactions and non-induced psoriasis.
JOURNAL OF CUTANEOUS PATHOLOGY
(2022)
Article
Dermatology
Elena del Alcazar, Anna Lopez-Ferrer, Alvaro Martinez-Domenech, Ricardo Ruiz-Villaverde, Ma. del Mar Llamas-Velasco, Vicenc Rocamora, Marc Julia, Jaime Notario, Lourdes Rodriguez Fernandez-Freire, Antonio Sahuquillo-Torralba, David Vidal, Raquel Rivera, Gregorio Carretero, Almudena Mateu, Pablo de la Cueva, Jose Manuel Carrascosa
Summary: This study aimed to assess the effectiveness and safety of guselkumab in the treatment of moderate to severe plaque psoriasis in routine clinical practice. The results showed that guselkumab had significant efficacy and good safety profile at 24 weeks.
DERMATOLOGIC THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Amalia Lamana, David Castro-Vazquez, Hortensia de la Fuente, Ana Triguero-Martinez, Rebeca Martinez-Hernandez, Marcelino Revenga, Raul Villanueva-Romero, Mar Llamas-Velasco, Pablo Chicharro, Yasmina Juarranz, Monica Marazuela, Marco Sales-Sanz, Rosario Garcia-Vicuna, Eva Tomero, Isidoro Gonzalez-Alvaro, Carmen Martinez, Rosa P. Gomariz
Summary: This study investigated the expression of vasoactive intestinal peptide (VIP) and its receptors in immune-mediated inflammatory disorders (IMID), and identified miRNA signatures associated with their expression. The results suggest that the expression of VIP/VPAC2 may serve as markers for IMID and provide insights into the role of the VIP/VPAC axis and miRNA signatures in immune-mediated diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Letter
Dermatology
A. Reymundo, E. Vilarrasa, O. Baniandres, L. Rodriguez-Fernandez-Freire, R. Feltes, M. Llamas-Velasco, E. Dauden
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2022)
Article
Dermatology
Pablo Chicharro, Mar Llamas-Velasco, Susana Armesto, Enrique Herrera Acosta, David Vidal, Eva Vilarrasa, Raquel Rivera-Diaz, Pablo De-la-Cueva, Antonio Martorell-Calatayud, Ferran Ballesca, Isabel Belinchon, Gregorio Carretero, Lourdes Rodriguez, Alberto Romero-Mate, Josep Pujol-Montcusi, Laura Salgado, Antonio Sahuquillo-Torralba, Pablo Coto-Segura, Ofelia Baniandres Rodriguez, Rosa Feltes, Jose Riera-Monroig, Esteban Dauden
Summary: The study assessed the long-term effectiveness and safety of secukinumab in Spanish patients with moderate-to-severe psoriasis in a daily practice setting, finding that the drug was particularly effective for patients with high PSI and had good long-term safety in routine clinical practice.
DERMATOLOGIC THERAPY
(2022)
Article
Dermatology
Vicenc Rocamora, Laura Crespi, Marta Ferran, Mar Llamas-Velasco, Elena del Alcazar, Jose M. Carrascosa, Emma Beltran, Ana Urruticoechea-Arana, Jose Luis Lopez Estebaranz, David Vidal, Josep Riera, Lourdes Rodriguez, Susana Armesto, Josep M. Fernandez, Gloria Aparicio, Silvia Perez, Saray Porcar, Encarna Montesinos, Fernando Gallardo
Summary: This study evaluated the effectiveness and tolerability of Guselkumab in the treatment of psoriatic arthritis in clinical practice. The results showed that Guselkumab effectively controlled both joint and skin manifestations. No new safety concerns were found, and the majority of patients continued the treatment after 2 years of follow-up.
DERMATOLOGIC THERAPY
(2022)
Article
Health Care Sciences & Services
Alejandra Reolid, Antonio Sahuquillo-Torralba, Ancor Sanz-Garcia, Rafael Botella-Estrada, Ester Munoz-Aceituno, Mar Llamas-Velasco, Jorge Garcia-Martinez, Esteban Dauden, Francisco Abad-Santos, Maria C. Ovejero-Benito
Summary: This study aims to identify DNA copy number variations (CNVs) associated with different clinical phenotypes of psoriasis, and to explore the genetic background of anti-TNF-induced palmoplantar pustulosis (PPP). The results suggest that certain genes may play a role in the development of anti-TNF-induced PPP.
JOURNAL OF PERSONALIZED MEDICINE
(2022)
Editorial Material
Dermatology
B. Butron-Bris, I. Llado, L. Martos-Cabrera, R. Sampedro, J. Fraga, P. Rodriguez-Jimenez, M. Llamas-Velasco
JOURNAL OF CUTANEOUS PATHOLOGY
(2023)
Article
Dermatology
Antonio Costanzo, Mar Llamas-Velasco, Gabriella Fabbrocini, Aldo Cuccia, Raquel Rivera-Diaz, Kristian Gaarn Du Jardin, Ismail Kasujee, Lluis Puig, Jose Manuel Carrascosa
Summary: The TRIBUTE study assessed the efficacy and impact on health-related quality of life of Tildrakizumab in moderate-to-severe psoriasis patients in conditions similar to clinical practice. After 24 weeks, a high proportion of patients achieved significant improvement in psoriasis signs and quality of life. Patient-reported outcomes showed improvements in sleep outcomes, work productivity, and treatment satisfaction. The safety profile was favorable.
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Article
Oncology
Alexander Kurz, Dieter Krahl, Heinz Kutzner, Raymond Barnhill, Antonio Perasole, Maria Teresa Fernandez Figueras, Gerardo Ferrara, Stephan A. Braun, Hans Starz, Mar Llamas-Velasco, Jochen Sven Utikal, Stefan Froehling, Christof von Kalle, Jakob Nikolas Kather, Lucas Schneider, Titus J. Brinker
Summary: This study developed the first human 3D melanoma histology model with complete data and code availability. Through testing and discussion, it is found that 3D histology models have potential applications in digital pathology, but there are still limitations that need to be addressed.
EUROPEAN JOURNAL OF CANCER
(2023)
Article
Dermatology
M. Llamas-Velasco, K. Kiss, L. Melchior, T. Mentzel
JOURNAL OF CUTANEOUS PATHOLOGY
(2023)
Letter
Dermatology
A. Reymundo, S. Armesto, L. Rodriguez, O. Baniandres, A. Sahuquillo-Torralba, T. Torres, P. de la Cueva, M. Llamas-Velasco, E. Dauden
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)
Letter
Dermatology
M. Menendez Sanchez, A. Muniz de Lucas, E. Perez Fernandez, M. Llamas Velasco, D. P. Ruiz Genao, J. L. Lopez Estebaranz
JOURNAL OF THE EUROPEAN ACADEMY OF DERMATOLOGY AND VENEREOLOGY
(2023)